Protein Alignment Khc-73 and Kif28

DIOPT Version :10

• Sequence 1: NP_001261000.1
• Gene: Khc-73 / 36718
• FlyBaseID: FBgn0019968
• Length: 1957
• Species: Drosophila melanogaster
• Sequence 2: NP_001357826.1
• Gene: Kif28 / 383592
• MGIID: 2686151
• Length: 1033
• Species: Mus musculus
• Alignment Length: 1198
• Identity: 347/1198 - (28%)
• Similarity: 540/1198 - (45%)
• Gaps: 257/1198 - (21%)

  Fly     3 SDKIKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYS----- 62
            ||.::||||||||::||....::|::.|......:::|...|::     |||.||..::|
Mouse    14 SDSVRVAVRVRPFSQREKNSGSQCVISMHSGNISIRDPKNEERV-----KTFTFDLTYWSHDGFQ 73

  Fly    63 ------LNPED--ENFASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGI 119
                  |.|.|  ..||.|..||..:||||||:|::||||.:.|||||||||||:|:|...:||:
Mouse    74 KDEDGVLIPSDPASKFAGQSDVFHDIGRGILDSAWRGYNATLLAYGQTGSGKSYSMIGFGTNKGL 138

  Fly   120 IPRLCDQLFSAIANKS---TPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVD 181
            |||:|::||.||..:.   .|::|:    |.:|||||::.|||........|:|||...:|.:|:
Mouse   139 IPRVCEELFQAIEKQKENLEPQVMF----SMLEIYNEQIRDLLSRTKAPGGLRVREDQQLGFFVE 199

  Fly   182 GLSQLAVTSYQDIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRM 246
            ||..:...:|..|:.|:.:|:|.|..|:|||||.|||||.:.::...|:..|.|.    .|.|.:
Mouse   200 GLKWVPCENYAQIEKLVEQGSKIRMTASTNMNASSSRSHMLIAIQFKQVFLDTAL----TKRSSI 260

  Fly   247 SLVDLAGSERAVKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTW 311
            ::||||||||...:|:.||||:|||.:|.|||:||.|||.|||.:.|||.   ..:||||||||.
Mouse   261 NMVDLAGSERQRSSGSEGDRLREGSRVNLSLTSLGNVISALADLAMGKKV---LHIPYRDSVLTK 322

  Fly   312 LLKDNLGGNSRTVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVET 376
            ||:..|||||||.::|.:||:...|||||||||||:|||::.|.||:|..|.||..|       .
Mouse   323 LLQSALGGNSRTTLIAALSPADICYEETLSTLRYAERAKKVRNRAVINTCPLARASR-------A 380

  Fly   377 LRSMLKHATGSPVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIK 441
            ..::|....|:...:........:.|..|  .||.:.|.:..|...|:.:||.:..        |
Mouse   381 ENALLLGFRGAGAAEHPACFWAEQQLGNQ--GTWAQLLEQARREWEEQYEALTQEQ--------K 435

  Fly   442 VEKNKYYLVNLNADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITIEDSGLY 506
            :.:...:|:|:|.||.|..:|.:::.:.:...||..|..   |.|.||||..:|......|..:.
Mouse   436 MVRILPHLLNVNEDPQLTGVLKFFIHNGSCDVGRAASNA---ICLQGLGISDKHASFVNLDGKVT 497

  Fly   507 MEPVQGARCFVNGSAAVEKTPLQNGDRILWG-NHHFFRVNSPKSNNTSMCASEPQTPAQLIDYNF 570
            :.|....:..|||.....:|.||:.|||:.| |..|..|..|......        .....||:|
Mouse   498 VAPHSKCKVIVNGVPVTGRTKLQHLDRIILGSNSAFLYVGFPSERGAE--------DLSRFDYDF 554

  Fly   571 ARDEIMQNELSNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILSPSTPYAPYAPYD 635
                               .:|||...|...||
Mouse   555 -------------------FQLERAAAEGVSVA-------------------------------- 568

  Fly   636 PLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSV 700
              .:|.::|.               |:....|:..:..|.::...||.|||.::||::|    .:
Mouse   569 --TLGCVSPG---------------DDQADPSILAVFQDYVKLMPLVVEANQMSEELKK----GL 612

  Fly   701 TLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQ---IWTMEKLENKLIDMREMYQEHKERVLNG 762
            |:::...||:.:..|| :..:..::||.|..|:.   ||:..|..|:...|.|:||    |.|..
Mouse   613 TMELKVKNLASSDSRG-YDLQKEVMVKVTKQGTHEVWIWSKAKFINRKFLMEELYQ----RFLES 672

  Fly   763 LDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQ 827
            .|...|:..|||::..|..:| |.|:::|:.|.|.:.|:.....::..|.....|.:.|
Mouse   673 RDSHVAQEDDPFWDPLEVVHL-GSAHVWLQPLAHCMMLEEQVEFLNCDGLEEAVLHIRI------ 730

  Fly   828 MPQDRMCESVSESSGDSRDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQ--- 889
                ..|.....:.|: .|...||::....:|..:             ::.|.|....|..:
Mouse   731 ----TPCSPEGWAHGE-EDMVIDPLELLGKRIDFQ-------------THIVRCLGVKWLKEDGS 777

  Fly   890 ---EMVVPVINAESTAHDQNMVFK-----FEHTQDF-TVTINEEFLEHCIEGALSIEVWGHRSA- 944
               :|...:.:..:|.:.: .|:|     .|.|..| .:..:.|||::.:..||.:::||.:..
Mouse   778 RGIQMGYKIYDLPNTLYTK-PVWKSVNPRIEETVHFAALGASREFLDYLLTNALIVDLWGLQEGC 841

  Fly   945 ---GFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVE-----VTNRNEV 1001
               |.|:.                        .:.|..|.|.:.|...:|.|:     .:|:...
Mouse   842 APLGVSQL------------------------DVLLTGEGHIMVDTKTFSSVKDVSQITSNQVPE 882

  Fly  1002 LTGGIYQLRQGQQ---------RRVNVRVK-PVQNSGTLPIICQSIVNVAIGSVTVRSR------ 1050
            |...:.:|.|..:         |..||.:| .::|:|:.| ..|.:.|.. |:....:|
Mouse   883 LYQKLLKLEQETELLRDVNRALRGENVFLKASLENAGSAP-QAQKLDNPE-GATRTAAREAKQVC 945

  Fly  1051 -LQRPLDSYQEEDLTVLREKWSEALG-----RR----------RQYLDQQIQMLIKKEEKNEQER 1099
             .|...|:.....|.|..:....|.|     ||          |.::.||.|||...|:..|
Mouse   946 AQQASSDAQLARALKVFYQGMGVARGQLLRLRRCRPPEDDQMLRPFVHQQSQMLKDLEDLLE--- 1007

  Fly  1100 ERELSLVHQWVS----LTEERNAVLVPA 1123
                |.:|:..:    :.::|...|:|:
Mouse  1008 ----SSLHKLKADVAFIVKKRKEYLLPS 1031

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Khc-73	NP_001261000.1	KISc_KIF1A_KIF1B	4..359	CDD:276816	172/370 (46%)
		Kinesin_assoc	356..468	CDD:465047	26/111 (23%)
		FHA_KIF13	446..547	CDD:438758	32/101 (32%)
		KIF1B	741..786	CDD:463574	14/44 (32%)
		DUF3694	989..1243	CDD:463599	38/176 (22%)
		CAP_GLY	1785..1850	CDD:460154
Kif28	NP_001357826.1	Motor_domain	15..370	CDD:473979	172/370 (46%)
		FHA_KIF28P	441..538	CDD:438761	32/99 (32%)
		KIF1B	655..693	CDD:463574	13/41 (32%)