DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and Arhgef28

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_006517581.1	Gene:	Arhgef28 / 110596	MGIID:	1346016	Length:	1714	Species:	Mus musculus

Alignment Length:	2030	Identity:	392/2030 - (19%)
Similarity:	652/2030 - (32%)	Gaps:	644/2030 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   493 FLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQ 557
            ||..||..:.:|.|...:..|..||..........:.:...:.||........:::..:.    |
Mouse   195 FLLCLPGGVKALKLPNEEATTPLDLALQGGHSTLVEDITNFQGSHSPGFSRLRLNEEATL----Q 255

  Fly   558 FFHP---------HQQQHRFKETGPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGN 613
            |.|.         |..:|..:   ...|...|:...|:.:             .:.|..:.|...
Mouse   256 FVHSSETLTLTVNHTAEHLLE---ADIKLFRKYFWDRAFL-------------VKALEQEAKTEK 304

  Fly   614 AS--PGGSHLVAPVSDLDRATSPQLNRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQM 676
            |:  .|.:.....|.:|:...||.......:.:.|..| .||..:.:......::....|.|..:
Mouse   305 ATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVD-GPSEHEDQDRLPLDRSFDGLKKSKHV 368

  Fly   677 FLQMESASAAGAAGGSIE--VDGGPPPLPPRLPGMMTEDMSRGSCQNLAQPNSVGTAF-NYPLVS 738
                    .|..|.|.:.  ::||              |....:|..:.|...:...: |...:|
Mouse   369 --------PASLAAGQLSDVLNGG--------------DEVYANCMVIDQVGDLDINYINLEGLS 411

  Fly   739 TTTAVQNDNLNIAFPLSQRPNIVQQLQQYQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPD 803
            |.|:          |.|.|                .|.|.||.
Mouse   412 THTS----------PESGR----------------SMLGPQAC---------------------- 428

  Fly   804 NMHPRHPDRITKTTSGSWEIVEKDGESSPPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFI 868
             ||...||                  :||.|.|            |.:.:|....|||
Mouse   429 -MHTLPPD------------------TSPCGRP------------LIENSEGTLDAAA------- 455

  Fly   869 ESHQF-TPMAGASSPIPIS--LHSSHMHAAQSNDTQKEIISMEDENSDLDEPFIDENGPFNNLTR 930
             |..| ||.:..:|.:.:|  ||.          .:||...::..:|.||....|..|...:...
Mouse   456 -SQSFVTPSSSRTSNLNLSFGLHG----------FEKEQSHLKKRSSSLDALVADSEGEGGSEPP 509

  Fly   931 LLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYR 995
            :..|            |.|.|.|...|        ..|...|    ::|.::.   |...:|
Mouse   510 ICYA------------VGSQSSPRTGL--------PSGDELD----SFETNTE---PDCNIS--- 544

  Fly   996 QDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLI----SEQLREFQQKRTAGLGTIYG--- 1053
            :.|||:  :.:.|   :.|..:|..:..||...:...|    |..:|:|....|:.....|.
Mouse   545 RTESLS--LSSTL---HSKESLLSGIRSRSYSCSSPKISSGKSRLVRDFTVCSTSEEQRSYSFQE 604

  Fly  1054 PTDDKLAEAKTDKLREQIIDKYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPP 1118
            |..:|       :::|:..|:|::|   |..|.|         |..:....|.::.|:  |.|
Mouse   605 PPGEK-------RIQEEEWDEYVIP---AKSESE---------KYKVSRTFSFLMNRM--TSP-- 646

  Fly  1119 SSIVERVHHFVSRDKS-FKSRIMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQGYHCT 1182
                        |:|| .|::...:..|||  .|..|...:..|..|:.|...:.|  .:...|.
Mouse   647 ------------RNKSKMKNKDTKEKEKMN--RHQFVPGTFSGVLQCSGCDKTLLG--KESLQCA 695

  Fly  1183 DCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVEL 1247
            :||.|.|:.|...|.       |..|         ||..|
Mouse   696 NCKANTHKGCKDAVP-------PCTK---------KFQEK------------------------- 719

  Fly  1248 TPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNM 1312
                                                .|....||....|...|...||
Mouse   720 ------------------------------------YNKNKPQSILGSSSVRDVPAPG------- 741

  Fly  1313 DLSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQ-HPKHKSAPVSVNRSESYKER 1376
                  .|..||:  |:..||.|             .|:|..:| ||..:|.|.:.  .||::..
Mouse   742 ------LSLHPSS--SMPIGLPA-------------GRKEFAAQVHPLSRSVPGTT--LESFRRA 783

  Fly  1377 LSNKRNRNSRRKTSDPSLSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGA 1441
            :::..:...       |..||.:.::|                ..|.|  .||||
Mouse   784 VTSLESEGD-------SWRSRSHSDEL----------------FQSMG--SSPST---------- 813

  Fly  1442 AGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWT 1506
                                     :.:..:|...:.|                         |.
Mouse   814 -------------------------ESFMMEDVVDSSL-------------------------WI 828

  Fly  1507 LESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYES 1571
            ..|.|..:.....||.:|.....:.......|||::|.|:.|||.:|::||.::..:|...:.|.
Mouse   829 DLSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEE 893

  Fly  1572 GLLSQDHLLL--LFPPALLSLREIHGAFEQSLKQRRIE------HNHVVNTIGDLLADMFDGQSG 1628
              |..||..:  :| |.|..|.|.|..|..|:|:||.|      .|.|:|.|||:|...|..::.
Mouse   894 --LQLDHSTVDKIF-PCLDELLETHRHFFFSMKERRQESCAGSDRNFVINQIGDILVQQFSEENA 955

  Fly  1629 VVLCEFAAQFCARQQIALEALKE-KRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYP 1692
            ..:.....:||:..:.|:...|| ::||  ..|..:|...|:...||..:.:.:..|.||:||||
Mouse   956 SKMKRIYGEFCSHHKEAMSLFKELQQNK--KFQNFIKIRNSNLLARRRGIPECILLVTQRITKYP 1018

  Fly  1693 LLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDK 1757
            :|.|.:.:.|    .|.|.|...:.:|:...|.::..|:..|...|...|...|..|::..:|.|
Mouse  1019 VLVERILQYT----KERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTK 1079

  Fly  1758 ----EEFKKLDL--TQHHLIHDGNLTIKKNPS---VQLHGLLFENMIVLLTKQDDKYYLKNLHTP 1813
                ..|:|..|  .:..|:||| |...|..:   ..:..||..::::.|.::|.||..
Mouse  1080 LKNGHVFRKQALLSQERALLHDG-LVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIF------ 1137

  Fly  1814 LSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTS--QMLELRAPSSSECKTWFKHFSD-VA 1875
            .::..||  .::|:. ..:.|:.|.::...|||...::  :|.|:...|..|...|.:.... |.
Mouse  1138 AAVDQKP--SVISLQ-KLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVE 1199

  Fly  1876 ARQSKNRSKNASSNHDTSISDPALAAIPH-----SNTKES----LELSTDTVQPLAATATLTTTP 1931
            :...:...:.:.|:.:...::..:|.|..     ||..:.    ||........|...:......
Mouse  1200 SCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVH 1264

  Fly  1932 LAPMLPIATVTPAPATNNSNVSSLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRT---- 1992
            |.|.|   .:.|.|. .....:||....||           :|:.:......|...:|:::
Mouse  1265 LEPHL---LIKPDPG-EPPQAASLLAAALR-----------EAESLQVAVKASKMGDVSQSSEES 1314

  Fly  1993 ------MSIRST----GEPIQKYSANGTEAN-----DVTLRHSQSTRESVRPGSTGEERNSTYGM 2042
                  |...||    ..|.......|||..     |..|   |.....:.....||:  ..|..
Mouse  1315 PGGTVLMDTPSTQDVPASPTASLVTEGTEGRGCWDVDPGL---QGVVTDLAVSDAGEK--VEYRS 1374

  Fly  2043 VGGNSKRDSASIVCSNNSNNTRTLL-MQSPL-VDPTAIQV---------SISPAHT--AEPVLTP 2094
            ..|:|:.:....:    .|.||.|. :|:.| :..:.|::         |::|:|:  ..| |..
Mouse  1375 FSGSSQSEIIQAI----QNLTRLLYSLQAALTIQDSHIEIHKLVLQQRESLAPSHSFRGGP-LQD 1434

  Fly  2095 GEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDS---------------- 2143
            .||.|.|:.. |.:|....|:... |:.....:.:..|.....:..::|
Mouse  1435 QEKSRYLEKQ-REELANIHKLQHQ-FQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQEELL 1497

  Fly  2144 ------ADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHC-HEKEKLRKKVAPSSSFS 2201
                  .|..|..|.  |:|.::......|..|::...|...:.||| |.:::
Mouse  1498 LRHRSELDHQLQEYQ--QSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQR------------ 1548

  Fly  2202 SSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERP 2266
            |.|....|.:::                :..|:..|...||:.
Mouse  1549 SLPAVFSPGSKE----------------VTELNRAESLCHENS---------------------- 1575

  Fly  2267 TTPLAPFNTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEG---DKTETKGEDNEVK---TVPSD 2325
                  |..........::.|.:.:...||   .:..||||   |...|.....|:|   :.|.|
Mouse  1576 ------FFINEAFGHMSLNTSNKPNPSGVP---WDAHPLEGSHFDLARTSESPTELKIDISQPPD 1631

  Fly  2326 KLSE----SCNEERQCVE---ADITKEVADPTTSKNEAAASVD--ELPSQSREIKTAENASKSVA 2381
            ..||    ....:|..::   .:..|.|||..:.::|:::..|  :...|.:.:.|....|..:|
Mouse  1632 VNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLPMA 1696

  Fly  2382 -----DKKEDNEETI 2391
                 |..:..||.|
Mouse  1697 AGHGGDAGDGAEENI 1711

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570
		RGS_GEF_like	934..1055	CDD:188710	25/127 (20%)
		C1_1	1151..1202	CDD:278556	13/50 (26%)
		RhoGEF	1539..1731	CDD:238091	62/200 (31%)
		PH_RhoGEF	1770..1875	CDD:275411	25/110 (23%)
		DUF2413	2242..>2378	CDD:287302	26/150 (17%)
Arhgef28	XP_006517581.1	C1	666..711	CDD:237996	13/53 (25%)
		RhoGEF	861..1053	CDD:238091	62/200 (31%)
		PH_p190RhoGEF	1098..1198	CDD:275430	25/109 (23%)
		xseA	<1364..1520	CDD:234714	32/166 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810