DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF2 and TUS1

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_013529.3	Gene:	TUS1 / 851145	SGDID:	S000004417	Length:	1307	Species:	Saccharomyces cerevisiae

Alignment Length:	1060	Identity:	194/1060 - (18%)
Similarity:	310/1060 - (29%)	Gaps:	416/1060 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly   566 HRFKETGPTSKGKNKFL----ISRSLIEEDVPPPLPQRNPPRQLNLDLKNG--NASPGGSHLVAP 624
            :|:..:.|..:...|.:    ..|..||....|||..||   ....|..||  |.|.|.:    |
Yeast     2 YRYNRSSPFERTPEKRVSRQESQRKSIELPKLPPLNTRN---SFLDDSDNGTDNISIGWT----P 59

  Fly   625 VSDLDRATSPQLN----RSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASA 685
            :||..:..||...    .|:.....:.|.:|.|..||      ||.:.:.:
Yeast    60 ISDTQQFQSPVPQAFTFTSKHSARGNGTSSSESTPKS------TKYVKERR-------------- 104

  Fly   686 AGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNI 750
                         |||.||.|  ..||.:...|                |:||
Yeast   105 -------------PPPPPPLL--YSTESIRIDS----------------PMVS------------ 126

  Fly   751 AFPLSQ----RPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRV-------GSSPDN 804
              |.||    .||.:..:.  ..:::|.|.              ..:.|.|:       |.||::
Yeast   127 --PSSQSRERSPNKLSFIG--NSEERHHME--------------YISNHSRILKSPFANGFSPNS 173

  Fly   805 MHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIE 869
              |:.|...:|..:...:..:..........||.|:.:    |:|..|.::.            :
Yeast   174 --PKSPRDSSKQQAHFSDESDLRCHEREKALPPIPFTT----TLLLSPFDDE------------D 220

  Fly   870 SHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEA 934
            |..||     ..|.|:|...:    ...|....|  ::|...||.|..|.:.|...::...||.|
Yeast   221 SEFFT-----KPPPPLSTSRN----VSGNSRVSE--ALESVYSDSDYTFNNSNARQSSFNSLLGA 274

  Fly   935 ENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDES 999
            :                   ||                      |:..:...|..|.|....||.
Yeast   275 K-------------------PL----------------------ELAPSITAPTQPFSIQSIDEH 298

  Fly  1000 LAREVDNVLQLE------------------YDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTA 1046
            ...:.|||.:|.                  :.|:::.:.|.|             |.|||.....
Yeast   299 KLYQCDNVYKLSAIYEWILKVYFEWFNECVFTKIDLFQIVQL-------------LLEFQMPTNF 350

  Fly  1047 GLGTIYGPTDDKLAEAKTDK-LREQIID-----------------KYLMPNLHALIEDENGSPPE 1093
            ...||....|:.:|...:.| :|..||:                 ..|:| .::.|::..||...
Yeast   351 DQDTIDSNVDNIMASFISQKAVRFDIINDEEVAVVVGGLDITGVFTELLP-CYSFIDNTYGSTNS 414

  Fly  1094 DVRKVALCS-----------ALSTVIYRIFNT-----RPPPSSIVERVHHFVSRDKSFKSRIMGK 1142
            .:....:|:           .||.:|.:....     ...|..:.|.....|.|.......|:.:
Yeast   415 LICYSNVCTHGQSSGFRKEIKLSEIINKSVGLWTEYWHLTPDDLAEINPREVQRQSFIFDLIILE 479

  Fly  1143 NRKMNVRG-----------------------------HPLV-LRQYYEVTHCNHCQTIIWGVSPQ 1177
            .|.:|:..                             .||: |...:.:|      .|.|.:..:
Yeast   480 ERSLNMATAAVEIYGKRFDKSLLPDEPEFKALAFDIFEPLIQLHTEFLLT------PIFWKLKTR 538

  Fly  1178 GYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQD-- 1240
            |.....        ..|:..:.|    .:||.:..|  .:|.|..:.    ::|..||:::..
Yeast   539 GKFIDG--------VGKIYSKWC----GEAKNIYLN--YAKAMATVH----EIIMWEKKNKTKFV 585

  Fly  1241 ---EELD--VELTPDRGQASIV----------RQPSDRRPDANISIRSNGNTSC----------- 1279
               :|:|  ||:|..:....::          ..|...|     ||..|.:.|.
Yeast   586 TWLKEIDNSVEITRSKMYHDVIFFGGFFKSLQNMPVTLR-----SILKNTDPSMEDYEYLKIVIK 645

  Fly  1280 NTSGLN-------------------TTDLQSSFHGSCANDSINPGGGAGCNMD------------ 1313
            ....||                   :..|..|.:.|.|...:|.||....|.|
Yeast   646 EVEKLNFEVNQVHGLAIDHRKLVRFSKQLVLSTNSSNATSYVNVGGSTNANDDDAIQDKLALGLT 710

  Fly  1314 -------LSTSV-------ASTTPSTSGSVAAGLSAFAELN--------------ALDTVDKEAR 1350
                   ||.:|       ...||.....:...|....|::              .:|.:..|.|
Yeast   711 YPERKLVLSGTVYKKRDLWLDPTPVYIALLDNCLLITEEISKGETQKYKLIERPIPIDYLSLEKR 775

  Fly  1351 R---------ERYSQ----HPKHK-SAPVSVNRSESYKERLSNKRNRNSR----RKTSDPSLS-S 1396
            :         ..|||    .|.|. |.|::     |.:..|.:..|..|.    ||||:..:| :
Yeast   776 KIPGTSKQPLRNYSQKEHKSPMHNFSTPIN-----SMRPLLKSSGNHMSTAYGDRKTSNTEISNA 835

  Fly  1397 RPNDEQLDLGLSNATYVGSS 1416
            .||.::....:.| |..|.|
Yeast   836 NPNTDEFSFKIRN-TATGES 854

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	22/138 (16%)
		C1_1	1151..1202	CDD:278556	9/51 (18%)
		RhoGEF	1539..1731	CDD:238091
		PH_RhoGEF	1770..1875	CDD:275411
		DUF2413	2242..>2378	CDD:287302
TUS1	NP_013529.3	RhoGEF	469..655	CDD:238091	33/214 (15%)
TUS1	NP_013529.3	CNH	939..1287	CDD:214481
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900