Protein Alignment Pms2 and MLH3

DIOPT Version :10

• Sequence 1: NP_477023.1
• Gene: Pms2 / 36705
• FlyBaseID: FBgn0011660
• Length: 899
• Species: Drosophila melanogaster
• Sequence 2: NP_015161.1
• Gene: MLH3 / 855939
• SGDID: S000006085
• Length: 715
• Species: Saccharomyces cerevisiae
• Alignment Length: 961
• Identity: 199/961 - (20%)
• Similarity: 331/961 - (34%)
• Gaps: 359/961 - (37%)

  Fly    23 LSGQIKAIGKDTVHKICSGQVVLSLAVAVKELVENSIDAGATLVEIKLKDQGLQSVEVSDNGSGV 87
            :|..|:.:..|...::.|....:|||.||:|:|:||:||.||.:::.:....| |..|.|:|.|:
Yeast     1 MSQHIRKLDSDVSERLKSQACTVSLASAVREIVQNSVDAHATTIDVMIDLPNL-SFAVYDDGIGL 64

  Fly    88 EEMNLEGMTAKYHTSKIREFVDLLGVETFGFRGEALSSLCALSDMVIQTRHKSTDVGVKVELDHE 152
            ...:|..:..:.:|||||:..||:.::|:|:||:||.|:..:|::.:.::.|          |:.
Yeast    65 TRSDLNILATQNYTSKIRKMNDLVTMKTYGYRGDALYSISNVSNLFVCSKKK----------DYN 119

  Fly   153 GRIKKRSPCARGV------------------------GTTVLLANLFSTLPVRRRDFTRNIKKE- 192
            ....::.| ::.|                        ||.|::.::...||||||     |.||
Yeast   120 SAWMRKFP-SKSVMLSENTILPIDPFWKICPWSRTKSGTVVIVEDMLYNLPVRRR-----ILKEE 178

  Fly   193 ---------FTKMCQILQAYCLVTKGVRILCSNHTPK---------GAKTIVLQTHGDQEVMANI 239
                     ...|.|||..:.:::..|:     :|.|         .:|.|.......|::...:
Yeast   179 PPFKTFNTIKADMLQILVMHPMISLNVQ-----YTDKLRINTEVLFRSKNITEGLTKHQQMSQVL 238

  Fly   240 SAIFGARQAADLVPLKSPFGQGQLTEAELRADLESGADLADTTSPQISTEDVERLNQADFQLEGF 304
            ..:|||     ::|                      .|:....|          |...::|:||.
Yeast   239 RNVFGA-----IIP----------------------PDMLKKVS----------LKFNEYQIEGI 266

  Fly   305 ISSCRHGAGRSSRDRQFFFVNSRPCDPKNIAKVMNEVYHRYNVQQQPFIYLNIITARSDVDVNLT 369
            ||....|.    :|.||.::|.|                ||              |.|...
Yeast   267 ISKMPVGL----KDLQFIYINGR----------------RY--------------ADSAFQ---- 293

  Fly   370 PDKRQLLINNERILLLALKKSLLDTFGQTPATFQMQNTTIVSMLEPKTNSGKTKFPKESSKDDQH 434
                                      |...:.||.|:.....|...||        |...|..:.
Yeast   294 --------------------------GYVDSLFQAQDFGEKGMSLLKT--------KSVGKPYRS 324

  Fly   435 EEVSEEDVPTTSTQRFMDVLTQWRRTGDTKGTAPSVPVKRRCSES-----EELVTRTLKMQKIHT 494
            ..|...||....|   :|.|.|             .|.|:....|     |.|:.:|::  ...|
Yeast   325 HPVFILDVRCPQT---IDDLLQ-------------DPAKKIVKPSHIRTIEPLIVKTIR--SFLT 371

  Fly   495 FLSQESPNEQSSKCEAESDAASDVDMAKKDKT------QVSLDNSFLNLKELAKESEAYDLLTKP 553
            |....:|::..|..|.       |:.::|..|      |:|..|..||.|.......:|  :.||
Yeast   372 FQGYLTPDKSDSSFEI-------VNCSQKTATLPDSRIQISKRNQVLNSKMKIARINSY--IGKP 427

  Fly   554 Q-NTPRIDCKVLTPIKSRRSIFEFKTDAMALDKQESPA--ELNSQP--PSFTQLSEETDIASDED 613
            . |..||:...:.        :| |...:.:|.|:|..  :|:|:|  ..||:
Yeast   428 AVNGCRINNSTIN--------YE-KIKNIRIDGQKSRLRNKLSSRPYDSGFTE------------ 471

  Fly   614 DHIELPTRIEFDEEMEEGLPSNFSSGEITTSLEEIASSLKAHEQQQRDRRTRTKLQRLRFKTEIN 678
                     ::|           |.|:..|..
Yeast   472 ---------DYD-----------SIGKTITDF--------------------------------- 483

  Fly   679 PNQNTSAEAELQREIDKEDFARMEIIGQFNLGFIIVKLEDD-------LFIVDQHATDEKYNFET 736
                         .|.:...|:.|:|.|.:..||:::..|.       |.:|||||.||:...|
Yeast   484 -------------SISRSVLAKYEVINQVDKKFILIRCLDQSIHNCPLLVLVDQHACDERIRLE- 534

  Fly   737 LQRTTQLEYQRLA-------VPQNLELTAV----NEMVLLNHI-DVFEKNGFKFEVDHEAPATKK 789
                 :|.|..|.       |.::|:...:    .|..|..|. ..|:|.|..:|.......|..
Yeast   535 -----ELFYSLLTEVVTGTFVARDLKDCCIEVDRTEADLFKHYQSEFKKWGIGYETIEGTMETSL 594

  Fly   790 VRLLGKPHSKRWEF-GKEDIDELIFMLQDAPE---------------------------GTICRP 826
            :.:...|.....:: |.:|..::: :||.|.:                           .:.|.|
Yeast   595 LEIKTLPEMLTSKYNGDKDYLKMV-LLQHAHDLKDFKKLPMDLSHFENYTSVDKLYWWKYSSCVP 658

  Fly   827 SRVRAMFASRACRKSVMIGTALSRNTTMRRLITQMGEIEQPWNCPHGRPTM 877
            :....:..|:|||.:||.|..|:|...: .||:::.....|:.|.||||:|
Yeast   659 TVFHEILNSKACRSAVMFGDELTRQECI-ILISKLSRCHNPFECAHGRPSM 708

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pms2	NP_477023.1	mutl	25..376	CDD:273155	86/393 (22%)
		mutL	33..880	CDD:234630	197/951 (21%)
MLH3	NP_015161.1	HATPase_MutL-MLH-PMS-like	12..213	CDD:340403	59/222 (27%)
		mutL	24..713	CDD:234630	195/938 (21%)