DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-104 and kif1b

DIOPT Version :9

Sequence 1:	NP_611155.3	Gene:	unc-104 / 36876	FlyBaseID:	FBgn0267002	Length:	1739	Species:	Drosophila melanogaster
Sequence 2:	XP_009295087.1	Gene:	kif1b / 369190	ZFINID:	ZDB-GENE-030820-1	Length:	1924	Species:	Danio rerio

Alignment Length:	1980	Identity:	1015/1980 - (51%)
Similarity:	1268/1980 - (64%)	Gaps:	333/1980 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 SSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSH-DHHDA 65
            :|||||||||||||||..:||||||:|.|.:|.|.|||.|...    |.|:|||||||| ...|.
Zfish     4 ASVKVAVRVRPFNSRETGKESKCIIQMQGNSTTILNPKNPKEP----KTFSFDYSYWSHTSPDDP 64

  Fly    66 DFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRI 130
            .|::|:.||.|||:|||||:|:||||||||||||||||||||||:|||.||||||.:|::||.:|
Zfish    65 SFASQNQVYNDIGKEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEEGQEGIIPQLCEELFEKI 129

  Fly   131 QDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDL 195
            .|...:::.|||||:||||||||||||||||||||||||||||||||||||||||||.|.||.||
Zfish   130 NDNNNEEISYSVEVAYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADL 194

  Fly   196 IDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGA 260
            :|.|||||||||||||||||||||||||.||||::|..|:|:|||||||||||||||||||||||
Zfish   195 MDAGNKARTVAATNMNETSSRSHAVFTIVFTQRKYDSETDLSTEKVSKISLVDLAGSERADSTGA 259

  Fly   261 KGTRLKEGANINKSLTTLGKVISALAEV---ASKKKNTKKADFIPYRDSALTWLLRENLGGNSKT 322
            ||||||||||||||||||||||||||||   .||.|..||.||||||||.|||||||||||||:|
Zfish   260 KGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRT 324

  Fly   323 AMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEG--- 384
            ||:||:||||||:||||||||||||||||.|.||:|||.||||:||||:|:.:|::||:|:|
Zfish   325 AMVAALSPADINFDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVSRLKELLRAQGLGD 389

  Fly   385 ---IEVQEEDELTK----------STVIKSPTKS------------------------------- 405
               ||...:|.|..          .|::.|.|::
Zfish   390 ILDIEPMGDDCLGSGSKCDDINDVKTLLSSQTEAMLYRRKAPMGCLTASPSSGSLCSQAGLQSVS 454

  Fly   406 --RNRNGSTT--EMAVDQLQASEKLIAELNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITV 466
              :.|..||.  |.|:::|:.|||:||||||||||||::||.||::|||:.||||||::|||.|:
Zfish   455 SIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTL 519

  Fly   467 ------------------------------------------GVFSPKKTPHLVNLNEDPNLSEC 489
                                                      ||||||||||||||||||.:|||
Zfish   520 GVFSPKKFGPDRPTSLSIEDFSVYSSNQLFVERPCEVYTDFNGVFSPKKTPHLVNLNEDPLMSEC 584

  Fly   490 LLYYIKEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKNST-----VTLLPHKDAIIYVNGRK 549
            ||||||:|:||:|..:|...|||.|||:||.:|||.|.::.:.     |||.|.:.:..||||::
Zfish   585 LLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNANGHVIVTLEPCEGSETYVNGKR 649

  Fly   550 LVEPEVLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLE 614
            :.....|::|:|:|:||||||||.:|||||..|:|..:   ||..||     .|||.|||.||||
Zfish   650 VNSAVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPS---AETPVE-----PVDWTFAQRELLE 706

  Fly   615 KQGIDLKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSP 679
            |||||:|.||:|||..:|..||:||.:|||..|:||..||:::..||||||.:|:     ::.:|
Zfish   707 KQGIDMKQEMEKRLTEMEILYKKEKEEADQLLEQQRLVYESKLQELQKQVETRSL-----AAETP 766

  Fly   680 EDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKK 744
            ::..:||:....|     ||..:..||.||||||||||||||||.|||||::|||||||||||||
Zfish   767 DEEEEEEEEDEVP-----WTQHKYELAQWAFRKWRYHQFTSLRDQLWGNAVYLKEANAISVELKK 826

  Fly   745 KVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLR 809
            ||||||.||||||||||||||    .|...|.|..|.|..:|:|||||.|.||||||:||||||:
Zfish   827 KVQFQFVLLTDTLYSPLPPEL----LPPEPEKERDARPFPRTVVAVEVQDLKNGATHYWSLEKLK 887

  Fly   810 YRLELMRQIYNVESPPSSMLFDTSGMEALSG---------WI----SPPSQHPGQQAQLLPLEPP 861
            .||:.||::|:.....:|......|..||:|         |.    |.|..|.....:|....|.
Zfish   888 QRLDQMREMYDRAGEMASSNQGDDGEGALTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPS 952

  Fly   862 VESERGRLTLANLIPSRQRLELMREMYHNEAEMSPTSPDYNVES-----LTGGDPFYDRFPWFRM 921
            .........:..|...||  ..|.:...:||.:..||.|...|.     ..|.||||||.|||.:
Zfish   953 PTFSTTDSEITELADERQ--SEMEDFMDDEAFVDDTSSDAGTEEGSDIFSDGQDPFYDRSPWFIL 1015

  Fly   922 VGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPV-LDEESIDFNNGVKQS--ARLVFN 983
            |||:|:|||||||||||||:||:|.|:|||||:||:.||.: .|||:.|:.:||:||  |::.|:
Zfish  1016 VGRAFVYLSNLLYPVPLVHRVAVVTEKGDVRGFLRVGVQAIAADEEAPDYGSGVRQSGTAKISFD 1080

  Fly   984 EDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGIDSNSASECHENSEEP-------- 1040
            .:..|       :.|.....:...||  .||||..|: |:|    .:||....|||.
Zfish  1081 NEYFK-------KNDFTSVAMTRSGL--SLEELRIVE-GQG----QSSEVITPSEEMNRINEMDL 1131

  Fly  1041 -------------GEHLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHRHEEAFSTEPVKNSA 1092
                         .|||:||..||||||||||:||.||||||||||||||||:|||||||:||:.
Zfish  1132 KLGNVDTKLGDGLAEHLEVGSIFTFRVTVLQASGIPAEYADIFCQFNFLHRHDEAFSTEPLKNTG 1196

  Fly  1093 SGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDFVS-RPPPRRMLPPS 1156
            .||||||||||||:|.||:|||||:||:||:|::|||||.||||... ||.|| ..|.|:..||.
Zfish  1197 RGAPLGFYHVQNISVEVTESFIEYIKTKPIVFEVFGHYQQHPLHIHG-QDVVSPSQPSRKYYPPP 1260

  Fly  1157 IPISQPVRSPK----------------------------------------------FGPLPCAP 1175
            :|:|:|..:.|                                              |.|:|..|
Zfish  1261 MPLSKPDHARKIELIRYLMSGYVHGQVLDTLSEHANALASTAVASLEEGAYELCHFPFLPIPNCP 1325

  Fly  1176 TSTV---------------------LAKHDVLVWFEICELAPNGEYVPSVVEHSDDLPCRGLFLL 1219
            ...|                     ::|:|:|.||||.||.|.|||:|:||:|:..|||.|.:||
Zfish  1326 VFRVPRHDVPATKLNTITKSNLGQCVSKYDLLAWFEISELEPTGEYIPAVVDHTGGLPCHGTYLL 1390

  Fly  1220 HQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDACVLSLGLFPGEALE---- 1280
            ||||||||.:|::||..:|:.|||:.|||||||||.|| .|:...|| ||||.:...:.|:
Zfish  1391 HQGIQRRITVTLIHEKGSELHWKDVRELVVGRIRNKPE-VDDSAADA-VLSLNIISAKNLKSSHN 1453

  Fly  1281 -----VPGD-DRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENCARPAIITKDLS 1339
                 :..| .|:||||||.||||||||.|||||:..||.||:||||||||::|.:|||||||:.
Zfish  1454 SSSLCIDSDISRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAIITKDIC 1518

  Fly  1340 MVIYGRDAR-TGPRSLKHLF-SGQYRNPEANRLTGVYELALRRASEAGSPGVQRRQRRVLDTSST 1402
            ||.|.|||: :.||||::|| ||..:.|:.|::||||||:|.:.::.||||:|||:|:|||||..
Zfish  1519 MVFYSRDAKISPPRSLRNLFGSGYSKTPDCNKVTGVYELSLCKIADTGSPGMQRRRRKVLDTSVA 1583

  Fly  1403 YVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVC 1467
            ||||||||.||||||||||.:|||||:|:.:|.||.:.||||||||:||  ..|.|.:.:|....
Zfish  1584 YVRGEENLAGWRPRGDSLILEHQWELDKMEQLHEVEKTRHLLLLREKLG--ETPPPKSLSESLSP 1646

  Fly  1468 NLA------ARAATSPVHMVIPQSPQTPVK-------DPQQIIPEREYNQREQDLMLKCLKLVQG 1519
            :|:      :.:.:|.:.....:|..||.:       |.:.::      .||::|..|||:|:..
Zfish  1647 SLSSGTLSTSTSISSQISSTTFESAITPSESSGYDSTDIESLV------DREKELATKCLRLLTH 1705

  Fly  1520 RYTKSEANDTQTQ------SDVSPSDEGCADMTVSCISSNSMENNKFVIRRRLCSPDRADAPNGW 1578
            .: .||.|.....      ||:||...   |.:||..||.::..:.      .| |..:|:...
Zfish  1706 TF-NSEYNQMCNSISDCKFSDISPMGR---DPSVSSFSSATLTPSS------TC-PSLSDSRTP- 1758

  Fly  1579 EAPAPATQPA---------LP---------------LRLYVPELEEIRVSPVVARKGLLNVLEHG 1619
            ||.:.||.|:         :|               |.| ||.:||:|...||::||:||.:|..
Zfish  1759 EANSRATSPSGSDYENFPMVPILETSYLARAGKHEFLNL-VPNIEEMRPGSVVSKKGILNFMEPR 1822

  Fly  1620 GSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVVTKHRGYLL 1684
            .:.|.|.:|:|||||||||.::||||||.||||:||.||.||||.||:|.|:||::.|||||.||
Zfish  1823 SNTWVKHFVVVRRPYVFIYNNDKDPVERGVLNLSTAQVEYSEDQQAMLKTPHTFAMCTKHRGILL 1887

  Fly  1685 QTLGDKEVHDWLYAINPLLAGQIKSRLARR 1714
            |...||:::|||||.||||||.|:|:||||
Zfish  1888 QANNDKDMNDWLYAFNPLLAGTIRSKLARR 1917

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-104	NP_611155.3	KISc_KIF1A_KIF1B	2..358	CDD:276816	281/359 (78%)
		KISc	3..358	CDD:214526	281/358 (78%)
		Kinesin_assoc	355..498	CDD:292801	91/235 (39%)
		FHA	475..574	CDD:238017	56/103 (54%)
		KIF1B	878..923	CDD:289208	19/49 (39%)
		DUF3694	1246..1347	CDD:289256	64/110 (58%)
		PH_KIFIA_KIFIB	1602..1704	CDD:269939	62/101 (61%)
		PH	1607..1700	CDD:278594	57/92 (62%)
kif1b	XP_009295087.1	KISc_KIF1A_KIF1B	4..360	CDD:276816	281/359 (78%)
		KISc	5..360	CDD:214526	281/358 (78%)
		Kinesin_assoc	357..593	CDD:292801	91/235 (39%)
		FHA	570..674	CDD:238017	56/103 (54%)
		FHA	<580..672	CDD:224630	44/91 (48%)
		GBP_C	<677..751	CDD:303769	43/81 (53%)
		KIF1B	886..934	CDD:289208	12/47 (26%)
		DUF3694	1417..1526	CDD:289256	64/110 (58%)
		PH_KIFIA_KIFIB	1805..1907	CDD:269939	62/101 (61%)
		PH	1810..1903	CDD:278594	57/92 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	537	1.000	Domainoid score	I262
eggNOG	1	0.900	-	-		E2759_KOG0245
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1801	1.000	Inparanoid score	I63
OMA	1	1.010	-	-		QHG52590
OrthoDB	1	1.010	-	-		D131864at33208
OrthoFinder	1	1.000	-	-		FOG0001470
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100247
Panther	1	1.100	-	-	LDO	PTHR24115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2883
SonicParanoid	1	1.000	-	-		X1999
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.910