DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-5 and Unc5b

DIOPT Version :9

Sequence 1:	NP_001286450.1	Gene:	unc-5 / 36703	FlyBaseID:	FBgn0034013	Length:	1072	Species:	Drosophila melanogaster
Sequence 2:	NP_071543.2	Gene:	Unc5b / 60630	RGDID:	621756	Length:	946	Species:	Rattus norvegicus

Alignment Length:	1070	Identity:	306/1070 - (28%)
Similarity:	447/1070 - (41%)	Gaps:	259/1070 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    93 SSSGLGSSGAAGGSGILLEEFNSGKLSPGEASNTLPIFLIEPESVFVVKNRPAVLKCKASHSLQV 157
            |.:|:.|.|.|     |.:.|      |...:..||.||:|||..::|||:|..|.|:|..:.|:
  Rat    25 SLAGIDSGGQA-----LPDSF------PSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQI 78

  Fly   158 IFKCSGS--SQPPPSTHETHVDPHTGVNMEEVTATIHRDLVDEFFGDGPFKCECHAWSSRGVVKS 220
            .|||:|.  ||....|.|: :|..||:.:.||...:.|..|:|.||...:.|:|.||||.|..||
  Rat    79 YFKCNGEWVSQKGHVTQES-LDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKS 142

  Fly   221 QAATVHIAYIRKSFNQSPTSLRLELGSRAELRCEPPGGFPEPKLTWHKNNAVITADSEPGITVSA 285
            :.|.:.|||:||:|:|.|.:..:.|.....|:|.||.|.|..::.|.||..||....:....::.
  Rat   143 RRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTI 207

  Fly   286 G-TLIFRQVALQHMANYSCSAENIAGRRVSDSAVLIVYVNGGWSTWSPWREC--KCAGKPSQGRK 347
            . .||.||..|...|||:|.|:||..:|.|.:|.:|||||||||:|:.|..|  :| |:..|  |
  Rat   208 DHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRC-GRGWQ--K 269

  Fly   348 RSRTCNNPMPLNGGAQCPGPQIQKSADCAACPEDTQIVSPDGFDISSSKRMARWSAWSDWSICSA 412
            |:|||.||.||||||.|.|...||:|....||.|                 ..|:.||.||.||.
  Rat   270 RTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVD-----------------GAWTEWSKWSACST 317

  Fly   413 ECIQVRRRKCLTQGQTQISSEAEEAGDLLLGAPGVGMAALIAAAGVGAVGSPSEATGSSSDIIPG 477
            ||...|.|:|:                    ||.                             |.
  Rat   318 ECAHWRSRECM--------------------APP-----------------------------PQ 333

  Fly   478 YGKSLCAGKDIQTAECRGEQCQIGKDDF------------DWTLYLGLA----FITAVCFAFGTA 526
            .|...|:|..:.:..|....|.:.:...            |..||.||.    .:.||..|.|
  Rat   334 NGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVG-- 396

  Fly   527 LICCARRGIRTNPHYNMARSVMDADYMPGVVEKKEMRMHIEASNMGYDYVN-----PG-----HR 581
             :...||..|              |:...:.:..      .|...|:..||     |.     |.
  Rat   397 -VIVYRRNCR--------------DFDTDITDSS------AALTGGFHPVNFKTARPSNPQLLHP 440

  Fly   582 YLPGEHIMGMGIGCGGV---TEHHYDVPNLSANYTNPIDHLSVDYLSETGESSTADTSNSTFDMN 643
            ..|.:.....||..|.|   .:....:|..::...:|:..|.:    :..:|||..:.....|..
  Rat   441 SAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKI----KVYDSSTIGSGAGLADGA 501

  Fly   644 GKLSIL-----------------------------------NASKSSTYEMLGSAGGQLRLYGGE 673
            ..|.:|                                   ::|.|.|:   |..||:|.:.|..
  Rat   502 DLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTF---GCLGGRLTIPGTG 563

  Fly   674 LLLFVPEHAI--GK------HVKKHVSLLLLSDECS-----RVSCATESSILCSSVVHSAPRNYS 725
            :.|.||..||  ||      .:.|..|.|.||:...     .|:|.....:||
  Rat   564 VSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLC------------ 616

  Fly   726 FVKPVILKIPHCL-VAPEQWHVHIYHADSEHDELSVNWRRAVSVGEETINTPMFVQLEATHVFIM 789
              :||:|.:|||. |....|   |:...::..:  .:|...|::.|||:|||.:.||||....|:
  Rat   617 --RPVVLTVPHCAEVIAGDW---IFQLKTQAHQ--GHWEEVVTLDEETLNTPCYCQLEAKSCHIL 674

  Fly   790 TEQLGHFTVVAEPRIQQPSIKMKLLAFSQHTPPSNANC-----SLRIYVVKDFPNSRDICANVEA 849
            .:|||.:....|...:....:::|..|:.      |.|     |||:|.::|.|.:......:|.
  Rat   675 LDQLGTYVFTGESYSRSAVKRLQLAIFAP------ALCTSLEYSLRVYCLEDTPAALKEVLELER 733

  Fly   850 KLGGSFLGESQVFAFTLNSRNLNIRVRSADVEAA-------APYEHAIPYQHILS-NNSILHCEF 906
            .|||..:.|.:...|..:..||.:.:.  |:..|       |.|:. ||:.|:.: :...|||.|
  Rat   734 TLGGYLVEEPKPLLFKDSYHNLRLSLH--DIPHAHWRSKLLAKYQE-IPFYHVWNGSQKALHCTF 795

  Fly   907 SLRRQDQNS------LCVDFGQGSEDDYYTFNIPAHSMSGSAEELASTTNTTISIDRQGNYVNES 965
            :|.|....|      :||...:|....:......|.:.:||.:.|.|..... :..:.|.|.
  Rat   796 TLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNA-ATTQLGPYA--- 856

  Fly   966 CVMDFVQLPHATKRLICGALDPPRADERDWRLLAKKLNTDRYIAYFATKASPTEQILNLWECRAN 1030
                 .::|.:.::.||.:||.|.:...||||||:||:.|||:.|||||||||..||:|||.|..
  Rat   857 -----FKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQ 916

  Fly  1031 SSPGSSSNSVSHTIMALLLTLKEMGRQDVL 1060
            .....:|         |...|:|||:.::|
  Rat   917 DDGDLNS---------LASALEEMGKSEML 937

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-5	NP_001286450.1	I-set	233..321	CDD:254352	30/88 (34%)
		Ig	236..321	CDD:299845	29/85 (34%)
		TSP1	327..379	CDD:214559	26/53 (49%)
		TSP1	401..>422	CDD:214559	11/20 (55%)
		ZU5	655..749	CDD:295340	30/107 (28%)
		Death_UNC5-like	973..1064	CDD:260051	39/88 (44%)
Unc5b	NP_071543.2	Ig	158..244	CDD:416386	29/85 (34%)
		Ig strand A'	162..166	CDD:409353	0/3 (0%)
		Ig strand B	170..177	CDD:409353	2/6 (33%)
		Ig strand C	185..190	CDD:409353	1/4 (25%)
		Ig strand C'	192..195	CDD:409353	0/2 (0%)
		Ig strand D	204..207	CDD:409353	0/2 (0%)
		Ig strand E	209..215	CDD:409353	2/5 (40%)
		Ig strand F	222..230	CDD:409353	5/7 (71%)
		Ig strand G	233..244	CDD:409353	3/10 (30%)
		TSP1	250..301	CDD:214559	26/53 (49%)
		TSP1	306..354	CDD:214559	19/96 (20%)
		ZU5	542..645	CDD:128514	33/124 (27%)
		UPA domain	690..839		38/157 (24%)
		UPA	692..831	CDD:407337	35/147 (24%)
		Interaction with DCC. /evidence=ECO:0000269\|PubMed:10399920	708..726		6/17 (35%)
		Death_UNC5B	859..942	CDD:176780	39/88 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353242
Domainoid	1	1.000	91	1.000	Domainoid score	I7498
eggNOG	1	0.900	-	-		E1_KOG1480
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	391	1.000	Inparanoid score	I1925
OMA	1	1.010	-	-		QHG45590
OrthoDB	1	1.010	-	-		D169564at33208
OrthoFinder	1	1.000	-	-		FOG0000918
OrthoInspector	1	1.000	-	-		otm45956
orthoMCL	1	0.900	-	-		OOG6_102612
Panther	1	1.100	-	-	O	PTHR12582
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X496
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.770