DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and plekhg5b

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_017209177.1	Gene:	plekhg5b / 796404	ZFINID:	ZDB-GENE-090908-6	Length:	1110	Species:	Danio rerio

Alignment Length:	1406	Identity:	281/1406 - (19%)
Similarity:	489/1406 - (34%)	Gaps:	453/1406 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly  1107 VIYRIFNTRPPPS--SIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNH--C 1167
            :::...:..||.|  .|..|:|...|:|:           :..:..||...::.:....|:|  |
Zfish     7 IMHPALHKSPPRSWLGIRLRLHEKSSQDE-----------ECVLCQHPDCPKRRHASKVCHHPDC 60

  Fly  1168 QTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCP---------GPLPQAKRLAHNDKISKFMGKI 1223
            |.:. |.||. :.|..|....|      :|||..         ...||...||.|....    ..
Zfish    61 QELN-GKSPL-HLCESCDSRCH------LDESDSMHFDRHPRFDVQPQGSILARNVSTR----SC 113

  Fly  1224 RPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTD 1288
            .||||        ...|.|.|.|.:.:||:          |....:.:::...   |....:.||
Zfish   114 PPRTS--------PPSDLEEDEEASTERGE----------RKAGGLKLKAREG---NKPRRHHTD 157

  Fly  1289 LQSSFHGSCANDSINPGGGAGC-------NMDLSTSVA-----STTPSTSGSV--AAGLSAFA-- 1337
                          ||  ...|       |:|:.|.:.     .|.....|.:  ..|:..|.
Zfish   158 --------------NP--RRECFSLKFDLNIDIDTEIVPAVKKKTLREVLGPIFERKGIELFRVD 206

  Fly  1338 ----ELNALDTVDKEARR--ERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNS--------RRK 1388
                :.|...::|.||.|  .||.:........:.|.:|......||....:.|        ...
Zfish   207 LFLDQSNTPLSLDFEAFRFGGRYLKVKARPGDELKVEQSVKDLRSLSLPNIKPSVGGSVYILTPA 271

  Fly  1389 TSDPSLSSRPNDEQLD-LGLSNAT-----YVGS----SNSSLSSAGGSESPSTSMEHFAAPGAAG 1443
            |..|..||.|..:.:| ||.:..|     ::|.    |..:|:..||| .|:      ||||  |
Zfish   272 TDKPEHSSLPRRDSVDILGQARRTKDMKQFLGDASIPSMDTLALLGGS-LPT------AAPG--G 327

  Fly  1444 GVQVPPMGLNQNQHPH-----------------LLIQQHAQQYCQQDSFQAGLAGAAGSSAASNS 1491
            .:...|... :|:.|:                 .|:....|.|.     |.||.......|....
Zfish   328 SLTAGPNNC-KNRPPNRFSIFFGAGAEKEVDRVQLLNTKLQAYS-----QFGLPKLPPQLAFHQD 386

  Fly  1492 SFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRT 1556
            |            |..|.|:.:...|..|.:::  :...:|:..:.::||.|.|:.|||..:::.
Zfish   387 S------------WEEEEEEPNLTLEDSWRTLL--DDPESLSRRQFQQQEAIWELIQTEATYIKK 437

  Fly  1557 LKLLDRLFFLPLYESGLLS-QDHLLL--LFPPALLS-------------LREIHGAFEQSLKQRR 1605
            |:::..||.     .|||: ||:.||  :.|..|.|             ::.:..|.|::...|.
Zfish   438 LRVITDLFL-----CGLLNLQDNSLLGEVEPTRLFSNIQDIVRLHCTLWVQVMFPALEKARGSRS 497

  Fly  1606 IEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQF------CARQQIALEALKEKRNKDEMLQKLLK 1664
                         |.:..|.|.|  ...||::|      |..::..:|.::.....:|:.:..:.
Zfish   498 -------------LIEPTDLQEG--FSTFASRFQPYIRYCMEEESCMEYMRSLLRDNELFKIYIT 547

  Fly  1665 KSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVE 1729
            .:|::|.|.||:|.|:|....|||||||||.:.:.|.|     |:....:|:...|...:..:..
Zfish   548 WAETNKQCNRLKLADMLVKPHQRLTKYPLLLKTILKKT-----EDQASRDALNNMVACVEGFING 607

  Fly  1730 VNQAVKTAEDAHKLQNIQRKLDRSSYDK------------EEFKKLDLT------------QHHL 1770
            |:..::..::..||.....:::  ||:.            :||.::|||            |.||
Zfish   608 VDSQMRQRQEQQKLAATSARVE--SYEAVEGASEEVEKALKEFSRIDLTAPMIGTSPEETRQLHL 670

  Fly  1771 IHDGNLTIK--KNPSVQLHGLLFENMIVLLTKQDDKY-YLKNLHTPLSITNKPVSPIMSIDADTL 1832
              :|.|.:|  |:..:..:..||.::: |:||...:. .:|.:..||.:.|             :
Zfish   671 --EGALRMKEGKDSRMDAYCFLFTDLL-LITKPVKRLEKVKVVRQPLLLHN-------------V 719

  Fly  1833 IRQEAADKNSFFLIKMKTSQ----MLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTS 1893
            :.:|..|..||.||.:...:    ....:|.|:::.|:|.....:|..:..:.||:.|....
Zfish   720 VCRELKDPGSFVLIYLNEFKSAVAAYTFQANSAAQGKSWTDAICNVQNQLQRLRSEEARRQK--- 781

  Fly  1894 ISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGV 1958
                  |.:    .|..|:...|                           ..:.|::|.|.
Zfish   782 ------ACL----KKRLLDNQED---------------------------EDSCNSTNSSP---- 805

  Fly  1959 QLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQST 2023
            .||:  :|.:.|:||..              ..|:|:....|       :|... |.::.||.
Zfish   806 HLRH--KDQSNSQSDGS--------------TETLSVMDMDE-------SGLSL-DPSVLHSD-- 844

  Fly  2024 RESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTA 2088
                               :||:|..|||             :|.|:|.::|
Zfish   845 -------------------LGGHSDPDSA-------------ILQQNPELEP------------- 864

  Fly  2089 EPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIA---MMPEAPKDSADIALAA 2150
                    .|...|||                .|..::....:|.|   :.||:..::.:.|...
Zfish   865 --------HLNSQDAS----------------DLDPQYRSMSMDSAYGTLSPESMLETQNPAAPV 905

  Fly  2151 YDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGH---CHEKEKLRKKVAPSSSFSSSPPPLPPPNR 2212
            .::               .::|....||...|.   ..|::|:  .|...:|.:.|...|    |
Zfish   906 QEE---------------EKEEEDKESTETTGDEEGDDEEDKI--SVGSQNSITLSFNKL----R 949

  Fly  2213 QHAQAQAQIP-PSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKP-HERPTTPLAPFNT 2275
            :.|..|.::| ..||:.|.::.|.....:|:|              ||.|. ..|.|......:|
Zfish   950 RQAPVQRRLPFLERLVGKSRSEDNLLSTVHQD--------------IPKKKIKSRHTWEQRVKHT 1000

  Fly  2276 EPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEA 2340
            ||..|:|:.:....:|.:::|               |:.......::||..|.::....:...|.
Zfish  1001 EPALSRSLTELKAAESDESLP---------------TEESHRLTCSLPSAVLMDTLKRAKMARET 1050

  Fly  2341 DITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVA 2381
            . |.::.:.:.|..|.:.......:.:|..||.::...::|
Zfish  1051 G-TGQIPNSSASDGELSPPAGVEGTGNRRQKTQQHKKLTLA 1090

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710
		C1_1	1151..1202	CDD:278556	15/61 (25%)
		RhoGEF	1539..1731	CDD:238091	55/213 (26%)
		PH_RhoGEF	1770..1875	CDD:275411	24/111 (22%)
		DUF2413	2242..>2378	CDD:287302	24/136 (18%)
plekhg5b	XP_017209177.1	None
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910