Protein Alignment RhoGEF2 and ARHGEF1

DIOPT Version :10

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: XP_047295618.1
• Gene: ARHGEF1 / 9138
• HGNCID: 681
• Length: 1041
• Species: Homo sapiens
• Alignment Length: 1308
• Identity: 310/1308 - (23%)
• Similarity: 494/1308 - (37%)
• Gaps: 431/1308 - (32%)

  Fly   851 DPNENN---RGAAAAGPGVFIESHQFTPMAGASSP--IPISLHSSHMHAAQSNDTQKEIISMEDE 910
            :|.|..   ||||:.||               |.|  :|:|                 ||..|||
Human    12 EPREMEDFARGAASPGP---------------SRPGLVPVS-----------------IIGAEDE 44

  Fly   911 N--SDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDM 973
            :  ::|:....::|..|.:|.::  ......|...|.:|....:|.|||..|..::......|:.
Human    45 DFENELETNSEEQNSQFQSLEQV--KRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEA 107

  Fly   974 RKWAYEIHSTFL----VPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLIS 1034
            :|...:.:.:||    |.|.|:     ..::|.|:|                    |.|| ||||
Human   108 KKAFLDFYHSFLEKTAVLRVPV-----PPNVAFELD--------------------RTRA-DLIS 146

  Fly  1035 E--------------------QLREFQQKRTAGLGTIYGPTDDKLAEAK--------TDKLREQI 1071
            |                    ||.:|:.||..|:    .|.:.:||:.:        :.:.||:.
Human   147 EDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGM----TPWEQELAQLEAWVGRDRASYEARERH 207

  Fly  1072 IDKYLMPNL----HALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRD 1132
            :.:.|:.:|    |.:..||.       :..|:.:|:.  :|               :.|...|.
Human   208 VAERLLMHLEEMQHTISTDEE-------KSAAVVNAIG--LY---------------MRHLGVRT 248

  Fly  1133 KS---------FKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNI 1188
            ||         |:.::||..|                                     :|.....
Human   249 KSGDKKSGRNFFRKKVMGNRR-------------------------------------SDEPAKT 276

  Fly  1189 HRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQ 1253
            .:..|.::|         |.|....:          |:..|.      .....|:|.|......:
Human   277 KKGLSSILD---------AARWNRGE----------PQVPDF------RHLKAEVDAEKPGATDR 316

  Fly  1254 ASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSV 1318
            ...|..||   .|.||     |....:|.|::...|        :.||.:...||...::|..|
Human   317 KGGVGMPS---RDRNI-----GAPGQDTPGVSLHPL--------SLDSPDREPGADAPLELGDS- 364

  Fly  1319 ASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNR 1383
               :|.       |..:...|...::.|:.|..||     :.|.....:.||||.  |:|:
Human   365 ---SPQ-------GPMSLESLAPPESTDEGAETER-----RWKRLSGRLGRSESL--RVSD---- 408

  Fly  1384 NSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVP 1448
              ||:.|..||.::                       ...||.......|:    |.:|..|..|
Human   409 --RRRPSRGSLGAK-----------------------GRGGGRSRSDVDMD----PSSATAVLGP 444

  Fly  1449 PMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDED 1513
                                 .::.:.:.|..|..|.|.                   ||.|.|:
Human   445 ---------------------ARRATPEPGDEGEPGRSG-------------------LELEPEE 469

  Fly  1514 DVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDH 1578
               ...|..:|..:.|.:|..::.||||:|:|:..||..|||.|::|..|||.|:.|......:.
Human   470 ---PPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEE 531

  Fly  1579 LLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQ 1643
            |..:| |:|..|.|:|..|...|.:||.|..:::..|||:|...|||..|....:.:::||:||.
Human   532 LQNIF-PSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQS 595

  Fly  1644 IALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPE 1708
            .|||.||.|:.||......::::||...||||:|||::||.:|||||||||.:::.:.|     |
Human   596 FALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNT-----E 655

  Fly  1709 NTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLD----RSSYDK--EEFKKLDLTQ 1767
            ..||.|.::.|.|..:.||..|||||:..||..:|::.||:||    |.|.|.  .|||.||:|:
Human   656 EPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITK 720

  Fly  1768 HHLIHDGNLT--IKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLH----TPLSITNKPVSPIMS 1826
            ..|:|:|.||  :.|:.:|::|.||.:::::||.:||::..||: |    ||.......:.|::.
Human   721 KKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKS-HSRTLTPTPDGKTMLRPVLR 784

  Fly  1827 IDADTLIRQEAADKNSFFLIKM--KTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSN 1889
            : ...:.|:.|.|..:|:::..  :.:|:.||.|.:.||.|.|....::.|.
Human   785 L-TSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAG------------- 835

  Fly  1890 HDTSISDPALAAIPH-----SNTKESLELSTDT------VQPLAATATLTTTPLAPMLPIATVTP 1943
               |:..||.|:.|.     |:|:|.|..|::.      ..|..|.   |...|:.:||..  .|
Human   836 ---SLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADAR---TERILSDLLPFC--RP 892

  Fly  1944 APATNNSNVSSLTGV------------------------QLRNPQRDATASESDADYVNTPKPRS 1984
            .|....:..:...||                        .||.|.|.|...:.
Human   893 GPEGQLAATALRKGVGGGILPPETPPVSAWGELCPPAWLHLRFPPRKAFCKKE------------ 945

  Fly  1985 SQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTR-----ESVRPGSTGEE-RNSTYGMV 2043
                       |:.||.::.:.            |..|.|     |.:|.||.|.. ..:..|::
Human   946 -----------RNGGEDVRDHP------------HPHSCRSISHPEGLRRGSCGPRLGGAQLGLL 987

  Fly  2044 GGNSKRDS 2051
            ..:..|.|
Human   988 APHEPRPS 995

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ_ARHGEF11-12-like	258..333	CDD:467282
		RGS_GEF_like	934..1055	CDD:188710	32/144 (22%)
		C1_ARHGEF-like	1150..1202	CDD:410382	3/51 (6%)
		RhoGEF	1539..1731	CDD:238091	78/191 (41%)
		PH_16	1756..1876	CDD:436083	39/129 (30%)
ARHGEF1	XP_047295618.1	RGS_p115RhoGEF	67..259	CDD:188709	49/247 (20%)
		RhoGEF	495..679	CDD:214619	76/189 (40%)
		PH_p115RhoGEF	713..837	CDD:275429	38/141 (27%)