DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and AKAP13

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	NP_006729.4	Gene:	AKAP13 / 11214	HGNCID:	371	Length:	2817	Species:	Homo sapiens

Alignment Length:	2819	Identity:	536/2819 - (19%)
Similarity:	920/2819 - (32%)	Gaps:	903/2819 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 EHEYDEVQEIP---------EESSIQP-PETSTSHT----------STNGSSHSG---------- 51
            |.:.:||:..|         ::|.:|. |:.....|          ...|:..||
Human   377 ERKGEEVEPAPIVDSGTVSDQDSCLQSLPDCGVKGTEGLSSCGNRNEETGTKSSGMPTDQESLSS 441

  Fly    52 ------------PGTATGPGATSAGPSAGAPQSPVIVVDSVPEL-------PAPKQKSVKNSKSK 97
                        ||||.   .:|.|...|...:.|...|:..|:       .|...|:|......
Human   442 GDAVLQRDLVMEPGTAQ---YSSGGELGGISTTNVSTPDTAGEMEHGLMNPDATVWKNVLQGGES 503

  Fly    98 QKQK------------------QLANKSKIPRSPSLASSL-------SSLASS---LSGHRDRDK 134
            .|::                  :..:|..:|.....||||       ||||.|   .|..:..:.
Human   504 TKERFENSNIGTAGASDVHVTSKPVDKISVPNCAPAASSLDGNKPAESSLAFSNEETSTEKTAET 568

  Fly   135 DRDKDRE-------NQNAVPPQTPPLPPSYKQNQMNG--DSTAAAGG-------------GVSAP 177
            :..:.||       :||:|     .:|.:.|....:|  ..|..|.|             |:...
Human   569 ETSRSREESADAPVDQNSV-----VIPAAAKDKISDGLEPYTLLAAGIGEAMSPSDLALLGLEED 628

  Fly   178 ATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGE-------RSSLNLTPLSRDLSGG-- 233
            ..|...:..|:||..          :|...|:|:....|:       ....|....|.||...
Human   629 VMPHQNSETNSSHAQ----------SQKGKSSPICSTTGDDKLCADSACQQNTVTSSGDLVAKLC 683

  Fly   234 ----HTQESTTPATTPST---PSLALPKNFQYLTLT---VRK-------------DSNGYGMKVS 275
                ...|||| |..||:   |..:..::.|..|:|   ||.             |:.|....|.
Human   684 DNIVSESESTT-ARQPSSQDPPDASHCEDPQAHTVTSDPVRDTQERADFCPFKVVDNKGQRKDVK 747

  Fly   276 GDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTTVELAVKRSQKLTR 340
            .|.|:                        .|..||....||.|
Human   748 LDKPL------------------------TNMLEVVSHPHPVV---------------------- 766

  Fly   341 PSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERLKSD-QN 404
            |.....:.|...::|  |.|.|:......:|:.:.:..::.....:|.....:|:   ..:| ::
Human   767 PKMEKELVPDQAVIS--DSTFSLANSPGSESVTKDDALSFVPSQKEKGTATPELH---TATDYRD 826

  Fly   405 NPSYKLSEANIRKLREQLHQVGAEDAPT-VKLQAAAGNKNTALLTPNQIQHLSASATHSNQQFHH 468
            .|....:|.:.|.|.::  .||...:.| .:||...||.:...|          ...|.......
Human   827 GPDGNSNEPDTRPLEDR--AVGLSTSSTAAELQHGMGNTSLTGL----------GGEHEGPAPPA 879

  Fly   469 LHHHHNLHNNNYPPQQQPASTSPAFLSLLPRS----LSSLSLGTRKNKTEKDLTTSS--PFGLTT 527
            :....|:..|.....|.....:.|..|:|...    :.|.|...::...:|.:|.||  ...|::
Human   880 IPEALNIKGNTDSSLQSVGKATLALDSVLTEEGKLLVVSESSAAQEQDKDKAVTCSSIKENALSS 944

  Fly   528 DFLQQQRMSHQAESMSQSMH--------------QHTSTPTSQQFFHPHQQQHRFKETGPTSKGK 578
            ..||:::.:......:|..|              |.:::|.:...|...:.:|. ||..|    :
Human   945 GTLQEEQRTPPPGQDTQQFHEKSISADCAKDKALQLSNSPGASSAFLKAETEHN-KEVAP----Q 1004

  Fly   579 NKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNAS------PGGSHLVAPVSDLDRATSPQLN 637
            ...|......:..|||........||..|..::.:::      |.||.           .|..||
Human  1005 VSLLTQGGAAQSLVPPGASLATESRQEALGAEHNSSALLPCLLPDGSD-----------GSDALN 1058

  Fly   638 RSQQQQLPSSTDNSPSNAKSKRSKIK------------TKALSDPKM-----STQ------MFLQ 679
            .||...|.....|:.|..|:...::.            .:.:::|:.     :||      :.|.
Human  1059 CSQPSPLDVGVKNTQSQGKTSACEVSGDVTVDVTGVNALQGMAEPRRENISHNTQDILIPNVLLS 1123

  Fly   680 MESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPL-------- 736
            .|..:..|              ||..|......|           |..|||....||
Human  1124 QEKNAVLG--------------LPVALQDKAVTD-----------PQGVGTPEMIPLDWEKGKLE 1163

  Fly   737 -------VSTTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNK 794
                   :......|.|  :.|.|:..:| :.::|....:...|..........:.:.|..|:.:
Human  1164 GADHSCTMGDAEEAQID--DEAHPVLLQP-VAKELPTDMELSAHDDGAPAGVREVMRAPPSGRER 1225

  Fly   795 HRR----VGSSPDNMHPRHPDRITKTTSGSWEIV------------------------------- 824
            ...    :.|:.|...|:..|.|.:..|...:.|
Human  1226 STPSLPCMVSAQDAPLPKGADLIEEAASRIVDAVIEQVKAAGALLTEGEACHMSLSSPELGPLTK 1290

  Fly   825 -------EK-----DGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMA 877
                   ||     .|||.|.|:.|                |...|:.|   |.|         |
Human  1291 GLESAFTEKVSTFPPGESLPMGSTP----------------EEATGSLA---GCF---------A 1327

  Fly   878 GASSP----IPI-----SLHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTRLLE 933
            |...|    :|:     :.....:.|....|.:...||.|.....:......:.||   :|:.:.
Human  1328 GREEPEKIILPVQGPEPAAEMPDVKAEDEVDFRASSISEEVAVGSIAATLKMKQGP---MTQAIN 1389

  Fly   934 AENVTFLAIFLNYVISNSDPAP----LLF---------YLITELYKEGTSK------------DM 973
            .||...:           :|.|    ||.         :|...|.:|.|||            |:
Human  1390 RENWCTI-----------EPCPDAASLLASKQSPECENFLDVGLGRECTSKQGVLKRESGSDSDL 1443

  Fly   974 RKWAYEIHSTFLVPRAPLSWYRQDESLAREV-------DNVLQL--------EYDKVEILRTVFL 1023
            .....:...:.:.|:.      ::|.||.::       |:...|        :....:||:....
Human  1444 FHSPSDDMDSIIFPKP------EEEHLACDITGSSSSTDDTASLDRHSSHGSDVSLSQILKPNRS 1502

  Fly  1024 RSRKRAKDLISEQL----REFQQKRTAGLGTIYGPTDDKLAE--AKTDKLREQIIDKYLMP-NLH 1081
            |.|:......|..:    || .:...|..|.:.....|.:.|  |....||..:  :.|.| ..|
Human  1503 RDRQSLDGFYSHGMGAEGRE-SESEPADPGDVEEEEMDSITEVPANCSVLRSSM--RSLSPFRRH 1564

  Fly  1082 ALIEDENGSPPEDV--RKVALC-------SALSTVI-YRIFNT----------RPPPSSI----- 1121
            :....:|.:...::  |.::.|       :.||... ||.|:.          ..|.||:
Human  1565 SWGPGKNAASDAEMNHRSMSWCPSGVQYSAGLSADFNYRSFSLEGLTGGAGVGNKPSSSLEVSSA 1629

  Fly  1122 -VERVHHFVSRDKSFKS--RIMGKNRKMNVRGHPLVLRQYYEVTH------CNHCQTII------ 1171
             .|.:.|..|.::...|  .:..::.:.:.|.|.:..:|   :.|      .|:|.:.|
Human  1630 NAEELRHPFSGEERVDSLVSLSEEDLESDQREHRMFDQQ---ICHRSKQQGFNYCTSAISSPLTK 1691

  Fly  1172 ----WGVSPQG----------YHCTDCKLNIHRQCSKVVDES---CP-GPLPQAKRLAHNDKISK 1218
                ..:|..|          :|.|...|.     ..:.:|:   .| .|..:........|:|:
Human  1692 SISLMTISHPGLDNSRPFHSTFHNTSANLT-----ESITEENYNFLPHSPSKKDSEWKSGTKVSR 1751

  Fly  1219 FMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNT------ 1277
            ....|:.:.|    :.|:|::.|         :.:..|..:..|.:.........||:|
Human  1752 TFSYIKNKMS----SSKKSKEKE---------KEKDKIKEKEKDSKDKEKDKKTVNGHTFSSIPV 1803

  Fly  1278 ----SCNTSGLNTTDLQSSFHGSCANDS--INPGGGAGCNMDLSTSVASTTPSTSGSVAAGLSAF 1336
                ||:......|:..:.   :|||.|  ::    .||...|::..........||:.|
Human  1804 VGPISCSQCMKPFTNKDAY---TCANCSAFVH----KGCRESLASCAKVKMKQPKGSLQA----- 1856

  Fly  1337 AELNALDTVDKEARRERYSQHPKHK------------SAPVSVNRSESYKERLSNKRNRNSRRKT 1389
            .:.::|.||   ..|.:.|| ||.:            :.|:..||.......||        :..
Human  1857 HDTSSLPTV---IMRNKPSQ-PKERPRSAVLLVDETATTPIFANRRSQQSVSLS--------KSV 1909

  Fly  1390 SDPSLSSRPNDEQLDL----------GLSNATYVGSSNSSLSSAG-GSESPSTSMEHFAAPGAAG 1443
            |..:::...|||.:..          .|:..:.|..|..||:..| |::
Human  1910 SIQNITGVGNDENMSNTWKFLSHSTDSLNKISKVNESTESLTDEGVGTD---------------- 1958

  Fly  1444 GVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLE 1508
                    :|:.|                      |.|                          :
Human  1959 --------MNEGQ----------------------LLG--------------------------D 1967

  Fly  1509 SEDEDDVNEAD-WSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESG 1572
            .|.|....||: ||.::.::.|........||||:|.|:.|||.:||||||::..::...:....
Human  1968 FEIESKQLEAESWSRIIDSKFLKQQKKDVVKRQEVIYELMQTEFHHVRTLKIMSGVYSQGMMADL 2032

  Fly  1573 LLSQDHLLLLFPPALLSLREIHGAFEQSLKQR-------RIEHNHVVNTIGDLLADMFDGQSGVV 1630
            |..|..:..|| |.|..|..||..|.|.:.:|       :.|.|.::..|||:|.:.|.|::...
Human  2033 LFEQQMVEKLF-PCLDELISIHSQFFQRILERKKESLVDKSEKNFLIKRIGDVLVNQFSGENAER 2096

  Fly  1631 LCEFAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLF 1695
            |.:...:||.:...::...|:...||:..|..:||..|....|||.:.:.:..|.||:||||:||
Human  2097 LKKTYGKFCGQHNQSVNYFKDLYAKDKRFQAFVKKKMSSSVVRRLGIPECILLVTQRITKYPVLF 2161

  Fly  1696 ENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK--- 1757
            :.:.:.|    .:|..|.|.:.:::...|.::..|:..|.:.|...:|..|..|.|..|..:
Human  2162 QRILQCT----KDNEVEQEDLAQSLSLVKDVIGAVDSKVASYEKKVRLNEIYTKTDSKSIMRMKS 2222

  Fly  1758 -EEFKKLDLTQHHLIHDGNLTIKKNPS--VQLHGLLFENMIVLLTKQDDKYYLKNLHTPLSITNK 1819
             :.|.|.||.:..|:.||::.:|....  .::..:|..:::|.|.::|.||...:|...
Human  2223 GQMFAKEDLKRKKLVRDGSVFLKNAAGRLKEVQAVLLTDILVFLQEKDQKYIFASLDQK------ 2281

  Fly  1820 PVSPIMSIDADTLIRQEAADKNSFFLIKMKTS--QMLELRAPSSSECKTWFKHFSDVAARQSKNR 1882
              |.::|: ...::|:.|.::...|||.|..:  :|:|:.|.|..|..:|.:...|.....:::.
Human  2282 --STVISL-KKLIVREVAHEEKGLFLISMGMTDPEMVEVHASSKEERNSWIQIIQDTINTLNRDE 2343

  Fly  1883 SKNASSNHDTS--------------------------------ISDPALAAIP------------ 1903
            .:...|.::..                                ..|.|..:.|
Human  2344 DEGIPSENEEEKKMLDTRARELKEQLHQKDQKILLLLEEKEMIFRDMAECSTPLPEDCSPTHSPR 2408

  Fly  1904 ---HSNTKESLE---LSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVSSLTG-VQLR 1961
               .|||:|:|:   |....:..:.....|.:..|...|       .|..::.....:.| |.|
Human  2409 VLFRSNTEEALKGGPLMKSAINEVEILQGLVSGNLGGTL-------GPTVSSPIEQDVVGPVSL- 2465

  Fly  1962 NPQRDATASESDADYVNTPKPRSSQ-----NEVNRTMSIRSTGEPIQKYSANGTEANDVTL--RH 2019
             |:|..|....|:..:|..|....:     .::.||.|  .:|  ::|    |..||.|.:  |:
Human  2466 -PRRAETFGGFDSHQMNASKGGEKEEGDDGQDLRRTES--DSG--LKK----GGNANLVFMLKRN 2521

  Fly  2020 SQSTRESVRPGSTGEERNSTYGMV-GGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSIS 2083
            |:...:||  ....|..::..|:| ..:|..:...:|.|..: .||:|...|.|::.
Human  2522 SEQVVQSV--VHLYELLSALQGVVLQQDSYIEDQKLVLSERA-LTRSLSRPSSLIEQ-------- 2575

  Fly  2084 PAHTAEPVLTPGEKLRRLDASIRNDL--LEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADI 2146
                        ||.|.|:.. |.||  |:||:.             |.::.....|...::.:.
Human  2576 ------------EKQRSLEKQ-RQDLANLQKQQA-------------QYLEEKRRREREWEARER 2614

  Fly  2147 ALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPN 2211
            .|...:.:  |.:...|......:.|            .|:|:|::|   ..::......|....
Human  2615 ELREREAL--LAQREEEVQQGQQDLE------------KEREELQQK---KGTYQYDLERLRAAQ 2662

  Fly  2212 RQHAQAQAQI--PPSRLMPKLQTLDLDEVAIHEDDDGYCEIDE-----LRLPAIPSKPHERPTTP 2269
            :|..:.|.|:  ...||..:....||            |::..     :|:|:....| |.|.:|
Human  2663 KQLEREQEQLRREAERLSQRQTERDL------------CQVSHPHTKLMRIPSFFPSP-EEPPSP 2714

  Fly  2270 LAPFNTEPKTSQSVIDAS-KRQS-------------------TDAVPEGLLEQEP--------LE 2306
            .||...:..:..|.:..| ||.|                   |:..||| ..|.|        |.
Human  2715 SAPSIAKSGSLDSELSVSPKRNSISRTHKDKGPFHILSSTSQTNKGPEG-QSQAPASTSASTRLF 2778

  Fly  2307 G-----DKTETKGEDNEVKTVPSD 2325
            |     :|.|.|.::...::.|.|
Human  2779 GLTKPKEKKEKKKKNKTSRSQPGD 2802

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	15/89 (17%)
		RGS_GEF_like	934..1055	CDD:188710	28/164 (17%)
		C1_1	1151..1202	CDD:278556	13/80 (16%)
		RhoGEF	1539..1731	CDD:238091	61/198 (31%)
		PH_RhoGEF	1770..1875	CDD:275411	26/108 (24%)
		DUF2413	2242..>2378	CDD:287302	27/122 (22%)
AKAP13	NP_006729.4	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	304..400		4/22 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	415..439		3/23 (13%)
		Important for interaction with PRKAR2A	494..516		3/21 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	539..585		12/45 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..653		6/30 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	690..726		13/36 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	805..856		10/55 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..965		4/25 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1431..1455		1/23 (4%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1467..1542		12/75 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1605..1642		7/36 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1759..1797		8/50 (16%)
		C1	1796..1842	CDD:294036	11/52 (21%)
		Interaction with ESR1. /evidence=ECO:0000269\|PubMed:9627117	1923..2817		220/1051 (21%)
		RhoGEF	1999..2193	CDD:238091	61/198 (31%)
		PH_AKAP13	2236..2338	CDD:275427	26/110 (24%)
		PH	2236..2336	CDD:278594	26/108 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2470..2506		8/39 (21%)
		RILP-like	<2630..>2700	CDD:304877	14/96 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2669..2817		33/148 (22%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810