DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-104 and Kif1a

DIOPT Version :9

Sequence 1:	NP_611155.3	Gene:	unc-104 / 36876	FlyBaseID:	FBgn0267002	Length:	1739	Species:	Drosophila melanogaster
Sequence 2:	XP_006529218.1	Gene:	Kif1a / 16560	MGIID:	108391	Length:	1800	Species:	Mus musculus

Alignment Length:	1858	Identity:	1002/1858 - (53%)
Similarity:	1260/1858 - (67%)	Gaps:	213/1858 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 SSVKVAVRVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSH-DHHDA 65
            :|||||||||||||||::|:|||||:|:|:||.|.|||.|..|.   |.|:|||||||| ...|.
Mouse     4 ASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETP---KSFSFDYSYWSHTSPEDI 65

  Fly    66 DFSTQSMVYKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGRQEEQQEGIIPMICKDLFTRI 130
            ::::|..||:|||||||||:|:||||||||||||||||||||||:||:.|:||||.:|:|||:||
Mouse    66 NYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRI 130

  Fly   131 QDTETDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTDYQDIHDL 195
            .||..|::.||||||||||||||||||||||||||||||||||||||||||||||||.|.||.||
Mouse   131 NDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDL 195

  Fly   196 IDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMTNLTTEKVSKISLVDLAGSERADSTGA 260
            :|.|||||||||||||||||||||||.|.|||:|||..||:||||||||||||||||||||||||
Mouse   196 MDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGA 260

  Fly   261 KGTRLKEGANINKSLTTLGKVISALAEVAS---KKKNTKKADFIPYRDSALTWLLRENLGGNSKT 322
            |||||||||||||||||||||||||||:.|   |.|..||.||||||||.|||||||||||||:|
Mouse   261 KGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRT 325

  Fly   323 AMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRDLLKAEGI-- 385
            ||:||:|||||||||||||||||||||||.|.|::|||.|.|||||||:|:.:|||||.|:|:
Mouse   326 AMVAALSPADINYDETLSTLRYADRAKQIRCNAIINEDPNNKLIRELKDEVTRLRDLLYAQGLGD 390

  Fly   386 -----EVQEEDELTKSTVIKSPTKSRNRNGST----------------TEMAVDQLQASEKLIAE 429
                 .|....:||.:.|..||:.|.:...|.                :|.|:::|:.:||:|||
Mouse   391 ITDTNTVPGGPKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAE 455

  Fly   430 LNETWEEKLKRTEEIRVQREAVFAEMGVAVKEDGITVGVFSPKKTPHLVNLNEDPNLSECLLYYI 494
            |||||||||:|||.||::|||:.||||||::|||.|:||||||||||||||||||.:||||||||
Mouse   456 LNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYI 520

  Fly   495 KEGLTRLGTHEANVPQDIQLSGSHILKEHCTFENKN-----STVTLLPHKDAIIYVNGRKLVEPE 554
            |:|:||:|..:|...|||.|||..|.:|||.|.:.:     :.|||.|.:.|..||||:|:.||.
Mouse   521 KDGVTRVGREDAERRQDIVLSGHFIKEEHCIFRSDSRGGGEAVVTLEPCEGADTYVNGKKVTEPS 585

  Fly   555 VLKTGSRVILGKNHVFRFTNPEQARELRDKIETENEAENEVEKTDTQQVDWNFAQCELLEKQGID 619
            :|::|:|:|:||:|||||.:|||||:.|::        ....:|..:.|||.|||.|||||||||
Mouse   586 ILRSGNRIIMGKSHVFRFNHPEQARQERER--------TPCAETPAEPVDWAFAQRELLEKQGID 642

  Fly   620 LKAEMKKRLDNLEEQYKREKLQADQQFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQ 684
            :|.||::||..||:||:||:.:|....|:||..||::::|||||::         |.|.||...:
Mouse   643 MKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMD---------SRYYPEVNEE 698

  Fly   685 EEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQ 749
            ||:    |..|..||.||..||.||||||:::|||||||.|||||||||||||||||||||||||
Mouse   699 EEE----PEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQ 759

  Fly   750 FTLLTDTLYSPLPPELASTVAPVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRYRLEL 814
            |.||||||||||||:|....|...:|..    |..:|:|||||.|.||||||:|:|||||.||:|
Mouse   760 FVLLTDTLYSPLPPDLLPPEAAKDRETR----PFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDL 820

  Fly   815 MRQIYN-VESPPSSMLFDTS-------------------GMEALSGWISPPSQHPGQQAQLLPLE 859
            ||::|: ....|||::.|..                   |...:||..|.|..:.....::..|.
Mouse   821 MREMYDRAAEVPSSVVEDCDNVVTGGDPFYDRFPWFRLVGSSVISGCNSYPLLNTCMSERMAALT 885

  Fly   860 P-------------PVESERGRLTLANLIPSRQRLELMREMYHNEAEM-SPTSPDYNVESLTGGD 910
            |             |.|.:.        :...:..|...|....|.:: ....|::.:  ..|.|
Mouse   886 PSPTFSSPDSDTTEPAEEQS--------VGEEEEEEEEEEEEEEEEDLEDDVFPEHTL--CDGQD 940

  Fly   911 PFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQPV-LDEESIDFNNGV 974
            |||||.|.|.:|||:|:|||||||||||||:||||:|:|:|:|:||:|||.: .|||:.|:.:||
Mouse   941 PFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGV 1005

  Fly   975 KQS--ARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVD-SGRGIDSN-SASECHE 1035
            :||  |::.| :|....|::  :|...|.....:|   :..|||..|: .|:|.|:. ||.|.:.
Mouse  1006 RQSGTAKISF-DDQHFEKFQ--SESCPVVGMSRSG---TSQEELRIVEGQGQGADAGPSADEVNN 1064

  Fly  1036 N--SEEPGE--------------------HLQVGKEFTFRVTVLQATGIGAEYADIFCQFNFLHR 1078
            |  |..|.|                    ||::|..||||||||||:.|.||||||||||||:||
Mouse  1065 NTCSAVPPEGLMDSPEKAALDGPLDTALDHLRLGSTFTFRVTVLQASSISAEYADIFCQFNFIHR 1129

  Fly  1079 HEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKDAKQDF 1143
            |:|||||||:||:..|.||||||||||.|.||||||||:|:|||:|::|||||.||.....|...
Mouse  1130 HDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVL 1194

  Fly  1144 VSRPPPRRMLPPSIPISQPVRSPKF------GPLPCAPTSTVLAKHDVLVWFEICELAPNGEYVP 1202
            ....|.||..|..:|:|:||.:.|.      .|.||.      .|:|:||:||||||..||:|:|
Mouse  1195 SPLRPSRRHFPRVMPLSKPVPATKLSTMTRPSPGPCH------CKYDLLVYFEICELEANGDYIP 1253

  Fly  1203 SVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTTEVKWKDINELVVGRIRNTPESSDEQDEDAC 1267
            :||:|...:||.|.|||||||||||.:|::||..:.::||::.|||||||||||| :||...|..
Mouse  1254 AVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPE-TDEALIDPN 1317

  Fly  1268 VLSLGLFPGEALEVPGDD---------RSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYL 1323
            :|||.:.....:....||         |:||:||||||||:|||.|||||:...|.||:|||||:
Mouse  1318 ILSLNILSSGYVHPAQDDRQFLDSDIPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYI 1382

  Fly  1324 ELENCARPAIITKDLSMVIYGRDAR-TGPRSLKHLF-SGQYRNPEANRLTGVYELALRRASEAGS 1386
            |:|||.:||:||||..||.|.|||: ...||:::|| ||..|..|.||:||||||:|...::|||
Mouse  1383 EMENCTQPAVITKDFCMVFYSRDAKLPASRSIRNLFGSGSLRATEGNRVTGVYELSLCHVADAGS 1447

  Fly  1387 PGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLG 1451
            ||:|||:|||||||..||||||||.|||||.||||.|||||||||:.|:||.:.||.|||||:|.
Mouse  1448 PGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLE 1512

  Fly  1452 MDTNPNP---TTKTEKDVCNLAARAATSPVHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKC 1513
            ....|.|   :..:.:|..:.::..|:||:.   .:...:|::.|         |:|:::|.:||
Mouse  1513 TTQRPGPEVLSPASSEDSESRSSSGASSPLS---AEGQPSPLEAP---------NERQRELAVKC 1565

  Fly  1514 LKLV----QGRYTKSEANDTQTQS-----------DVSPSDEGCADMTVSCISSNSMENNKFVIR 1563
            |:|:    ...||.|....:.::|           |.|.|..|.|.:|.|....:.:|.......
Mouse  1566 LRLLMHTFNREYTHSHVCISASESKLSEMSVTLMRDPSMSPLGAATLTPSSTCPSLIEGRYGATD 1630

  Fly  1564 RRLCSPDRADAPNGWEAPAPATQPAL-----------------PLRLYVPELEEIRVSPVVARKG 1611
            .|  :|.....|   .:|.|...|.|                 |.||.||:::||||||:|::||
Mouse  1631 VR--TPQPCSRP---ASPEPELLPELDSKKTPSPVRATETEKEPQRLLVPDIQEIRVSPIVSKKG 1690

  Fly  1612 LLNVLEHGGSGWKKRWVIVRRPYVFIYRSEKDPVERAVLNLATAHVECSEDQAAMVKIPNTFSVV 1676
            .|:.||...:||.||:|:|||||.::|.|:||.|||.||||:||.||.||||.||:|.||||:|.
Mouse  1691 YLHFLEPHTAGWAKRFVVVRRPYAYMYNSDKDTVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVC 1755

  Fly  1677 TKHRGYLLQTLGDKEVHDWLYAINPLLAGQIKSRLARR 1714
            |:|||.|||...||::||||||.||||||.|:|:|:||
Mouse  1756 TEHRGILLQANSDKDMHDWLYAFNPLLAGTIRSKLSRR 1793

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-104	NP_611155.3	KISc_KIF1A_KIF1B	2..358	CDD:276816	286/359 (80%)
		KISc	3..358	CDD:214526	286/358 (80%)
		Kinesin_assoc	355..498	CDD:292801	90/165 (55%)
		FHA	475..574	CDD:238017	58/103 (56%)
		KIF1B	878..923	CDD:289208	13/45 (29%)
		DUF3694	1246..1347	CDD:289256	61/109 (56%)
		PH_KIFIA_KIFIB	1602..1704	CDD:269939	65/101 (64%)
		PH	1607..1700	CDD:278594	58/92 (63%)
Kif1a	XP_006529218.1	KISc_KIF1A_KIF1B	4..361	CDD:276816	286/359 (80%)
		Kinesin_assoc	358..524	CDD:374417	90/165 (55%)
		FHA	501..605	CDD:238017	58/103 (56%)
		DUF572	<607..>685	CDD:367968	40/85 (47%)
		KIF1B	814..861	CDD:372100	12/46 (26%)
		DUF3694	1250..1406	CDD:372133	85/156 (54%)
		PH_KIFIA_KIFIB	1681..1783	CDD:269939	65/101 (64%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	556	1.000	Domainoid score	I245
eggNOG	1	0.900	-	-		E2759_KOG0245
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H99729
Inparanoid	1	1.050	1803	1.000	Inparanoid score	I64
Isobase	1	0.950	-	0	Normalized mean entropy	S2016
OMA	1	1.010	-	-		QHG52590
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001470
OrthoInspector	1	1.000	-	-		oto92225
orthoMCL	1	0.900	-	-		OOG6_100247
Panther	1	1.100	-	-	O	PTHR24115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2883
SonicParanoid	1	1.000	-	-		X1999
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.810