DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-5 and unc5b

DIOPT Version :9

Sequence 1:	NP_001286450.1	Gene:	unc-5 / 36703	FlyBaseID:	FBgn0034013	Length:	1072	Species:	Drosophila melanogaster
Sequence 2:	NP_001104619.1	Gene:	unc5b / 494130	ZFINID:	ZDB-GENE-041213-1	Length:	940	Species:	Danio rerio

Alignment Length:	1045	Identity:	301/1045 - (28%)
Similarity:	449/1045 - (42%)	Gaps:	223/1045 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly   106 SGILLEEFNSGKLSPGEASNTLPIFLIEPESVFVVKNRPAVLKCKASHSLQVIFKCSGS--SQPP 168
            |.:|.:.|.|....|      ||.|..|||..|:|||||..|.|||:.:.|:.|||:|.  :|..
Zfish    35 SEVLPDSFPSAPAEP------LPEFQSEPEDAFIVKNRPVKLSCKAAPATQIYFKCNGEWVNQND 93

  Fly   169 PSTHETHVDPHTGVNMEEVTATIHRDLVDEFFGDGPFKCECHAWSSRGVVKSQAATVHIAYIRKS 233
            ..|.|: :|..||:.:.||..::.|..|:|.||...:.|:|.||||.|..||:.|.|.|||:||:
Zfish    94 HVTKES-LDHITGLVVREVDISVSRTQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKN 157

  Fly   234 FNQSPTSLRLELGSRAELRCEPPGGFPEPKLTWHKNNAVI--TADSEPGITVSAGTLIFRQVALQ 296
            |.|.|....:.|.....|:|.||.|.|..::.|.||..:|  ..||...||:. ..||.:|..|.
Zfish   158 FEQEPLGREVRLEQEVLLQCRPPEGSPPAEVDWLKNEELIDPALDSNFLITIE-HDLIIKQARLS 221

  Fly   297 HMANYSCSAENIAGRRVSDSAVLIVYVNGGWSTWSPWRECKC-AGKPSQGRKRSRTCNNPMPLNG 360
            ..|||:|.|.|:..:|.|.:|.|||||:||||:|:.|.||.. .|:..|  :|:|:|.||.||||
Zfish   222 DTANYTCVARNVVAKRRSSTATLIVYVSGGWSSWTEWSECNAQCGRGWQ--RRTRSCTNPAPLNG 284

  Fly   361 GAQCPGPQIQKSADCAACPEDTQIVSPDGFDISSSKRMARWSAWSDWSICSAECIQVRRRKCLTQ 425
            ||.|.||..|:......||.|                 ..|:.|:.||.|..||...|.|:|
Zfish   285 GAFCDGPPFQRVTCTTLCPVD-----------------GGWTEWAKWSACGTECTHWRSREC--- 329

  Fly   426 GQTQISSEAEEAGDLLLGAPGVGMAALIAAAGVGAVGSPSEATGSSSDIIPGYGKSLCAGKDIQT 490
                             .||.                             |..|...|:|..:::
Zfish   330 -----------------QAPP-----------------------------PRNGGRHCSGSMMES 348

  Fly   491 AECRGEQCQIGKD-DFDWT-----------LYLGLAFITAVCFAFGTALICCA-----RRGIR-T 537
            ..|....|...|. ..:.|           :|.||.....:|    ..|:.|.     ||..| .
Zfish   349 KNCTEGLCARNKKVSVEHTSHPLGSGTGVAVYAGLVGALLLC----VILVLCVGILVYRRSCRHL 409

  Fly   538 NPHYNMARSVMDADYMPGVVEKKEMRMHIEASNMGYDYVNPG--HRYLPGEHIMGMGIGCG---- 596
            :.....:.|.:.|.:.||  ..|..|..           ||.  |...|.:.....|...|
Zfish   410 HGEITDSSSALTAAFHPG--NYKPPRQD-----------NPHLLHPTAPPDLTASAGTFRGPLFS 461

  Fly   597 ---GVTEHHYDVPNLSANYTNPIDHLSVD-YLSETGESSTADTSNSTFDMNGKLSILNA------ 651
               ...:.|:.:|..::...:|:..|.:. |.|.|..|....|...:.|  |::..|..
Zfish   462 LQQATLDSHHKIPMTTSPLLDPLPSLKIKVYNSSTMSSLELPTGLGSVD--GEIMSLKTVGSGPK 524

  Fly   652 -------------SKSSTYEMLGSAGGQLRLYGGELLLFVPEHAIGKHVKKHVSLLLLSDECSRV 703
                         |.|:|   |||.||:|.:....:.|.||...|.:  .|...:.|:.::..:.
Zfish   525 DHHGHTLPREPSHSASAT---LGSLGGRLTIPNTGVGLLVPPGTIPQ--GKFYEMYLIINKWEKT 584

  Fly   704 SCATE-SSILCSSVVHSAPRNYSFVKPVILKIPHCL-VAPEQWHVHIYHADSEHDELSVNWRRAV 766
            :..:: |..:.|.||...|......:||:|.:|||. :.|..|.:.:...:.:.     .|...:
Zfish   585 TLPSDGSQTVLSPVVSCGPSGMLLSRPVVLTLPHCAQLEPPDWTLTLKMQNHQG-----AWEEVL 644

  Fly   767 SVGEETINTPMFVQLEATHVFIMTEQLGHFTVVAEPRIQQPSIK-MKLLAFSQHTPPSNANCSLR 830
            :||||::::|.::|:|.....|:.||||.:.:|.:....:|:.| ::|..|:...|..:.:.|||
Zfish   645 TVGEESLSSPCYLQVEEQSCHILMEQLGTYGLVGQSAPPRPACKRLQLALFAPRAPCLSLDYSLR 709

  Fly   831 IYVVKDFPNSRDICANVEAKLGGSFLGESQVFAFTLNSRNLNIRVRSADVEAAAPYEH------- 888
            :|.::|.|::......||..|||..|.:.:...|..:..||.:.:.  |:    |:.|
Zfish   710 VYCIQDTPHALKEVLEVERSLGGVLLEDPKPLLFKDSYHNLRLSIH--DI----PHSHWRSKLLA 768

  Fly   889 ---AIPYQHILSNNS-ILHCEFSLRR------QDQNSLCVDFGQGSEDDYYTFN------IPAHS 937
               .||:.||.|.:. .|||.|||.|      |....:||...:| |...:..|      :|.||
Zfish   769 KYQEIPFYHIWSGSQRPLHCTFSLERGSLVVSQLTCKICVRQVEG-EGQIFQLNTDIQETLPPHS 832

  Fly   938 ---MSGSAEELASTTNTTISIDRQGNYVNESCVMDFVQLPHATKRLICGALDPPRADERDWRLLA 999
               ..||.          :...:.|.|.        .:||.:.::.||.:||.|.|...|||:||
Zfish   833 PLPAGGSC----------LPSSQVGPYA--------FRLPVSIRQKICASLDAPSARGCDWRMLA 879

  Fly  1000 KKLNTDRYIAYFATKASPTEQILNLWE-CRANSSPGSSSNSVSHTIMALLLTLKEMGRQDVLDII 1063
            :.|..|||:.|||||.|||..:|:||| |....:          .:::|...|:|||:.:|| ::
Zfish   880 RSLAFDRYLNYFATKPSPTGVLLDLWEACHQGDA----------DLVSLATALEEMGKSEVL-VV 933

  Fly  1064 VETIG 1068
            :.|.|
Zfish   934 MTTDG 938

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-5	NP_001286450.1	I-set	233..321	CDD:254352	32/89 (36%)
		Ig	236..321	CDD:299845	31/86 (36%)
		TSP1	327..379	CDD:214559	23/52 (44%)
		TSP1	401..>422	CDD:214559	9/20 (45%)
		ZU5	655..749	CDD:295340	26/95 (27%)
		Death_UNC5-like	973..1064	CDD:260051	37/91 (41%)
unc5b	NP_001104619.1	I-set	158..246	CDD:254352	32/88 (36%)
		Ig	160..246	CDD:299845	31/86 (36%)
		TSP1	252..303	CDD:214559	23/52 (44%)
		TSP1	308..356	CDD:214559	17/96 (18%)
		ZU5	538..632	CDD:279170	28/98 (29%)
		Death_UNC5B	853..936	CDD:176780	37/93 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170595330
Domainoid	1	1.000	65	1.000	Domainoid score	I10013
eggNOG	1	0.900	-	-		E1_KOG1480
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	381	1.000	Inparanoid score	I2014
OMA	1	1.010	-	-		QHG45590
OrthoDB	1	1.010	-	-		D169564at33208
OrthoFinder	1	1.000	-	-		FOG0000918
OrthoInspector	1	1.000	-	-		otm25359
orthoMCL	1	0.900	-	-		OOG6_102612
Panther	1	1.100	-	-	O	PTHR12582
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4816
SonicParanoid	1	1.000	-	-		X496
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800