Protein Alignment Pms2 and MLH1

DIOPT Version :10

• Sequence 1: NP_477023.1
• Gene: Pms2 / 36705
• FlyBaseID: FBgn0011660
• Length: 899
• Species: Drosophila melanogaster
• Sequence 2: NP_000240.1
• Gene: MLH1 / 4292
• HGNCID: 7127
• Length: 756
• Species: Homo sapiens
• Alignment Length: 900
• Identity: 183/900 - (20%)
• Similarity: 353/900 - (39%)
• Gaps: 216/900 - (24%)

  Fly    23 LSGQIKAIGKDTVHKICSGQVVLSLAVAVKELVENSIDAGATLVEIKLKDQGLQSVEVSDNGSGV 87
            ::|.|:.:.:..|::|.:|:|:...|.|:||::||.:||.:|.:::.:|:.||:.:::.|||:|:
Human     4 VAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGI 68

  Fly    88 EEMNLEGMTAKYHTSKIREFVDLLGVETFGFRGEALSSLCALSDMVIQTRHKSTDVGVKVELDHE 152
            .:.:|:.:..::.|||::.|.||..:.|:|||||||:|:..::.:.|.|:........:.... :
Human    69 RKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYS-D 132

  Fly   153 GRIK-KRSPCARGVGTTVLLANLFSTLPVRRRDFTRNIKKEFTKMCQILQAYCLVTKGVRILCSN 216
            |::| ...|||...||.:.:.:||..:..||:.. :|..:|:.|:.:::..|.:...|:    |.
Human   133 GKLKAPPKPCAGNQGTQITVEDLFYNIATRRKAL-KNPSEEYGKILEVVGRYSVHNAGI----SF 192

  Fly   217 HTPKGAKTIV-LQTHGDQEVMANISAIFGARQAADLVPLKSPFGQGQLTEAELRADLESGADLAD 280
            ...|..:|:. ::|..:...:.||.:|||...:.:|:
Human   193 SVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELI---------------------------- 229

  Fly   281 TTSPQISTEDVERLNQADFQLEGFISSCRHGAGRSSRDRQFFFVNSRPCDPKNIAKVMNEVYHRY 345
                :|..||    ....|::.|:||:..:...:..   ...|:|.|..:..::.|.:..||..|
Human   230 ----EIGCED----KTLAFKMNGYISNANYSVKKCI---FLLFINHRLVESTSLRKAIETVYAAY 283

  Fly   346 NVQQ-QPFIYLNIITARSDVDVNLTPDKRQLLINNERILLLALKKSL-LDTFGQTPATFQMQNTT 408
            ..:. .||:||::..:..:||||:.|.|.::...:|..:|..:::.: ....|...:......|.
Human   284 LPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTL 348

  Fly   409 IVSMLEPKTNSGKTKFPKESSKDDQHEEVSEEDVPTTSTQRFMDVLTQWRRTGDTKGTAPSVPVK 473
            :..:..|                      |.|.|.:|::      ||    :..|.|::..|
Human   349 LPGLAGP----------------------SGEMVKSTTS------LT----SSSTSGSSDKV--- 378

  Fly   474 RRCSESEELVTRTLKMQKIHTFLSQESPNEQSSKCEAESDAASDVDMAKKDKTQVSLDNSFLNLK 538
                .:.::|....:.||:..|| |......||:.:|         :..:|||.:|...:
Human   379 ----YAHQMVRTDSREQKLDAFL-QPLSKPLSSQPQA---------IVTEDKTDISSGRA----- 424

  Fly   539 ELAKESEAYDLLTKPQNTPRIDCKVLTPIKSRRSIFEFKTDAMALDKQESPAELNSQPPSFTQLS 603
                         :.|:...::......:.::....|..|.....:..|.....:|.|..  :..
Human   425 -------------RQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRK--RHR 474

  Fly   604 EETDIASDEDDH--------------IELPTRIEFDEEMEEGLPSNFSSGEITTSLEEIASSLKA 654
            |::|:...|||.              |.|.:.:...||:.|      ...|:...:....|.:..
Human   475 EDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINE------QGHEVLREMLHNHSFVGC 533

  Fly   655 HEQQQRDRRTRTKLQRLRFKTEINPNQNTSAEAELQREIDKEDFARMEIIGQFNLGFIIVKLEDD 719
            ...|....:.:|||..|         ..|....||..:|...|||        |.|.:.:.....
Human   534 VNPQWALAQHQTKLYLL---------NTTKLSEELFYQILIYDFA--------NFGVLRLSEPAP 581

  Fly   720 LFIVDQHATDE----------------KYNFETLQRTTQLEYQRLAVPQNLELTAVNEMVLLNHI 768
            ||.:...|.|.                :|..|.|::..::    ||...:||:.....::
Human   582 LFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEM----LADYFSLEIDEEGNLI----- 637

  Fly   769 DVFEKNGFKFEVDHEAPATK--KVRLLGKPHSKRWEFGKEDIDELIFMLQDAPEGTICRPSRVRA 831
                  |....:|:..|..:  .:.:|.......|:..||..:.|               |:..|
Human   638 ------GLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESL---------------SKECA 681

  Fly   832 MFASRACRKSVMIGTALSRNTTMRRLITQM-GEIEQPWNCPHGRPTMRHLINIAM 885
            ||.|  .||..     :|..:|:....::: |.|...|     :.|:.|::..|:
Human   682 MFYS--IRKQY-----ISEESTLSGQQSEVPGSIPNSW-----KWTVEHIVYKAL 724

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pms2	NP_477023.1	mutl	25..376	CDD:273155	90/353 (25%)
		mutL	33..880	CDD:234630	179/883 (20%)
MLH1	NP_000240.1	mutl	6..315	CDD:273155	90/353 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	355..378		9/54 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	400..491		18/119 (15%)
		Interaction with EXO1. /evidence=ECO:0000269|PubMed:11427529, ECO:0000269|PubMed:11429708, ECO:0000269|PubMed:12414623, ECO:0000269|PubMed:14676842, ECO:0000269|PubMed:22753075	410..650		51/306 (17%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:29175432	471..474		0/4 (0%)
		Mlh1_C	502..756	CDD:465109	54/287 (19%)