DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and cyst

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_001260588.1	Gene:	cyst / 35237	FlyBaseID:	FBgn0032796	Length:	1319	Species:	Drosophila melanogaster

Alignment Length:	1512	Identity:	299/1512 - (19%)
Similarity:	506/1512 - (33%)	Gaps:	481/1512 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly  1116 PPPSSIVERVHHFVSRDKSFKSRIMGKNR---KMNVRGH-PLVLRQY---YEVTHC---NHCQTI 1170
            |||:|             :..|..:..|.   .:||..| |.:..:|   :|.|..   |..:|:
  Fly    64 PPPAS-------------NPASNTINNNNMIPTINVTPHSPALASKYNNIFEDTLSQLQNIRETV 115

  Fly  1171 IW--GVSPQGYHCTDCKLNIHRQCS-KVVDESCP-------GP-------------LPQAKRLAH 1212
            :.  ..|....|..|..|..|...| .::..|.|       ||             .||..:..|
  Fly   116 VQMKNSSSPSQHMQDGSLANHGLLSAAILSTSLPDLTGTGTGPASGGSNYAMWSSTAPQQCQFLH 180

  Fly  1213 NDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTP-DRGQASIVRQPSDRRPDANISIRSNGN 1276
            .|         |.::...:.:...:.......|.|:. |..:...:|....||..|.     |..
  Fly   181 TD---------RRKSWTAVDDGSGAGDCTNKSVSLSSLDSEEQETIRVTEQRRRSAR-----NST 231

  Fly  1277 TSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTP-----STSGSVA------ 1330
            ...:|..||..:|...|....|..|            |:|.:.|..|     |||..:|
  Fly   232 GGISTHSLNEAELARDFERIAAKRS------------LATEIISRIPLQKSISTSSIIAKEDIKA 284

  Fly  1331 -------------AGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRN 1382
                         :.|.|.|::...|||:|         .||..|...               |.
  Fly   285 IARHLTDSEDENQSLLRAHAKIEVYDTVEK---------RPKRGSIFF---------------RK 325

  Fly  1383 RNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQV 1447
            :..:.||.                    :.||...|:....|.:.:.:...||...      .:|
  Fly   326 KKPKAKTK--------------------SLVGGQLSACDVCGAAITLAQIKEHHLE------CKV 364

  Fly  1448 PPMGLNQ------------NQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPL 1500
            ..||..|            |.....||:... |:..:...:|...||.
  Fly   365 KKMGGGQDYYDGPDASTFSNPEDQPLIRSDL-QFLNEAPIEAQDLGAD----------------- 411

  Fly  1501 PVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFF 1565
            ||....|:..|       .|:..|..|:|..|.|.:.||||.|.|...||::|.:||.::.::|.
  Fly   412 PVLGIVLKEHD-------SWTPNVPRELLKTLKDLQIKRQEHIYEFIMTEKHHCQTLIVMQKVFV 469

  Fly  1566 LPLYESGLLSQDHLLLLFP--------PALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADM 1622
            ..|       :.|    ||        |.|..|.::|..|.:.|:.::.| .|:|::|.|:|.|.
  Fly   470 ESL-------ERH----FPSLNLNSMFPRLQQLTDLHTCFLRQLRLKQRE-QHMVDSIADILLDF 522

  Fly  1623 FDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKAC------RRLELKDLL 1681
            |..:....|.....:|||..:.||:..|....:...       :|.:|.|      ::..:.:.:
  Fly   523 FSLEQAQCLRLAYGEFCANHRSALDQFKLCLTEPSF-------AEWYKHCLQNPLLKKKGIPECI 580

  Fly  1682 PTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNI 1746
            ..|.|||||||||.|.|.| :.|   :|..|.:.:|.|:...|.:||:|:..|...|...:...|
  Fly   581 LFVTQRLTKYPLLIEPLLK-SAR---DNKLETDKLQHALNLVKSLLVDVDACVAEKELRERQLEI 641

  Fly  1747 QRKLDRSS---YDKEEFKKLDL---TQHHLIHDGNLTIKKNPSVQLHGLLFENMIVLLTKQDDKY 1805
            ..::|..|   |..:.|:|.:|   ::..|..||..|:       :.|.....:::::...|...
  Fly   642 YGRVDAKSFAIYKNKPFRKTELGVESRRRLKFDGLATL-------MQGRAKTQLVLVVVLTDCLC 699

  Fly  1806 YLK-----NLHTPLSITNKP-VSPIMSIDADTLIRQEA-ADKNSFFLIKMKTS--QMLELRAPSS 1861
            :|.     |.::..:..:|. |.|:..:    |||::| .:....::|.....  :|.||:..:.
  Fly   700 FLSENSGHNKYSFFTPEHKAGVVPLQKL----LIREKAGTESRGIYIISSNPDFPEMYELKVQTP 760

  Fly  1862 SECKTWFKHFSDVAARQS--KNRSKNASSNHDTSISDPALAAIPHSNTKESLE--LSTDTVQPLA 1922
            .:..||.:     ..||:  ...:.:.....|.:..:.....:   |.:|::|  ...|..|.|.
  Fly   761 KDKNTWIQ-----TIRQAVLDCPATDIIEAEDLTAEEKLRIGV---NKRETIEKMRQKDIEQALL 817

  Fly  1923 ATATLTT--TPLAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDA-----DYVNTP 1980
            ....|..  ..|....|...|    |..||:.|:...:......:|..:::.|.     ..:||.
  Fly   818 LEEKLMLQFNLLKEQQPFGDV----AGTNSSCSAANFLAAFGSYKDLVSADCDTAELWRRVLNTV 878

  Fly  1981 KPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYG---- 2041
            :..|   ::..|:...:||:|:.:           ||            .|.||:::..|.
  Fly   879 QDIS---QLASTIYSAATGQPVPR-----------TL------------SSVGEKQSEQYASPTL 917

  Fly  2042 ------MVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRR 2100
                  ..|.:.||...:                                  ::.||||  :|:.
  Fly   918 PKRAETFAGFDEKRGKLA----------------------------------SKLVLTP--RLQE 946

  Fly  2101 LD---------ASIRNDLLEK-----------QKIICDIFRLPVEHYDQIVDIAMMPEAPKDSAD 2145
            |:         :|:....||.           |.::|.|.:......:..:.:|:..|:|:.|
  Fly   947 LEEKREPKSGSSSVSEQPLENNYAVLQVTHHLQTLLCVISQQMTTINNLQLQLALYRESPRSS-- 1009

  Fly  2146 IALAAYDQIQTLTKMLNEY-----------------------MHVTPEQEVSAVSTAVCGHCHEK 2187
             |....||::.|..:.::.                       ..:..::::.|....|   ..::
  Fly  1010 -AYTHKDQLEELRNLQDKQQEEKTAWQRQLKQQQEELAEMRAQQLQLQKQIKAEQEDV---RQQR 1070

  Fly  2188 EKLRKKVAPSSS---FSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGY-- 2247
            |:|.||:...||   ..|...||||.:..|.      ||:.|        ||:  .||.|:|.
  Fly  1071 EQLYKKMELLSSQGLLLSPSTPLPPVSNNHP------PPAAL--------LDD--SHETDNGLSG 1119

  Fly  2248 ---------------------------CEIDELRLPAIPSKPHERPT-----TPLAPFN-TEPKT 2279
                                       |:...:.|.:..:.|...||     .|:...: :...|
  Fly  1120 SGTGSSTPSVGGTFDRRKEKWLSGSSSCKTPPVNLISANNAPKVNPTLVKQKLPMKLSSLSSSGT 1184

  Fly  2280 SQSVIDAS----KRQSTDAVPEGLLEQE-PLEGDKTETKGEDNEVKTVPSDKL----SESCNEER 2335
            |.|.:|.|    ....|..:..|.::|. ||:.....|....| ..|.|:..:    |.:.:...
  Fly  1185 SSSRVDKSASFNSASPTPYISAGSVQQMFPLKLADRRTATPSN-CTTTPTTGMVPQHSRTGSSPA 1248

  Fly  2336 QCVEADITKEVADPTTSKNEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVAST-- 2398
            ...:|..|........::.|:.|.....|:.||                        .|:|.|
  Fly  1249 IIQQATTTATPTQTPVARAESVAHYTRTPTSSR------------------------TGIARTAG 1289

  Fly  2399 VDSSTQTSPTESPKETD 2415
            :..:|.|:|...|:|.:
  Fly  1290 IAVATGTAPRAKPEEEE 1306

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710
		C1_1	1151..1202	CDD:278556	15/67 (22%)
		RhoGEF	1539..1731	CDD:238091	60/205 (29%)
		PH_RhoGEF	1770..1875	CDD:275411	21/113 (19%)
		DUF2413	2242..>2378	CDD:287302	31/179 (17%)
cyst	NP_001260588.1	RhoGEF	447..627	CDD:279015	58/202 (29%)
		PH_ARHGEF2_18_like	671..774	CDD:275432	23/118 (19%)
		PH	681..774	CDD:278594	19/101 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45448306
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm9245
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	4	3.740