DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Zasp52 and Synpo2

DIOPT Version :9

Sequence 1:	NP_001027420.2	Gene:	Zasp52 / 36740	FlyBaseID:	FBgn0265991	Length:	2194	Species:	Drosophila melanogaster
Sequence 2:	NP_536699.2	Gene:	Synpo2 / 118449	MGIID:	2153070	Length:	1257	Species:	Mus musculus

Alignment Length:	1749	Identity:	338/1749 - (19%)
Similarity:	518/1749 - (29%)	Gaps:	694/1749 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 IKLSRFDAQPWGFRLQGGTDFAQPLLVQKVNAGSLSEQAGLQPGDAVVKINDVDVFNLRHKDAQD 73
            |.:|.....|||||||||.:..|||.|.|:.:.|.:..:||:.||.||.||.....:|.:.:
Mouse     7 ICISMTGGAPWGFRLQGGKEEQQPLQVAKIRSQSKASGSGLREGDEVVSINGNPCADLTYPE--- 68

  Fly    74 IVVRSGNNFVITVQRGGSTWRPHVTPTGNVPQPNSPYLQTVTKTSLAHKQQDSQHIGCGYNNAAR 138
                     ||.:..|                        :|         ||.|:     ...|
Mouse    69 ---------VIKLMEG------------------------IT---------DSLHL-----LVKR 86

  Fly   139 PFSNGGDGGVKSIVNKQYNTPVGIYSDESIAETLSAQAEVLAGGVLGVNFKKNEKEYQGDRSEVL 203
            |.|                         ..:|||.:::|..
Mouse    87 PSS-------------------------GTSETLDSESETT------------------------ 102

  Fly   204 KFLREEETGQSTPAFGNSHYEHDAPQQ---LQQPQQQYNQHQQHYHQQQQ------QQQSSTTRH 259
                           .:.|..|:.|.:   ||..|....|.:..:....|      :.||:...:
Mouse   103 ---------------NHQHLTHEGPMESTTLQIQQATETQSEDFFLAPVQTKVPLTEDQSNAWGY 152

  Fly   260 VSAPVNSPKPPSTGGLPTGQNICTECERLITGVFVRIKDK------------NLHVECFKCATCG 312
            ...|.....||..|         ::...|:..|.:|.|.:            :|..|..:| |.|
Mouse   153 AECPKEEQAPPMLG---------SQEGHLVEEVILRQKAEAGQPGHVVELQLSLSKERHQC-TSG 207

  Fly   313 TSLKNQGY-------YNFNNKLYCDIHAKQAAINNPPTGTEGYVPVPIKPNTKLSASTISSALNS 370
            ..:..||.       .:::::|...:|     ||:        :|.|.|.:|.|::|| ||....
Mouse   208 PIVTLQGNDKSTSPDPDWSSQLERTVH-----INS--------IPAPEKADTSLTSST-SSGREL 258

  Fly   371 HGYGGHSNGYSNGNSTPAPAPV--ASSQATATVATVAPSAATAATAAATPQAATATDSPAATASS 433
            ....|...|   |...|....:  .|.:..|....:    .|.....|:|.....:::|
Mouse   259 RVIQGRDPG---GAGLPQVEVILDCSDRLKAEECRL----QTGRGCVASPVEGGRSEAP------ 310

  Fly   434 SDNMSAYVADEPSSIYGQISAESVALAPPPPQPPTAGGGDQPFEYVTLTGNVIRSVQAPGKGACP 498
                       ||.:...:|:|.           |..|.||            ||    ||....
Mouse   311 -----------PSLVSFAVSSEG-----------TEHGEDQ------------RS----GKDQSR 337

  Fly   499 SYKVNQGYARPFGAAAPKSPVSYPPQQQQQ--------------SPRPAPGGQNPYA-------- 541
            .:|....:||      .:...|...:|.::              :|.|...|...:.
Mouse   338 PHKHRARHAR------LRRSESLSEKQVKEAKSKCKSIALLLTDAPNPNSKGVLMFKKRRRRARK 396

  Fly   542 -TLPRSNVGQQGGEAVEELQPEFEEEDCYEMD-----IEVALAASRQSQ-------------RGS 587
             ||.....|        ||:.|.|||:..|..     .|||...:.:|:             :..
Mouse   397 YTLVSYGTG--------ELEREEEEEEDQEAGDKDEISEVAFLGTSESEVDEELLSDVDDNTQVV 453

  Fly   588 SFTWPPPQDDSHLAPTAAPLYIPPPETQHVVVSNPVQQVPPLPPGGATARLDPQPVVGTSANGAP 652
            :|.|     ||.|......|             |...::..||              .|:..||
Mouse   454 NFDW-----DSGLVDIEKRL-------------NRGDKMEMLP--------------DTTGKGA- 485

  Fly   653 QWQSYSAPQLTTASARQLAEQESSSDSYTST-----------STTTTTTSEEYQRMYAAQVQAYQ 706
                     |..|..|:..||.::.:....|           .|....|...|||    :.::.:
Mouse   486 ---------LMFAKRRERMEQFTAQNEEEKTGGMAGGGPDALQTDGLRTMTSYQR----KEESVR 537

  Fly   707 MQEQSGSEFDYQVDYASTQDSVQDYPSG-------------RRSAQECVDSL------------- 745
            || .|.||..:|:..:......|:..||             .|:|:..:.|:
Mouse   538 MQ-SSVSESSFQMGRSLASVPQQNGFSGVSETAGAQRMFPMNRTAKPFLGSMNQPAAPFSPTRSV 601

  Fly   746 AVPLSTYKLVDMVREVTPSP------VTTPTQTPAPAAPTTRRVVFNDEPEIKELPQ-LPAELET 803
            ..|:|.:........|:|.|      |::|...||...|               .|| .|.....
Mouse   602 TSPISDFPAPPPYSAVSPPPEAFSRGVSSPVAGPAQPPP---------------WPQPAPWSQPA 651

  Fly   804 IPEASEAVEDREG--LVIEQRCQILESERKFQPTPEIKIEIAPVRQIPPTKI-PNPMPKEWINPM 865
            ..::||.:..|:.  .|..:|..||:..::...|       .|:.....||: |||       .:
Mouse   652 FYDSSEQIASRDERIAVPAKRTGILQEAKRRGTT-------KPMFTFKETKVSPNP-------EL 702

  Fly   866 IRVLTTA-----------PEVPFHLVECPFPRPCGDDFEAEAAAA----EAAKTQEVPEPLPPQ- 914
            :.:|..|           ||               :|:.:..|.|    :::..|:.|.|:.|:
Mouse   703 LSLLQNAEGKRGTGGDSGPE---------------EDYLSLGAEACNFMQSSAKQKTPPPVAPKP 752

  Fly   915 -----VSAAPPATVSV--EPSPAPLRE---------SPPRGSRLSQAMVTAPEFELKFAPPADQG 963
                 .|:.|.|.||.  .|..||.:.         :||:.:.:|...:..|.     ||||
Mouse   753 AVKSPSSSQPVAPVSPVWSPGVAPAQRPAFSTSNPPNPPQVTAVSSIKIAQPA-----APPA--- 809

  Fly   964 IPLPEETEPYMPPPIDTKPYLREDYRPKSPFVSALTTAPDRPFEGHFDKDVPIHMIDLPTPKEHL 1028
                                     ||    .|||..|  .||:|  .:.|.:.....|.|....
Mouse   810 -------------------------RP----ASALNLA--GPFKG--PQAVVVSHNYTPKPSAPT 841

  Fly  1029 SMCDALCTAPERGYTPLNPENAMHRVDEEQKQQELKKREFQVLDHEEELGIRPEPPQSVEYYETR 1093
            .:.:|   ||.....|.|....|.....:...:...:.|..|:|.:           :|:.:..|
Mouse   842 PLVNA---APAGAGGPSNELPGMSGKGAQLFAKRQSRMEKYVVDSD-----------TVQAHTVR 892

  Fly  1094 RDQPRKSSAFAAMQAFQPSREPLSSNTVSNAGSVADTPRASIVSALKEETDLEYQKYLKAQQRNQ 1158
            ...|..|         .|:....||| |.....||..|
Mouse   893 AQSPTPS---------LPASWKYSSN-VRAPPPVAYNP--------------------------- 920

  Fly  1159 KRLDYFHQKEEELSGLQ----GQQLTQLQRELSNQQQNLLSQQQLQQSKLLQLQQCVQSQELQQQ 1219
                 .|.....|:.::    |.|.::..::...:..|.|              ..::.|..|..
Mouse   921 -----IHSPSYPLAAIKSQPPGAQASKTSKKKGKKPLNTL--------------DVMKHQPYQLN 966

  Fly  1220 VQHLTQKSQQQPPQANQQQQQQQQQRGTQQQQHSQVTQRTQQQQQQVPQQVTQQQQQEHSLLSQT 1284
            ....|    .|||.:..         |..|:...:|:.....:|...|:
Mouse   967 ASLFT----FQPPDSKD---------GLPQKSTVKVSSAPAMKQALPPR---------------- 1002

  Fly  1285 TLAETQTLQANAQSQSSASYSSKATACSNSSSTVPPANTS-----TAFAPAP---APAPTSIPVR 1341
                    |||..|.::|..||..:.         ||.||     .|.|.:|   :|.|.|:|..
Mouse  1003 --------QANVGSPTNAQASSVYSV---------PAYTSQPNFFAAEATSPVSASPVPVSVPTS 1050

  Fly  1342 PSAIAVQSSYCSSQFDVHELIEETAEELEHSEVLFPPPSPLSHLTKQGKAVQSGLHKADSIP--- 1403
            |...:..:||                        |..|.|.....|.|..||.. .::.|:|
Mouse  1051 PKQESTSTSY------------------------FVAPRPKFSAKKSGVTVQEN-GRSLSLPGRS 1090

  Fly  1404 ----------KYQR--NWTVLPT---QSPIRTPEPQELRENVPLAFVDAPKAPVTSDSSTVHRPI 1453
                      .||.  |::..||   :...:.|.|.|.....||..||....|.....|.|...:
Mouse  1091 APPIISASPWLYQSACNYSSKPTFELEKANKRPTPWEAAARSPLGLVDDAFRPRNIQESIVAHVV 1155

  Fly  1454 AQVAAPTTVVAPSREREKERRPQLS-VPIIVEDRSGPVTMAFQPLDELVRPDQALTPTRPY--TP 1515
            :  ||...|...|:|..|||   || ||     ::....|:|....|...|    :|...:  :.
Mouse  1156 S--AARRKVSPGSQEDWKER---LSFVP-----QTQKTNMSFSERQEYPAP----SPVNSHVSSH 1206

  Fly  1516 SLTNKPAPIVPFYQTEEKLVFEECSATHARNYNELNASPFPDRTRS----PAPGPPPNP 1570
            ||.:...|.| .|:.|.:              |:|.....  .|||    |..|...||
Mouse  1207 SLYSSQLPYV-CYRQESR--------------NDLKTMSM--ETRSEYCLPLGGYDYNP 1248

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Zasp52	NP_001027420.2	PDZ_signaling	6..87	CDD:238492	28/77 (36%)
		DUF4749	150..>217	CDD:292558	4/66 (6%)
		LIM_ALP_like	282..333	CDD:188746	12/69 (17%)
		LIM1_Enigma_like_1	2020..2073	CDD:188839
		LIM	2081..2132	CDD:259829
		LIM3_Enigma_like_1	2140..2193	CDD:188845
Synpo2	NP_536699.2	PDZ_signaling	7..85	CDD:238492	33/127 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	24..52		10/27 (37%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	88..112		7/87 (8%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	207..272		19/81 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	320..359		13/71 (18%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:16309678	388..396		0/7 (0%)
		Interaction with YWHAB. /evidence=ECO:0000269\|PubMed:15883195	551..557		0/5 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	581..817		61/316 (19%)
		Interaction with YWHAB. /evidence=ECO:0000269\|PubMed:15883195	599..804		49/248 (20%)
		PPPY motif. /evidence=ECO:0000250\|UniProtKB:Q9UMS6	611..614		0/2 (0%)
		Interaction with ACTN2. /evidence=ECO:0000250\|UniProtKB:Q9UMS6	656..917		71/354 (20%)
		F-actin bundling activity. /evidence=ECO:0000250\|UniProtKB:Q9UMS6	656..909		68/345 (20%)
		F-actin binding. /evidence=ECO:0000250\|UniProtKB:Q9UMS6	656..796		35/168 (21%)
		Actin binding. /evidence=ECO:0000269\|PubMed:11673475	740..893		42/207 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	832..863		8/33 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	930..952		2/21 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	970..1012		13/78 (17%)
		Interaction with ZYX. /evidence=ECO:0000250\|UniProtKB:Q9UMS6	993..1012		6/42 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1037..1060		6/22 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1703
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900