DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-5 and Unc5d

DIOPT Version :9

Sequence 1:	NP_001286450.1	Gene:	unc-5 / 36703	FlyBaseID:	FBgn0034013	Length:	1072	Species:	Drosophila melanogaster
Sequence 2:	NP_001100789.2	Gene:	Unc5d / 306534	RGDID:	1309245	Length:	956	Species:	Rattus norvegicus

Alignment Length:	1051	Identity:	275/1051 - (26%)
Similarity:	420/1051 - (39%)	Gaps:	238/1051 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    99 SSGAAGGSGILLEEFNSGKLSPG---EASNTLPIFLIEPESVFVVKNRPAVLKCKASHSLQVIFK 160
            ::||....|.     :||::.|.   .|..|||.|:.|||..:::|:.|..|:|||..::|:.||
  Rat    25 AAGAEAARGA-----DSGEVLPDSIPSAPGTLPHFIEEPEDAYIIKSNPIALRCKARPAMQIFFK 84

  Fly   161 CSGS-SQPPPSTHETHVDPHTGVNMEEVTATIHRDLVDEFFGDGPFKCECHAWSSRGVVKSQAAT 224
            |:|. ........|..:|..:|:.:.||...:.|..|::|.|...:.|:|.|||..|..||:.|:
  Rat    85 CNGEWVHQNEHVSEESLDESSGLKVREVFINVTRQQVEDFHGPEDYWCQCVAWSHLGTSKSRKAS 149

  Fly   225 VHIAYIRKSFNQSPTSLRLELGSRAELRCEPPGGFPEPKLTWHKNNAVITADSEPGITVSAG-TL 288
            |.|||:||:|.|.|....:.:.....|.|.||.|.|..::.|.||...|.::.:..|...|. .|
  Rat   150 VRIAYLRKNFEQDPQGREVPIEGMIVLHCRPPEGVPAAEVEWLKNEEPIDSEQDENIDTRADHNL 214

  Fly   289 IFRQVALQHMANYSCSAENIAGRRVSDSAVLIVYVNGGWSTWSPWREC--KCAGKPSQGR---KR 348
            |.||..|....||:|.|.||..:|.|.||.::||||||||:|:.|..|  :|      ||   ||
  Rat   215 IIRQARLSDSGNYTCMAANIVAKRRSLSATVVVYVNGGWSSWTEWSACNVRC------GRGWQKR 273

  Fly   349 SRTCNNPMPLNGGAQCPGPQIQKSADCAACPEDTQIVSPDGFDISSSKRMARWSAWSDWSICSAE 413
            ||||.||.||||||.|.|..:||....|.||.|                 ..|..||:||:||.|
  Rat   274 SRTCTNPAPLNGGAFCEGMSVQKITCTALCPVD-----------------GSWEVWSEWSVCSPE 321

  Fly   414 CIQVRRRKCLTQGQTQISSEAEEAGDLLLGAPGVGMAALIAAAGVGAVGSPSEATGSSSDIIPGY 478
            |..:|.|:|.                    ||.                             |..
  Rat   322 CEHLRIRECT--------------------APP-----------------------------PRN 337

  Fly   479 GKSLCAGKDIQTAECRGEQCQIGKDDF-----------------DWTLY--LGLAFITAVCFAFG 524
            |...|.|...::..|....|.:.|...                 |..||  ||.|.:.......|
  Rat   338 GGKFCEGLSQESENCTDGLCILDKKPLHEIKPQRWSRRGIENASDIALYSGLGAAVVAVAVLVIG 402

  Fly   525 TALICCARRGIRTNPHYNMARSVMDADYMPGVVEKKEMRMHIEASNMGYDYVNPGHRYL--PGEH 587
            ..|             |..:.|    ||...|::...:....:..|  :..|..|:..|  |..|
  Rat   403 VTL-------------YRRSHS----DYGVDVIDSSALTGGFQTFN--FKTVRQGNSLLLNPAMH 448

  Fly   588 IMGMGIGCGGVTEHHYDVPNL--SANYTNPI---DHLSVDYLSET-------------------- 627
                              |:|  |..|:.||   |.|..:.::|:
  Rat   449 ------------------PDLTVSRTYSGPICLQDPLDKELMTESSLFNPLSDIKVKVQSSFMVS 495

  Fly   628 ----------GES-------------STADTSNSTFDMNGKLSILNASKSSTYEMLGSAGGQLRL 669
                      |::             ||....|.|..:....|:...::..|..:.|..||:|.:
  Rat   496 LGVSERAEYHGKNHSGTFPHGNNRGFSTIHPRNKTPYIQNLSSLPTRTELRTTGVFGHLGGRLVM 560

  Fly   670 YGGELLLFVPEHAIGKHVKKHVSLLLLSDECSRVSCATESSILCSSVVHSAPRNYSFVKPVILKI 734
            ....:.|.:|..||.:.....:.:.:...|.|..|..:|  :|.|..|...|.:.....|..|.|
  Rat   561 PNTGVSLLIPHGAIPEENSWEIYMSINQGEPSLQSDGSE--VLLSPEVTCGPPDMLVTTPFALTI 623

  Fly   735 PHCL-VAPEQWHVHIYHADSEHDELSVNWRRAVSVGEETINTPMFVQLEATHVFIMTEQLGHFTV 798
            |||. |:.|.|::|:.....:.     .|...:||.:|:.:....:...|.||.:  :..|.:.:
  Rat   624 PHCADVSSEHWNIHLKKRTQQG-----KWEEVMSVEDESTSCYCLLDPFACHVLL--DSFGTYAL 681

  Fly   799 VAEPRIQQPSIKMKLLAFSQHTPPSNANCSLRIYVVKDFPNSRDICANVEAKLGGSFLGESQVFA 863
            ..|| |...::|...:|.......::.:.:||:|.|.:.|.:.....:.|...||..|.|.::..
  Rat   682 TGEP-ITDCAVKQLKVAVFGCMSCNSLDYNLRVYCVDNTPCAFQEVVSDERHQGGQLLEEPKLLH 745

  Fly   864 FTLNSRNLNIRVRSADVE----AAAPYE--HAIPYQHILSNN-SILHCEFSLRR------QDQNS 915
            |..|:.:|.|.|  .|:.    ...|:.  ..:|:..:.|:| ..|||.|||.|      |....
  Rat   746 FKGNTFSLQISV--LDIPPFLWRIKPFTACQEVPFSRVWSSNRQPLHCAFSLERYTPTTTQLSCK 808

  Fly   916 LCVDFGQGSEDDYYTFNIPAHSMSGSAEELASTTNTTISIDRQGNYVNESCVMDFVQLPHATKRL 980
            :|:...:|.|.   ...:....:....|.:      |........:..::....| ::|::.::.
  Rat   809 ICIRQLKGHEQ---ILQVQTSILESERETI------TFFAQEDSTFPAQTGPKAF-KIPYSIRQR 863

  Fly   981 ICGALDPPRADERDWRLLAKKLNTDRYIAYFATKASPTEQILNLWECRANSSPGSSSNSVSHTIM 1045
            ||...|.|.|..:||::||:|.:.:|.::||||::||:..||||||.|........|
  Rat   864 ICATFDTPNAKGKDWQMLAQKNSINRNLSYFATQSSPSAVILNLWEARHQQDGDLDS-------- 920

  Fly  1046 ALLLTLKEMGR 1056
             |...|:|:||
  Rat   921 -LACALEEIGR 930

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-5	NP_001286450.1	I-set	233..321	CDD:254352	30/88 (34%)
		Ig	236..321	CDD:299845	29/85 (34%)
		TSP1	327..379	CDD:214559	27/56 (48%)
		TSP1	401..>422	CDD:214559	11/20 (55%)
		ZU5	655..749	CDD:295340	27/94 (29%)
		Death_UNC5-like	973..1064	CDD:260051	31/84 (37%)
Unc5d	NP_001100789.2	Ig	51..153	CDD:299845	36/101 (36%)
		Important for interaction with FLRT2. /evidence=ECO:0000269\|PubMed:25374360	89..91		0/1 (0%)
		I-set	159..247	CDD:254352	30/87 (34%)
		Ig	161..247	CDD:299845	29/85 (34%)
		TSP1	253..304	CDD:214559	27/56 (48%)
		TSP1	309..357	CDD:214559	19/96 (20%)
		ZU5	545..640	CDD:279170	27/96 (28%)
		Death_UNC5D	856..953	CDD:176779	31/84 (37%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353243
Domainoid	1	1.000	91	1.000	Domainoid score	I7498
eggNOG	1	0.900	-	-		E1_KOG1480
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	391	1.000	Inparanoid score	I1925
OMA	1	1.010	-	-		QHG45590
OrthoDB	1	1.010	-	-		D169564at33208
OrthoFinder	1	1.000	-	-		FOG0000918
OrthoInspector	1	1.000	-	-		otm45956
orthoMCL	1	0.900	-	-		OOG6_102612
Panther	1	1.100	-	-	O	PTHR12582
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X496
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.770