DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment SP2353 and AGRN

DIOPT Version :9

Sequence 1:	NP_001286474.1	Gene:	SP2353 / 36771	FlyBaseID:	FBgn0034070	Length:	1361	Species:	Drosophila melanogaster
Sequence 2:	NP_001292204.1	Gene:	AGRN / 375790	HGNCID:	329	Length:	2068	Species:	Homo sapiens

Alignment Length:	1414	Identity:	289/1414 - (20%)
Similarity:	428/1414 - (30%)	Gaps:	551/1414 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 LSSLSSASIAVGAIPEPEEAAFQ---GHC--GHT-------SPCEQLCYEIHDGMYECDCIEGYE 74
            |..|..:|:|....|....:.:.   |.|  |.|       |.|.  ..::..|:.|.:.:||.|
Human  1085 LEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCP--ATKVFQGVLELEGVEGQE 1147

  Fly    75 LNKNGYSCQVINATSNSLDGRGKSDE---DVLYQ----KGASFSAKLANDDGGKSMA-------K 125
            |   .|:.::.:..|.......:|.|   |.|::    |....|.:|.:...|||:.       .
Human  1148 L---FYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFD 1209

  Fly   126 STFALSAAQSASPAGNENGEDESGSEGDSYDYGELEDSNSETQEQKQKSLNLQQQQQQQQKVNSN 190
            .|.|..|...|...                        ..:.|..:::||.:::..|:..:....
Human  1210 PTTAFRAPDVARAL------------------------LRQIQVSRRRSLGVRRPLQEHVRFMDF 1250

  Fly   191 DYYISAESINFNSESEGQEQQDVADFKAASTMLPSS--VPPTPSRSTTRATASTTTTTTTTRRSP 253
            |::.:.    ....:.|............::.||||  .|..|..|.|....:.||...||||.|
Human  1251 DWFPAF----ITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPP 1311

  Fly   254 TRAPKQRVDVDYGEGDGSDDYSYSYKSDMSVYDDVNNNQLNLRRNSKSQTYQQTSNTNTAKNRRK 318
            |.||.:                                                     ...||
Human  1312 TTAPSR-----------------------------------------------------VPGRR- 1322

  Fly   319 YPNVTSNKVQMHITRETNRLENISAAEPAVVGAAMTTDRSTPTCNLDCGSDGICALEATAASSRC 383
                                      .||........| |.|     |...|.|...|......|
Human  1323 --------------------------PPAPQQPPKPCD-SQP-----CFHGGTCQDWALGGGFTC 1355

  Fly   384 LCPFGKTGNGCQEDIRAHVPRFAKRSWLAFPALHGAYKHVQLRIEFRPESFDGIILLSGERDDLT 448
            .||.|:.|..|::.:.|.||.|..||:||||.|. ||..::|.:|||.....|::|.:|   :..
Human  1356 SCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLR-AYHTLRLALEFRALEPQGLLLYNG---NAR 1416

  Fly   449 G-DFMALLLNKGFVEFWFDCGSGVGSVRSRETILLNEWNSVIIYRHRWDAWLVLNHGTKVQGRSN 512
            | ||:||.|..|.|:..||.|||...:.|...:...:|:.:.:.||.....|.::..|.|.|.|.
Human  1417 GKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESP 1481

  Fly   513 GLFSRITFREPVFLGGI--GNITGLAKRLPLAEGFAGCIRRFVANEHDYKFTEHPLGDVINGFDI 575
            .....:.....:|:||:  .......:|..:..|..||||....|....:....| |....|..:
Human  1482 SGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGP-GAATRGSGV 1545

  Fly   576 QDCSTDKCVRYPCQHGG-----------------------------KCLPS------------DQ 599
            .:|....|:..|| |||                             .|.|:            :.
Human  1546 GECGDHPCLPNPC-HGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEG 1609

  Fly   600 GAICLCPIGFVGDLCEIR--MDLQVP---AFNGSSFLRY-------APLGDSALIWLELKVTLKP 652
            ||.|.||:|..|..|:..  .|...|   .|||.|.|..       ..||:.    :.|:|....
Human  1610 GAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEK----MALEVVFLA 1670

  Fly   653 EQADGLILYSG--PEHRGDFIALYLNDGFVEFAFDLGSGPALVRSEHSLSLGQWHTIKISRTARL 715
            ....||:||:|  .:.:|||::|.|.|..:||.:|||.|.|::||...::||.|..:.:.|..|.
Human  1671 RGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRK 1735

  Fly   716 AVLKVDKHQEVLTIS--SNGFWH--LSLDQNLFVGGVNHVDRLPLDLKYKPFFVGCIQRIDINGH 776
            ..|:|.....||..|  |....|  |:|.:.|:|||.....:|.........|.|.||.:.:.|.
Human  1736 GALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGR 1800

  Fly   777 SLGIVAEALGGSNIGN-CPHACV---ARPCGPLAECVPQMESYECRC--SIHNERCNKAAEVPPE 835
            .|......|...::.: ..|.|.   ..||...|.|||:..:|.|.|  ......|.|
Human  1801 QLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEK------- 1858

  Fly   836 QLPELALHKSKVLETKDNGEAAKKVSGLAHKKHSKKHRNLHKPTPATSTTTSTTSTTTTTTEAPS 900
                                      ||..|
Human  1859 --------------------------GLVEK---------------------------------- 1863

  Fly   901 ERTEEATAGALSNEEIEDDIIFRLVQQQQQQKELKKQHQQTTTTAPATSTTSSGPSKAKPRLSGK 965
                                                               |:|
Human  1864 ---------------------------------------------------SAG----------- 1866

  Fly   966 HHASKHEHHLKPNAAFTRKLSRLPTHYESFQTNPDSDILTFEDNNDWVTSLQQQEYGDAMAASQV 1030
                                              |.|.|.| |...:|      ||.:|:..|::
Human  1867 ----------------------------------DVDTLAF-DGRTFV------EYLNAVTESEL 1890

  Fly  1031 PLAFEDASPGTPRSSDNNEDDENAFVFDESLFDASDGTEEYQRKQLAQDMKRIMSNSNAHSSHKK 1095

Human  1891 ----------------------------------------------------------------- 1890

  Fly  1096 AEVQFPPQGSQEVGTANEDTSQYSDDYNDDELLTPVMQGGEEVKLEQHTSSTPQTHTDWSLLKKF 1160
                           |||               .||                |:|....:|..:.
Human  1891 ---------------ANE---------------IPV----------------PETLDSGALHSEK 1909

  Fly  1161 DLSAEHQSQVQGVRKNFGACFAGSDSYFHYNDADTMSQVISYSIDLNLRIKTHSENGVILWTGRQ 1225
            .|.:.|                                       ..|.::|.:..|::||:|:
Human  1910 ALQSNH---------------------------------------FELSLRTEATQGLVLWSGK- 1934

  Fly  1226 GTTEEHDDYLSLGIEQGYLHFRYDLGSGEVDIRFNGTKVSDGLWHRVRAIRNSQEGYLEVDGRKT 1290
              ..|..||::|.|..|:|...|:|||..|.:| :...|:...|.||.|.|..:||.|:|.....
Human  1935 --ATERADYVALAIVDGHLQLSYNLGSQPVVLR-STVPVNTNRWLRVVAHREQREGSLQVGNEAP 1996

  Fly  1291 VTLRAPGKLRQLNTDTGLYVGGMPD--VGYFTHQRYFSGIVGCISEIVL 1337
            ||..:|....||:||..|::||:|:  ||....:.|.:|.|||:.::|:
Human  1997 VTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVV 2045

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SP2353	NP_001286474.1	FXa_inhibition	53..82	CDD:291342	8/28 (29%)
		LamG	404..555	CDD:238058	48/153 (31%)
		EGF	583..612	CDD:278437	14/69 (20%)
		LamG	625..774	CDD:238058	52/161 (32%)
		LamG	1205..1340	CDD:214598	48/135 (36%)
AGRN	NP_001292204.1	NtA	44..146	CDD:308653
		KAZAL	196..242	CDD:197624
		KAZAL	272..317	CDD:197624
		KAZAL_FS	349..389	CDD:320757
		KAZAL_FS	421..461	CDD:238052
		KAZAL	489..534	CDD:197624
		KAZAL	554..599	CDD:197624
		KAZAL	619..664	CDD:197624
		KAZAL	704..750	CDD:197624
		Laminin_EGF	793..835	CDD:278482
		Laminin_EGF	847..>883	CDD:278482
		KAZAL	922..969	CDD:197624
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	995..1096		4/10 (40%)
		SEA	1130..1254	CDD:214554	27/150 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1277..1334		22/136 (16%)
		EGF	1333..1363	CDD:306513	11/35 (31%)
		Laminin_G_1	1400..1531	CDD:278483	37/133 (28%)
		EGF_CA	1555..1585	CDD:238011	5/30 (17%)
		Laminin_G_1	1668..1803	CDD:278483	44/134 (33%)
		EGF_CA	1825..1857	CDD:238011	9/31 (29%)
		Laminin_G_1	1921..2051	CDD:278483	47/129 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3509
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D17288at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10574
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.920