DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and Itsn2

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_038969030.1	Gene:	Itsn2 / 313934	RGDID:	1590890	Length:	1716	Species:	Rattus norvegicus

Alignment Length:	1710	Identity:	321/1710 - (18%)
Similarity:	565/1710 - (33%)	Gaps:	497/1710 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   481 PPQQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQS 545
            ||...||...|:.      |.|||..||                                   .|
  Rat   151 PPLMMPAPLVPSV------STSSLPNGT-----------------------------------AS 174

  Fly   546 MHQHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLK 610
            :.|..|.|.|.... ||...:.....|.......|   ::|||                   ||.
  Rat   175 LIQPLSIPYSSSTL-PHASSYSLMMGGFGGASIQK---AQSLI-------------------DLG 216

  Fly   611 NGNASPGGSHLV--APVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKS---KRSKIKTKALSDP 670
            :.:::...:.|.  :|.:.......||.:|.:.:|..:|.|.|.|...|   .|:.:....||..
  Rat   217 SSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQT 281

  Fly   671 KMST----------------QMFLQMESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSC 719
            :::|                :..|.|.....|.|.          .|||..||..:.....||.
  Rat   282 QLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAG----------QPLPLTLPPELVPPSFRGG- 335

  Fly   720 QNLAQPNSV-GTAFNYPLVSTTTAVQNDNLNIAF-------------PLSQRPNIVQQLQQYQ-- 768
               .|.:|: ||..:|.  .|........|.:.|             .|.:|..::.:.||.:
  Rat   336 ---KQIDSINGTLPSYQ--KTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAE 395

  Fly   769 ----------QQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTS----- 818
                      :::|.::...:....|.....|  .|.|.:....:....:..:|.....|
  Rat   396 RKAQKEKEEWERKQRELQEQEWKKQLELEKRL--EKQRELERQREEERRKEIERRESCISFPLMR 458

  Fly   819 -GSWEIVEKD-----GESSPPGTPPPPY-------LSSSHMTVLEDPNENNRGAAAAGPGVFIES 870
             ..|..|.||     .|...||:.....       |....:...|..|:.||           |.
  Rat   459 EREWISVYKDRKGFMAELWKPGSCAAKQELERQRRLEWERIRRQELLNQKNR-----------EQ 512

  Fly   871 HQFTPMAGASSPIPISLHSSHMHAAQ----------SNDTQKEIISMEDENSDLD----EPFIDE 921
            .:...:......:.:.|.:.:....|          ...|||..:.:.|:..||:    :....|
  Rat   513 EEIVRLNSKKKSLHLELEAVNGKHQQISGRLQDVRIRKQTQKTELEVLDKQCDLEIMEIKQLQQE 577

  Fly   922 NGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKD-----MRKWAYEIH 981
            ...:.|....|..|.........|..:||:..:.     |:.|:|:.:.|:     :::....:.
  Rat   578 LQEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSG-----ISLLHKKSSEKEELCQRLKEQLDALE 637

  Fly   982 STFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTA 1046
            .......:.:..:..........|:|||.....:..|..:|         |:.::|||....:..
  Rat   638 KETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLF---------LLLKELRESYNTQQL 693

  Fly  1047 GLGTIYGPTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSP---------PEDVRKVALCS 1102
            .|..::....|||.|.:..:| |||..|.|        |||....         .|.:||..
  Rat   694 ALEQLHKIKRDKLKELERKRL-EQIQKKKL--------EDEAARKAKQGKENLWKESIRKEE--- 746

  Fly  1103 ALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVR----GHPLV-LRQYYEVT 1162
                                |.....:..:|| :.|...:.||...:    ...|| .|..|...
  Rat   747 --------------------EEKQKRLQEEKS-QDRTQEEERKTEAKQSETARALVNYRALYPFE 790

  Fly  1163 HCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKI---- 1223
            ..||        ....::..|    |.:...|.|.|  ||.|           ...|.||.
  Rat   791 ARNH--------DEMSFNSGD----IIQVDEKTVGE--PGWL-----------YGSFQGKFGWFP 830

  Fly  1224 -----RPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSG 1283
                 :..:||...:.|::.....:.:..|....|.....||:......|:|.   .|.:.||
  Rat   831 CNYVEKMLSSDKTPSPKKALLPPAVSLSATSAAPQPLCSNQPAPVTDYQNVSF---SNLNVNT-- 890

  Fly  1284 LNTTDLQSSFHGSCANDSINPGGGAG-----------CNM-------------DLSTSVASTTPS 1324
              |...:|:|..:.:..|::|..|.|           |:.             |:.|.:......
  Rat   891 --TWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENW 953

  Fly  1325 TSGSVAAGLSAFAEL-------NALDTVDKEARRERYSQHPKHKSAPVSVNRSESY--------- 1373
            ..|.|..|...|.:.       :.:...:.||.....::.|...:.||    .|.|
  Rat   954 WFGEVHGGRGWFPKSYVKIIPGSEVKRGEPEALYAAVNKKPTSTAYPV----GEEYIALYSYSSV 1014

  Fly  1374 ----------KERLSNKRNRN------SRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSN----- 1417
                      :|.|..:::..      ..|....||...||.|::   .:.||:..|:||
  Rat  1015 EPGDLTFTEGEELLVTQKDGEWWTGSIGERTGIFPSNYVRPKDQE---NVGNASKSGASNKKPEI 1076

  Fly  1418 ---SSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDS----- 1474
               :|..:|.|:|..|      .|||..                .|::::::..:.|.:.
  Rat  1077 AQVTSAYAASGAEQLS------LAPGQL----------------ILILKKNSSGWWQGELQARGK 1119

  Fly  1475 ------FQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVN-------------EADW 1520
                  |.|......|.||...:..::|...: :|.:...:.:||::|             :.||
  Rat  1120 KRQKGWFPASHVKLLGPSAERTTPAFHAVCQV-IAMYDYIANNEDELNFSKGQLINVMNKDDPDW 1183

  Fly  1521 --------SSMVAAEVLAALTDA-------------------EKKRQEIINEIYQTERNHVRTLK 1558
                    :.:..:..:...||:                   |:|||..|:|:.:||..::..|:
  Rat  1184 WQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPMERKRQGYIHELIETEERYMDDLQ 1248

  Fly  1559 LLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIE-----HNHVVNTIGDL 1618
            |:..:|...:.|||.|::..:.|:|    ::.:|:..:..:.||..|:.     ....|..:||:
  Rat  1249 LVIEVFQKRMAESGFLTEAEMALIF----VNWKELIMSNTKLLKALRVRKKTGGEKMPVEMMGDI 1309

  Fly  1619 LADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPT 1683
            ||.........:      :||:.|......|::|.::|...::.|||..|...|:.:.|...|..
  Rat  1310 LAAELSHMQAYI------RFCSCQLNGAALLQQKTDEDADFKEFLKKLASDPRCKGMPLSSFLLK 1368

  Fly  1684 VLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQR 1748
            .:||:|:||||..::.:.|    |:|..:..:::.|:|.::.:..:||:.|:..|::.:|:.||.
  Rat  1369 PMQRITRYPLLIRSILENT----PQNHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQA 1429

  Fly  1749 KLDRSSYDKE-EFKKLD--LTQHHLIHDGNLTIKKNPSVQLHGLLFENMIVL------------- 1797
            .:......:: .|..|.  |....|::.|.| .|...:.:|||.||.:.::|
  Rat  1430 HVQCEGLAEQLIFNSLTNCLGPRKLLYSGKL-YKTKSNKELHGFLFNDFLLLTYLVRQFAASSGF 1493

  Fly  1798 --LTKQDDKYYLKNLHTPLSI------------TNKPVSPIMSIDADTLIRQEAADKNSFFLIKM 1848
              |.........|...||:.:            :::||..|..||....:|.:..::.:.::.|:
  Rat  1494 EKLFSSKSSAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKI 1558

  Fly  1849 KTS-------------QMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALA 1900
            |.:             :..:.|:..:|.......|.  :.|.:.|....|..||....||..:
  Rat  1559 KAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHV--IEATELKACKPNGKSNPYCEISMGS-- 1619

  Fly  1901 AIPHSNTKESLELSTDTVQP 1920
               .|.|..:|:   ||:.|
  Rat  1620 ---QSYTTRTLQ---DTLNP 1633

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	19/125 (15%)
		C1_1	1151..1202	CDD:278556	11/51 (22%)
		RhoGEF	1539..1731	CDD:238091	49/196 (25%)
		PH_RhoGEF	1770..1875	CDD:275411	25/144 (17%)
		DUF2413	2242..>2378	CDD:287302
Itsn2	XP_038969030.1	EH	15..107	CDD:197477
		KLF3_N	106..>185	CDD:410554	15/74 (20%)
		EH	238..317	CDD:197477	17/88 (19%)
		PTZ00121	<347..797	CDD:173412	86/519 (17%)
		SH3_Intersectin2_1	780..836	CDD:212921	17/80 (21%)
		SH3_Intersectin2_2	921..972	CDD:212923	7/50 (14%)
		SH3_Intersectin2_3	1004..1055	CDD:212925	6/50 (12%)
		SH3_Intersectin2_4	1076..1134	CDD:212927	12/79 (15%)
		SH3_Intersectin2_5	1149..1202	CDD:212929	6/53 (11%)
		RhoGEF	1233..1413	CDD:214619	48/193 (25%)
		PH_13	1431..1574	CDD:406942	25/143 (17%)
		C2_Intersectin	1574..1708	CDD:176021	16/70 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910