Protein Alignment SP2353 and lama4

DIOPT Version :10

• Sequence 1: NP_611082.2
• Gene: SP2353 / 36771
• FlyBaseID: FBgn0034070
• Length: 1361
• Species: Drosophila melanogaster
• Sequence 2: NP_001034154.1
• Gene: lama4 / 566796
• ZFINID: ZDB-GENE-040724-213
• Length: 1871
• Species: Danio rerio
• Alignment Length: 1409
• Identity: 254/1409 - (18%)
• Similarity: 446/1409 - (31%)
• Gaps: 518/1409 - (36%)

  Fly   161 EDSNSET-QEQKQKSLNLQQQQQQQQKVNS-----NDYYISAESINFNSESEGQEQQDV------ 213
            ||:..:. .:.|...|.||:::.|||::..     :...::|.::...:.....|.:||
Zfish   553 EDALKQAINKYKSNQLKLQRREAQQQRLKDIQDGVDQTIVNAAAVLSEANHTVLEVEDVITNVRE 617

  Fly   214 --ADFKAASTMLPSSVPPTPSRSTTRATASTTTTTTTTRRSPTRAPKQRVDVDYG-EGDGSDDYS 275
              |:...|..:|        .|.|.:.:.|.............:..:...:..:. :|..::.:.
Zfish   618 QHAEIDGARVLL--------ERRTEKISQSDRELVLRAEDHADQLQRHAEEPQHNLKGSDANGHV 674

  Fly   276 YSYKSDMSVY-------DDVN--------------------NNQLNLRRNSKSQT--------YQ 305
            ....:..:||       ||.|                    ::||||.|:..::.        :|
Zfish   675 QKALNAFNVYDNIVKSVDDANITSLTALNISSRTHGAVDKLSSQLNLMRSQSAKVLNEASSLHFQ 739

  Fly   306 QTSNTNTAKNRRKYPNVT-------SNKV--------QMHITRETNRLE-NISAAEPAVVGAAMT 354
            |..:.:...:.:||...|       |.|:        .:|..|...||: ....||..:..:|..
Zfish   740 QQDSESIVLDNKKYIEETNEMMDGSSKKIAKIITEINAIHTDRTPKRLQFTQEVAEGTLNRSAEV 804

  Fly   355 TDRSTPTCNLDCGSDGICALEATAASSRCLCPFGKT---GNGCQEDIRAHVPRFAKR-------- 408
            .::.||........||....||.:|::     |.:|   .....:|:.|.||....:
Zfish   805 LEKVTPILKRVEQWDGNMKSEAYSAAA-----FDRTVLSAGEAVKDLSAIVPELLSKLRVVEEKK 864

  Fly   409 -------SWLAFPAL----HGAYKHVQLRIEFRPES---------FDGI---------ILLSGER 444
                   |.:....|    ....|.||:.::|..:|         .|.:         |.:..::
Zfish   865 PVNNVTTSIMKIRGLIAQARSVAKKVQVSMKFDGQSAVEVHPHTNLDELKTVTSISFYIRVDPDK 929

  Fly   445 DDLTGDFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYR------------HRWDA 497
            |.:...|:..|            |...|. |....:.:...|.|.:|:            ....:
Zfish   930 DPIEDRFLLYL------------GDKQGK-RDYMGLAIKNDNLVYVYKLGEEEVEIPLSSKPVSS 981

  Fly   498 WLVLNHGTKVQ--GRSNGLFSRITFR------------EPV---------------FLGGIGNIT 533
            |..:.:..||:  ||...:|..:..:            |||               .:||:....
Zfish   982 WPPVFNFVKVERLGRHGKVFLTVPSQDTTAEQKFIQKGEPVGTDTLFDLDPKDMVFIVGGVPPGV 1046

  Fly   534 GLAKRLPLAEGFAGCIRRFVANEHDYKFTEHPLGDVINGFDIQDCSTDKCVRYPCQHGGKCLPSD 598
            .:...|.||. |.|||.....|           .|||:.::.:...
Zfish  1047 TVPPILSLAP-FVGCIELETLN-----------NDVISLYNFKQLH------------------- 1080

  Fly   599 QGAICLCPIGFVGDLCEIRMDLQVPAFNGSSFLRYAPLGDSALIWLELKVTLKPEQADGLILYSG 663
                              :||::..          ||.....|.:.:.:||
Zfish  1081 ------------------KMDVKAS----------APCPRHKLAFSQSRVT-------------- 1103

  Fly   664 PEHRGDFIALYLNDGFVEFAFDLGSGPALVRSEHSLSLGQWHTIKISRTARLAVL-KVDKHQEVL 727
                             .:.|| |:|.|||.:             |.|..::.|: :.|  .||.
Zfish  1104 -----------------SYLFD-GTGYALVNN-------------IERRGKIGVVTRFD--IEVR 1135

  Fly   728 TISSNGFWHLSLDQ-NLFVGGVNHVDRLPLDLKYKPFFVGCIQRIDINGHSLGIVAEALGGSNIG 791
            |:::||...|.::: |.||          |:||              ||. |.::.: .|.:|
Zfish  1136 TVANNGILFLMVNETNFFV----------LELK--------------NGF-LRLMYD-FGFAN-- 1172

  Fly   792 NCPHACVARPCGPLAECVPQMESYECRCSIHNERCNKAAEVPPEQLPELALHKSKVLETKDNGEA 856
                       ||:.     :|:...:..|::.|.::.:.:         .|.|
Zfish  1173 -----------GPVI-----IETNLAKLQINDARYHEVSVI---------YHHS----------- 1201

  Fly   857 AKKVSGLAHKKHSKKHRNLHKPTPATSTTTSTTSTTTTTTEAPSERTEEATAGALSNEEIEDDII 921
             ||:..|..:.|.|...:..||.|.:.....         .|||...:       |..|:...|.
Zfish  1202 -KKIILLVDRSHVKSFESEKKPLPFSDIYIG---------GAPSRILQ-------SRPELTSLIG 1249

  Fly   922 FR-LVQQQQQQKE----LKKQHQQTTTTAPATSTTSSG-PSKA----KPRLSGKHHASKHEHHLK 976
            .: .|:..|.|||    |::|         .|...||| |.::    |...:|..:.|..: .:.
Zfish  1250 LKGCVKGFQFQKEDFNLLEEQ---------GTIGISSGCPEESFMSHKAYFTGNGYLSSSD-KIS 1304

  Fly   977 PNAAFTRKLSRLPTHYESFQTNPDSDILTF--EDNNDWVTSL----------------QQQEYGD 1023
            |..:|...|        ||:|...|.:|.:  :.::::..||                |::.|.|
Zfish  1305 PFQSFEGGL--------SFKTLQPSGLLFYHRDGSDEFSLSLENGAVVLQAKGTRVKSQKKHYSD 1361

  Fly  1024 A----MAASQVPLAFE-------DASPGTPRSSDNNEDDENAFVFDESLF--------------- 1062
            .    :.||.....:|       ......|.|:..:::...||.:..|..
Zfish  1362 GRSHFLVASVTNQKYEIVIDDKDKQDKKKPSSTSQSDNTPKAFYYGGSTSSSILNLTGCISYAYI 1426

  Fly  1063 ---DASDGTEEYQRKQLAQDMKRIMSNSNAHSSHKKAEVQFPPQG---------------SQEVG 1109
               |.....|::||           .:.|..:|.:...::.||..               |::||
Zfish  1427 SRQDRDIEPEDFQR-----------YSENVQASLQDCPIERPPSALLKDSRDSSRRSAGHSRKVG 1480

  Fly  1110 TANEDTSQYSDDYNDDELLTPVMQGGEEVKLEQHTSSTPQT--HTDWSLLKKFDLSAEHQSQVQG 1172
               .|.|........:.|..|     |:.:...:.||.|:.  |.:     .:..||..:.:..|
Zfish  1481 ---RDESGLPHGLKSNPLNIP-----EKEEASCYLSSRPRATPHAN-----HYGGSAHSRHEYTG 1532

  Fly  1173 VRKNFGACFAGSDSYFHYNDADTMSQVISYSIDLNLRIKTHSENGVILWTGRQGTTEEHDDYLSL 1237
            :                       :..|.:....:|.:||.|..|:|.:.    .....::.::|
Zfish  1533 I-----------------------ASAIKHESHFSLSLKTRSAFGLIFYL----ADASGENSMAL 1570

  Fly  1238 GIEQGYLHFRYDLGSGEVDIRFNGTKVSDGLWHRVRAIRNSQEGYLEVDGRKTVTLRAPGKLRQL 1302
            .:....|.|.:..|..:|.|| :..|.:||.||.:..||....|.:.:||...:..||||:...|
Zfish  1571 FLTHARLVFTFSSGQSQVRIR-SKEKYNDGQWHDIIFIRAGNMGRMLIDGLTVLEDRAPGRNTSL 1634

  Fly  1303 NTDTGLYVGGMPDVGYFTHQRY-------FSGIVGCISEIVLAG 1339
            .....|||||:|     .||..       .|...||:..:.|.|
Zfish  1635 LVQDPLYVGGVP-----PHQAMKNIQRTSVSSFTGCVRSLQLNG 1673

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SP2353	NP_611082.2	FXa_inhibition	53..82	CDD:464251
		LamG	404..555	CDD:238058	36/228 (16%)
		EGF	583..612	CDD:394967	0/28 (0%)
		LamG	625..774	CDD:238058	28/150 (19%)
		LamG	1205..1340	CDD:214598	41/142 (29%)
lama4	NP_001034154.1	Laminin_EGF	99..152	CDD:395007
		EGF_Lam	155..207	CDD:238012
		EGF_Lam	209..247	CDD:214543
		EGF_Lam	256..300	CDD:214543
		Laminin_I	356..611	CDD:310534	11/57 (19%)
		Cast	<490..817	CDD:431111	46/271 (17%)
		Laminin_II	790..919	CDD:368703	24/133 (18%)
		LamG	893..1067	CDD:238058	31/187 (17%)
		LamG	1104..1259	CDD:238058	50/251 (20%)
		LamG	1289..1422	CDD:238058	23/141 (16%)
		LamG	1535..1672	CDD:238058	40/146 (27%)
		LamG	1696..1847	CDD:238058