DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dark and apaf1

DIOPT Version :9

Sequence 1:	NP_725637.1	Gene:	Dark / 36914	FlyBaseID:	FBgn0263864	Length:	1440	Species:	Drosophila melanogaster
Sequence 2:	NP_571683.1	Gene:	apaf1 / 58131	ZFINID:	ZDB-GENE-000616-4	Length:	1261	Species:	Danio rerio

Alignment Length:	1473	Identity:	286/1473 - (19%)
Similarity:	540/1473 - (36%)	Gaps:	365/1473 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    33 QDMPKSILSKEEIDHIIMSKDAVSGTL----------RLFWTLLSKQEEMVQKFVEEVLRINYKF 87
            ||:..|.|....|...:::.|..:..|          .|..|||.|.......|...::|.:|..
Zfish    18 QDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGD 82

  Fly    88 LMS------PIKTEQRQ--------PSMMTRMY---IEQRDRLYNDNQVFAKYNVSRLQPYLKL- 134
            |.|      |:.:.:.:        ||:...:.   :.||..::          ||| .|.|.|
Zfish    83 LASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVF----------VSR-PPLLNLI 136

  Fly   135 RQALLELRPAKN-VLIDGVLGSGKTWVALDVCLSYKV--QCKMDFKIFWLNLKNCNSPETVLEML 196
            |:.|.:||.... |.:.|:.||||:.:|.:|.....:  :|..| .:.||::..|...:.::.|
Zfish   137 REMLYQLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPD-GVHWLSVGQCERADLLVRM- 199

  Fly   197 QKLLYQIDPNWTSRSDHSSNIKLRIHSIQAELRRLLKSKPYENCLLVLLNVQNAKAWNAFNLSCK 261
            |.|.::::.  ...||.|......:...:..||.|:..: :...||:|.:|.::.:..:|::.|:
Zfish   200 QSLCFRLEQ--CQSSDTSQRPPSTVEEAKERLRFLMLRR-FPRSLLILDDVWDSSSLRSFDIQCR 261

  Fly   262 ILLTTRFKQVTDFLSAATTTHISLDHHSMTLTPDEVKSL--LLKYLDCRPQDLPREVLTT----- 319
            :|||||.:.:||.:| .....:.:::..     ||.|:|  |..|::.:...||.:..:.
Zfish   262 VLLTTRNRALTDSVS-GVRYEVPVENGL-----DEEKALEILALYVNGKMHKLPEQARSIVSECK 320

  Fly   320 -NPRRLSIIAESIRDGLATWD---------NWKHV------NCDKLTTIIESSLNVLEPAEYRKM 368
             :|..:|:|...:|:....|.         .:|.:      :.:.|...:::||.||| ||::::
Zfish   321 GSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLE-AEHQEL 384

  Fly   369 FDRLSVFPPSAHIPTILLSLIW---FDVIKSDVMVVVNKLHKYSLVEKQPKESTISIPSIYLELK 430
            :..|||......:|..:||::|   .:.::..:...|||...:....::|..  ..:..:.|:..
Zfish   385 YRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYR--YYLHDLQLDFL 447

  Fly   431 VKLENEY--ALHRSIVDHY------NIPKTFDSDDLIPPYLDQYFYSHIGHHLKNIEHPERMTLF 487
            .:...:.  .||:.:|..|      ..|.:.|.|.|       |:|..|.:|:......:.  |:
Zfish   448 AEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSL-------YWYQFIPYHMAKAGLSKE--LY 503

  Fly   488 RMVF-LDFRFLEQKIRHDSTAWNASGSILNTLQQLKFYKPY--ICDNDPKYERLVNAILDFLPKI 549
            .::| ||  ::::|.|           |:.:...:..|..|  |.|.:....|:  ...:||...
Zfish   504 SLMFSLD--WVKEKAR-----------IMGSAHLINDYVEYGEILDKENSEVRV--QFQEFLSLN 553

  Fly   550 EENLICSKYTDLLRIALMAEDEA-IFEEAHKQVQRFDDRVWFTNHGRFHQHRQIINLGDNEGRHA 613
            ..:|....:.|::::||...|.: ::.:|..|.|:...|      |:.:.:  .:|....||...
Zfish   554 GHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASR------GQIYLN--WVNKNIEEGLSR 610

  Fly   614 VYLH------------NDFCLIA-LASGQILLTDVSLEGEDTYLLRDESDSSDILRMAVFNQQKH 665
            :.:|            .|...|| ..:.:.|....|..||.  ||..::...|:|..|.....:|
Zfish   611 LVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEK--LLELQAHEEDVLCCAFSPDDRH 673

  Fly   666 LITLHCNGSVKLWSLWPDCPGRRHSGGSKQQLVNSVVKRFIGSYANLKIVAFYLNEDAGLPEANI 730
            :.|...:..||||                                                  |:
Zfish   674 IATCASDRKVKLW--------------------------------------------------NV 688

  Fly   731 QLHVAFINGDVSILNWDEQDQEFKLSHVPVLKTMQSGIRCFVQVLKRYYVVCTSNCTLT---VWD 792
            :..|..              :||::.|...:...|     |....:|..:...||...|   :|:
Zfish   689 ERGVLI--------------REFEVEHEEQINHCQ-----FTNTGRRVLLATCSNDKFTNTRLWN 734

  Fly   793 LTNGSS-NTLELHVFNVENDTPLALDVFDERSKTATVLLIFKYS---VWRLNFLPGLSVSLQSEA 853
            ....:| ||:..|:..|.:......|::...|.:...|.:|:.|   .|:       |:.:.|
Zfish   735 PNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWK-------SIDVDS-- 790

  Fly   854 VQLPEG-----SFITCGKRSTDGRYLLLGTSEGLIVYDLKISDPVLRSNVSEHIECVDIYELFDP 913
             ..||.     :.:.|...|.||..::......:.|:|::.||.:|:...|.    :...:....
Zfish   791 -FFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLKLKTSR----LSTIQFCHA 850

  Fly   914 VYKYIVLCGAKGKQVVHVHTLRSVSGSNSHQNREIAWVH-------------SADEISVMTKACL 965
            .....:|..|.....|.:....| |...:..:..::|||             |:|:.::  :...
Zfish   851 CPNSSLLAVALSHYTVELWNFES-SKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTI--RLWE 912

  Fly   966 EPNVYLRSLMDMTRER--------TQLLAVDSKERIHLIKPAISRISEWSTITPTHAASNCKINA 1022
            ...|:..|.:.:.|:.        ..::|.||..|:.::..:...:    .:.....:|..:.:.
Zfish   913 TDRVHTSSAVALKRDTDVLSSHSDATIIAPDSSNRLQVLSGSTGAV----VLESEELSSRIRCSC 973

  Fly  1023 ISAFNDEQIFVGYVDGVIIDVIHDTALPQQFIEEPIDYLKQVSPNILVASAHSAQKTVIFQLEKI 1087
            ||. |...:.:|..||.:           |.||.|      .|...:..|.|:  |||
Zfish   974 ISR-NAAFVALGSEDGTV-----------QVIEVP------SSKASVKLSGHT--KTV------- 1011

  Fly  1088 DPLQPNDQWPLMM----DVSTKYASLQEGQYIILFSDHGVCHLDIANPSAFVKPKDSEEYIVGFD 1148
            ...|..|...:::    |.:.:....:.|:.::|..     |::.......:....|..
Zfish  1012 HHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQG-----HMEPVRKFHLLSSSSSPH------ 1065

  Fly  1149 LKNSLLFLAYENNIIDVFRLIFSCNQLRYEQICEEEIAQKAKISYLVATDDGTMLAMGFENGTLE 1213
                 ||....:..:.|:.|  :..|:..:.:|.|.......:|     .||.:.|....|.|.:
Zfish  1066 -----LFSWSFDGTVKVWDL--TRGQMLQDLVCHEGAVLSCDVS-----SDGRLFATTSANRTAK 1118

  Fly  1214 LFAVENRKVQLIYSIEEVHEHCIRQLLFSPCKLLLISCAE--QLCFWNVTHMRNNQL----EREQ 1272
            :::..:.|:..:.   |.|:.|:|...||.....|.:..:  ::..|:   |.:..|    .|:.
Zfish  1119 VWSSASWKMLFLL---EGHKDCVRSCRFSWDNKRLATGDDNGEIRLWS---MLDGALLKICPRDT 1177

  Fly  1273 KRRRSRRHKQHSVTQEDAVDAAPIAADIDVDVTFVAD-EFHPVNR------GTAELWRNKRGNAI 1330
            |...:..|                       ..:|.| .|.|.||      |..:.|..:.|
Zfish  1178 KDSMNSYH-----------------------AGWVTDLHFSPDNRVLVSTAGYIKWWSVESG--- 1216

  Fly  1331 RPELLACVKFVGNEARQFFTDAHFSHFYAIDDEGVYYHLQLLE 1373
              |.|.....:|...::......||.|..:|..|:.|.|:.||
Zfish  1217 --EALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLE 1257

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dark	NP_725637.1	NB-ARC	133..390	CDD:279299	69/283 (24%)
apaf1	NP_571683.1	DD	7..92	CDD:301326	17/73 (23%)
		NB-ARC	132..411	CDD:279299	71/290 (24%)
		P-loop_NTPase	150..297	CDD:304359	40/157 (25%)
		WD 1	615..654		8/40 (20%)
		WD40	616..912	CDD:238121	65/383 (17%)
		WD40 repeat	625..657	CDD:293791	9/33 (27%)
		WD40	641..1042	CDD:225201	90/519 (17%)
		WD 2	657..696		11/102 (11%)
		WD40 repeat	663..699	CDD:293791	12/99 (12%)
		WD 3	700..743		9/47 (19%)
		WD40 repeat	705..745	CDD:293791	10/44 (23%)
		WD 4	746..785		8/45 (18%)
		WD40 repeat	752..796	CDD:293791	10/53 (19%)
		WD 5	798..836		8/37 (22%)
		WD40 repeat	803..841	CDD:293791	9/37 (24%)
		WD 6	840..879		6/43 (14%)
		WD40	851..1256	CDD:225201	87/495 (18%)
		WD40	877..1212	CDD:238121	70/419 (17%)
		WD 7	882..921		6/40 (15%)
		WD40 repeat	887..911	CDD:293791	4/25 (16%)
		WD40 repeat	960..1005	CDD:293791	12/62 (19%)
		WD 8	964..1003		12/56 (21%)
		WD 9	1006..1045		8/47 (17%)
		WD40 repeat	1011..1046	CDD:293791	5/41 (12%)
		WD 10	1047..1088		7/58 (12%)
		WD 11	1091..1130		9/43 (21%)
		WD40 repeat	1096..1132	CDD:293791	7/43 (16%)
		WD 12	1133..1172		9/41 (22%)
		WD40 repeat	1138..1162	CDD:293791	4/23 (17%)
		WD 13	1184..1223		12/66 (18%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580604
Domainoid	1	1.000	54	1.000	Domainoid score	I11282
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D40665at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	6	5.810