Protein Alignment Sema2a and Sema4b

DIOPT Version :9

• Sequence 1: NP_477507.1
• Gene: Sema2a / 36846
• FlyBaseID: FBgn0011260
• Length: 724
• Species: Drosophila melanogaster
• Sequence 2: NP_001163933.1
• Gene: Sema4b / 293042
• RGDID: 1307872
• Length: 821
• Species: Rattus norvegicus
• Alignment Length: 569
• Identity: 176/569 - (30%)
• Similarity: 276/569 - (48%)
• Gaps: 90/569 - (15%)

  Fly    58 VREFNCGKL-YYRTFHMNEDRDTLYVGAMDRVFRV--NLQNISSSNCNRDVINLEPTRDDVVSCV 119
            :|:|....: .|....:::|..||||||.:.:|.:  ||..:........:.:.:|.|..  .|.
  Rat    44 IRKFEAENISNYTALLLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADPDRKQ--QCS 106

  Fly   120 SKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPK-DYVIYANLTHLPRSEYVIGVGL---GI 180
            .|||....||:|:::::..:: ...|..|||.|.:|. .|:..|:.| |.|.|  .|..|   |.
  Rat   107 FKGKDPKRDCQNYIKILLPLN-SSHLLTCGTAAFSPLCAYINTASFT-LARDE--AGSVLLEDGK 167

  Fly   181 AKCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKY 245
            .:||:||...|||:.|:.       .||:||.:.|...|..|.|:            :..|..|.
  Rat   168 GRCPFDPNFKSTALVVDG-------ELYTGTVSSFQGNDPAISRS------------QSPRPTKT 213

  Fly   246 DS--KWLDKPNFVGSFDIGE----------YVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGK 298
            :|  .||..|.||.|..:.|          .:||||.||..|:......:.||:|||||.|.||:
  Rat   214 ESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGE 278

  Fly   299 NLLAHNWATYLKARLNCSISGE-FPFYFNEIQSVYQL---PSD--KSRFFATFTTSTN-GLI-GS 355
            .:|...|.::|||:|.||...: ||  ||.:|.|:.|   |.|  |:.|:..||:..: |.. ||
  Rat   279 RVLQQRWTSFLKAQLLCSRPDDGFP--FNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGS 341

  Fly   356 AVCSFHINEIQAAFNGKFKEQSSSNSAWLPVLNSRVPEPRPGTC---------VNDTSNLPDTVL 411
            |:|.|.:|::|.||:|.:|:.:.....|....:. ||.||||.|         :|.:..|||.||
  Rat   342 AICVFTMNDVQKAFDGLYKKVNRETQQWYTETHP-VPTPRPGACITNSARERKINSSLQLPDRVL 405

  Fly   412 NFIRSHPLMDKAVNHEHNNPVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYY 476
            ||::.|.|||..|   .:..:..:....:.::.|.  |:..|:..|.|.::||..||::|.|..
  Rat   406 NFLKDHFLMDGQV---RSRLLLLQPQARYQRVAVH--RVPGLHSTYDVLFLGTGDGRLHKAVTL- 464

  Fly   477 RNGESLSKLLDIFEVAP-NEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCV--RD 538
               :|...:::..:|.| .:.:|.:.:......||..:...:.|:.:|.|: .|..|..|:  ||
  Rat   465 ---DSRVHIIEELQVFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCS-LYPTCGDCLLARD 525

  Fly   539 PYCGWDKEANTCR---PYELDL-----LQDV--ANETSDICDSSVLKKK 577
            |||.|:  .:||:   .|:.||     :||:  || |.::|:.|..|.:
  Rat   526 PYCAWN--GSTCKLLSLYQPDLASRPWIQDIEGAN-TKELCNISSAKAR 571

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2a	NP_477507.1	Sema_2A	66..523	CDD:200499	150/493 (30%)
		Ig_Semaphorin_C	573..664	CDD:143180	1/5 (20%)
Sema4b	NP_001163933.1	Sema	46..509	CDD:417757	151/499 (30%)
		PSI	510..556	CDD:396154	17/48 (35%)
		Ig_Sema4B_like	577..661	CDD:409456
		Ig strand B	590..594	CDD:409456
		Ig strand C	602..606	CDD:409456
		Ig strand E	624..627	CDD:409456
		Ig strand F	637..642	CDD:409456
		Ig strand G	654..657	CDD:409456

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm45857
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11036
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	3.010