DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI1 and Polr2a

DIOPT Version :9

Sequence 1:	NP_523743.1	Gene:	RpI1 / 36617	FlyBaseID:	FBgn0019938	Length:	1642	Species:	Drosophila melanogaster
Sequence 2:	NP_001277997.1	Gene:	Polr2a / 20020	MGIID:	98086	Length:	1970	Species:	Mus musculus

Alignment Length:	1741	Identity:	498/1741 - (28%)
Similarity:	761/1741 - (43%)	Gaps:	405/1741 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 LEFAVFTDQEIRKLSVVKVITGITFDAL---GHAIPGGLYDIRMG---SYGRCMDPCGTCL-KLQ 73
            ::|.|.:..|::::||.:  .||.:...   |....|||.|.|.|   ..|||.    ||. .:.
Mouse    21 VQFGVLSPDELKRMSVTE--GGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQ----TCAGNMT 79

  Fly    74 DCPGHMGHIELGTPVYN-PFFIKFVQRLLCIFCLHCYKLQMKDHECEIIML--------QLRLID 129
            :||||.|||||..||:: .|.:|.::.|.|: |..|.||.:..:..:|..:        :.||
Mouse    80 ECPGHFGHIELAKPVFHVGFLVKTMKVLRCV-CFFCSKLLVDSNNPKIKDILAKSKGQPKKRL-- 141

  Fly   130 AGYIIEAQELELFKSEIVCQNTENL-----VAIKNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSC 189
                  ....:|.|.:.:|:..|.:     |....||                |..:.......|
Mouse   142 ------THVYDLCKGKNICEGGEEMDNKFGVEQPEGD----------------EDLTKEKGHGGC 184

  Fly   190 S-LRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFAD 253
            . .:..|..|.|: |..:.:|.|:..:    ..:.|:....:.:|.:|:..             :
Mouse   185 GRYQPRIRRSGLE-LYAEWKHVNEDSQ----EKKILLSPERVHEIFKRISD-------------E 231

  Fly   254 EC------RRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRARPLNM 312
            ||      .||.|      ||.:.:.|                         |||.|...||
Mouse   232 ECFVLGMEPRYAR------PEWMIVTV-------------------------LPVPPLSVRP--- 262

  Fly   313 VGDMLKGNP--QTDIYINIIENNHVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETAHEKLYTAWL 375
             ..:::|:.  |.|:       .|.|..::|.  ..|.:..|:..||...:..:.   ||
Mouse   263 -AVVMQGSARNQDDL-------THKLADIVKI--NNQLRRNEQNGAAAHVIAEDV---KL----- 309

  Fly   376 ALQMSVDVLLD---VNMSREM-KSG---EGLKQIIEKKSGLIRSHMMGKRVNYAARTVITPDPNI 433
             ||..|..::|   ..:.|.| |||   :.|||.::.|.|.:|.::|||||:::||||||||||:
Mouse   310 -LQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNL 373

  Fly   434 NVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRE 498
            ::|::|:|...|..:::...||.:|:..|:::|..|...:|||.||...||       |....|
Mouse   374 SIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNG-------DRIDLR- 430

  Fly   499 SLAKLLLSNPKD-----GIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHGEKTFRLHYSN 558
                 ....|.|     |.| |.||:.:||:::.||||:|||.|:|||:.|||.. .||||:.|.
Mouse   431 -----FHPKPSDLHLQTGYK-VERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPW-STFRLNLSV 488

  Fly   559 CKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGR 623
            ...|||||||||||.|.|||...|||...|..|....:.|:...|:.|::||.:.:..|.:.|..
Mouse   489 TTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDV 553

  Fly   624 FFNREDYQQLVFQGLSQLKKDIKLLPPTILKPAVLWSGKQILSTII---INIIPEGYERINLDSF 685
            |..|.:...|: ..||..  |.|:..|.||||..||:||||.|.||   ||.|     |.:
Mouse   554 FLERGEVMNLL-MFLSTW--DGKVPQPAILKPRPLWTGKQIFSLIIPGHINCI-----RTH---- 606

  Fly   686 AKIAGKNWNVSRPRPPICGTNPEGNDL--------SESQVQIRNGELLVGVLDKQQYGATTYGLI 742
                              .|:|:..|.        .:::|.:.||||::|:|.|:..|.:...|:
Mouse   607 ------------------STHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLV 653

  Fly   743 HCMYELYGGDVSTLLLTAFTKVFTFFLQLEGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVA 807
            |..|...|.|::.|..:....|...:|.:||.|:|:.|    .:||   .|..::.:|....|..
Mouse   654 HISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGD----SIAD---SKTYQDIQNTIKKAKQ 711

  Fly   808 AALELEDEPPHDELVEKMEAAYVKDSKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNL 872
            ..:|:.::..::||..      ...:..|...:.:...:|:...:...|:.  :..::::  ||.
Mouse   712 DVIEVIEKAHNNELEP------TPGNTLRQTFENQVNRILNDARDKTGSSA--QKSLSEY--NNF 766

  Fly   873 QLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGI 937
            :.||:||||||.:|..|:..::||..:||||.|.....::||.|...:..|:|.||::..::.|:
Mouse   767 KSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGL 831

  Fly   938 QPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDG- 1001
            .|.:||||.|.||||||||||||:.:||:||.|||.:|.:.|.||.|||:|.|.|||..|||||
Mouse   832 TPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896

  Fly  1002 ---------LDILK--SKFFNDKFCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWE 1055
                     |..||  :|.|..||..|:  .|..|:.|..|..|:||....|.:|.
Mouse   897 AGESVEFQNLATLKPSNKAFEKKFRFDY--TNERALRRTLQEDLVKDVLSNAHIQN--------- 950

  Fly  1056 KKKPAKLRAAFTHFSEELREEVEVKR---------------------------------PNEINS 1087
                 :|...|    |.:||:.||.|                                 |::::.
Mouse   951 -----ELEREF----ERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHP 1006

  Fly  1088 KTGRRRFDEGLLKLWKK---ADAEDKALYRKKYARCPDPTVAVYKQDLYYGSVSERT---RKLIT 1146
                .:..||:.:|.||   .:.:|           |....|.....|.:......|   |::..
Mouse  1007 ----IKVVEGVKELSKKLVIVNGDD-----------PLSRQAQENATLLFNIHLRSTLCSRRMAE 1056

  Fly  1147 DYAKRKPALKETIADIMRVKTIKSLAAPGEPVGLIAAQSIGEPSTQMTLNTFHFAGRGEMNVTLG 1211
            ::.....|....:.:| ..|..:::|.|||.||.:||||:|||:|||||||||:||....|||||
Mouse  1057 EFRLSGEAFDWLLGEI-ESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLG 1120

  Fly  1212 IPRLREILMLASSNIKTPSMDIPIKPGQQHQAEKLR---INLNSVTLANLLEYVHVSTGLTLDPE 1273
            :|||:|::.: |...||||:.:.:.......||:.:   ..|...||..    |..:|.:..||.
Mouse  1121 VPRLKELINI-SKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRK----VTANTAIYYDPN 1180

  Fly  1274 RSYEYDMRFQFLPRE-VYKEDYGVRPKHIIKYMHQTFFKQLIRAILKVSNASRTTKIVVIDDKKD 1337
                        |:. |..||    .:.:..|.....|        .|:..|.....|.:|.|..
Mouse  1181 ------------PQSTVVAED----QEWVNVYYEMPDF--------DVARISPWLLRVELDRKHM 1221

  Fly  1338 ADKEDDNDLDNGDEVGRSKAKANDDDSSDDNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDAND 1402
            .|::    |.......:..|...||.:...|||:   ..||               |..:...|.
Mouse  1222 TDRK----LTMEQIAEKINAGFGDDLNCIFNDDN---AEKL---------------VLRIRIMNS 1264

  Fly  1403 DDDEAEDEDDEEKGQDGNDNDGDDKAVERLLSNDMVKAYTYDKENHLWCQVKLNLSVRYQKPDLT 1467
            |:::.:   :||:..|..|:|...:.:|..:..||.                           |.
Mouse  1265 DENKMQ---EEEEVVDKMDDDVFLRCIESNMLTDMT---------------------------LQ 1299

  Fly  1468 SIIRELAGKSVVH--QVQHIKRAIIYKGTDDDQ-------LLKTDGINIGEMFQHNKILDLNRLY 1523
            .|  |...|..:|  |..:.|:.||   |:|.:       :|:|||:::..:.. .|.:|..|..
Mouse  1300 GI--EQISKVYMHLPQTDNKKKIII---TEDGEFKALQEWILETDGVSLMRVLS-EKDVDPVRTT 1358

  Fly  1524 SNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGM-EHS 1587
            ||||..|....||||..:.:.:|:.:|....|..|:.|||:|:.|.||..|....::|.|: ...
Mouse  1359 SNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQD 1423

  Fly  1588 SSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRNGTGAFELL 1638
            :.||.:.|||.::..|..||..|.:|.:...|..:|:|.....|||.|:||
Mouse  1424 TGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLL 1474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI1	NP_523743.1	RNA_pol_rpoA1	14..1009	CDD:274106	322/1057 (30%)
		RNAP_I_RPA1_N	18..982	CDD:259844	304/1016 (30%)
		RNA_pol_Rpb1_5	936..1593	CDD:282807	205/721 (28%)
		HMG-box	1057..1119	CDD:294061	15/97 (15%)
		RNAP_I_Rpa1_C	1166..1638	CDD:132722	138/485 (28%)
Polr2a	NP_001277997.1	RNAP_II_RPB1_N	19..876	CDD:259848	304/1018 (30%)
		Bridging helix	833..845		7/11 (64%)
		RNA_pol_Rpb1_6	896..1079	CDD:309914	40/218 (18%)
		RNAP_II_Rpb1_C	1058..1476	CDD:132720	142/505 (28%)
		CTD	1491..1610	CDD:215026
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1546..1970
		C-terminal domain (CTD), 52 X 7 AA approximate tandem repeats of Y-[ST]-P-[STQ]-[ST]-P-[SRNTEVKGN]	1593..1960
		Herpes_BLLF1	<1857..>1961	CDD:330317
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.820