DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pms2 and MLH1

DIOPT Version :9

Sequence 1:	NP_477023.1	Gene:	Pms2 / 36705	FlyBaseID:	FBgn0011660	Length:	899	Species:	Drosophila melanogaster
Sequence 2:	NP_000240.1	Gene:	MLH1 / 4292	HGNCID:	7127	Length:	756	Species:	Homo sapiens

Alignment Length:	900	Identity:	183/900 - (20%)
Similarity:	353/900 - (39%)	Gaps:	216/900 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 LSGQIKAIGKDTVHKICSGQVVLSLAVAVKELVENSIDAGATLVEIKLKDQGLQSVEVSDNGSGV 87
            ::|.|:.:.:..|::|.:|:|:...|.|:||::||.:||.:|.:::.:|:.||:.:::.|||:|:
Human     4 VAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGI 68

  Fly    88 EEMNLEGMTAKYHTSKIREFVDLLGVETFGFRGEALSSLCALSDMVIQTRHKSTDVGVKVELDHE 152
            .:.:|:.:..::.|||::.|.||..:.|:|||||||:|:..::.:.|.|:........:.... :
Human    69 RKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYS-D 132

  Fly   153 GRIK-KRSPCARGVGTTVLLANLFSTLPVRRRDFTRNIKKEFTKMCQILQAYCLVTKGVRILCSN 216
            |::| ...|||...||.:.:.:||..:..||:.. :|..:|:.|:.:::..|.:...|:    |.
Human   133 GKLKAPPKPCAGNQGTQITVEDLFYNIATRRKAL-KNPSEEYGKILEVVGRYSVHNAGI----SF 192

  Fly   217 HTPKGAKTIV-LQTHGDQEVMANISAIFGARQAADLVPLKSPFGQGQLTEAELRADLESGADLAD 280
            ...|..:|:. ::|..:...:.||.:|||...:.:|:
Human   193 SVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELI---------------------------- 229

  Fly   281 TTSPQISTEDVERLNQADFQLEGFISSCRHGAGRSSRDRQFFFVNSRPCDPKNIAKVMNEVYHRY 345
                :|..||    ....|::.|:||:..:...:..   ...|:|.|..:..::.|.:..||..|
Human   230 ----EIGCED----KTLAFKMNGYISNANYSVKKCI---FLLFINHRLVESTSLRKAIETVYAAY 283

  Fly   346 NVQQ-QPFIYLNIITARSDVDVNLTPDKRQLLINNERILLLALKKSL-LDTFGQTPATFQMQNTT 408
            ..:. .||:||::..:..:||||:.|.|.::...:|..:|..:::.: ....|...:......|.
Human   284 LPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTL 348

  Fly   409 IVSMLEPKTNSGKTKFPKESSKDDQHEEVSEEDVPTTSTQRFMDVLTQWRRTGDTKGTAPSVPVK 473
            :..:..|                      |.|.|.:|::      ||    :..|.|::..|
Human   349 LPGLAGP----------------------SGEMVKSTTS------LT----SSSTSGSSDKV--- 378

  Fly   474 RRCSESEELVTRTLKMQKIHTFLSQESPNEQSSKCEAESDAASDVDMAKKDKTQVSLDNSFLNLK 538
                .:.::|....:.||:..|| |......||:.:|         :..:|||.:|...:
Human   379 ----YAHQMVRTDSREQKLDAFL-QPLSKPLSSQPQA---------IVTEDKTDISSGRA----- 424

  Fly   539 ELAKESEAYDLLTKPQNTPRIDCKVLTPIKSRRSIFEFKTDAMALDKQESPAELNSQPPSFTQLS 603
                         :.|:...::......:.::....|..|.....:..|.....:|.|..  :..
Human   425 -------------RQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRK--RHR 474

  Fly   604 EETDIASDEDDH--------------IELPTRIEFDEEMEEGLPSNFSSGEITTSLEEIASSLKA 654
            |::|:...|||.              |.|.:.:...||:.|      ...|:...:....|.:..
Human   475 EDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINE------QGHEVLREMLHNHSFVGC 533

  Fly   655 HEQQQRDRRTRTKLQRLRFKTEINPNQNTSAEAELQREIDKEDFARMEIIGQFNLGFIIVKLEDD 719
            ...|....:.:|||..|         ..|....||..:|...|||        |.|.:.:.....
Human   534 VNPQWALAQHQTKLYLL---------NTTKLSEELFYQILIYDFA--------NFGVLRLSEPAP 581

  Fly   720 LFIVDQHATDE----------------KYNFETLQRTTQLEYQRLAVPQNLELTAVNEMVLLNHI 768
            ||.:...|.|.                :|..|.|::..::    ||...:||:.....::
Human   582 LFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEM----LADYFSLEIDEEGNLI----- 637

  Fly   769 DVFEKNGFKFEVDHEAPATK--KVRLLGKPHSKRWEFGKEDIDELIFMLQDAPEGTICRPSRVRA 831
                  |....:|:..|..:  .:.:|.......|:..||..:.|               |:..|
Human   638 ------GLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESL---------------SKECA 681

  Fly   832 MFASRACRKSVMIGTALSRNTTMRRLITQM-GEIEQPWNCPHGRPTMRHLINIAM 885
            ||.|  .||..     :|..:|:....::: |.|...|     :.|:.|::..|:
Human   682 MFYS--IRKQY-----ISEESTLSGQQSEVPGSIPNSW-----KWTVEHIVYKAL 724

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pms2	NP_477023.1	MutL	26..880	CDD:223400	180/890 (20%)
		HATPase_c	46..>151	CDD:294057	34/104 (33%)
		MutL_Trans_hPMS_2_like	238..381	CDD:239566	30/143 (21%)
		MutL_C	703..847	CDD:214857	28/161 (17%)
MLH1	NP_000240.1	mutl	6..315	CDD:273155	90/353 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	355..378		9/54 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	400..491		18/119 (15%)
		Interaction with EXO1. /evidence=ECO:0000269\|PubMed:11427529, ECO:0000269\|PubMed:11429708, ECO:0000269\|PubMed:12414623, ECO:0000269\|PubMed:14676842, ECO:0000269\|PubMed:22753075	410..650		51/306 (17%)
		Mlh1_C	502..756	CDD:318596	54/287 (19%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0323
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D735423at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820