DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CG30456 and Arhgef25

DIOPT Version :9

Sequence 1:	NP_611188.3	Gene:	CG30456 / 36929	FlyBaseID:	FBgn0050456	Length:	682	Species:	Drosophila melanogaster
Sequence 2:	NP_082303.2	Gene:	Arhgef25 / 52666	MGIID:	1277173	Length:	618	Species:	Mus musculus

Alignment Length:	380	Identity:	98/380 - (25%)
Similarity:	165/380 - (43%)	Gaps:	87/380 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly   304 PKDEEEEVEIELRDKSDKCVHPILEELIKTEEAYVNNLFTGIENYGNIFQRKDLPLGLRGKKYDL 368
            ||:|||:.::.|.....     :|.||::||..||::|...:|.|......:.:|..|||:...:
Mouse   186 PKNEEEQKKMALERSMF-----VLGELVETERTYVDDLGQIVEGYMATMATQGVPESLRGRDRIV 245

  Fly   369 FGNIEQIAEFHRDEFLPMLQRNRRDLKRLFDEFLQFLDQHCFYGYVIFTMNKQKSLK-LCDLYKN 432
            ||||:||.|:|||.||..||:..:|...|...|::  .:...:.||::..||.||.. |.:...:
Mouse   246 FGNIQQIYEWHRDYFLQELQQCLKDPDWLAQLFIK--HERRLHMYVVYCQNKPKSEHVLSEFGDS 308

  Fly   433 YFTSIRLERDDKLGINSFLVQPIQRMARYPLLLTQFINTFFK-NRDI-VMKPLIESCCRLEKRLR 495
            ||..:|.:...:|.:|..|::|:||:.:|.|||..|:..:.: .:|. .::..:|..|.:.||
Mouse   309 YFEELRQQLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYRRAGKDTEELEQAVEVMCFVPKR-- 371

  Fly   496 ALLTTTNESEIINDIVDC-----HEFNVYYQGKFRKVNEFQVLDHKL-----KRSYRSKVFIFDK 550
                       .||::..     .|..:..|||....:.|.|.:.:.     .|....:||:|::
Mouse   372 -----------CNDMMSLGRLRGFEGKLTAQGKLLGQDTFLVTEPEAGGLLSSRGRERRVFLFEQ 425

  Fly   551 CIIYTEIKG-------------KNLLFHGRYPCEHIGISAKTKSFTLYYERRKQQECEF------ 596
            .||::|..|             ||.:   :..|  :|:....:.          ..|.|
Mouse   426 IIIFSEALGGGGRGGAQPGYVYKNSI---KVSC--LGLEGNLQG----------NPCRFALTSRG 475

  Fly   597 -----------TADPV-------QIAIWLDLIRDMINNYANEERQKLQERYSREN 633
                       .:||.       |:|..|:..||.:|  |.:...:.|.|.|:.|
Mouse   476 PEGGIQRYVLQASDPAVSQAWIKQVAQILESQRDFLN--ALQSPIEYQRRESQTN 528

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG30456	NP_611188.3	RhoGEF	326..470	CDD:279015	52/144 (36%)
Arhgef25	NP_082303.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	26..63
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	169..193		5/6 (83%)
		RhoGEF	203..374	CDD:279015	57/185 (31%)
		Important for binding to Rho GTPases. /evidence=ECO:0000250	317..338		6/20 (30%)
		PH2_Kalirin_Trio_p63RhoGEF	378..520	CDD:270061	29/158 (18%)
		Sufficient to bind activated GNAQ. /evidence=ECO:0000250	506..532		8/25 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	521..556		4/8 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	584..604
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	1	Normalized mean entropy	S5924
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR22826
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.860