DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Khc and Kif5a

DIOPT Version :9

Sequence 1:	NP_476590.1	Gene:	Khc / 36810	FlyBaseID:	FBgn0001308	Length:	975	Species:	Drosophila melanogaster
Sequence 2:	NP_001034089.1	Gene:	Kif5a / 16572	MGIID:	109564	Length:	1027	Species:	Mus musculus

Alignment Length:	946	Identity:	567/946 - (59%)
Similarity:	720/946 - (76%)	Gaps:	33/946 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 EDSIKVVCRFRPLNDSEEKAGSKFVVKFPNNVEENCISIAGKVYLFDKVFKPNASQEKVYNEAAK 74
            |.||||:|||||||.:|...|.||:..|..   ::.:.|.||.|:||:||.||.:||:||:..|.
Mouse     7 ECSIKVLCRFRPLNQAEILRGDKFIPIFQG---DDSVIIGGKPYVFDRVFPPNTTQEQVYHACAM 68

  Fly    75 SIVTDVLAGYNGTIFAYGQTSSGKTHTMEGVIGDSVKQGIIPRIVNDIFNHIYAMEVNLEFHIKV 139
            .||.||||||||||||||||||||||||||.:.|....||||||..|||||||:|:.||||||||
Mouse    69 QIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKV 133

  Fly   140 SYYEIYMDKIRDLLDVSKVNLSVHEDKNRVPYVKGATERFVSSPEDVFEVIEEGKSNRHIAVTNM 204
            ||:|||:|||||||||:|.||||||||||||:|||.|||||||||::.:||:|||||||:|||||
Mouse   134 SYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNM 198

  Fly   205 NEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALG 269
            |||||||||:||||:||||:|.::||||||||||||||||||||||||.||||||||||||||||
Mouse   199 NEHSSRSHSIFLINIKQENVETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALG 263

  Fly   270 NVISALADGNKTHIPYRDSKLTRILQESLGGNARTTIVICCSPASFNESETKSTLDFGRRAKTVK 334
            |||||||:|.|:::||||||:|||||:|||||.|||:.|||||:|:|::||||||.||:||||:|
Mouse   264 NVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIK 328

  Fly   335 NVVCVNEELTAEEWKRRYEKEKEKNARLKGKVEKLEIELARWRAGETVKAEEQINMEDLMEASTP 399
            |...||.|||||:||::|||||||....|..:.|||.||:|||.||.|...|::..||
Mouse   329 NTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGED------- 386

  Fly   400 NLEVEAAQTAAAEAALAAQRTALANMS--ASVAVNEQARLATECERLYQQLDDKDEEINQQSQYA 462
                     :|..|.|..:.....|.|  ..:|..|:.:...|..|||:||||||:|||||||..
Mouse   387 ---------SALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLI 442

  Fly   463 EQLKEQVMEQEELIANARREYETLQSEMARIQQENESAKEEVKEVLQALEELAVNYDQKSQEIDN 527
            |:||:|:::||||:.:.|.:.|.:|.|::.:|.||::||:||||||||||||||||||||||::.
Mouse   443 EKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEE 507

  Fly   528 KNKDIDALNEELQQKQSVFNAASTELQQLKDMSSHQKKRITEMLTNLLRDLGEVGQAIAPGESSI 592
            |::....|.:||.||.:...:..:|||:|:::|.||:|||.|:|..|:|||.|....:..||..:
Mouse   508 KSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMRDLSEFSVIVGNGEIKL 572

  Fly   593 DLKMSALAGTDASKVEEDFTMARLFISKMKTEAKNIAQRCSNMETQQADSNKKISEYEKDLGEYR 657
            .:::|       ..:||:||:|||:|||:|:|.|::.:||..:|..|.:.::|:....::|...:
Mouse   573 PVEIS-------GAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQ 630

  Fly   658 LLISQHEARMKSLQESMREAENKKRTLEEQIDSLREECAKLKAAEHVSAVNAEEK----QRAEEL 718
            ||||||||:::||.|.|:..|.|||.|||..|||.:|.|:|:|.|.|..|..::|    |.|||:
Mouse   631 LLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDELARLQAHETVHEVALKDKEPDTQDAEEV 695

  Fly   719 RSMFDSQMDELREAHTRQVSELRDEIAAKQHEMDEMKDVHQKLLLAHQQMTADYEKVRQEDAEKS 783
            :...:.||:..||||.||::.|||||..||..:||:||::|||.|..:::.||||:::.|:.|||
Mouse   696 KKALELQMENHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYERLKNEENEKS 760

  Fly   784 SELQNIILTNERREQARKDLKGLEDTVAKELQTLHNLRKLFVQDLQQRIRKNVVNEESEEDGGSL 848
            ::||.:....||.||:::||||||:|||:|||||||||||||||:..|::|: ...|.|:.||..
Mouse   761 AKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKS-AEMEPEDSGGIH 824

  Fly   849 AQKQKISFLENNLDQLTKVHKQLVRDNADLRCELPKLEKRLRCTMERVKALETALKEAKEGAMRD 913
            :||||||||||||:||||||||||||||||||||||||||||.|.|||||||.||||||||||:|
Mouse   825 SQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKD 889

  Fly   914 RKRYQYEVDRIKEAVRQKHLGRRGPQAQIAKPIRSG 949
            ::|||.||||||||||.|..|:||..||||||:|.|
Mouse   890 KRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPG 925

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Khc	NP_476590.1	KISc_KHC_KIF5	10..333	CDD:276820	240/322 (75%)
		KISc	12..340	CDD:214526	242/327 (74%)
		Apolipoprotein	595..744	CDD:279749	65/152 (43%)
Kif5a	NP_001034089.1	KISc_KHC_KIF5	7..327	CDD:276820	240/322 (75%)
		Microtubule-binding	174..315		113/140 (81%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000269\|PubMed:21976701	271..361		59/89 (66%)
		Interaction with BICD2. /evidence=ECO:0000250\|UniProtKB:Q12840	353..1027		308/597 (52%)
		Smc	<412..759	CDD:224117	164/353 (46%)
		DUF5401	<630..>902	CDD:375164	168/272 (62%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	906..937		12/20 (60%)
		Globular	907..1027		12/19 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	643	1.000	Domainoid score	I164
eggNOG	1	0.900	-	-		E2759_KOG0240
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1143	1.000	Inparanoid score	I212
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG52972
OrthoDB	1	1.010	-	-		D1334528at2759
OrthoFinder	1	1.000	-	-		FOG0000669
OrthoInspector	1	1.000	-	-		otm42515
orthoMCL	1	0.900	-	-		OOG6_100344
Panther	1	1.100	-	-	O	PTHR24115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2595
SonicParanoid	1	1.000	-	-		X952
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.870