Protein Alignment Sema2a and sema4aa

DIOPT Version :10

• Sequence 1: NP_477507.1
• Gene: Sema2a / 36846
• FlyBaseID: FBgn0011260
• Length: 724
• Species: Drosophila melanogaster
• Sequence 2: XP_009290884.1
• Gene: sema4aa / 798168
• ZFINID: ZDB-GENE-110411-86
• Length: 739
• Species: Danio rerio
• Alignment Length: 693
• Identity: 187/693 - (26%)
• Similarity: 313/693 - (45%)
• Gaps: 110/693 - (15%)

  Fly    70 TFHMNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVR 134
            |..::.|.|||:|||.|.:|.:::.. |.|...:|.:...|:.|...:|....|   .||.|.:.
Zfish    53 TLLLSTDEDTLFVGARDALFSLDVSQ-SDSITLKDELKWTPSSDQTKTCRVASK---LDCGNFIS 113

  Fly   135 VIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIAK--CPYDPLDNSTAIYVE 197
            ::|..: ...||.|||||:.|:..:|       |.|.....:....||  ||:.....:|||.|:
Zfish   114 ILQFFN-STHLYACGTNAYKPQAIII-------PASSLNANIDKKDAKNCCPFSSTQRNTAIIVD 170

  Fly   198 NGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDI- 261
            .       .||:.||..|.....||.|.....|. ..||      |:...|.|.:|.|:.|..:
Zfish   171 G-------ELYTATNVGFFGDKNVISRCHSRGTH-NNLE------LETVPKLLQEPVFISSTHVA 221

  Fly   262 --GEYVYFFFR--ETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSISGEFP 322
              |:.:.||..  :.:.:|......| ||:|:||..|.||...|...|.::.||:|.|. .|| .
Zfish   222 SEGKILLFFTENGDLSGDYFGNSFTV-SRVAQVCTDDNGGTLTLQRRWTSFAKAQLVCQ-QGE-D 283

  Fly   323 FYFNEIQSVYQL-PS-----DKSRFFATFTTS---TNGLIGSAVCSFHINEIQAAFNGKFKEQSS 378
            ..||::|.:.:| ||     ||:.|:..||:.   ::||  ||||:|.:.:|:|||:|.:|....
Zfish   284 LQFNKLQDIVKLSPSDSDSPDKTLFYGVFTSQRSVSSGL--SAVCAFSLTDIKAAFSGNYKTFDL 346

  Fly   379 SNSAWLPVLNSRVPEPRPGTC--VNDTSNLPDTVLNFIRSHPLMDKAVNHEHNNPVYYKRDLVFT 441
            ..:.|.|..|:   :.:.|.|  .|||    |:|||.::...|.:..|:......:....:..:|
Zfish   347 RGNHWSPQPNT---DGKLGKCGLFNDT----DSVLNRVKKSFLTENVVHPMDKTLLLSSAEEQYT 404

  Fly   442 KLVVDKIR-IDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFEVAPNEAIQVMEISQT 505
            :|.|.:.: :|  .|.|.:.|:.|..|.::|:| ....|..:.:.:.||: .|..|..:: :|::
Zfish   405 RLAVHRAQAVD--GQTYTILYLLTESGFLHKVV-LLAEGPHIIEEIQIFK-QPQIAKNIL-LSKS 464

  Fly   506 RKSLYIGTDHRIKQIDLAMCNRRYDNCFRCV--RDPYCGWDKEANTCRPY---ELDLLQDVANET 565
            :..|:||:...:.::.|:.|: .|.||..||  |||:|.||.|...|...   :..|.|||  |.
Zfish   465 KSVLFIGSSEGVFRVPLSNCS-AYPNCAECVLARDPFCAWDTETRACTAVSETKSTLRQDV--ER 526

  Fly   566 SDICDSSVLKKKIVVTYGQSVHLGCFVKIPEVLKNE--QVTWYHHSKDKGRYEIRYSPTK----- 623
            .::.:..:..:.......::.||...|.:|...|::  ::||            |:|...
Zfish   527 GNVTEQCIEFRNTAAVLFKTAHLNEIVVLPCQSKSKLAKMTW------------RFSNNSLVPQF 579

  Fly   624 -YIETTERGLVVVSVNEADGGRYDC---HLG-GSLLCSYNI---TVDAHRCTPPNKSN---DYQK 677
             |::.|: |.:|..|:......|.|   .|| ...:.::::   .|...|.:|.::..   |:..
Zfish   580 PYLQWTD-GSLVFPVSLETTNTYRCVSEELGFQQTIATFSVNLPVVPRSRMSPSHQQPEHIDFTT 643

  Fly   678 IYSDWCHEFEKYKTAMKSWEK---------KQAQCSTRQNFSS 711
            |..|......:.|...:|.||         |...|.:::::.|
Zfish   644 IELDESEPINEEKINDRSEEKQASTYHDAGKDTLCISQKSYYS 686

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2a	NP_477507.1	Sema_2A	66..523	CDD:200499	136/471 (29%)
		PSI	527..>550	CDD:396154	12/24 (50%)
		Ig_Semaphorin_C	573..668	CDD:409368	20/109 (18%)
		Ig strand A	573..579	CDD:409368	0/5 (0%)
		Ig strand B	583..591	CDD:409368	2/7 (29%)
		Ig strand C	601..607	CDD:409368	2/7 (29%)
		Ig strand C'	609..612	CDD:409368	0/2 (0%)
		Ig strand D	622..626	CDD:409368	1/9 (11%)
		Ig strand E	630..637	CDD:409368	2/6 (33%)
		Ig strand F	643..652	CDD:409368	4/12 (33%)
		Ig strand G	655..668	CDD:409368	2/15 (13%)
sema4aa	XP_009290884.1	Sema_4	43..482	CDD:200501	136/471 (29%)
		PSI	483..527	CDD:214655	19/46 (41%)
		Ig_Sema4B_like	540..624	CDD:409456	18/96 (19%)
		Ig strand A	540..546	CDD:409456	0/5 (0%)
		Ig strand B	550..558	CDD:409456	2/7 (29%)
		Ig strand C	564..570	CDD:409456	2/17 (12%)
		Ig strand C'	572..575	CDD:409456	0/2 (0%)
		Ig strand D	581..584	CDD:409456	1/2 (50%)
		Ig strand E	586..593	CDD:409456	2/7 (29%)
		Ig strand F	599..608	CDD:409456	2/8 (25%)
		Ig strand G	611..624	CDD:409456	0/12 (0%)