DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Sema2a and Sema5a

DIOPT Version :9

Sequence 1:	NP_477507.1	Gene:	Sema2a / 36846	FlyBaseID:	FBgn0011260	Length:	724	Species:	Drosophila melanogaster
Sequence 2:	NP_033180.2	Gene:	Sema5a / 20356	MGIID:	107556	Length:	1074	Species:	Mus musculus

Alignment Length:	713	Identity:	214/713 - (30%)
Similarity:	322/713 - (45%)	Gaps:	157/713 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    58 VREFNC-GKLYYRTFHMNEDRDTLYVGAMDRVFRVNLQNIS-----SSNCNRDVINLEPTRDDVV 116
            :|||.. ..:.:.....:..:..|.|||.:.:||:.|:::|     ...|:      |.|:.
Mouse    47 LREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECD------EATKK--- 102

  Fly   117 SCVSKGKSQIFDCKNHVRVIQSMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGLGIA 181
            :|.|||||: .:|:|::||:  :..||||:.|||||..|  .....:|::|......|.   |:|
Mouse   103 ACYSKGKSK-EECQNYIRVL--LVGGDRLFTCGTNAFTP--VCTIRSLSNLTEIHDQIS---GMA 159

  Fly   182 KCPYDPLDNSTAIYVENGNPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYD 246
            :|||.|..||||:...:|.      ||:.|..:|...|..|:|:  ..|...      .||.:|:
Mouse   160 RCPYSPQHNSTALLTASGE------LYAATAMDFPGRDPAIYRS--LGTLPP------LRTAQYN 210

  Fly   247 SKWLDKPNFVGSFDIGEYVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKA 311
            ||||::||||.|:|||.:.||||||.|||: :|||.|:||.|||||.|:||:.||...|.|::||
Mouse   211 SKWLNEPNFVSSYDIGNFTYFFFRENAVEH-DCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKA 274

  Fly   312 RLNCSISGEFPFYFNEIQSVYQLPSDKSRFFATFTTSTNGLIGSAVCSFHINEIQAAFNGKFKEQ 376
            |||||..||.|||:||:|..:.|| :....:..|||:.|.:..||||.|:::.|..||||.||.|
Mouse   275 RLNCSRPGEVPFYYNELQGTFFLP-ELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQ 338

  Fly   377 SSSNSAWLPVLNSRVPEPRP---------GTCVNDTS-NLPDTVLNFIRSHPLMDKAVNHEHNNP 431
            .:|.|||||     .|.|.|         |..||.|. ||.| ...||    ||.:.|......|
Mouse   339 ENSRSAWLP-----YPNPNPNFQCGTMDQGLYVNLTERNLQD-AQKFI----LMHEVVQPVTTVP 393

  Fly   432 VYYKRDLVFTKLVVDKIRIDILNQEYIVY--YVGTNLGRIYKI--VQYYRNGESLSKLLDIFEVA 492
            .:.:.:..|:.|.||.::    .:|.:|:  |:.|:.|.|.|:  .....:|..|.:.:::|...
Mouse   394 SFMEDNSRFSHLAVDVVQ----GRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPER 454

  Fly   493 PNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCV--RDPYCGWDKEANTCRPYEL 555
            .:|.|:.::|..::..|::|....:.:|.|..|: .:.....|:  :|||||||.....|...|.
Mouse   455 RSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCH-FHQTRSACIGAQDPYCGWDAVMKKCTSLEE 518

  Fly   556 DLLQDVANETSDICDSSVLKKKIVV--TYGQSVHLGCFVKIPEVLKNEQVTWYHHSKDKGRYEIR 618
            .|.....:::...|.:    :.:.|  ::|                    .|           ..
Mouse   519 SLSMTQWDQSIPTCPT----RNLTVDGSFG--------------------PW-----------SP 548

  Fly   619 YSPTKYIETTERGLVVVSVNEADG-----GRYDC-----------HLGG----------SLLCSY 657
            ::|..:.:.|..|..:......|.     |.:.|           ..||          |..|..
Mouse   549 WTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGI 613

  Fly   658 NITVDAHRCTPP------------NKSNDYQKIYSDWCHEF-----EKYKTAMKSWEKKQAQC 703
            ...|....|:.|            |:...|       |:|.     ..:.|....||:..|||
Mouse   614 GFQVRQRSCSNPTPRHGGRVCVGQNREERY-------CNEHLLCPPHVFWTGWGPWERCTAQC 669

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sema2a	NP_477507.1	Sema_2A	66..523	CDD:200499	171/475 (36%)
Sema2a	NP_477507.1	Ig_Semaphorin_C	573..664	CDD:143180	14/118 (12%)
Sema5a	NP_033180.2	Sema_5A	50..485	CDD:200524	172/481 (36%)
		PSI	486..533	CDD:396154	12/47 (26%)
		TSP1	544..595	CDD:214559	8/81 (10%)
		TSP1	598..651	CDD:214559	9/59 (15%)
		TSP1	656..702	CDD:214559	6/14 (43%)
		TSP1	787..839	CDD:214559
		TSP1	853..896	CDD:214559
		TSP1	899..940	CDD:214559
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3611
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R9633
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.840