DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment RhoGEF2 and plekhg5a

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_009304320.1	Gene:	plekhg5a / 562861	ZFINID:	ZDB-GENE-060503-766	Length:	1157	Species:	Danio rerio

Alignment Length:	994	Identity:	214/994 - (21%)
Similarity:	369/994 - (37%)	Gaps:	271/994 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly  1347 KEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQLDL--GL-S 1408
            ::.|.||..:.|:..|.|:.         |.|:..:.:|..:....||..|   |..||  || .
Zfish   249 EDLRVERCVKDPRSLSLPIM---------RSSSNTHSSSTDRAEHGSLGRR---EAADLLVGLVG 301

  Fly  1409 NATYVGSSNSS----------LSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQ 1463
            :|.|:..:...          |..:..|...|.|....:.||:..|             |.....
Zfish   302 HAAYIQKNGGQTRRRKNMPEFLGDSSISNQDSLSQLSGSLPGSITG-------------PDRWRN 353

  Fly  1464 QHAQQYCQQDSFQAGLAGAAGSSA-------ASNSSFWNAGHP-----LPVARWTLESEDEDDVN 1516
            :.|.::....|..:| .|:.|...       :...|:...|.|     |...|.:.|.|...::.
Zfish   354 RAASRFSGLFSSNSG-NGSLGKECDRVEQLQSKLYSYTMFGLPKMPPQLSFHRDSWEEEPNLELE 417

  Fly  1517 EADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLF---FLPLYESGLLSQDH 1578
            |: |..::  |....||..:..:||.|.|:.|||.::::.|.::..||   .|.|.|||||::..
Zfish   418 ES-WKQLL--ENPEILTRRQCHQQEAIWELLQTESDYIKKLHVITDLFLCGLLNLQESGLLTEVE 479

  Fly  1579 LLLLFPPALLSLREIHG---------AFEQSLKQRRI----EHNHVVNTIGDLLADMFDGQSGVV 1630
            ...|| ..:..:.::|.         |.|::.::|.:    |.:...:|.|.......
Zfish   480 PTRLF-SNIQDIVQLHSSLWTEVMLPALEKARQKRTMLNPTELHQGFSTFGFRFKPYI------- 536

  Fly  1631 LCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLF 1695
                  ::|..::..:|.::.....:|:.:..:..:|:||.|.||:|.|:|....|||||||||.
Zfish   537 ------RYCMEEEGCMEYMRTLLKDNELFRIYVTWAETHKQCNRLKLTDMLVKPHQRLTKYPLLL 595

  Fly  1696 ENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK--- 1757
            :::.|.|     ::....|||.|.|.:.:..:..|:..::..|:..||..|..:::  :|:.
Zfish   596 KSVLKKT-----DDPPTREAINRMVAAVECFINSVDSQMRHREEKQKLAAIAGRIE--AYEAVEG 653

  Fly  1758 ---------EEFKKLDL----------TQHHLIHDGNLTIK--KNPSVQLHGLLFENMIVLLTKQ 1801
                     :|:...||          ....|:.:|.|.:|  |...:.::..||.: ::|:||.
Zfish   654 ASEEVERILKEYNNFDLMAPVMGAPPEETRQLLLEGALKMKEGKESRMDVYCFLFTD-VLLITKP 717

  Fly  1802 DDKY-YLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMK------TSQMLELRAP 1859
            ..:. .:|.:..||.|.|             ::.:|..|..||.||.:.      .|...:  |.
Zfish   718 VKRVEKVKVIRQPLVIHN-------------VVCRELKDPGSFLLIYLNEFGCAVASYCFQ--AN 767

  Fly  1860 SSSECKTWFKHFSDVAARQSKNRSKNASSNHD--------TSISDPALAAIPHSNT-KESLELST 1915
            |.::.::|.:...:...:..:.||:......|        :|:|..:..:|.|..| .:||.||
Zfish   768 SGAQGRSWIEAIYNAQNQLQRLRSETQRDESDEKDEDETESSMSTSSSPSIHHRETLSKSLSLS- 831

  Fly  1916 DTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDA------ 1974
                |..:..:..|..:|.|..:..|..:.....|..:||..        |.:.|.||:
Zfish   832 ----PSHSDGSTETLSVAAMDEVDEVVGSTVQTASMRNSLLS--------DDSFSTSDSKTLQAL 884

  Fly  1975 -DYVNT---PKPRS-SQNEVNRTMSIRS--------------------TGEPIQKYSANGTEAND 2014
             |.:.|   |:.|| |.:....|:|.:|                    .||..:.:..:..:.:|
Zfish   885 GDLIVTDLDPESRSLSIDSAYGTLSPQSMITELDLKGGVCQSEGEETDAGEEEEAFEDDEDDDDD 949

  Fly  2015 ----VTLRHSQST-RESVRPGSTGEE---RNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSP 2071
                :|...||.| .||....:..||   ||...||         |.:|....|   :||..:||
Zfish   950 GDDSITWNGSQITVNESYILDTNSEELNRRNDFSGM---------AQVVAGCRS---QTLRRRSP 1002

  Fly  2072 ------------------------LVDPTAIQ------VSISPAHTAEPVLTPGEKLRRLDASIR 2106
                                    |.||..::      ||.|..|.|:. .:..:::.:.|   .
Zfish  1003 VQPRPDYLQHFTLRSRSEDDLLQCLTDPLLLRPTGKNNVSKSLTHLAKQ-KSHSDEVFQTD---E 1063

  Fly  2107 NDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQ 2171
            ::|||..|.:                      :.|.|..:..|...|:|......|.    ..:.
Zfish  1064 DELLEDAKTL----------------------SSKLSNTLQRAEARQVQRTLSCPNG----QADS 1102

  Fly  2172 EVSAVSTAVCGHCHEKEKL 2190
            |.|...||..|...||:.|
Zfish  1103 EGSDAETAPPGGMEEKKDL 1121

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710
		C1_1	1151..1202	CDD:278556
		RhoGEF	1539..1731	CDD:238091	54/207 (26%)
		PH_RhoGEF	1770..1875	CDD:275411	25/113 (22%)
		DUF2413	2242..>2378	CDD:287302
plekhg5a	XP_009304320.1	UNC-79	<52..>126	CDD:291442
		RhoGEF	440..626	CDD:279015	52/204 (25%)
		PH_PLEKHG5_G6	683..781	CDD:270064	25/113 (22%)
		PH	685..779	CDD:278594	25/109 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810