Protein Alignment Polr1A and POLR1A

DIOPT Version :10

• Sequence 1: NP_523743.1
• Gene: Polr1A / 36617
• FlyBaseID: FBgn0019938
• Length: 1642
• Species: Drosophila melanogaster
• Sequence 2: NP_056240.2
• Gene: POLR1A / 25885
• HGNCID: 17264
• Length: 1720
• Species: Homo sapiens
• Alignment Length: 1744
• Identity: 757/1744 - (43%)
• Similarity: 1052/1744 - (60%)
• Gaps: 165/1744 - (9%)

  Fly    16 LEFAVFTDQEIRKLSVVKVITGITFDALGHAIPGGLYDIRMGSYGRCMDPCGTCLK-LQDCPGHM 79
            :.|.:::.:|::||||..:......|:||:....||||:.:|. ....:.|.||:: ..:|.||:
Human    15 ISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGP-ADSKEVCSTCVQDFSNCSGHL 78

  Fly    80 GHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAG-----YIIE---- 135
            |||||...||||.....:..||...||:|:.|........:::.|||:::.|     |.:|
Human    79 GHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILN 143

  Fly   136 --------------AQELELFKSEIVCQNTENLVAIKNGDMVHPHIAAMYKLLEKNEKNSSNSTK 186
                          .:|||.:.:||| ||  ||:..:..     |:           ||...|..
Human   144 RFLEENPDPSASEIREELEQYTTEIV-QN--NLLGSQGA-----HV-----------KNVCESKS 189

  Fly   187 TSCSLRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVG------TGAETG- 244
            ...:|......:|     |:|.||......||..|...:.....|.:....|      .|.|..
Human   190 KLIALFWKAHMNA-----KRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQ 249

  Fly   245 -GQNKVIFADECRRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRAR 308
             |:...:.....|.:|..::.|....|..|..  |:.:..:.  .|....:||:|.|.|.|.|.|
Human   250 IGKRGYLTPTSAREHLSALWKNEGFFLNYLFS--GMDDDGME--SRFNPSVFFLDFLVVPPSRYR 310

  Fly   309 PLNMVGDMLKGNPQTDIYINIIENNHVLNVVLKYMKGGQEKLTEEA----------------KAA 357
            |::.:||.:..|.|| :.:..:..:.||...|..:...::||.||.                ::.
Human   311 PVSRLGDQMFTNGQT-VNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSF 374

  Fly   358 YQTLKGETAHEKLYTAWLALQMSVDVLLDVNMSR-EMKSGEGLKQIIEKKSGLIRSHMMGKRVNY 421
            ..||.|::..:|||..|:.||..|:::.|..|.: .|....|::||:|||.||.|.|||||||:|
Human   375 LSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDY 439

  Fly   422 AARTVITPDPNINVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFT 486
            |||:||.||..||.:|||||.:||.||:||.|||.|||.|||:.|:|||:|||||:.:.:::|..
Human   440 AARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSR 504

  Fly   487 TYIPADNASKRESLAKLLLS----NPK-DGIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARIL 546
            |.:.|.:.::||::||.||:    .|| .|.|||.|||.|||:|||||||:||:|||..|:||||
Human   505 TALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL 569

  Fly   547 HGEKTFRLHYSNCKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDH 611
            ..||..||||:|||||||||||||||||:||||:.|||||.|......|||||||.||.||||||
Human   570 PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDH 634

  Fly   612 VISGVKLSIRGRFFNREDYQQLVFQGLSQLKKDIKLLPPTILKPAVLWSGKQILSTIIINIIPEG 676
            ::||..::.||.||.||.|.:||::||:.....:|||.|:||||..||:|||::||::||||||.
Human   635 MVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPED 699

  Fly   677 YERINLDSFAKIAGKNWNVSRPRPPICGTNPEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGL 741
            :..:||...|||.||.|....|| .:.|.||:  .:.||||.||.||||.|||||..||::.|||
Human   700 HIPLNLSGKAKITGKAWVKETPR-SVPGFNPD--SMCESQVIIREGELLCGVLDKAHYGSSAYGL 761

  Fly   742 IHCMYELYGGDVSTLLLTAFTKVFTFFLQL-EGFTLGVKDILVTDVADRKRRKIIRECRNVGNSA 805
            :||.||:|||:.|..:||...::||.:||| .||||||:||||...||.||::||.|..:.|..|
Human   762 VHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQA 826

  Fly   806 VAAALELEDEPPHDELVEKMEAAYV-KDSKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPS 869
            |.|||.|.:...:||:..|.:.|:: ||.:...::|.|:|..::.|:|:||..|:|.||..:||.
Human   827 VRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPE 891

  Fly   870 NNLQLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFM 934
            |:||:||.||||||.|||||||||||||||||:|||||.||||||.|..:|.:|::|||:.|||:
Human   892 NSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFL 956

  Fly   935 TGIQPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGE 999
            |||:|.:||||||||||||:|||||||||||||||:|||||||.|.|||||||||.|||||||||
Human   957 TGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGE 1021

  Fly  1000 DGLDILKSKFFNDK-FCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWEKKKPAKL- 1062
            |||||.|::|...| |  .||..|...|::...|..:.......|...|.:.|:.|:.|.|..|
Human  1022 DGLDIPKTQFLQPKQF--PFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLL 1084

  Fly  1063 -RAAFTHFSEELREEVEVKRPNEINSKTGRRRFDEGLLKLWKKADAEDKALYRKKYARCPDPTVA 1126
             |.||..:|::::|.|:..: .|..::.||....:.:|::|.:.|.|.:..|:||.|.||||:::
Human  1085 RRGAFLSYSQKIQEAVKALK-LESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLS 1148

  Fly  1127 VYKQDLYYGSVSERTRKLITDYAKRKPALKE-----------TIADIMRVKTIKSLAAPGEPVGL 1180
            |::.|:|:.||||.....:.||::...|..|           .:..::::|..:||..|||.|||
Human  1149 VWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGL 1213

  Fly  1181 IAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPGQQ--HQA 1243
            :|||||||||||||||||||||||||||||||||||||||:||:|||||.|.:|:...::  .:.
Human  1214 LAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRV 1278

  Fly  1244 EKLRINLNSVTLANLLEYVHVSTGLTLDPERS--YEYDMRFQFLPREVYKEDYGVRPKHIIKYMH 1306
            :.|:..|..|.|..:|:.:.|.....::.:::  ..|.:||||||...|:::..:||:.|:::|.
Human  1279 KSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFME 1343

  Fly  1307 QTFFKQLIRAILKVSNASRTTKIVVIDDKKDADKEDDNDLDNGDEVGRSKAKANDD--------D 1363
            ..|||.|:.:|.|.:|.:...:.|      :..:....||||..|:|||:.:...|        |
Human  1344 TRFFKLLMESIKKKNNKASAFRNV------NTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVD 1402

  Fly  1364 SSDDNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDANDDDDEAED------------EDDEEKG 1416
            :..:..|.||:..|.|:::.:|.||:..::.|...:.|||:|..|:            |.|||.|
Human  1403 AEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVG 1467

  Fly  1417 QDGNDNDGD-------------------DKAVERLLS-----NDMVKAYTYDKENHLWCQVKLNL 1457
            . |.:.|..                   .:|:||.:.     :..:..|.||.|..|||||.:.|
Human  1468 L-GTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKL 1531

  Fly  1458 SVRYQKPDLTSIIRELAGKSVVHQVQHIKRAIIYKGTDD----DQLLKTDGINIGEMFQHNKILD 1518
            .:.....|::|::..||..:|::..:.|.|.::.:.|::    :.:|.|:|||:.|:|::.::||
Human  1532 PLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLD 1596

  Fly  1519 LNRLYSNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKG 1583
            |.|||||||||||.|||||||.:||.||:.:||.||||.||.|||||:||||.|:|.::||:|.|
Human  1597 LRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFG 1661

  Fly  1584 MEHSSSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRNGTGAFEL 1637
            :..:|||||||:||:|.||||.|...|..|||.|||:.|:||..||.|||.|||
Human  1662 IRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFEL 1715

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr1A	NP_523743.1	RNAP_I_RPA1_N	18..982	CDD:259844	463/1019 (45%)
		HMG-box_SF	1065..1119	CDD:438789	15/53 (28%)
		RNAP_I_Rpa1_C	1166..1638	CDD:132722	223/524 (43%)
POLR1A	NP_056240.2	RNAP_I_RPA1_N	16..1004	CDD:259844	463/1020 (45%)
		Clamp. /evidence=ECO:0000269|PubMed:34671025	110..201		22/109 (20%)
		Clamp. /evidence=ECO:0000269|PubMed:34671025	320..426		29/106 (27%)
		Rudder. /evidence=ECO:0000269|PubMed:34671025	403..416		3/12 (25%)
		Involved in RRN3 binding to Pol I complex. /evidence=ECO:0000269|PubMed:34887565	468..542		36/73 (49%)
		Funnel. /evidence=ECO:0000269|PubMed:34671025	805..883		28/77 (36%)
		RNA_pol_Rpb1_5	958..1670	CDD:398596	302/721 (42%)
		Bridging helix. /evidence=ECO:0000250|UniProtKB:G3MZY8, ECO:0000269|PubMed:34671025	960..1001		35/40 (88%)
		Mediates the interaction with TOP2A. /evidence=ECO:0000269|PubMed:36271492	1060..1155		30/95 (32%)
		Trigger loop. /evidence=ECO:0000250|UniProtKB:G3MZY8	1207..1248		38/40 (95%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1365..1498		32/139 (23%)