DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and Arhgef5

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_598435.1	Gene:	Arhgef5 / 54324	MGIID:	1858952	Length:	1581	Species:	Mus musculus

Alignment Length:	1972	Identity:	362/1972 - (18%)
Similarity:	634/1972 - (32%)	Gaps:	618/1972 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   204 QSDNSNPVLQAPGERSSLNLTPLSRDLSGGHTQESTTPATTPSTPSLALPKNFQYLTLTVRKDSN 268
            |.|.........|.|..:|.:.:.||: |.||..|                    :.:..:|:|:
Mouse    41 QEDRDPSYKWTDGHRPVMNQSKVLRDM-GDHTPNS--------------------MAIFFKKESS 84

  Fly   269 GYGMKVSGD------------NPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGL 321
              .|:.|.:            ....::|:|.    .::..:|...:|.....|..|:....:...
Mouse    85 --DMETSQEILLAEACNTPDQQEAVIQSLKD----RLSRTIAAPELLACAVQEEWLDIPSKLDNR 143

  Fly   322 IKA-------STTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMET 379
            :.|       |.|:.::.::.::.|.|.: |:...|.|                  ..|....||
Mouse   144 VGAELQSELMSLTLAVSKEKEEEETSPDT-SIPRGSWP------------------PCKTHPGET 189

  Fly   380 YKIQ-TLQKMLEQEK-LNLERLKSDQNNPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNK 442
            .:.| :..::|.|.| |.||..:.:|....: |.....:.|.||       :...|::| ..|..
Mouse   190 EQTQGSGSELLRQGKQLQLEATQENQGQEGF-LQSQEAQGLEEQ-------EGQEVEIQ-EEGTL 245

  Fly   443 NTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFL------SLLPRSL 501
            |..:.    ...|........:.|          |.|...|:|....|...|      ..|...|
Mouse   246 NEGIC----FGGLLGEQEEVEEGF----------NGNEEEQKQGQIQSYMLLGGQWENEGLSGEL 296

  Fly   502 SSLSLGTRKNKTEKD----LTTSSPFG------------LTTDFLQQQRMSHQAESMSQSMHQHT 550
            ..|:...|..:..:.    |..|...|            :.|.:.:.||:..::|.:.:....|.
Mouse   297 EGLNYSERGQENRERRVWVLRDSEEEGQDQESREVEERRVATQYTENQRLVEKSEIVKRKQRDHD 361

  Fly   551 STPTSQQFFHPHQQQHRFKETGPTSKGK----NKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKN 611
            .|....    |.:.|....::|...:|.    .:..:..|...||..|.||..:...::   |..
Mouse   362 QTGKVM----PVRDQKEVVDSGDRVQGNGDSGGQTAVEGSRPGEDSKPSLPVASVDPEV---LSP 419

  Fly   612 GNASPGGSHLVAPVSDLDR--------ATSPQLNRSQQQQLP----------SSTDNSPSNAKSK 658
            |...||.|..||.:..:.:        ..:|.|..::....|          .|.||...|::.:
Mouse   420 GTLFPGISSSVADIPQIQKEPVCEELSPQAPALEPTEWSHQPISPPASFAPEESLDNRTHNSQQE 484

  Fly   659 RSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPP-----------------LPPRL 706
            ..:::..            :::.|||.:.|..|:.:.....||                 ||...
Mouse   485 EFRLRKG------------IEVVSASTSVAPSGTRDSPPFSPPNVFSSTATLSPVSSSVILPEET 537

  Fly   707 PGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQ 771
            |.......:...|.....|.....:..||..::.||      |...|||....:.|.|:......
Mouse   538 PTASASADTPHHCGPCETPPLPAKSSRYPCATSDTA------NPHSPLSSYTGVTQHLRSNSFPG 596

  Fly   772 QHQMSGGQATGALGQTP-NLGKN---KHRRVGSSP------DNMHPRHPDR-----ITKTTSGSW 821
            .|:..         ||| :||.:   .|..:...|      .::.||...|     :...:|.:.
Mouse   597 SHRTE---------QTPDSLGMSLSFSHLELPQRPPKPAIYGSLTPRRNRRSRDGIVFSDSSTAL 652

  Fly   822 EIVEKDGE--SSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIP 884
            ..:::|.|  :|.|..|..|:.|.:.      .:..|...|...|            |..|||..
Mouse   653 FALKQDSEEFTSNPERPSSPHGSPTW------GSPQNSAFAIGSP------------ANVSSPPT 699

  Fly   885 ISLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVIS 949
            :|:..:         .::.::.:..|........::.:|..:.:...|:               .
Mouse   700 VSMDMT---------IREALLPIPPEKRHSYSHIVERDGLLHEVASTLK---------------R 740

  Fly   950 NSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDK 1014
            :|.|.||.  |.:.|::  :||.         |..|||         |.::||:
Mouse   741 HSHPPPLT--LSSGLHR--SSKG---------SFSLVP---------DSTVARQ----------- 772

  Fly  1015 VEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPN 1079
                                                 :.|                      :|:
Mouse   773 -------------------------------------HRP----------------------LPS 778

  Fly  1080 LHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNR 1144
            ..........|.|..:|               :|...||:..:...:| .|...|:.||:
Mouse   779 TPESPNHTQTSIPSRLR---------------YNKPLPPTPDMPEFYH-PSISSSYISRM----- 822

  Fly  1145 KMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESC-PGPLPQAK 1208
               .|..|.|                                       .::|.|. |.|||.
Mouse   823 ---YRPLPPV---------------------------------------PIIDPSSEPPPLPP-- 843

  Fly  1209 RLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRS 1273
                         |.|.|:..:.|....|             .|||    :|.....|...|..|
Mouse   844 -------------KSRGRSKSIQGGVIHS-------------GGQA----KPRPNNQDWTASTLS 878

  Fly  1274 NGNTSC-NTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTT---------PSTSGS 1328
            .|.||. ..:|.:|..|..:   |..|:.::| |.|..||....|.:|.|         |:|...
Mouse   879 VGRTSWPPATGRSTESLPLT---SRCNNEVSP-GLAFSNMTNLLSPSSPTTPWIPDLQRPTTKDE 939

  Fly  1329 VAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPS 1393
              :||:..:|.....:..:.|.:|.::.  ..:||..|...||........|.:....|:  ||:
Mouse   940 --SGLTEESEPPVRGSFRRSAPQEEFNN--TRRSALGSRKNSEKPLHHQLEKASSWPHRR--DPA 998

  Fly  1394 LSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAA---PGAAGGV----QVPPMG 1451
            .:|..:.||:.||.......|.:...|.........|:.:.:.||   ||::..|    :.|..|
Mouse   999 RTSESSSEQVVLGQVPNKQKGWNRQGLRRPSILPESSSDLRNPAAGRLPGSSDSVVFREKKPKEG 1063

  Fly  1452 LN----------------------------QNQ-------HPH-LLIQQHAQQ-YCQQDSFQAGL 1479
            :.                            |:|       .|| |...:|.:: ....||:...|
Mouse  1064 MGGFSRRCSKLISSQLLYQEYSDVVLNKEIQSQQRLDSLAEPHGLSSPRHRRKALVSSDSYLQRL 1128

  Fly  1480 AGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIIN 1544
                  |.||:.|.|   ..:||.|                    .:.||.::|..::|.||...
Mouse  1129 ------SMASSGSLW---QEIPVVR--------------------NSTVLLSMTHEDQKLQEAKF 1164

  Fly  1545 EIYQTERNHVRTLKL-LDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEH 1608
            |:..:|.:::|:|.: :|...........|.:|||..|.  ..|..:|::...|...|:: ..|:
Mouse  1165 ELIVSEASYLRSLNIAVDHFQHSAQLRGTLSNQDHQWLF--SRLQDVRDVSTTFLSDLEE-NFEN 1226

  Fly  1609 NHVVNTIGDLLADMFDGQSGVVLCEFAAQF-------CARQQIALEALKEKRNKDEMLQKLLKKS 1666
            |            :|..|...|:...||.|       ...|.......:...|.:...:::|:|.
Mouse  1227 N------------IFSFQVCDVVLNHAADFHRVYLPYVTNQTYQERTFQSLMNSNSSFREVLEKL 1279

  Fly  1667 ESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVN 1731
            ||...|:||.||..|....||:|:..||.:|:.|   |..|.::.|||| .:|..:.::::.:.|
Mouse  1280 ESDPICQRLSLKSFLILPFQRITRLKLLLQNILK---RTQPGSSEEAEA-TKAHHALEKLIRDCN 1340

  Fly  1732 QAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTI----------KKNPSVQL 1786
            ..|:......:|..:.:|::   ::.:.|..:..:: .|:..|.||.          :|..:..:
Mouse  1341 SNVQRMRRTEELIYLSQKIE---FECKIFPLISQSR-WLVKSGELTALEFSVSPGLRRKLTTRPV 1401

  Fly  1787 HGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTL-IRQEAADKNSFFLIKMKT 1850
            |..||.:.::|...::...:|...|.|.|          ||..:.. ::.....||.|.|..:..
Mouse  1402 HLHLFNDCLLLSRPREGSRFLVFDHAPFS----------SIRGEKCEMKLHGPHKNLFRLFLLHN 1456

  Fly  1851 SQMLEL----RAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESL 1911
            :|..::    |..:.||...|      ::|........:....:|:.......|..|..|.:.:|
Mouse  1457 AQGTQVEFLFRTETQSEKLRW------ISALAMPREELDLLECYDSPQVQCLRAYKPRENDELAL 1515

  Fly  1912 ELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADY 1976
            | ..|.|.                          .|..|:...|.||:|.:.:: ........::
Mouse  1516 E-KADVVM--------------------------VTQQSSDGWLEGVRLSDGEQ-GWFPVQQVEF 1552

  Fly  1977 VNTPKPRSSQN--EVNRTMSIR 1996
            ::.|:.| :||  |.:|..:.:
Mouse  1553 ISNPEVR-AQNLKEAHRVKTAK 1573

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	13/92 (14%)
		RGS_GEF_like	934..1055	CDD:188710	15/120 (13%)
		C1_1	1151..1202	CDD:278556	4/51 (8%)
		RhoGEF	1539..1731	CDD:238091	50/199 (25%)
		PH_RhoGEF	1770..1875	CDD:275411	24/119 (20%)
		DUF2413	2242..>2378	CDD:287302
Arhgef5	NP_598435.1	ARHGEF5_35	1..477	CDD:373847	91/511 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	25..54		2/12 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	159..274		29/156 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	316..643		66/360 (18%)
		PHA03247	<398..949	CDD:223021	141/815 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	659..700		13/58 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	741..810		23/175 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	829..1051		63/302 (21%)
		RhoGEF	1164..1340	CDD:366202	48/194 (25%)
		PH_ephexin	1362..1488	CDD:269929	26/142 (18%)
		SH3_ARHGEF5_19	1498..1552	CDD:212873	13/81 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810