DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and ARHGEF12

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_056128.1	Gene:	ARHGEF12 / 23365	HGNCID:	14193	Length:	1544	Species:	Homo sapiens

Alignment Length:	2246	Identity:	473/2246 - (21%)
Similarity:	742/2246 - (33%)	Gaps:	900/2246 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly   263 VRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTT 327
            ::||.||:|:.|||||||||:|||..|||..||:..||.|::|||..|....|..||.|||:.:.
Human    75 IQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSY 139

  Fly   328 VELAVK----------RSQKLTRPSSVS-----VVTPSTPILSGRDRTASITGPQPVDSIKRREM 377
            |.|.|:          .:.....||.:.     :.:|.:|..||  ....||.|..:.. :...:
Human   140 VALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASG--NMERITSPVLMGE-ENNVV 201

  Fly   378 ETYKIQTLQKMLEQEKLNLERLKSDQN-NPSYKL------SEANIRKLREQLHQVGAEDAPTVKL 435
            ...|::.|:|||::|:..|:.|:.|.| .|:.:|      ::.:|.:|:|||.
Human   202 HNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLS------------ 254

  Fly   436 QAAAGNKNTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRS 500
            :|....::.|::||
Human   255 KATGSAQDGAVVTP--------------------------------------------------- 268

  Fly   501 LSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQ 565
                               |.|.|.|.             ::|::
Human   269 -------------------SRPLGDTL-------------TVSEA-------------------- 281

  Fly   566 HRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDR 630
                ||.|..      ::.|:                     |..:|:||...|         |.
Human   282 ----ETDPGD------VLGRT---------------------DCSSGDASRPSS---------DN 306

  Fly   631 ATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEV 695
            |.||:....::..|..:.:             |::.:.|  ..||..:...|...|
Human   307 ADSPKSGPKERIYLEENPE-------------KSETIQD--TDTQSLVGSPSTRIA--------- 347

  Fly   696 DGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNI 760
                    |.:.|              |:.:..||
Human   348 --------PHIIG--------------AEDDDFGT------------------------------ 360

  Fly   761 VQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVE 825
                       :|:...||.  :..|:..|.|::            |.|
Human   361 -----------EHEQINGQC--SCFQSIELLKSR------------PAH---------------- 384

  Fly   826 KDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSS 890

Human   385 ----------------------------------------------------------------- 384

  Fly   891 HMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAP 955
                                                             ||:||::|:|..|||.
Human   385 -------------------------------------------------LAVFLHHVVSQFDPAT 400

  Fly   956 LLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEIL-- 1018
            ||.||.::|||...||:.|:...|.|..||...|.|.....||..|       .||..:.|::
Human   401 LLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSA-------DLEKRRPELIPE 458

  Fly  1019 ---RTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKL-REQIIDKYLMPN 1079
               |......::|....:...|.:|:|||:.||         .|||::..|| .|:..|:
Human   459 DLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGL---------TLAESELTKLDAERDKDR----- 509

  Fly  1080 LHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNR 1144
              ..:|.|.....:.|.|:.  ..|.|.                   ..|..|||
Human   510 --LTLEKERTCAEQIVAKIE--EVLMTA-------------------QAVEEDKS---------- 541

  Fly  1145 KMNVRGHPLVLRQYYEVTHCNHCQTIIWGV---SPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQ 1206
                     ...||..:.:..|.     ||   .|:         |:..:..::      |.||:
Human   542 ---------STMQYVILMYMKHL-----GVKVKEPR---------NLEHKRGRI------GFLPK 577

  Fly  1207 AKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISI 1271
            .|:....||               .|.||..|            ||..||:..|  |||
Human   578 IKQSMKKDK---------------EGEEKGKR------------RGFPSILGPP--RRP------ 607

  Fly  1272 RSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAF 1336
                                |.|.:.|         .|..|:|
Human   608 --------------------SRHDNSA---------IGRAMEL---------------------- 621

  Fly  1337 AELNALDTVDKEARRERYSQHPKHKSAPVSVN--RSESYKERLSNKRNRNSRRKTSDPSLSSRPN 1399
                      ::||      ||||.|.|.||:  ..:|.|.|.|...|
Human   622 ----------QKAR------HPKHLSTPSSVSPEPQDSAKLRQSGLAN----------------- 653

  Fly  1400 DEQLDLGLSNATYVGS--SNSSLSSAGGSESPSTSME--HFAAPG-AAGGV--------QVPPMG 1451
             |..|.|...|..:.|  |.:|.|..||.|:.:.|.:  ..:||| ...|.        ..||..
Human   654 -EGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPP 717

  Fly  1452 LNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLP------VARWTLESE 1510
            |:|.|.....:::..:.                            |.|.|      ||....:||
Human   718 LDQVQEEECEVERVTEH----------------------------GTPKPFRKFDSVAFGESQSE 754

  Fly  1511 DEDDVNEAD-----WSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYE 1570
            ||...|:.:     |..:|:.|||..|...|.||||:|||::.|||.||||||:||::|:..:..
Human   755 DEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSR 819

  Fly  1571 SGLLSQDHLLLLFPPALLSLREIH-GAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEF 1634
            .|:||...|..:| ..|..:.::| |..||....|:.....|::.||:.|...|.|.....|...
Human   820 EGILSPSELRKIF-SNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHA 883

  Fly  1635 AAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLY 1699
            ||.||:.|..|||.:|.::.||...|..::.:||:..||||:|||::||.:|||||||||.:|:.
Human   884 AATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIA 948

  Fly  1700 KVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSY------DKE 1758
            |.|     |..||.|.:::|.:..::||..||||||.||:..:|::.||:||.||.      :.|
Human   949 KYT-----EWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVE 1008

  Fly  1759 EFKKLDLTQHHLIHDGNLT--IKKNPSVQLHGLLFENMIVLLTKQDDKYYLKNLHTPL----SIT 1817
            |.:.||||:..:||:|.|.  :.::.::.|:.||.|:::|||.||||:..|: .|:.:    :.:
Human  1009 ELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLR-CHSKILASTADS 1072

  Fly  1818 NKPVSPIMSIDADTLIRQEAADKNSFFLIKMKT--SQMLELRAPSSSECKTWFKHFSDVAARQSK 1880
            ....||::.: :..|:||.|.|..:.|:|.|..  :|:.||.|.:.||...|    .|:..|.:
Human  1073 KHTFSPVIKL-STVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVW----QDLICRMA- 1131

  Fly  1881 NRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAP 1945
                               |::...:||.                          :|:...||..
Human  1132 -------------------ASVKEQSTKP--------------------------IPLPQSTPGE 1151

  Fly  1946 ATNN----------SNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGE 2000
            ..|:          .:..|:||:|  :|.||.....:    :.:.||:|.        |:.::|:
Human  1152 GDNDEEDPSKLKEEQHGISVTGLQ--SPDRDLGLEST----LISSKPQSH--------SLSTSGK 1202

  Fly  2001 PIQKYSANGTEANDVTLRHSQSTRESVRPGSTGEERNSTYGMVGGNSKRDSASIVCSNNSNNTRT 2065
                     :|..|:.:...|..:|....|:..|        ||.:
Human  1203 ---------SEVRDLFVAERQFAKEQHTDGTLKE--------VGED------------------- 1231

  Fly  2066 LLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQKIICDI------FRLPV 2124
                        .|::|..:|     |...|:...||| :||..|.||.::..:      .:...
Human  1232 ------------YQIAIPDSH-----LPVSEERWALDA-LRNLGLLKQLLVQQLGLTEKSVQEDW 1278

  Fly  2125 EHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEK 2189
            :|:.:....:..|:.            |.:...::.:..| |...........|.....|:....
Human  1279 QHFPRYRTASQGPQT------------DSVIQNSENIKAY-HSGEGHMPFRTGTGDIATCYSPRT 1330

  Fly  2190 LRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLM--PKLQTLDLDEVAIHEDDDGYCEIDE 2252
            ..:..||..|...:|        |.:||...:....::  |::.|:        |.:.|..:..|
Human  1331 STESFAPRDSVGLAP--------QDSQASNILVMDHMIMTPEMPTM--------EPEGGLDDSGE 1379

  Fly  2253 LRLPAIPSKPHERPTTPLAPFNTEPKTSQSVI----------DASKRQSTDAVPEGLLEQEPLEG 2307
            ....|..:...|.|:......|.|.|.....|          |..:..|||......|..:|:.|
Human  1380 HFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTG 1444

  Fly  2308 DKT--ETKGEDNEVKTVPSD-KLSESCNE----------ERQCVEADITKEVADPTTSKNEAAAS 2359
            ...  .|..:.:..:...|| .:|....|          |..|.|.......||   |:::....
Human  1445 IPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCAD---SQSQIMEY 1506

  Fly  2360 VDELPSQSREIKTAEN---------ASKSVADKKED 2386
            :.::.:....:|..|.         |..::.||..|
Human  1507 IHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSD 1542

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	38/80 (48%)
		RGS_GEF_like	934..1055	CDD:188710	40/125 (32%)
		C1_1	1151..1202	CDD:278556	7/53 (13%)
		RhoGEF	1539..1731	CDD:238091	78/192 (41%)
		PH_RhoGEF	1770..1875	CDD:275411	35/112 (31%)
		DUF2413	2242..>2378	CDD:287302	31/167 (19%)
ARHGEF12	NP_056128.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..62
		PDZ_signaling	71..145	CDD:238492	37/69 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	247..346		32/268 (12%)
		RGS_LARG	349..570	CDD:188708	76/496 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	570..706		55/261 (21%)
		RhoGEF	791..976	CDD:279015	76/190 (40%)
		PH_LARG	995..1132	CDD:275425	45/162 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1138..1179		11/68 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	138	1.000	Domainoid score	I4844
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	332	1.000	Inparanoid score	I2429
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D163264at33208
OrthoFinder	1	1.000	-	-		FOG0001574
OrthoInspector	1	1.000	-	-		otm41901
orthoMCL	1	0.900	-	-		OOG6_106011
Panther	1	1.100	-	-	O	PTHR45872
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3275
SonicParanoid	1	1.000	-	-		X2399
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.860