Protein Alignment Polr1A and RPO21

DIOPT Version :10

• Sequence 1: NP_523743.1
• Gene: Polr1A / 36617
• FlyBaseID: FBgn0019938
• Length: 1642
• Species: Drosophila melanogaster
• Sequence 2: NP_010141.1
• Gene: RPO21 / 851415
• SGDID: S000002299
• Length: 1733
• Species: Saccharomyces cerevisiae
• Alignment Length: 1721
• Identity: 487/1721 - (28%)
• Similarity: 776/1721 - (45%)
• Gaps: 389/1721 - (22%)

  Fly    15 DLEFAVFTDQEIRKLSVVKVITGITFD-ALGHAIPGGLYDIRMGSYGRCMDPCGTCLK-LQDCPG 77
            :::|.:|:.:|:|.:||.|:....|.| ....|..|||.|.|:||..|.: .|.||.: :.:|||
Yeast    16 EVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNL-KCQTCQEGMNECPG 79

  Fly    78 HMGHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAGYIIEAQELELF 142
            |.|||:|..||::..||..::::....|:||.||.:.:|.                      ||.
Yeast    80 HFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHN----------------------ELM 122

  Fly   143 KSEIVCQNTENLVAIKNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSCSLRTAITHSALQRLGKKC 207
            :..:..::::.            ..||::.|.:         ||..|...........|.:.:
Yeast   123 RQALAIKDSKK------------RFAAIWTLCK---------TKMVCETDVPSEDDPTQLVSR-- 164

  Fly   208 RHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFADECRRYLRQIYANYPELLKL 272
            ..|..:...:|....:||     ...|:...||.....:.:|:..:|.....:.|
Yeast   165 GGCGNTQPTIRKDGLKLV-----GSWKKDRATGDADEPELRVLSTEEILNIFKHI---------- 214

  Fly   273 LVPVLGLSNTDLTQGDRSPV----DLFFMDTLPVTPPRARPLNMVGDMLKGNPQTDIYINIIENN 333
                   |..|.|....:.|    :...:..|||.||..||.....:..:|  :.|:...:.:
Yeast   215 -------SVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRG--EDDLTFKLAD-- 268

  Fly   334 HVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETA-HEKLYTAWLALQMSVDVLLDVNMSRE----M 393
                 :||            |..:.:||:...| |..:..|...||..|...:|.:::.:    .
Yeast   269 -----ILK------------ANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQ 316

  Fly   394 KSGEGLKQI---IEKKSGLIRSHMMGKRVNYAARTVITPDPNINVDEIGIPDIFAKKLSYPVPVT 455
            |||..:|.|   ::.|.|.||.::|||||:::|||||:.|||:.:|::|:|...||.|:||..||
Yeast   317 KSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVT 381

  Fly   456 EWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRESLAKLLLSNPKDGIKIVHRHVL 520
            .:|:..|.::|.|||:.||||.|:...:|....:   ..|||....:|     :.|.| |.||::
Yeast   382 PYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDL---RYSKRAGDIQL-----QYGWK-VERHIM 437

  Fly   521 NGDVLLLNRQPSLHKPSIMGHKARILHGEKTFRLHYSNCKAYNADFDGDEMNAHYPQSEVARAEA 585
            :.|.:|.||||||||.|:|.|:.::: ...||||:.|....|||||||||||.|.||||..|||.
Yeast   438 DNDPVLFNRQPSLHKMSMMAHRVKVI-PYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAEL 501

  Fly   586 YNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGRFFNREDYQQLVF-----QGLSQLKKDI 645
            ..|..|....:.|:...|..|::||.:....||::|..|...:....:::     .|
Yeast   502 SQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDG-------- 558

  Fly   646 KLLP-PTILKPAVLWSGKQILSTIIINIIPEGYERINLDSFAKIAGKNWNVSRPRPPICGT---N 706
             ::| |.|:||..|||||||||.    .||.|   |:|..|.:                ||   :
Yeast   559 -VIPTPAIIKPKPLWSGKQILSV----AIPNG---IHLQRFDE----------------GTTLLS 599

  Fly   707 PEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGLIHCMYELYGGDVSTLLLTAFTKVFTFFLQL 771
            |:.|.:     .|.:|:::.||::|:..|::..||||.:....|..|...|.....||..|:|..
Yeast   600 PKDNGM-----LIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLH 659

  Fly   772 EGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVAAALELEDEPPHDELVEK----MEAAYVKD 832
            .||:.|:.|.:......|:..:.|.|.:.       ..|::..|...:.|..|    :..:: :|
Yeast   660 NGFSTGIGDTIADGPTMREITETIAEAKK-------KVLDVTKEAQANLLTAKHGMTLRESF-ED 716

  Fly   833 SKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNLQLMVLSGAKGSMVNTMQISCLLGQI 897
            :..|.|.:.:.|:   |...::|...|          ||::.||::|:|||.:|..|:|..:||.
Yeast   717 NVVRFLNEARDKA---GRLAEVNLKDL----------NNVKQMVMAGSKGSFINIAQMSACVGQQ 768

  Fly   898 ELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGIQPQDFFFHCMAGREGLIDTAVKTSR 962
            .:||||.......::||.|:..:.||:|.||::..::.|:.||:||||.|.||||||||||||:.
Yeast   769 SVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAE 833

  Fly   963 SGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDGLD---ILKSKF---------FNDKFC 1015
            :||:||.|:|.||.:.||||.|.|:|..:|:||:|||||:|   |.|...         |..::.
Yeast   834 TGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYR 898

  Fly  1016 ADFLT----------QNATAILRPAQLQLMKDEE--QLAKVQRHEKHI-----RSWEKKKPAKLR 1063
            .|.|.          ::.:.||...:||::.|||  ||.|.::..:.:     .:|  ..|..:|
Yeast   899 VDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANW--PLPVNIR 961

  Fly  1064 AAFTHFSEELREEVEVKRPNEINSK---TGRRRFDEGLLKLWKKADAEDKA----------LYRK 1115
            ....:..:..  .::..:|:::..|   .|.:...|.||.|..|.:....|          |.|.
Yeast   962 RIIQNAQQTF--HIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRS 1024

  Fly  1116 KYARCPDPTVAVYKQDLYYGSVSERTRKLITDYAKRKPALKETIADIMRVKTIKSLAAPGEPVGL 1180
            :.|                      ||:::.:|...|.|....:::| ..:.::|:..|||.||:
Yeast  1025 RLA----------------------TRRVLQEYRLTKQAFDWVLSNI-EAQFLRSVVHPGEMVGV 1066

  Fly  1181 IAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPG---QQHQ 1242
            :||||||||:||||||||||||.....||.|:|||:|||.:| .|:||||:.:.::||   .|.|
Yeast  1067 LAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVA-KNMKTPSLTVYLEPGHAADQEQ 1130

  Fly  1243 AEKLR-----INLNSVTLANLLEYVHVSTGLTLDPERSYEYDMRFQFLPREVYKEDYGVRPKHII 1302
            |:.:|     ..|.|||:|:.: |.......|:.||......:.|..|..|.
Yeast  1131 AKLIRSAIEHTTLKSVTIASEI-YYDPDPRSTVIPEDEEIIQLHFSLLDEEA------------- 1181

  Fly  1303 KYMHQTFFKQ---LIRAILKVSNASRTTKIVVIDDKKDADKEDDNDLDNGDEVGRSKAKANDDDS 1364
               .|:|.:|   |:|  |::..|:.                :|.||..| :||....:...:|.
Yeast  1182 ---EQSFDQQSPWLLR--LELDRAAM----------------NDKDLTMG-QVGERIKQTFKNDL 1224

  Fly  1365 ----SDDNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDANDDDDEAEDEDDEEKGQDGNDNDGD 1425
                |:|||:.    :.::.|....|..|...:.||.|
Yeast  1225 FVIWSEDNDEK----LIIRCRVVRPKSLDAETEAEEDH--------------------------- 1258

  Fly  1426 DKAVERLLSNDMVKAYTYDKENHLWCQVKLNLSVRYQKPDLTSIIRELAGKSVVHQVQHIKRAII 1490
               :.:.:.|.|::                |:::|                    .|::|:|.::
Yeast  1259 ---MLKKIENTMLE----------------NITLR--------------------GVENIERVVM 1284

  Fly  1491 YK------------GTDDDQLLKTDGINIGEMFQHNKILDLNRLYSNDIHAIARTYGIEAASQVI 1543
            .|            ..:.:.:|:|||:|:.|:.....| |..|:|:|....|....||||....:
Yeast  1285 MKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGI-DPTRIYTNSFIDIMEVLGIEAGRAAL 1348

  Fly  1544 VKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGMEHSSS-PLQQMSFESSLQFLKSAA 1607
            .|||.||....|..|:.||::|:.|.||..|....::|.|...|:: .|.:.|||.:::.|..|.
Yeast  1349 YKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAG 1413

  Fly  1608 GFGRADELSSPSSRLMVGLPVRNGTGAFELL 1638
            .....|:....|..:::|.....|||||:::
Yeast  1414 ASAELDDCRGVSENVILGQMAPIGTGAFDVM 1444

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr1A	NP_523743.1	RNAP_I_RPA1_N	18..982	CDD:259844	299/990 (30%)
		HMG-box_SF	1065..1119	CDD:438789	11/66 (17%)
		RNAP_I_Rpa1_C	1166..1638	CDD:132722	138/499 (28%)
RPO21	NP_010141.1	RNAP_II_RPB1_N	15..853	CDD:259848	299/993 (30%)
		RNA_pol_Rpb1_6	873..1056	CDD:461511	37/209 (18%)
		RNAP_II_Rpb1_C	1035..1446	CDD:132720	142/519 (27%)
		RNA_pol_Rpb1_R	1698..1709	CDD:461513