DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and ARHGEF1

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_945353.1	Gene:	ARHGEF1 / 9138	HGNCID:	681	Length:	927	Species:	Homo sapiens

Alignment Length:	1273	Identity:	295/1273 - (23%)
Similarity:	465/1273 - (36%)	Gaps:	448/1273 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly   851 DPNENN---RGAAAAGPGVFIESHQFTPMAGASSP--IPISLHSSHMHAAQSNDTQKEIISMEDE 910
            :|.|..   ||||:.||               |.|  :|:|                 ||..|||
Human    12 EPREMEDFARGAASPGP---------------SRPGLVPVS-----------------IIGAEDE 44

  Fly   911 N--SDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDM 973
            :  ::|:....::|..|.:|.::  ......|...|.:|....:|.|||..|..::......|:.
Human    45 DFENELETNSEEQNSQFQSLEQV--KRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEA 107

  Fly   974 RKWAYEIHSTFL----VPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLIS 1034
            :|...:.:.:||    |.|.|:     ..::|.|:|                    |.|| ||||
Human   108 KKAFLDFYHSFLEKTAVLRVPV-----PPNVAFELD--------------------RTRA-DLIS 146

  Fly  1035 E--------------------QLREFQQKRTAGLGTIYGPTDDKLAEAK--------TDKLREQI 1071
            |                    ||.:|:.||..|:    .|.:.:||:.:        :.:.||:.
Human   147 EDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGM----TPWEQELAQLEAWVGRDRASYEARERH 207

  Fly  1072 IDKYLMPNL----HALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRD 1132
            :.:.|:.:|    |.:..||.       :..|:.:|:.  :|               :.|...|.
Human   208 VAERLLMHLEEMQHTISTDEE-------KSAAVVNAIG--LY---------------MRHLGVRT 248

  Fly  1133 KS---------FKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNI 1188
            ||         |:.::||..|                                     :|.....
Human   249 KSGDKKSGRNFFRKKVMGNRR-------------------------------------SDEPAKT 276

  Fly  1189 HRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQ 1253
            .:..|.::|         |.|.                                       :||:
Human   277 KKGLSSILD---------AARW---------------------------------------NRGE 293

  Fly  1254 ASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSV 1318
            ..:   |..|...|.:.....|          .||.:               ||.|
Human   294 PQV---PDFRHLKAEVDAEKPG----------ATDRK---------------GGVG--------- 321

  Fly  1319 ASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNR 1383
               .||                                                        |:|
Human   322 ---MPS--------------------------------------------------------RDR 327

  Fly  1384 NSRRKTSD-PSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQV 1447
            |......| |.:|..|      |.|.:......:::.|.....|.....|:|..|.|
Human   328 NIGAPGQDTPGVSLHP------LSLDSPDREPGADAPLELGDSSPQGPMSLESLAPP-------- 378

  Fly  1448 PPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDE 1512
                            :...:..:.:|.:.|..|..|.|.                   ||.|.|
Human   379 ----------------ESTDEGAETESPEPGDEGEPGRSG-------------------LELEPE 408

  Fly  1513 DDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQD 1577
            :   ...|..:|..:.|.:|..::.||||:|:|:..||..|||.|::|..|||.|:.|......:
Human   409 E---PPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLE 470

  Fly  1578 HLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQ 1642
            .|..:| |:|..|.|:|..|...|.:||.|..:::..|||:|...|||..|....:.:::||:||
Human   471 ELQNIF-PSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQ 534

  Fly  1643 QIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLP 1707
            ..|||.||.|:.||......::::||...||||:|||::||.:|||||||||.:::.:.|
Human   535 SFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNT----- 594

  Fly  1708 ENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLD----RSSYDK--EEFKKLDLT 1766
            |..||.|.::.|.|..:.||..|||||:..||..:|::.||:||    |.|.|.  .|||.||:|
Human   595 EEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDIT 659

  Fly  1767 QHHLIHDGNLT--IKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLH----TPLSITNKPVSPIM 1825
            :..|:|:|.||  :.|:.:|::|.||.:::::||.:||::..||: |    ||.......:.|::
Human   660 KKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKS-HSRTLTPTPDGKTMLRPVL 723

  Fly  1826 SIDADTLIRQEAADKNSFFLIKM--KTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASS 1888
            .: ...:.|:.|.|..:|:::..  :.:|:.||.|.:.||.|.|....::.|.
Human   724 RL-TSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAG------------ 775

  Fly  1889 NHDTSISDPALAAIPH-----SNTKESLELSTDT------VQPLAATATLTTTPLAPMLPIATVT 1942
                |:..||.|:.|.     |:|:|.|..|::.      ..|..|.   |...|:.:||..  .
Human   776 ----SLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADAR---TERILSDLLPFC--R 831

  Fly  1943 PAP-----ATNNSNVSSLTGVQLRNPQRDATASESDADYVNTP-----KPRSSQNEVNRTMSIRS 1997
            |.|     ||....|.||.  ||..|..:...:....|....|     .|..:|......:|:..
Human   832 PGPEGQLAATALRKVLSLK--QLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEE 894

  Fly  1998 TGEPIQKYSANGTEANDVTLRH--SQSTRESV-RPGST 2032
            |.:.:::     .|.....||.  ||....|| :||.|
Human   895 TMKQLEE-----LEEEFCRLRPLLSQLGGNSVPQPGCT 927

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	32/144 (22%)
		C1_1	1151..1202	CDD:278556	3/50 (6%)
		RhoGEF	1539..1731	CDD:238091	78/191 (41%)
		PH_RhoGEF	1770..1875	CDD:275411	32/112 (29%)
		DUF2413	2242..>2378	CDD:287302
ARHGEF1	NP_945353.1	RGS_p115RhoGEF	67..259	CDD:188709	49/247 (20%)
		Trypan_PARP	<330..425	CDD:114603	24/146 (16%)
		RhoGEF	435..619	CDD:214619	76/189 (40%)
		PH_p115RhoGEF	653..777	CDD:275429	38/141 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	138	1.000	Domainoid score	I4844
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	332	1.000	Inparanoid score	I2429
Isobase	1	0.950	-	0	Normalized mean entropy	S6267
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D163264at33208
OrthoFinder	1	1.000	-	-		FOG0001574
OrthoInspector	1	1.000	-	-		otm41901
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR45872
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3275
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	11	10.910