Protein Alignment unc-104 and KIF4A

DIOPT Version :10

• Sequence 1: NP_611155.3
• Gene: unc-104 / 36876
• FlyBaseID: FBgn0267002
• Length: 1739
• Species: Drosophila melanogaster
• Sequence 2: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Alignment Length: 942
• Identity: 277/942 - (29%)
• Similarity: 424/942 - (45%)
• Gaps: 227/942 - (24%)

  Fly     4 VKVAVRVRPFNSREIARESK-CIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADF 67
            |:||:|.||...:||:...: |:..:.|      .|:|...|.   |.|.:|:.:        |.
Human    10 VRVALRCRPLVPKEISEGCQMCLSFVPG------EPQVVVGTD---KSFTYDFVF--------DP 57

  Fly    68 ST-QSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQE-----GIIPMICKDL 126
            || |..|:......:::..|.|||..:.||||||:||:|:|.|....:||     |:||.:.:.|
Human    58 STEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLL 122

  Fly   127 FTRIQDTETDDLKYSVEVSYMEIYCERVRDLLNP-KNKGNLRVREHPLLGPYVEDLSKLAVTDYQ 190
            |..|.  :..|.:::::|||:|||.|.:.|||.| :.|..:.:||.|..|..:..|::..|....
Human   123 FKEID--KKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVAL 185

  Fly   191 DIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERA 255
            |....:::||.:||||:|.||..||||||:|||...||:   .::..:...||:.|||||||||.
Human   186 DTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRK---KSDKNSSFRSKLHLVDLAGSERQ 247

  Fly   256 DSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKKADFIPYRDSALTWLLRENLGGNS 320
            ..|.|:|.|||||.|||:.|..||.|||||.:       .||..|:|||||.||.||:::|||||
Human   248 KKTKAEGDRLKEGININRGLLCLGNVISALGD-------DKKGGFVPYRDSKLTRLLQDSLGGNS 305

  Fly   321 KTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRD-LLKAEG 384
            .|.|||.:||||.|.:|||:||||||||::|..|.:||.|.....:..||:::|:|:. ||:|.|
Human   306 HTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHG 370

  Fly   385 ------IEVQEEDEL------TKSTVIKSPTKSRNRN---GSTTEMAVDQL----QASEKLIAEL 430
                  |.|:..:.|      .:|.|.::...||..:   |.|.:| ::::    ||:||:.|:|
Human   371 GTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQM-LERIILTEQANEKMNAKL 434

  Fly   431 N----------------ETWE-EKLKRTEEIRVQREAVFAEMG---VAVKEDGITVGV------- 468
            .                ||.| ::||...||....:.:..::.   ||.....|...|
Human   435 EELRQHAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDTAVEQEAQVE 499

  Fly   469 FSPK--------KTPH----------LVNLNEDPNLSECLLYYIKEGLTRLGTHEANVPQDIQLS 515
            .||:        .|.|          ||.||:        ...:||.|.|..|...:..|.||..
Human   500 TSPETSRSSDAFTTQHALRQAQMSKELVELNK--------ALALKEALARKMTQNDSQLQPIQYQ 556

  Fly   516 GSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPE-QAR 579
            ....:|     |.:...:.|...|:.::      |......|..::..|.:....|....| |..
Human   557 YQDNIK-----ELELEVINLQKEKEELV------LELQTAKKDANQAKLSERRRKRLQELEGQIA 610

  Fly   580 ELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGIDLKAEMK--------------KRLDN 630
            :|:.|:..:::.....|.|:......| .:..:::.|.:.|..:||              |.:..
Human   611 DLKKKLNEQSKLLKLKESTERTVSKLN-QEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQ 674

  Fly   631 LEEQYKR---EKLQADQQFEEQ-----RKTYEARI------DALQKQ---VEEQSMTMSMYSSYS 678
            |:|:.::   |.|:.::.|::|     |||.||..      ||||||   .:::..|.|
Human   675 LKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREVADKRKETQS------ 733

  Fly   679 PEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLW-GNAIFLKEANAISVEL 742
                                               |..:.|:.|...| ||.|   |....:.|.
Human   734 -----------------------------------RGMEGTAARVKNWLGNEI---EVMVSTEEA 760

  Fly   743 KKKVQFQFTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSK--------TLVAVEVTDTKNGA 799
            |:.:.   .||.|...      ||..||.:.::.|.|..|..|        |.|..:|:::::..
Human   761 KRHLN---DLLEDRKI------LAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSI 816

  Fly   800 THHWSLEKLRYRLE--------LMRQIYNVES 823
            |.  .:|.|...:|        |.:::.:.||
Human   817 TK--QIESLETEMEFRSAQIADLQQKLLDAES 846

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
unc-104	NP_611155.3	KISc_KIF1A_KIF1B	2..358	CDD:276816	153/361 (42%)
		Kinesin_assoc	355..498	CDD:465047	48/207 (23%)
		FHA_KIF1	474..574	CDD:438757	22/109 (20%)
		YhaN	<575..>666	CDD:443752	29/122 (24%)
		KIF1B	878..924	CDD:463574
		DUF3694	1199..1347	CDD:463599
		PH_KIFIA_KIFIB	1602..1704	CDD:269939
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	151/355 (43%)
		PRK11281	383..>646	CDD:481316	56/283 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		3/18 (17%)
		Smc	<517..>1033	CDD:440809	82/405 (20%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232		51/239 (21%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798		1/4 (25%)
		Globular	1000..1232
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142