DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Spred and spred2a

DIOPT Version :9

Sequence 1:	NP_001260979.1	Gene:	Spred / 36643	FlyBaseID:	FBgn0020767	Length:	519	Species:	Drosophila melanogaster
Sequence 2:	XP_686737.2	Gene:	spred2a / 558432	ZFINID:	ZDB-GENE-110830-2	Length:	427	Species:	Danio rerio

Alignment Length:	482	Identity:	153/482 - (31%)
Similarity:	214/482 - (44%)	Gaps:	122/482 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 EGHESD--FLVTVRAQVMTRDESTEGWLPLAGGGLANVSIRKRARLSP----LASGHDYIIYGQR 61
            |.|..|  ::|.|:|.|||||||:.|||...||||:.|.:   .::.|    |...:.::|:|:|
Zfish     4 ESHPDDDSYIVRVKAVVMTRDESSGGWLAQEGGGLSRVGV---CKVVPADLELLGRNGFLIFGER 65

  Fly    62 ISDQSVILSCVINRDLKYYKVMPTFHHWRAGKQRNGLTFQTAADARAFDKGVLRAYNELIDGLAK 126
            :.|:.:||.|.:.:||.|.|..|||||||...::.||:||:.|||||||:||.:|..:|.:|...
Zfish    66 LKDKQMILQCFLKKDLVYTKATPTFHHWRVDNRKCGLSFQSPADARAFDRGVRKAIEDLTEGSTT 130

  Fly   127 SNPTIICPPLTKYDSVGEDDVFMTLDLPVESESLQKIHSSPEGSEKSHKSVSNNSDSEKLPPPPI 191
            |:.|     |.....:|:||||..   ..:|.|     :|.:..|.|.::::..|.||     |.
Zfish   131 SSST-----LQNEAELGDDDVFTN---ATDSSS-----NSSQKRETSMQTLAPISFSE-----PH 177

  Fly   192 HYI---------STDKTSATTSPP-----------DAPPASAAPSPATAGQIAASENYSYVTLTA 236
            |.|         .:|:.....:.|           :........:|.....:...|:|.:.|...
Zfish   178 HCILGHLYDQHRHSDRYYLEQTVPMFQCSRHVRFHEEEEEIVRINPRERSWLTGYEDYRHATTAT 242

  Fly   237 -------------VH--------HDYNYPVVDQPVGAQVLNARRESISALKKRNALEAAQAMAAA 280
                         ||        |||.|                                    :
Zfish   243 RTKPLRPDVTDAYVHLAKSEPPKHDYTY------------------------------------S 271

  Fly   281 QTAAGGGLACRDG----GSGKPLHKP----------NVSDILKKETRLRCRYCHELYSEDFNRRG 331
            ...:|.|...|||    |.|....:|          .|.|   ...||||.||..::|...|.||
Zfish   272 YPLSGDGHVSRDGKTGIGGGVVTGQPRALTAKWLPRRVED---SRERLRCVYCQNMFSPSENGRG 333

  Fly   332 ACEYAPDAFRSGYECISGMGCARCMIYHCMSDAEGETAQHPCDCSASEAGCSKRWLGLAILSLFV 396
            .|:.|||..::....:|.|.||..::||||||.||:.:. ||.|.|||.....|||.|..|||..
Zfish   334 RCQEAPDPVQTCIRRVSFMWCADSLLYHCMSDPEGDYSD-PCSCDASEERFCLRWLALLGLSLLA 397

  Fly   397 PCLWCYPPLRACHLAGIHCGLCGGQHK 423
            ||:.||.|||||:..|:.|..|||:||
Zfish   398 PCMCCYVPLRACYHCGVACHCCGGKHK 424

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Spred	NP_001260979.1	EVH1_SPRED-like	9..122	CDD:269978	53/116 (46%)
Spred	NP_001260979.1	Sprouty	315..413	CDD:282994	46/97 (47%)
spred2a	XP_686737.2	EVH1_SPRED-like	12..126	CDD:269978	53/116 (46%)
spred2a	XP_686737.2	Sprouty	317..414	CDD:282994	46/97 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170575157
Domainoid	1	1.000	115	1.000	Domainoid score	I5969
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	250	1.000	Inparanoid score	I3218
OMA	1	1.010	-	-		QHG45705
OrthoDB	1	1.010	-	-		D331423at33208
OrthoFinder	1	1.000	-	-		FOG0002271
OrthoInspector	1	1.000	-	-		otm24224
orthoMCL	1	0.900	-	-		OOG6_108509
Panther	1	1.100	-	-	O	PTHR11202
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1007
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.910