Protein Alignment RhoGEF2 and arhgef18a

DIOPT Version :9

• Sequence 1: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Sequence 2: NP_999983.1
• Gene: arhgef18a / 407987
• ZFINID: ZDB-GENE-040516-14
• Length: 940
• Species: Danio rerio
• Alignment Length: 1106
• Identity: 246/1106 - (22%)
• Similarity: 409/1106 - (36%)
• Gaps: 294/1106 - (26%)

  Fly  1502 VARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFL 1566
            |.|..||| |..|.....||..|..:.|...:....|||::|:|:.|||.|||||||::..::..
Zfish    35 VLRDDLES-DARDFEAPTWSLAVDPQYLKNFSKDAVKRQDVIHELIQTEINHVRTLKIVLNVYIR 98

  Fly  1567 PLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIE-------HNHVVNTIGDLLADMFD 1624
            .|..:..:.:..|..|| |.:.:|.|:|..|..:||.||::       .|:.::.:||:|...|.
Zfish    99 ELRNTLQMDETRLERLF-PQVENLLEVHQFFLDNLKLRRLDSMEPGSSQNYCIHNLGDILITQFS 162

  Fly  1625 GQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLT 1689
            |:.|..|......||:|...|:...|:....::..|.|::|.......|||.:.:::..:.||:|
Zfish   163 GEIGSRLRYLYGMFCSRHTDAVNFYKDLMQNNKKFQNLIRKICQLSIVRRLGIPEIILLITQRIT 227

  Fly  1690 KYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSS 1754
            |||:|||.:.|.|    .:.:.|...:.:..:..|..:.:||..|..||...:|:.:..||:..|
Zfish   228 KYPVLFERVIKYT----EDESEEQMDLIKGPDFLKDTIAQVNAHVDEAEKEARLRELSSKLEPKS 288

  Fly  1755 YDK----EEFKKLDLTQ--HHLIHDGNLTIK---------KNPSVQLHGLLFENMIVLLTKQDDK 1804
            ..|    ..|::.|:.|  ..|:|:|.|..:         |:..|.:  ||..::::||.::|.:
Zfish   289 QVKMTSGRVFRREDMLQGKRKLLHEGMLNWRVTNNKGKGDKHTDVIV--LLLSDVLLLLLEKDSR 351

  Fly  1805 YYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLI--KMKTSQMLELRAPSSSECKTW 1867
            .      |..|:..||.  ::|: ...::|:.|.::.:.|||  .:.|.:|.|....|:.|..||
Zfish   352 L------TFASLDGKPA--VISL-LKLIVREAAHNEKAMFLISASLDTPEMYEFHTSSAEERNTW 407

  Fly  1868 FKHF-------SDVAARQSKNRSK------NASSNHDTSISD-------------PALAAIPHSN 1906
            ....       .||...:.:..||      .....||.||..             .::..:..::
Zfish   408 TNKIWKAVECCPDVKEEEPEELSKKIWEFQRKLCVHDASIEQHLTEKLKLFSSIAASVTGVSDTS 472

  Fly  1907 TKESLELSTDTVQPLAATATLT-----TTPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRD 1966
            |.|...|..:..:||.....||     ...|..:|.:....|.|.|     |..|.:.|:.|..
Zfish   473 TSERHPLRGNAAEPLQGERLLTGALKDVENLQNLLVMCEQGPTPFT-----SRATDLVLKKPHA- 531

  Fly  1967 ATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEAN---DVTLR--HSQSTRES 2026
            .|..|::...:    |.:....     |:.|.|:.||       .||   |..||  |....:|
Zfish   532 VTGLENNTHML----PENGFES-----SMESMGDRIQ-------VANTDLDPQLRELHLSENQE- 579

  Fly  2027 VRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPV 2091
                .:.:|..|.:     ..|.:..|.|    |..:|.|.....:|......:.:..| |.|.:
Zfish   580 ----LSADENLSVH-----FPKAEFYSTV----SQLSRKLYSLQSVVQQLESHIKVQHA-TIEEL 630

  Fly  2092 --------LTPGEKLRRLDAS---------IRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEA 2139
                    |...||.|.|...         ::|..|::|:           .::|..:..|:...
Zfish   631 KSRPRGNSLLEQEKHRNLHKQNEELANFQRLQNQRLQEQR-----------QWEQERERQMLQAE 684

  Fly  2140 PKD----SADIALAAYDQI-----QTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEK--LRKK 2193
            .::    ..::|.:..:::     |.|.:...||     :|::..:..::.....||||  |:||
Zfish   685 AREKELREREMACSRQEEMLAGEKQELARCREEY-----QQDLERLRESMRSVEKEKEKLELQKK 744

  Fly  2194 VAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAI 2258
            ...:.:.   |...|..|.     |.||||.  .|.|..:          ..|:.|    |.|.:
Zfish   745 YKKNENV---PVVYPLENE-----QIQIPPP--YPILNIV----------SPGFTE----RAPLV 785

  Fly  2259 PSKPHERPTT-----PLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNE 2318
            |  |.....|     |..|.:....|:|::...|.:|.   :|..|..|.  :|.:..:|..::.
Zfish   786 P--PRRESITGSLVKPEVPIHLISTTNQTLKAGSVQQK---IPTKLAAQP--KGKEKHSKHRNSH 843

  Fly  2319 VKTVPSDKLSESCNEERQCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADK 2383
            .:|                                |.||.           |:.:......||.:
Zfish   844 QRT--------------------------------NSAAG-----------IEVSHVVPIKVAGR 865

  Fly  2384 KEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSI 2448
                    |.|....:.||       ||....           |:...|...|..||..|:.:|
Zfish   866 --------EGGSLKAIRSS-------SPHHLH-----------PDLFTHPDKLSSSVSSHSGNS- 903

  Fly  2449 VTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVE 2513
                                .|:.....|.|.....|. |...|  :.|::|       :|||:.
Zfish   904 --------------------SRKHNHSHNSHTSSTHST-HSGHR--KTKDSP-------NAEDIF 938

  Fly  2514 F 2514
            |
Zfish   939 F 939

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710
		C1_1	1151..1202	CDD:278556
		RhoGEF	1539..1731	CDD:238091	59/198 (30%)
		PH_RhoGEF	1770..1875	CDD:275411	29/122 (24%)
		DUF2413	2242..>2378	CDD:287302	23/140 (16%)
arhgef18a	NP_999983.1	RhoGEF	74..266	CDD:279015	58/196 (30%)
		PH-like	307..427	CDD:302622	30/130 (23%)
		DUF4200	650..747	CDD:290574	18/112 (16%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810