Protein Alignment Fen1 and ERCC5

DIOPT Version :10

• Sequence 1: NP_523765.1
• Gene: Fen1 / 36887
• FlyBaseID: FBgn0025832
• Length: 385
• Species: Drosophila melanogaster
• Sequence 2: NP_000114.3
• Gene: ERCC5 / 2073
• HGNCID: 3437
• Length: 1186
• Species: Homo sapiens
• Alignment Length: 346
• Identity: 95/346 - (27%)
• Similarity: 144/346 - (41%)
• Gaps: 71/346 - (20%)

  Fly    86 DGKPPDLKSGELAKRAER------REEAEKALKAATDAGDDAGIEKF------------------ 126
            :|:.|......|...:||      .:||||..:.:.....|..:|:.
Human   690 EGEAPAESESLLRDNSERDDVDGEPQEAEKDAEDSLHEWQDINLEELETLESNLLAQQNSLKAQK 754

  Fly   127 ---NRRLVRVTKEHAKEAKELLTLMGVPYVDAPCEAEAQCAALVKAGKVYATATEDMDALTFGST 188
               .|....||.:...|::|||.|.|:||:.||.|||||||.|....:...|.|:|.|...||:.
Human   755 QQQERIAATVTGQMFLESQELLRLFGIPYIQAPMEAEAQCAILDLTDQTSGTITDDSDIWLFGAR 819

  Fly   189 KLLRYLTYSEARKMPVKEFSYDKLLEGLAINNREFIDLCILLGCDYCESIKGIGPKRAIELINTY 253
            .:  |..:....|. |:.:.|......|.::..:.|:|..|||.||.|.|..:|...|:|::|.:
Human   820 HV--YRNFFNKNKF-VEYYQYVDFHNQLGLDRNKLINLAYLLGSDYTEGIPTVGCVTAMEILNEF 881

  Fly   254 --RDIETIL------DNLDSSKYTVPENWNYKVAREL--------FIEPEVADA-------DSI- 294
              ..:|.:|      .....:....|...:.||.::|        |..|.||:|       ||.
Human   882 PGHGLEPLLKFSEWWHEAQKNPKIRPNPHDTKVKKKLRTLQLTPGFPNPAVAEAYLKPVVDDSKG 946

  Fly   295 DLKWVEPDEEGLVKFLCGDRQFNEERVRNGAKKLMKSKQ---AQTQVRLDSFFKTLPSTPNATNA 356
            ...|.:||.:.:.:| | .|.|...|.:.........||   .|||:|:||||:          .
Human   947 SFLWGKPDLDKIREF-C-QRYFGWNRTKTDESLFPVLKQLDAQQTQLRIDSFFR----------L 999

  Fly   357 AKRKAEEAK--KSANNKKAKT 375
            |:::.|:||  ||....:|.|
Human  1000 AQQEKEDAKRIKSQRLNRAVT 1020

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Fen1	NP_523765.1	PTZ00217	1..363	CDD:240317	88/330 (27%)
ERCC5	NP_000114.3	rad2	1..1029	CDD:273166	95/346 (27%)
		N-domain. /evidence=ECO:0000305|PubMed:32522879	1..78
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000269|PubMed:32821917, ECO:0000312|PDB:6TUW, ECO:0007744|PDB:6TUX	31..67
		Spacer region. /evidence=ECO:0000269|PubMed:16246722	79..785		22/94 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	306..342
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	354..385
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	404..473
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	510..533
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	667..724		9/33 (27%)
		I-domain. /evidence=ECO:0000305|PubMed:32522879	786..881		34/97 (35%)
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000269|PubMed:32821917, ECO:0000312|PDB:6TUW, ECO:0007744|PDB:6TUX	820..836		3/18 (17%)
		DNA-binding, H2TH (helix-2turn-helix) motif which binds double-stranded DNA. /evidence=ECO:0000269|PubMed:32821917, ECO:0000312|PDB:6TUW, ECO:0007744|PDB:6TUX	848..880		12/31 (39%)
		DNA-binding, may bind double-stranded DNA. /evidence=ECO:0000269|PubMed:32821917, ECO:0000312|PDB:6TUW, ECO:0007744|PDB:6TUX	912..918		2/5 (40%)
		Interaction with PCNA. /evidence=ECO:0000269|PubMed:9305916	981..1009		13/37 (35%)
		Interaction with ERCC6/CSB. /evidence=ECO:0000269|PubMed:16246722	1011..1186		4/10 (40%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1056..1081
		Nuclear localization signal 1. /evidence=ECO:0000305|PubMed:26812207	1057..1074
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1095..1186
		Nuclear localization signal 2. /evidence=ECO:0000305|PubMed:26812207	1169..1186