DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and itsn2a

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_021324162.1	Gene:	itsn2a / 566412	ZFINID:	ZDB-GENE-041014-84	Length:	1666	Species:	Danio rerio

Alignment Length:	1875	Identity:	369/1875 - (19%)
Similarity:	631/1875 - (33%)	Gaps:	564/1875 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   230 LSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGD--NPVFVESVKPGGAAE 292
            ::||.:..:.||......     .|.|..|..|:     ||   :|||  ...|::|..|
Zfish     1 MNGGFSIWAITPEERGKH-----DKQFDSLAPTL-----GY---LSGDQARKFFLQSGLP----- 47

  Fly   293 IAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTRPSSVSVV---TPSTPIL 354
             ..::|....|...|.:.::::       ::.|..::|...:.|....|||:.::   || ||::
Zfish    48 -TSVLADIWALADIGKDGKMDR-------LEFSIAMKLIKLQLQGQPLPSSLPIIMKQTP-TPVM 103

  Fly   355 SGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKLR 419
            :...|..  .|..|                                        .||..::..:.
Zfish   104 TSSSRFG--MGSMP----------------------------------------NLSAMSVMPIL 126

  Fly   420 EQLHQVGAEDAPTVKLQA-----------------------AAGNKNTALLTPNQIQHLSASATH 461
            ..:        ||..:.|                       |..|.|..||||.....:|:..|.
Zfish   127 TPI--------PTCPVMAPLVPVPTLISGPLPQMPNSISVPALPNGNATLLTPTPAFPVSSGLTK 183

  Fly   462 SNQQFHHLHHHHNL-----HNNNYPPQQQPASTSPAFLS---LLPRSLSSLSLGTRKNKTEKDLT 518
            |          |:|     .::|.......||.||...|   .:|:| |.|......|..:|.::
Zfish   184 S----------HSLLDLGSSSSNSSSTTSLASNSPKTSSSDWAVPQS-SRLKYRQLFNSLDKLMS 237

  Fly   519 --TSSP---FGLTTDFLQQQRMSH-------------QAESMSQSMHQHTSTPTSQQF---FHPH 562
              .|.|   ..||...|.|.:::.             :||....:||......|.:..   ..|.
Zfish   238 GYLSGPQVRNALTASNLTQTQLATIWFLADVDRDGQLRAEEFILAMHLVEMAKTGRPLPLTLPPD 302

  Fly   563 QQQHRFKET-------GPTSKGKNKFLISRSLIEEDVPPPLPQRNPPR-----QLNLDLKNGNAS 615
            ......:.|       ||..      |||..|||    |.|||:|...     :|..:.:.|||.
Zfish   303 LVPPSLRTTKSCDLLNGPVP------LISTELIE----PELPQKNKSNVSYEDKLRENFQRGNAE 357

  Fly   616 PGGSHLVAPVS---DLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMF 677
            .....|.....   :.:|....:....:::|.....:......:::..:|:.:...:.:|..|..
Zfish   358 LEKRRLALQEQQRREEERRREEERREEEKRQQKEREERERREREAREMEIRRQREEERRMERQRE 422

  Fly   678 LQMESASAAGAAGGSIEVDGGPPPLPPRLPGM-MTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTT 741
            |:.:...                   .||..: ..|:..|...:...:...|      .|.:...
Zfish   423 LERQREE-------------------ERLKELERREEAERQRRREWEKSKRV------ELQTQKE 462

  Fly   742 AVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMH 806
            ..|||              :|:|:..::..:.::   :|.|          |||:::.|...:..
Zfish   463 KEQND--------------IQRLKDRKRSLEIEL---EAVG----------NKHKQISSQLRDAK 500

  Fly   807 PRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPN-------ENNRGAAAAGP 864
            .:.        .|.|..:|              .::......:.|.|       |..|.....||
Zfish   501 SKR--------QGQWAELE--------------LINQRRDVCITDINSLQLQFDEMQRQLNQLGP 543

  Fly   865 GVFIESHQ-------FTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPF---- 918
                |..:       ||  ...|||:             .||.::.:...:.....|.|..
Zfish   544 ----EKQRLADRLVHFT--QNISSPL-------------INDVKQSVCEKDRSCRKLKEQLDVLE 589

  Fly   919 ------IDENGPFNNLTRLLEAENVTFLAIF-LNYVISNSDPAPLLFYLITEL-YKEGTSKDMRK 975
                  :.:...:|...:.:|.::.....:. |...::|      ||.||.|| .|:...:|:.:
Zfish   590 RETTAKLSQMEQYNKEIKFVEMDDCVLQGLLSLLGCLTN------LFLLIKELRQKQSQQQDVLE 648

  Fly   976 WAYEIHSTFLVPRAPLSWYRQDESLAR-----EVDNVLQLEYDKVEILRTVFLRSRKRAKDLISE 1035
            ....:.|..|   ..|..:|::|...:     |.....|||.:::|  :....|.||.|::. ..
Zfish   649 QLRRVRSDKL---KELQRHRKEEEERKRKKEEEAARQAQLEQERLE--QEEAERQRKLAQER-EA 707

  Fly  1036 QLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQ-----IIDKYLMPNLHALIEDENGSPPEDV 1095
            :|||.||::  .|..:....:.  |.||.:|.|.:     .:|.:...:|.....:.|...|..:
Zfish   708 KLREEQQRQ--ALARLLEAQEQ--ARAKEEKRRAEEKERNELDVHTQSSLPFNSTENNILQPVVI 768

  Fly  1096 RKVALCSALSTVIYRI---FNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLVLRQ 1157
            .:|. ||:|:|  ||.   |..|......:| ...|:..|:|            .||....:...
Zfish   769 PEVT-CSSLTT--YRALYPFTARNSDELTLE-ADCFIQVDES------------TVRETGWLYGS 817

  Fly  1158 YYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDES--------------CPGPLPQAK 1208
            |    ..|.      |..|:.| ...|..:...|.:....||              ..||.|...
Zfish   818 Y----SGNR------GWFPESY-AERCSKDSQTQPTAAETESTAPSANYPGIPRVDIEGPTPTHT 871

  Fly  1209 RLAHNDKISKFMG--KIRPRTSDVIGNEKRSRQDEELDV----------ELTPDRGQASIVRQPS 1261
            .::.|.:.|:.:.  :...:|...:|..|    |:.:.|          ||...||..
Zfish   872 PVSTNTQHSQAVALCEWSAKTDSHLGFCK----DDIITVLEKQENWWYGELKEKRGWF------- 925

  Fly  1262 DRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDS------------INPGGG------- 1307
               |.:::|:.:..||       .:..|.||......:||            .:|..|
Zfish   926 ---PCSHVSVVTTNNT-------QSEPLYSSVEEIEVSDSGLLDEYVALYTYESPESGDLTFSAE 980

  Fly  1308 -------------AGCNMDL-----STSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERY 1354
                         .||..|.     |..|....|.|:.|...  |...|:..:.||......|:.
Zfish   981 DVVLVTEKEGEWWRGCIGDQTGLFPSNYVKPKEPDTASSAVP--SKKPEIAQVTTVSPATTPEQL 1043

  Fly  1355 SQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSS 1419
            |..|......:..|.:..:   |...:.|..:||..                     :..|||..
Zfish  1044 SLTPGQLIVVLHKNSNSWW---LGELQARGKKRKKG---------------------WFQSSNVR 1084

  Fly  1420 LSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQD--SFQAGLAGA 1482
            |..|.                 :|.:...|..|.|      :|..:..:...:|  |||.|....
Zfish  1085 LLEAN-----------------SGKITPAPQPLCQ------VIAMYDYKAANKDEMSFQKGQLIT 1126

  Fly  1483 AGSSAASNSSFWN---AG----HPLPVARWTLESEDEDDVNEADWSSMVAAEVLA--ALTDAEKK 1538
            ..:.  .|..:|.   ||    .|....:.|         .|.|.|....|::|:  ::...|:|
Zfish  1127 VLNK--DNPDWWKGEVAGVTGLFPTNYVKMT---------TECDPSQQWCADLLSLDSMCTEERK 1180

  Fly  1539 RQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQ 1603
            ||..|:|:.|||.:::..|:|...:|:.|:.|||.|::..:.::|    ::.||:.....:.||.
Zfish  1181 RQGYIHELIQTEESYLEDLELAVEVFYKPMAESGRLTEAEISMIF----VNWRELIMCSTKLLKA 1241

  Fly  1604 RRIEHNHV-----VNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLL 1663
            .|:.....     |..:||:|:.........:      :||:.|..|...|::|.:|....:..|
Zfish  1242 LRVRKKTAGERMPVQVVGDILSSELSHMQAYI------RFCSCQLNAAALLQQKTDKSLDFKLFL 1300

  Fly  1664 KKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEA----IQRAVESSK 1724
            ||..|:..|:.:.|...|...:||:|:||||.:|:.        |||..|.|    :|.|:|.::
Zfish  1301 KKIASNYRCKGMPLSSFLLKPMQRITRYPLLIKNIL--------ENTPPAHADHSNLQAALEQAE 1357

  Fly  1725 RILVEVNQAVKTAEDAHKLQNIQRK-LDRSSYDKEEFKKLD--LTQHHLIHDGNLTIKKNPSVQL 1786
            .:..:||:||:..|::.:|:.||.. |.....:...|..|.  |....|:|.|.|. |...|.:|
Zfish  1358 ELCSQVNEAVREKENSDRLEWIQNHVLCDGVIEHLVFNSLTNCLGPRKLLHSGKLH-KTKSSKEL 1421

  Fly  1787 HGLLFENMIVL------LTKQDDKYYLKNLHTPLSITNKPV---SPIMSIDADTLIRQEAADKNS 1842
            ..|||.:.::|      .:...||.:..|.:|...:...||   ..::.:.:|     .::|...
Zfish  1422 WALLFNDFLLLTYTSKQFSSSPDKLFNPNSNTQYKMYKTPVFLNEVLVKMPSD-----PSSDDPV 1481

  Fly  1843 FFLIKMKTSQMLELRAPSSSECKTWFK-------HFSDV---------AARQSKN 1881
            |.:..:  .::..|:..:.:|..||.:       ||.:.         .||..||
Zfish  1482 FHISHI--DRVYTLKTDTINERTTWVQKIKAASDHFIETEKLKREKAYQARSQKN 1534

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	14/75 (19%)
		RGS_GEF_like	934..1055	CDD:188710	30/127 (24%)
		C1_1	1151..1202	CDD:278556	9/64 (14%)
		RhoGEF	1539..1731	CDD:238091	55/200 (28%)
		PH_RhoGEF	1770..1875	CDD:275411	26/129 (20%)
		DUF2413	2242..>2378	CDD:287302
itsn2a	XP_021324162.1	EH	7..92	CDD:197477	23/110 (21%)
		EH	214..293	CDD:197477	17/79 (22%)
		SMC_N	453..>727	CDD:330553	64/361 (18%)
		SH3_Intersectin2_1	776..832	CDD:212921	17/81 (21%)
		SH3	881..932	CDD:327375	10/64 (16%)
		SH3_Intersectin_3	960..1011	CDD:212772	7/50 (14%)
		SH3_Intersectin2_4	1028..1086	CDD:212927	13/81 (16%)
		SH3	1101..1154	CDD:327375	12/60 (20%)
		RhoGEF	1181..1364	CDD:238091	55/200 (28%)
		PH_13	1383..1523	CDD:318790	30/147 (20%)
		C2_Intersectin	1524..1658	CDD:176021	4/11 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810