Protein Alignment Sema2a and sema4b

DIOPT Version :10

• Sequence 1: NP_477507.1
• Gene: Sema2a / 36846
• FlyBaseID: FBgn0011260
• Length: 724
• Species: Drosophila melanogaster
• Sequence 2: XP_031755080.1
• Gene: sema4b / 100489037
• XenbaseID: XB-GENE-922109
• Length: 850
• Species: Xenopus tropicalis
• Alignment Length: 715
• Identity: 197/715 - (27%)
• Similarity: 323/715 - (45%)
• Gaps: 161/715 - (22%)

  Fly    11 ALLLLLCSSVSETAADY---ENTWNFYYERPCCTGNDQGNNNYGKHGADHVREFNCGKLYYRTFH 72
            |:..:||.|....|.:|   :.|:....||           ::....||       |..|:.|..
 Frog    22 AIFCVLCDSKFVNAIEYCGWDETFRGSPER-----------SFKTFEAD-------GVTYFTTLL 68

  Fly    73 MNEDRDTLYVGAMDRVFRVNLQNISSSNCNRDVINLEPTRDDVVSCVSKGKSQIFDCKNHVRVIQ 137
            :.:|...|||||.:.:|.:|..:.:|::.: ..|..:...:....|..|||....||:|:::::.
 Frog    69 LGKDGSALYVGAQEALFSLNSTDFTSADYH-SKIAWQADEEKKKECAFKGKDPQRDCQNYIKILL 132

  Fly   138 SMDQGDRLYVCGTNAHNPKDYVIYANLTHLPRSEYVIGVGL---GIAKCPYDPLDNSTAIYVENG 199
            .:: ...||.|||.|.:|.  ..|..::.....:..:|..:   |..:||:||..|||||.|:.
 Frog   133 QLN-STHLYTCGTFAFSPT--CTYIKISDFSLEKNALGHPILEDGKVRCPFDPEYNSTAIMVDG- 193

  Fly   200 NPGGLPGLYSGTNAEFTKADTVIFRTDLYNTSAKRLEYKFKRTLKYDSKWLDKPNFVGSFDIGE- 263
                  .:|:||.:.|...:.:|     |.:...|:..|.:.:|    .||..|.||||..|.|
 Frog   194 ------EMYAGTVSNFQGNEPII-----YKSHDSRITLKTENSL----YWLQDPAFVGSAYIQES 243

  Fly   264 -----------YVYFFFRETAVEYINCGKAVYSRIARVCKKDVGGKNLLAHNWATYLKARLNCSI 317
                       .:|:||.||..|:......:.||||||||.|:||:.:|...|.::|||:|.||.
 Frog   244 LPKGNPVGDDDKIYYFFSETGKEFDFFENTIVSRIARVCKGDLGGERVLQRRWTSFLKAQLICSC 308

  Fly   318 SGE-FPFYFNEIQSVYQL-PSDK----SRFFATFTTSTN--GLIGSAVCSFHINEIQAAFNGKFK 374
            .|: ||  ||.::.::.| |.::    :.|:..||:..|  |...||||:|.::|:|.||||.||
 Frog   309 PGDGFP--FNVLKDMFVLTPGEEHWKSTIFYGVFTSQWNKGGAGSSAVCAFSMDEVQNAFNGLFK 371

  Fly   375 EQSSSNSAWLPVLNSRVPEPRPGTC---------VNDTSNLPDTVLNFIRSHPLMDKAVNHEHNN 430
            |.:.....|....|| |||||||.|         :|.:..:||.||||::.|.|||.|:   .:.
 Frog   372 EVNRETQQWYTYTNS-VPEPRPGACITNSARDKKINSSLQMPDRVLNFVKDHFLMDSAI---RSQ 432

  Fly   431 PVYYKRDLVFTKLVVDKIRIDILNQEYIVYYVGTNLGRIYKIVQYYRNGESLSKLLDIFE----V 491
            |:..:..:.:.::.|.::|  .|.:.|.|.::||:.||::|.|       |::|::.|.|    .
 Frog   433 PLLLQSQIRYQQISVQRVR--GLKKTYDVLFLGTDDGRLHKAV-------SVNKMVHIIEEIKLF 488

  Fly   492 APNEAIQVMEISQTRKSLYIGTDHRIKQIDLAMCNRRYDNCFRCV--RDPYCGWDK--------- 545
            ...:.:|.:.:..::..:|..:...:.|:.::.|: .|.:|..|:  |||||.|:|
 Frog   489 QDMQPVQELLLDPSKGLIYASSRSAVVQVPVSNCS-MYRSCGECILSRDPYCAWNKKGCHSVPHH 552

  Fly   546 --------------EANT---CRPYELDLLQDVANETSDI----------CDSSVLKKKIVVTYG 583
                          :|||   |:|         :|.|..|          |....::.....|
 Frog   553 THDTKNQHWVQDIEDANTDAHCKP---------SNSTEAIGRAGKPDESECQEISMESNPANT-- 606

  Fly   584 QSVHLGCFVKIPEVLKN-EQVTWYHHSKDKGRYEIRYSPTKYIETTERGLVVVSVNEADGGRYDC 647
                |.|     ::|.| ....|.|:.|:        ..:.|.......|::||..:. .|.|:|
 Frog   607 ----LPC-----KLLSNLASRVWLHNGKE--------IDSSYFVLPSGDLILVSSPDR-VGTYEC 653

  Fly   648  647
             Frog   654  653

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2a	NP_477507.1	Sema_2A	66..523	CDD:200499	150/492 (30%)
		PSI	527..>550	CDD:396154	13/50 (26%)
		Ig_Semaphorin_C	573..668	CDD:409368	15/76 (20%)
		Ig strand A	573..579	CDD:409368	0/5 (0%)
		Ig strand B	583..591	CDD:409368	1/7 (14%)
		Ig strand C	601..607	CDD:409368	1/5 (20%)
		Ig strand C'	609..612	CDD:409368	1/2 (50%)
		Ig strand D	622..626	CDD:409368	1/3 (33%)
		Ig strand E	630..637	CDD:409368	2/6 (33%)
		Ig strand F	643..652	CDD:409368	3/5 (60%)
		Ig strand G	655..668	CDD:409368
sema4b	XP_031755080.1	Sema	55..520	CDD:472829	153/506 (30%)
		PSI	521..564	CDD:214655	11/43 (26%)
		Ig	594..669	CDD:472250	16/80 (20%)
		Ig strand B	605..609	CDD:409456	2/9 (22%)
		Ig strand C	617..621	CDD:409456	0/3 (0%)
		Ig strand E	637..640	CDD:409456	0/2 (0%)
		Ig strand F	650..655	CDD:409456	2/4 (50%)