DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Khc-73 and Kif13a

DIOPT Version :9

Sequence 1:	NP_001261000.1	Gene:	Khc-73 / 36718	FlyBaseID:	FBgn0019968	Length:	1957	Species:	Drosophila melanogaster
Sequence 2:	NP_001100932.1	Gene:	Kif13a / 308173	RGDID:	1307825	Length:	1689	Species:	Rattus norvegicus

Alignment Length:	1768	Identity:	892/1768 - (50%)
Similarity:	1130/1768 - (63%)	Gaps:	180/1768 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly    67 DEN----FASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGTQESKGIIPRLCDQL 127
            ||:    :|.||.||.|:|.|||:.||||||||||||||||||||::|||..|..|:|||||..|
  Rat     2 DESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGHAEQLGLIPRLCCAL 66

  Fly   128 FSAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQ 192
            |..|:.:......:||||||||||||||.||||||.::||||||||.|:||||||||||||||::
  Rat    67 FQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFE 131

  Fly   193 DIDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERA 257
            ||::||:||||||||||||||.|||||||||::::||.|.|..:|.||||||::||||||||||.
  Rat   132 DIESLMSEGNKSRTVAATNMNEESSRSHAVFNIIITQTLYDLQSGNSGEKVSKVSLVDLAGSERV 196

  Fly   258 VKTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSR 322
            .||||.|:||||||||||||||||||||.||||:.||  |.:||||||||||||||||||||||:
  Rat   197 SKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGK--GKNKFVPYRDSVLTWLLKDNLGGNSQ 259

  Fly   323 TVMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGS 387
            |.|:|||||:||||||||||||||||||||||||||||||||::|||||.|||.||..|..|...
  Rat   260 TSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAM 324

  Fly   388 PVGDVQDKLAESENLMKQISQTWEEKLVKTERIQNERQQALEKMGISVQASGIKVEKNKYYLVNL 452
            ...::::||.|||.|:|:::.||||||.|||.|..|||:.||.||||::.|||||..:|.|||||
  Rat   325 KAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNL 389

  Fly   453 NADPSLNELLVYYLKDRTLIGGRTISGQQPDIQLSGLGIQPEHCVITI-EDSGLYMEPVQGARCF 516
            ||||:|||||||||||.|.:|..|    ..||||.|:|||||||.|.| .|..:.:.|.:.||..
  Rat   390 NADPALNELLVYYLKDHTRVGADT----SQDIQLFGIGIQPEHCEIDIATDGDITLTPKENARSC 450

  Fly   517 VNGSAAVEKTPLQNGDRILWGNHHFFRVNSPKSNN--------------------TSMCASEPQT 561
            |||:.....|.|.:||||||||:||||:|.||...                    .|..:|||
  Rat   451 VNGTLVCSVTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETSATEHDLDAASEASSEP-- 513

  Fly   562 PAQLIDYN--FARDEIMQNEL-SNDPIQTAIARLERQHEEDKQVALEKQRQEYERQFQQLRNILS 623
                 |||  ||:.|::...| ||||:|..:..||:|:.|:|:.|||:||..|||:.:|||..||
  Rat   514 -----DYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRTALEEQRLMYERELEQLRQQLS 573

  Fly   624 PSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDEMFRRSLGQLKTDIMRANSLVQEANFL 688
            |.. ..|.:..|     ::..::.|:|.:|.:||:||||:||:||.:|:..:::||:||:|||||
  Rat   574 PER-QPPSSASD-----RLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFL 632

  Fly   689 AEEMEKKTKFSVTLQIPPANLSPNRRRGAFVSEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQ 753
            ||||.|.|.:.||||||.||||.||:|||.||||||..:|....:|:||:|||||||||||::||
  Rat   633 AEEMGKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQARRKGKSTQVWTIEKLENKLIDMRDLYQ 697

  Fly   754 EHKERV-----LNGLDEDNAKPQDPFYESQENHNLIGVANIFLEVLFHDVKLDYHTPIISQQGEV 813
            |.||.|     |.|      |..|||||:|||||||||||:|||.||.||||.|..|||||||||
  Rat   698 EWKENVPKAKRLCG------KRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEV 756

  Fly   814 AGRLQVEIERIAGQMP----QDRMCESVSESSG----DSRDEYDDPVDPTSNQITCRVTIKCASG 870
            ||||.||:.|:.|.:|    :|...|:.|||..    ||..|....|    .::||||.||.|:|
  Rat   757 AGRLHVEVMRVTGTVPERVSEDDSSENSSESGSLEVVDSSGEVIHRV----KKLTCRVIIKEATG 817

  Fly   871 LPLSLSNFVFCQYTFWGHQEMVV--PVINAE---STAHDQNMVFKFEHTQDFTVTINEEFLEHCI 930
            ||:|||||||||||||...|..|  ||::.:   ..:.|......|.|.:|:.||:.|||||...
  Rat   818 LPISLSNFVFCQYTFWDQYESTVAAPVVDPDVPSPQSKDAQYTVTFSHCKDYVVTVTEEFLEFIS 882

  Fly   931 EGALSIEVWGHRSAGFSKTKGWEVEQQQAKARSLVDRWAELSRKIELWVEIHELNDNGEYSPVEV 995
            :|||::||||||.|| :.:..|||:...||.|:|.|||.|::|:||:|:.|.|||:.|:|:.||:
  Rat   883 DGALAVEVWGHRCAG-NGSPIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGDYAAVEL 946

  Fly   996 TNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIICQSIVNVAIGSVTVRS-RLQRPLDSYQ 1059
            ....:|.|||::|||||..|||.|.|||||:|||||::.::|::|:||.||.|| :|||.|||||
  Rat   947 HQAKDVNTGGVFQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSVSIGCVTARSTKLQRGLDSYQ 1011

  Fly  1060 EEDLTVLREKWSEALGRRRQYLDQQIQMLIKKEEKNEQERERELSLVHQWVSLTEERNAVLVPAP 1124
            ||||..:||:||:||.:||:|||:||:.:..|:||.|.:.|||..||.|||.|||||||||||.|
  Rat  1012 EEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDMEREARLVEQWVGLTEERNAVLVPDP 1076

  Fly  1125 GSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQNTNDEL---SVAGINSILSKEHGHKFYTL 1186
            |||||||||.|.||.|||.|:|||||:||.|||||   |::|   ..:|:||||.||||.:|:.|
  Rat  1077 GSGIPGAPADWVPPPGMETHIPVLFLDLNADDLSA---NEQLVGPHASGVNSILPKEHGSQFFYL 1138

  Fly  1187 QILQHLDKDVCCVASWDSSMHDSQALNRVTEANERVYLILRTTVRLSHPAPMDLVLRKRLSINIK 1251
            .|::|.|.:|...||||||:|||..|||||..|||:|||::|||:|||||.|:||||||::.||.
  Rat  1139 PIIKHSDDEVSATASWDSSVHDSLHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIY 1203

  Fly  1252 KGQTLTDRLKKFRLVRGENAIWQSGVTYEVVSNIPKASEELEDRESLAQLAASGDDCSASDGETY 1316
            ..|:.|..||  |.:...|..:..|||||:|||||||:||:||||:||.|||..::....|||||
  Rat  1204 NKQSFTQSLK--RRISLINICYSCGVTYEIVSNIPKATEEIEDRETLALLAARSENEGTLDGETY 1266

  Fly  1317 IEKYTRGVSAVESILTLDRLRQNVAVKE---LETAHGQPLSMRKTVSVPNFSQIMR-------FD 1371
            ||||||||..||:||:|:||||.|.|||   .:|.|     :|:::|.||...:..       ||
  Rat  1267 IEKYTRGVLQVENILSLERLRQAVTVKEALSTKTRH-----IRRSLSTPNVHNVSSSRPDLSGFD 1326

  Fly  1372 AS----MESLLNVG-RSESFADLNNSALGNKFTPAGHSPAGAGGVIRSRHSFGGKGSSDD----- 1426
            ..    .|:.|:|. .|.|:.|:  :..|   |....||         |.|..|..|.:.
  Rat  1327 EDDKGWPENQLDVSDYSSSYQDV--ACYG---TLPRDSP---------RRSKEGCPSENPHALTI 1377

  Fly  1427 SPGKAFGIASPATSKLLGMRMTTLHEEPLGGHRSLDEEPEDSYSDSEYAAEYEQERQQNKSMATR 1491
            ||.|||....|...|.| |.:...|::.:    :|:..|..|..||| ..|.|.|....|.|..:
  Rat  1378 SPFKAFSPQPPKFFKPL-MPVKEEHKKRM----ALEARPLLSQEDSE-EEENELEAVSRKLMHAQ 1436

  Fly  1492 SRLTASKTMDSFMDVSSHSNQSYLSYTSSANANMKHLTGL-ATLSMSSSTS---SGYGSQAVSCN 1552
            ..:..     .|.|.|.::........||:.|::.....| ..:|.|.:||   |||.|.  |.:
  Rat  1437 PYVPV-----EFADFSVYNASLENREWSSSKADLTDSRALEKAVSRSPTTSSLTSGYFSH--SAS 1494

  Fly  1553 NLSNEDIASMRSMSIDETPDFDRVNSNSPPNRQARVNPFLKDMPKAKIQEQPE-QQAKKLQEAFT 1616
            |.:..|:|...|.|.|:.    .:::.....|:........|...|..||..| ::.....:..|
  Rat  1495 NATLSDMAVPSSDSSDQL----ALSTKEAECREHSGPSLAPDFTPASTQELTEAERGLGKDKTIT 1555

  Fly  1617 HPLEQ----LESSQNAQSDDDECAQLPKNNNNNLDLVNEPKPLSSQ--------TDLEESMS--Q 1667
            .|||:    .:.|.:.||..:|..::|.......:|...|.....|        ..:|.:.:  :
  Rat  1556 VPLEENSALPQGSPSPQSIPEENPRMPCRTALGSELDAGPSKEGHQAREFCPGEVTIEHTTNILE 1620

  Fly  1668 PKSKTEF--ATDNQNGNRSSD----ELSHSSEDLLEGDGIVREELPAGKVVRRKKSNTQPPSNGN 1726
            ..|.|||  .:|.::.:..:|    |.| |..||....|  |:.:|.|           ||..|.
  Rat  1621 DHSFTEFMGVSDGKDFDGLADCSVGEPS-SRTDLTIETG--RKGIPEG-----------PPDAGQ 1671

  Fly  1727 SINNNNNGTTQAP 1739
            ..:...|..|..|
  Rat  1672 LHSKIENDQTLHP 1684

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Khc-73	NP_001261000.1	KISc_KIF1A_KIF1B	4..359	CDD:276816	222/295 (75%)
		KISc	6..359	CDD:214526	222/295 (75%)
		Kinesin_assoc	356..468	CDD:292801	71/111 (64%)
		FHA	446..546	CDD:238017	59/100 (59%)
		KIF1B	741..786	CDD:289208	30/49 (61%)
		DUF3694	<1196..1252	CDD:289256	38/55 (69%)
		CAP_GLY	1786..1850	CDD:279625
Kif13a	NP_001100932.1	Motor_domain	3..296	CDD:277568	221/294 (75%)
		KISc	9..296	CDD:214526	220/288 (76%)
		Kinesin_assoc	293..405	CDD:292801	71/111 (64%)
		FHA	383..480	CDD:238017	59/100 (59%)
		KIF1B	685..729	CDD:289208	30/49 (61%)
		DUF3694	<1148..1207	CDD:289256	38/58 (66%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	329	1.000	Domainoid score	I1125
eggNOG	1	0.900	-	-		E2759_KOG0241
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1479	1.000	Inparanoid score	I100
OMA	1	1.010	-	-		QHG48008
OrthoDB	1	1.010	-	-		D15699at33208
OrthoFinder	1	1.000	-	-		FOG0002517
OrthoInspector	1	1.000	-	-		otm46398
orthoMCL	1	0.900	-	-		OOG6_100247
Panther	1	1.100	-	-	LDO	PTHR24115
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1652
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880