DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and arhgef1b

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_021322373.1	Gene:	arhgef1b / 557983	ZFINID:	ZDB-GENE-060616-136	Length:	1082	Species:	Danio rerio

Alignment Length:	1350	Identity:	328/1350 - (24%)
Similarity:	537/1350 - (39%)	Gaps:	355/1350 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   858 GAAAAGPG------VFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSDLDE 916
            ||..||.|      .:.::|....::||....|:                ..||..|||:.:.||
Zfish     9 GARFAGVGRTCDSMDYEDAHNGRGLSGAQGLNPV----------------MNIIGAEDEDFENDE 57

  Fly   917 -PFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEI 980
             |.:|:.....|...||:: ..|.|.:||::|:...|.||:|.||..:|.|...:||.:|...|.
Zfish    58 QPMVDDQCIHFNSIELLKS-RPTHLLVFLHHVMLQFDCAPVLCYLHADLMKNLNTKDTKKHFGEF 121

  Fly   981 HSTFLVPRAPLSWYRQDESLAREVD----NVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQ 1041
            ::|||...|.|. .....::|.|:|    .::..|..|.......|.:|.:     |..||.:|:
Zfish   122 YNTFLEKGAILK-VSVPSNIAPEIDRLRPELINEEQQKRYANEIQFAQSPE-----ILRQLEDFR 180

  Fly  1042 QKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALST 1106
            |||..|:    .|.:.:|.:.::.:..::|                    |.|:::.|:..:|
Zfish   181 QKRMMGM----TPNERELNDVESHRPTDRI--------------------PMDMKEKAVAESL-- 219

  Fly  1107 VIYRIFNTRPPPSSIVERVHHFVSRDKS--------FKSRIMG------KNRKMNVRGHPLV--- 1154
             :.::....|   |||.      .:|||        :..:.:|      .|:|......|.|
Zfish   220 -LEKMVEANP---SIVP------DKDKSNSIFGAVAYYMKYLGVKTRAFDNKKSKSGWRPSVPSM 274

  Fly  1155 LRQYYEVTHCNHCQTIIWGVSPQ---------GYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRL 1210
            |:.              |.|:.|         ||.....:|:...:              |.|:.
Zfish   275 LKP--------------WSVNKQIKPVKPPGIGYDGKQSRLDFEAK--------------QNKQP 311

  Fly  1211 AHNDKISKFMGKIRPRTS-DVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSN 1274
            .|...:|..:.....:|. .|.|:...|...|||.:                      ::|:..:
Zfish   312 PHRVSVSDSVTPPGRKTGITVSGSTHGSESSEELPI----------------------SVSVTPS 354

  Fly  1275 GNTSCNTSGL--NTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFA 1337
            .:...:|.|:  |.||.:.:..|.    .::|                  .||.|:|    |...
Zfish   355 PDFQTDTVGIINNRTDPKPASEGG----EVSP------------------VSTGGTV----SICE 393

  Fly  1338 ELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPSLSSRPNDEQ 1402
            .|..:|...::.:       ...:..|.:|.||||    |..:| |:|||..|..:..||...:
Zfish   394 PLAVIDVPPEDIQ-------DSGRKKPRNVRRSES----LCVER-RHSRRGGSTRAKQSRSRSD- 445

  Fly  1403 LDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHL-LIQQHA 1466
            :||                       .|||..|..:|.....|.|     ..::.|.: |:....
Zfish   446 VDL-----------------------QSTSTSHPLSPSPQHSVSV-----EGDKTPEIALLTPTF 482

  Fly  1467 QQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAA 1531
            ....||:..:..|                           ||.| :|.:|   |..:.::|.|..
Zfish   483 VPAPQQEEIEPRL---------------------------LELE-QDPLN---WRELASSESLHK 516

  Fly  1532 LTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGA 1596
            |:..|.||||:|||::.||:.|||.|.:|..:|..||.:...::.:.:..:| .:|..:.|:|..
Zfish   517 LSKKEAKRQEVINELFVTEQAHVRMLSVLQTVFSRPLEKQEYMTANEVATIF-SSLDDIIEMHYV 580

  Fly  1597 FEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQK 1661
            |.::||:.|.|..::|.||...|.:.|.|..|....:..|:||:.|..|||.:|.::.||.....
Zfish   581 FYENLKKLREEDEYIVKTISTPLLNRFSGSEGEWFQKLTARFCSYQSWALEQIKSRQKKDPRFNA 645

  Fly  1662 LLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRI 1726
            .:.::||...||||:|||::|..:|||||||||.||:.|.|     |:.||.|.|.:|.||.::|
Zfish   646 FILEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNT-----EDATEKEKINQAAESCRKI 705

  Fly  1727 LVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK-----EEFKKLDLTQHHLIHDGNLT--IKKNPSV 1784
            |..||:.||..|:...|::.||:||.|....     .|:|.:|||...::.:|.||  :.|..::
Zfish   706 LNHVNEEVKQMENLLILKDYQRRLDTSGLKPSNELYSEYKTIDLTNRTMLFEGPLTWRVTKEKAI 770

  Fly  1785 QLHGLLFENMIVLLTKQDDKYYLK---NLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLI 1846
            .:|.:|..:::||..|||||..||   ..:..:....:.:|||:.::: ..:|:.|.|..:.::|
Zfish   771 DVHCVLLSDLLVLFQKQDDKMLLKCQSKSNIAVQEGKQMLSPIIKLES-VFLREVATDPKALYVI 834

  Fly  1847 KMKTS--QMLELRAPSSSECKTW---FKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIP--- 1903
            ....|  |:.||.|.|..|.|.|   .|...|...:.||:.:|...::...|:  |...|:|
Zfish   835 FTWDSGAQIYELVAQSVGERKNWTEAIKSAVDELKKASKSNTKLERNSRAGSV--PNNIAVPLYG 897

  Fly  1904 --H---SNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNP 1963
              |   |.|:...||..:.|...:.....:..||...|         |.|..::  ||
Zfish   898 PAHPPLSPTENGGELLKNRVGRFSEKIRESDPPLIDYL---------AANGFDL--LT------- 944

  Fly  1964 QRDATASESDADYVNTPKPRSSQNEVNRTMSIR-------STGEPIQKYSANG---TEANDVTLR 2018
                        :.:||..:|:...::..|.::       |..:..|....||   .|:.|
Zfish   945 ------------HSHTPPEKSATVALDEVMCLKRLLVGSISLSDDAQTLGENGGMAPESQD---- 993

  Fly  2019 HSQSTRESVRPGSTG-----EERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAI 2078
             :..|:.||...||.     ||........|...|..||.:|.|........:.:|       |:
Zfish   994 -ADGTQLSVEESSTEAGPEVEEHEEDVENKGEEEKGISAPLVLSEERMEEVQMRLQ-------AL 1050

  Fly  2079 QVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYD 2128
            :                |:|:|| ..:..|..:.|:.:.. :.|...||:
Zfish  1051 E----------------EQLKRL-RLVEEDHYKLQEALAK-YTLQGGHYN 1082

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	37/124 (30%)
		C1_1	1151..1202	CDD:278556	9/62 (15%)
		RhoGEF	1539..1731	CDD:238091	75/191 (39%)
		PH_RhoGEF	1770..1875	CDD:275411	33/114 (29%)
		DUF2413	2242..>2378	CDD:287302
arhgef1b	XP_021322373.1	RGS_p115RhoGEF	74..266	CDD:188709	55/234 (24%)
		RhoGEF	524..710	CDD:238091	75/191 (39%)
		PH_p115RhoGEF	744..868	CDD:275429	37/124 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580175
Domainoid	1	1.000	133	1.000	Domainoid score	I5011
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	361	1.000	Inparanoid score	I2160
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D163264at33208
OrthoFinder	1	1.000	-	-		FOG0001574
OrthoInspector	1	1.000	-	-		otm25705
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR45872
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3275
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.890