DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF2 and arhgef11

DIOPT Version :9

Sequence 1:	NP_477317.1	Gene:	RhoGEF2 / 36915	FlyBaseID:	FBgn0023172	Length:	2559	Species:	Drosophila melanogaster
Sequence 2:	XP_017952119.2	Gene:	arhgef11 / 100489979	XenbaseID:	XB-GENE-992398	Length:	1607	Species:	Xenopus tropicalis

Alignment Length:	2311	Identity:	488/2311 - (21%)
Similarity:	768/2311 - (33%)	Gaps:	896/2311 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly   263 VRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVVGLIKASTT 327
            |::|.||:|..||||..|.|:||:|||||..||:..||.|::|||..|....|..||.|||:...
Frog    67 VQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAY 131

  Fly   328 VELAVKRS-----------QKLT---RPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREME 378
            |.|.:..|           |.|:   .|.::||:....|......:...||||:|   ::..:.:
Frog   132 VALTLLGSPPPSMGISGYPQDLSTSVSPRNISVLPMPPPPPPPLPQMHHITGPKP---LQDPDFQ 193

  Fly   379 TYKIQTLQKMLEQEKLNLERLKSDQN-NPSYKLSEANIRKLREQLHQVGAEDAPTVKLQAAAGNK 442
            .:..:.|..||:||:.:|:.|....| :|:..|.| .|.:.|:::||:
Frog   194 KHATEILWNMLKQEEADLQNLCDVYNKSPNSSLQE-QIERARKRVHQL----------------- 240

  Fly   443 NTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPPQQQPASTSPAFLSLLPRSLSSL-SL 506
                                         .|.:.      |:.|.:..      |.||.|.. ||
Frog   241 -----------------------------QHKIR------QEIPGTGD------LLRSYSDCSSL 264

  Fly   507 GTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMHQHTSTPTSQQFFHPHQQQHRFKET 571
            |                                                          ::.||
Frog   265 G----------------------------------------------------------YKVKE- 270

  Fly   572 GPTSKGKNKFLISRSLIEEDVPPPLPQRNPPRQLNLDLKNGNASPGGSHLVAPVSDLDRATSPQL 636
                                           .:|::|.::|:            |.||..|
Frog   271 -------------------------------GRLSVDSQDGD------------SGLDSGT---- 288

  Fly   637 NRSQQQQLPSSTDNSPSNAKSKRSKIKTKALSDPKMSTQMFLQMESASAAGAAGGSIEVDGGPPP 701
                 :::.|.::.|.:         :...|||        |.|:|:..:             |.
Frog   289 -----ERISSLSEYSMN---------RNSVLSD--------LGMDSSHTS-------------PI 318

  Fly   702 LPPRLPGMMTEDMSRGSCQNLAQPNSVGTAFNYPLVSTTTAVQNDNLNIAFPLSQRPNIVQQLQQ 766
            :|.:|
Frog   319 IPTKL------------------------------------------------------------ 323

  Fly   767 YQQQQQHQMSGGQATGALGQTPNLGKNKHRRVGSSPDNMHPRHPDRITKTTSGSWEIVEKDGESS 831
            :.....|                   :.|||.||..
Frog   324 FHHHYHH-------------------HHHRRQGSDT----------------------------- 340

  Fly   832 PPGTPPPPYLSSSHMTVLEDPNENNRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQ 896
                                              .||.:.:                       |
Frog   341 ----------------------------------AFIATSE-----------------------Q 348

  Fly   897 SNDTQKEIISMEDENSDLDEPFIDE-NGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYL 960
            .:|..|..|...:|:.|......:: :|.|.:|.||  ......:.:||.|:.|.:||.||||||
Frog   349 VSDPMKSTIICPEEDYDHGYYMTEQLDGLFQDLERL--KTRPAHMGVFLRYIFSQADPNPLLFYL 411

  Fly   961 ITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYRQDESLAREVDNVLQLEYDKVEILRTVFLRS 1025
            ..|..:..:..|......|:.:.||...|||. .:..|.|..|:::.|:...|    :|..|..:
Frog   412 CAEACQHLSPTDAFPLRKEVWNIFLDKSAPLR-IKIPEQLLNEIESRLRNSED----IRDKFSEA 471

  Fly  1026 RKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLRE-QIIDKYLMPNLHALIEDENG 1089
            :......|.||:::::.|||.|||::||..|  |.:.:.|..|| |:..|.:     |.:||
Frog   472 QGAMLPEIQEQIQDYRTKRTMGLGSLYGEND--LLDLEIDPHRERQVAAKQI-----AQLED--- 526

  Fly  1090 SPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPLV 1154
                                           |:.:....:|...:|                  .
Frog   527 -------------------------------ILSKYEKEISSPMAF------------------A 542

  Fly  1155 LRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKF 1219
            |..|               |...|.|..|    |....:....:|.|          ..:|...|
Frog   543 LNMY---------------VKYAGIHTQD----IRPASTSEKSQSIP----------DREKWLPF 578

  Fly  1220 MGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGL 1284
            ..|          |:|.|...:|       |:......|.|          |....|...:||
Frog   579 FPK----------NKKSSNAKKE-------DKSAEDKKRNP----------ILKYINKPKSTS-- 614

  Fly  1285 NTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVASTTPS------TSGSVAAGLSAFAELNALD 1343
                 ||:||...:...:.||...........:.....|.      ::||       |.|    |
Frog   615 -----QSTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPEHNLQRLSTGS-------FPE----D 663

  Fly  1344 TVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNR---NSRRKTSDPSLSSRPNDEQLDL 1405
            .:|.:..|           ..|.:.||||.|.|...|::|   |..|..||..:.:.....:|..
Frog   664 LLDSDGTR-----------LEVKLGRSESLKGREELKKSRKVDNVPRSRSDVDMDAAAEATRLHQ 717

  Fly  1406 GLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYC 1470
            ..|      ||.||| ||...::|:                 ||               :..:.|
Frog   718 SAS------SSASSL-SARSLDNPT-----------------PP---------------YTPKLC 743

  Fly  1471 QQDSFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDA 1535
            :: |.::...|..|....||....:.||         .|:.:.:.:..:|...|..:|...|...
Frog   744 RR-SIESPNLGFTGELLLSNLQEDDLGH---------LSDLDPEPDAQNWQHTVGRDVALHLLPK 798

  Fly  1536 EKKRQEIINEIYQTERNHVRTLKLLDRLFFLPLYESGLLSQDHLLLLFP--PALLSLREIHGAFE 1598
            |:.|||:|:|::.||.:|:|.|::||.:|:..:.:..||:.|.|.|:||  |.|:   |||.:..
Frog   799 ERARQEVIHELFVTEASHLRVLRVLDLIFYQRMRKENLLTPDELWLVFPNLPELI---EIHNSLA 860

  Fly  1599 QSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCEFAAQFCARQQIALEALKEKRNKDEMLQKLL 1663
            :|:|:.| |...|:..||||:...|||.:|..:...||:||:.|..|||.:|.|:.|:......:
Frog   861 ESMKRLR-EEGPVIKEIGDLMLLRFDGAAGEEVRMEAAKFCSYQISALELIKAKKRKETRFSLFM 924

  Fly  1664 KKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEAEAIQRAVESSKRILV 1728
            :::||:..||||:||||:.:.:|||||||||.||:.|.|    ...|.|.|.:.|..:..:.||.
Frog   925 QEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHT----ESGTQEHEKLYRCRDRCRDILK 985

  Fly  1729 EVNQAVKTAEDAHKLQNIQRKLDRSSYDKE------EFKKLDLTQHHLIHDGNLTIK-KNPSVQL 1786
            .||::||.||:.|:|...|::||.|..::.      |||.||||...:||:|.||.: ::.::.|
Frog   986 FVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAAEFKNLDLTTRRMIHEGPLTWRVRDKTIDL 1050

  Fly  1787 HGLLFENMIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTS 1851
            |.||.|:::|||.|||:|..||......:.|.:..||::.::: .|:|..|.||.:.|:|  .||
Frog  1051 HVLLLEDLLVLLQKQDEKLVLKCHSKTTTDTKQTFSPVIKLNS-VLVRSVATDKKALFII--CTS 1112

  Fly  1852 ----QMLELRAPSSSECKTWFKHFSDVAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLE 1912
                |:.||.|.:|||..||.    |:....:....:|                 |...||..
Frog  1113 DLGPQIYELVALTSSEKNTWM----DLLEEATNGAMRN-----------------PPGATKRK-- 1154

  Fly  1913 LSTDTVQPLAATATLTTTPLAPMLPIATV----TPAPATNNSNVSSLTG----VQLRNPQRDATA 1969
             |.|..:|.:|:..|....: |.|....|    :..|.:.:.......|    |.|..|:|
Frog  1155 -SQDINRPTSASMVLQRAHM-PKLTAEGVLRHNSEDPQSGDEGAGEFLGQKPDVVLEEPER---- 1213

  Fly  1970 SESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTGE 2034
             |.|.|                                  .||...||....|..|....|:.|
Frog  1214 -EDDED----------------------------------DEARTPTLLPIPSKEEDAGGGTRG- 1242

  Fly  2035 ERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAH-------TAEPVL 2092
                                  .::||:.                    |.|       .||..|
Frog  1243 ----------------------LDSSNHI--------------------PYHWPEVTEGLAESAL 1265

  Fly  2093 TPGEKLRRLDASIRNDLLEKQKIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTL 2157
            .....||||             |:.::.......||            ..||.:|
Frog  1266 DDVHNLRRL-------------ILKNMLHHGHSEYD------------SSSAPVA---------- 1295

  Fly  2158 TKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKKVAPSSSFSSSPPPLPPPNRQHAQAQAQIP 2222
                      .||.:::...:.:.||.|..:.:...|
Frog  1296 ----------EPEDDLTPTPSVIGGHGHPWDTMTDAV---------------------------- 1322

  Fly  2223 PSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPH-ERPTTPLAPFNTEPKTSQSVIDA 2286
                   |:.||.|:.. .|.:......:..|  ::.:.|| |||.:     ....:|:.|:::.
Frog  1323 -------LENLDADQTT-EEKETSVSRSESSR--SVLNFPHGERPGS-----RDGEETAWSLVER 1372

  Fly  2287 SKRQSTDAVPEGLLEQEPLEGD---------KTETKGEDNEVKTVPSDKLSESCNEER---QCVE 2339
            ..:.|  :|.:...||...|..         |.|..| :.:|.::|....|:|..:|.   ..|:
Frog  1373 YPQSS--SVQQNQSEQLHSEDHNNRSYRVVRKAEVVG-NYDVISLPDSSQSQSVLQEAGGGAIVD 1434

  Fly  2340 ADITKEVADPTTSKNEAAASVDELPSQSREIKTAEN-ASKSVADKKEDNEETIEEGVASTVDSST 2403
            .:.....|..|::|.:        |..|..:..::. :|.|..:.:|.|.       ..|..|..
Frog  1435 GNFFYVTASATSTKED--------PEVSESLTNSDRLSSPSFLESRESNH-------TPTYQSEF 1484

  Fly  2404 QTSPTESPKETDKLTGGSSSTCGPNRIQHASVLEPSVPCHALSSIVTILNEQISMLLPKINERDM 2468
            .|.|..| .|:|.|                  |...:|...:...|.|:...|..|..|::
Frog  1485 STPPPRS-TESDPL------------------LSDLLPPKLVIQDVDIIFRTIEHLTAKLH---- 1526

  Fly  2469 ERERLRKENQHLRELLSAL---------HDRQRVDE 2495
               ||::.....:|||.:|         |:|:...|
Frog  1527 ---RLKEVESAHQELLRSLGRSATEDHFHEREHTAE 1559

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	35/70 (50%)
		RGS_GEF_like	934..1055	CDD:188710	38/120 (32%)
		C1_1	1151..1202	CDD:278556	8/50 (16%)
		RhoGEF	1539..1731	CDD:238091	78/193 (40%)
		PH_RhoGEF	1770..1875	CDD:275411	41/109 (38%)
		DUF2413	2242..>2378	CDD:287302	28/149 (19%)
arhgef11	XP_017952119.2	PDZ_signaling	63..137	CDD:238492	35/69 (51%)
		RGS	357..501	CDD:413378	46/150 (31%)
		RhoGEF	805..989	CDD:214619	76/191 (40%)
		PH_PRG	1006..1143	CDD:275426	51/143 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	143	1.000	Domainoid score	I4613
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	391	1.000	Inparanoid score	I1946
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D163264at33208
OrthoFinder	1	1.000	-	-		FOG0001574
OrthoInspector	1	1.000	-	-		otm49122
Panther	1	1.100	-	-	LDO	PTHR45872
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2399
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	9.070