DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and Y105E8A.25

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_001300405.1	Gene:	Y105E8A.25 / 3564893	WormBaseID:	WBGene00013682	Length:	1619	Species:	Caenorhabditis elegans

Alignment Length:	1895	Identity:	356/1895 - (18%)
Similarity:	625/1895 - (32%)	Gaps:	570/1895 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   589 EEDVP--PPLPQRNPPR-----QLNLDLKNGNASP--GGSHLVAPVS----DLDRATSPQL---- 636
            ||:..  ||||...||.     ::...|.|.::||  ..::||...|    .....|||.:
Worm     5 EEEASSLPPLPSSPPPEPVDPTRITFTLSNESSSPRYEFNNLVTSSSPSYFSYQPYTSPGIYLLP 69

  Fly   637 NRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPP 701
            :|.||....:.                              .|.||:           :....|.
Worm    70 DREQQPYFGAD------------------------------FQTESS-----------ISWNSPT 93

  Fly   702 LPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQ 766
            .||..|.::.|:|..|.          ||                    |.|::.| :|...|..
Worm    94 SPPPPPMIVEEEMRSGG----------GT--------------------ARPVTPR-SIANSLDS 127

  Fly   767 YQQQQQHQMSGGQATGALGQTPNLGKNKHRRVG---------SSPDNMHPRHPDRITKTTSGSWE 822
            ..:..||.........|     |...::.|.:|         .:.|::.....|.:..||:
Worm   128 LSECAQHHHHHSDVVNA-----NSNSDRSRSLGDLLAATMQDGNDDSLRNGSYDTLVTTTT---- 183

  Fly   823 IVEKDGESSPPGTPPPPYL--SSSHMTVLED------PNENNRGAAAAGPGVF----IESHQFTP 875
               |.|..:..|......|  ....|.:::|      |:.:......:.|..|    :....:.|
Worm   184 ---KGGGLTDSGIGGGLLLMKGEDEMDIMDDHNSSSSPDHHRIPTDISSPDSFTYPPVSFTSYDP 245

  Fly   876 MAGASSPIPISLHSSHMHAAQSNDTQKEIIS-----MEDENSDLDEPFIDENG---------PFN 926
            :.|:.| :|          :.||:....:.|     ..|:....|.|.|.|..         |:|
Worm   246 VNGSGS-VP----------SYSNENTNPLDSTPRGHSADDRKHSDLPAISEESEHEQQSIHIPYN 299

  Fly   927 N------------LTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYE 979
            :            ...:.|....|:     ..|..||.|:..:|......::...|.|:.:
Worm   300 HYPPHSSSVGPGAAPAVREVVEHTY-----PMVHCNSAPSNTVFNSDRIAHRRVVSDDVTE---- 355

  Fly   980 IHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKR 1044
              :|.|         ::.|.| ..:|.|.::.::|.........:|||.|..:......|..:
Worm   356 --TTRL---------KRSEEL-ENMDKVGRISFEKRSASTNHLSKSRKGATLVFHNDPNEKDE-- 406

  Fly  1045 TAGLGTIYGPTDDKLAEAKTD------KLREQII-------DKYLMPNLHALIEDENGSPPEDVR 1096
                      |||:  |...|      |..|.:|       ::|.|    |.::.:||||   :|
Worm   407 ----------TDDE--EEYEDAADGYSKDNEMLILEKSGGEERYRM----ASVKKKNGSP---LR 452

  Fly  1097 KVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRK-----MNVRGHPLVLR 1156
            .....|.|::    |.:|.|||..:..||....|..:|...|...::.|     ..:.||.:
Worm   453 HFDNHSNLTS----ISSTEPPPVRLPRRVSAVSSTSRSSPQRATDQSWKDKMPPFGIVGHSM--- 510

  Fly  1157 QYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMG 1221
                             .|||.....|.                   :|:          :.|.|
Worm   511 -----------------YSPQSLPSRDA-------------------IPR----------TPFDG 529

  Fly  1222 K-IRPRTSDVIGNEKRSRQDEELDVELT-PDRGQASIVRQPSDRRPDANISIRS----NGNTSCN 1280
            . |....|..||..:|     :...|:| ||          .|..||:..|..|    |.:.|..
Worm   530 STISSGASSNIGGSQR-----QFSHEVTSPD----------GDETPDSRYSTSSRLYENHSISSP 579

  Fly  1281 TSGL--NTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAFAELNALD 1343
            |:.|  ..||........|.|..::      .:..::|..:.:.|..|.|..|..|:|..    .
Worm   580 TALLAAELTDDLMPLSEICKNHRLS------ISSSINTEHSQSPPPKSQSGGAPNSSFVR----R 634

  Fly  1344 TVDKEARRERYSQHP------------KHKSAPVSVNRSESYKERL-----SNKRNR---NSRRK 1388
            ::.|..:|...|...            |.:::.||.:...|.:|||     |.|:.|   |::::
Worm   635 SLRKIMKRGSVSTSALGGDEGLRNGSLKRRASNVSSSFFASAQERLRPRAKSLKKQRIGKNAKKE 699

  Fly  1389 TSDPSL------------SSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGA 1441
            .|....            :||||...:...|...:..|.......|||.:...|||........|
Worm   700 KSGSDTFTFGNLFRKKPPTSRPNLADIISDLDQRSTTGGRGGGSLSAGATNRRSTSATILPMSSA 764

  Fly  1442 AGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSS----AASNSSFWNAGHPLP- 1501
            .|.....|.                ...|..|.....::.....|    .|::||..|..:.:.
Worm   765 GGCDPTTPF----------------DSACSLDGLPDHISRMVSPSDSPHHATSSSTLNGANSMSS 813

  Fly  1502 --------VARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLK 1558
                    ..:..|.:.||    ...|........|:.   .:.|:|:.|.|:|..|:.|...:.
Worm   814 LYVQLDRIYGKHDLFANDE----YQSWHEKYRHNTLST---KQVKKQDAIFELYLMEKRHCANIA 871

  Fly  1559 LLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQR---RIEHNHVVNTIGDLLA 1620
            .|...:...:.:..::::..:.:|.|..|..|.    .|..::.:|   |::.|:.|.||.|:::
Worm   872 FLLHGYRRRMLDENIITKQDMDVLIPDVLEPLL----IFHLNVLERITARMKENYEVGTISDIIS 932

  Fly  1621 D---MFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKA-CRRLELKDLL 1681
            :   :..||...:.|:....|...::.:........||:....:..||:.|.:. .:..:.:.|:
Worm   933 EELAIDGGQHTKLCCDAYTDFGVAKERSDVLYHHLMNKNAKFAEFFKKTYSEETFYKHYDFRPLI 997

  Fly  1682 PTVLQRLTKYPLLFENLYKVTVRLLPEN----TTEAEAIQRAVESSKRILVEVNQAVKTAEDAHK 1742
            ..::.|.|||.||.|.:.|        |    :...:..:.|:|::::...:::..:..|..:.|
Worm   998 TKIIGRATKYSLLLETILK--------NEQPFSEYHDLTKTALETARKFAHKIDGNLSMAHMSMK 1054

  Fly  1743 LQNIQRKLDRSS-------------------YDKEEFKKLDLTQHHLIHDGNLTIK--KNP---- 1782
            .:.::.::|.||                   :|.:...  ..|...|:|.|...||  .||
Worm  1055 WEELKAQIDSSSATTLYVSDPSVANATIRYTFDLDSLN--SATNRKLVHLGEALIKIPVNPVGSG 1117

  Fly  1783 --------------SVQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLI 1833
                          ..|.:.:||:::||:|.::..:..       :.:.::.|.|:.|:    ||
Worm  1118 GGTHSSSSGKQVDKKDQCYIVLFDDIIVILIRKSSRQL-------IFMQDQGVMPVQSL----LI 1171

  Fly  1834 RQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPA 1898
            | .||..:|..||......:.|:...:|::.|.|.. ..::|.:.........::.....:....
Worm  1172 R-TAARGHSIMLISGAKPVLFEISFHTSTDRKKWVT-LLELAPKNVPPEGIRITTGDADGVLRDR 1234

  Fly  1899 LAAIPHSNTK--ESLELSTDTVQP-LAATATLTTTPL---------APMLPIATVTPAPATNNSN 1951
            :||...:..|  :.||...||..| ..|.|....|.|         ...||..|.|.   .:|..
Worm  1235 VAAQQDAEDKWLKELEAIFDTRLPEEEALAKYLETRLEFFDNVREHVSNLPFKTRTD---ISNRI 1296

  Fly  1952 VSSLTG----------------VQLRNPQRDA-----------------TASESDADYVNTPKPR 1983
            ..::.|                |:.....|||                 .:|:...|..|:....
Worm  1297 REAVKGRFRELRRARVIPLNRLVERITESRDADLWSYFDEKADAADVLEKSSDLSEDSDNSGDSA 1361

  Fly  1984 SSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSK 2048
            :...:..|..:...|.:  |..|:||..|.:..|    |....:|..:|....||.    ||:..
Worm  1362 ARGAKPRRIQTFHGTSQ--QPGSSNGGGAIESIL----SENNGIRRHTTVPRMNSD----GGSGS 1416

  Fly  2049 RDSASIVCSNNSNNTRTLLMQSPLVDPTAI---QVSISPAHTAE-------PVLTPGEKLRRLDA 2103
            ..||:....:::.:      :.|:.|..|.   .:|.|...:|.       ...|.||.|.  |.
Worm  1417 SSSAAAAAGSSTTD------RIPIPDDVAFDEENISASAQGSARGRGDSELDRKTYGEMLS--DL 1473

  Fly  2104 SIRNDLLEKQ---KIICDIFRLPVEHY----------------DQI-------------VD--IA 2134
            .||..:..::   .:|.::..|..|::                :::             ||  :.
Worm  1474 PIRQTMRARRASTNLIKEVISLRKENHLLKNDNALTKSRCALLERVRGGGAMMTSTATAVDDSME 1538

  Fly  2135 MMPEAPKDSADI---ALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAP 2196
            |:.:..|:..::   .||..|::....|.::     ..|:|:.....|:.....|:::....|..
Worm  1539 MLRKKEKEIRELQQKVLADRDELAEKQKAID-----AQEEEIQTKWAALQARSIEQQQSPMHVRS 1598

  Fly  2197 SSSFS--SSP 2204
            |||.|  |||
Worm  1599 SSSHSQVSSP 1608

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	20/120 (17%)
		C1_1	1151..1202	CDD:278556	5/50 (10%)
		RhoGEF	1539..1731	CDD:238091	40/202 (20%)
		PH_RhoGEF	1770..1875	CDD:275411	28/124 (23%)
		DUF2413	2242..>2378	CDD:287302
Y105E8A.25	NP_001300405.1	RhoGEF	852..1043	CDD:238091	40/202 (20%)
		PH_16	1095..1211	CDD:375371	29/128 (23%)
		OmpH	<1540..>1591	CDD:214922	9/55 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810