DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpI1 and Polr1a

DIOPT Version :9

Sequence 1:	NP_523743.1	Gene:	RpI1 / 36617	FlyBaseID:	FBgn0019938	Length:	1642	Species:	Drosophila melanogaster
Sequence 2:	NP_033114.3	Gene:	Polr1a / 20019	MGIID:	1096397	Length:	1717	Species:	Mus musculus

Alignment Length:	1730	Identity:	753/1730 - (43%)
Similarity:	1056/1730 - (61%)	Gaps:	140/1730 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 LEFAVFTDQEIRKLSVVKVITGITFDALGHAIPGGLYDIRMGSYGRCMDPCGTCLK-LQDCPGHM 79
            :.|.:::.:|::||||..:......|.||:....||||:.:|. ....:.|.||:: ..:|.||:
Mouse    15 ISFGMYSAEELKKLSVKSITNPRYVDYLGNPSANGLYDLALGP-ADSKEVCATCVQDFNNCSGHL 78

  Fly    80 GHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAGYIIEAQELELFKS 144
            |||:|...|||||....:..||...||.|:.|........:::.|||:::.|.:....|||...|
Mouse    79 GHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELERILS 143

  Fly   145 EIVCQNTENLVAIKNGDMVHPHIAAMYKLLEKNEKNSSNS-TKTSCSLRTAI------THSALQR 202
            ..: :.|.:..|.:..:.:..:.:   |:|:.|...|..: .|..|..|:.:      ||.|
Mouse   144 RFL-EETGDPSAFEIQEELEEYTS---KILQNNLLGSQGTHVKNVCESRSKLVAQFWKTHMA--- 201

  Fly   203 LGKKCRHCNKSMRFVRYMHRR---LVFYVTLADIKERVGTGAETG------------GQNKVIFA 252
             .|:|.||......||..|..   :.:..|:....::.||....|            |:...:..
Mouse   202 -AKQCPHCKTGRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTP 265

  Fly   253 DECRRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVD--LFFMDTLPVTPPRARPLNMVGD 315
            ...:.:|..|:.|....|..|..  ||.:.    |..|..:  :||:|.:.|.|.|.||:|.:||
Mouse   266 SSAQEHLFAIWKNEGFFLNYLFS--GLDDI----GPESSFNPSMFFLDFIVVPPSRYRPVNRLGD 324

  Fly   316 MLKGNPQTDIYINIIENNHVLNVVLKYMKGGQEKLTEEA----------------KAAYQTLKGE 364
            .:..|.|| :.:..:..:.||...|..:...::||..|.                ::....|.|.
Mouse   325 QMFTNGQT-VNLQAVMKDAVLIRKLLALMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGP 388

  Fly   365 TAHEKLYTAWLALQMSVDVLLDVNMSREM-KSGEGLKQIIEKKSGLIRSHMMGKRVNYAARTVIT 428
            :..:|||..|:.||..|:::.|..|.:.| :...|::||:|||.||.|.|||||||:||||:||.
Mouse   389 SLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVIC 453

  Fly   429 PDPNINVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADN 493
            ||..||.:|||||.:||.||:||.|||.|||.|||:.|:|||:|||||:.:.:::|..|.:.:.:
Mouse   454 PDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSSVD 518

  Fly   494 ASKRESLAKLLLS----NPK-DGIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHGEKTFR 553
            |::||::||.||:    .|| .|.|:|.|||.|||:|||||||:||:|||..|:||||..||..|
Mouse   519 AAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLR 583

  Fly   554 LHYSNCKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKL 618
            |||:|||||||||||||||||:||||:.|||||.|......|||||||.||.||||||::||..:
Mouse   584 LHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANM 648

  Fly   619 SIRGRFFNREDYQQLVFQGLSQLKKDIKLLPPTILKPAVLWSGKQILSTIIINIIPEGYERINLD 683
            :|||.||.||.|.:||::||:.....:||.||.||||..||:|||::||::||||||.|..:||.
Mouse   649 TIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLS 713

  Fly   684 SFAKIAGKNWNVSRPRPPICGTNPEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGLIHCMYEL 748
            ..|||..|.|...:|| ||...:|:  .:.||||.||.||||.|||||..||::.|||:||.||:
Mouse   714 GKAKIGSKAWVKEKPR-PIPDFDPD--SMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEI 775

  Fly   749 YGGDVSTLLLTAFTKVFTFFLQL-EGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVAAALEL 812
            |||:.|..:||...::||.:||| .||||||:||||...||..|::||.|....|..||.|||.|
Mouse   776 YGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSL 840

  Fly   813 EDEPPHDELVEKMEAAYV-KDSKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNLQLMV 876
            .:....||:..|.:.|:: ||.:...::|.|:|..::.|:|:||..|:|.||..:||.||||:||
Mouse   841 PETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMV 905

  Fly   877 LSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGIQPQD 941
            .||||||.|||||||||||||||||:|||||.||||||.|..:|.:|::|||:.|||:|||:|.:
Mouse   906 QSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPE 970

  Fly   942 FFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDGLDILK 1006
            ||||||||||||:|||||||||||||||:|||||||.:.|||||||||.||||||||||||||.|
Mouse   971 FFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPK 1035

  Fly  1007 SKFFNDK-FCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWEKKKPAKL--RAAFTH 1068
            ::|...| |  .||..|...|::...|..:.......||..|.|.|:.|..|....|  :.||..
Mouse  1036 TQFLQPKQF--PFLAGNYEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLS 1098

  Fly  1069 FSEELREEVEVKRPNEINSK----TGRRRFDEGLLKLWKKADAEDKALYRKKYARCPDPTVAVYK 1129
            ||::::..|:.     :|.|    .||....:.:|::|...|.|.:..|:|:.|.||||:::|::
Mouse  1099 FSQKIQAAVKA-----LNLKGSIQNGRSPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWR 1158

  Fly  1130 QDLYYGSVSERTRKLITDY-----AKRKPALKETIADIMRVKTI------KSLAAPGEPVGLIAA 1183
            .|:|:.||||...|.|.|:     |:.:.:.|::...:.|::|:      :||..|||.|||:||
Mouse  1159 PDIYFASVSETFEKKIDDFSQEWAAQAERSYKKSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAA 1223

  Fly  1184 QSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPGQQ--HQAEKL 1246
            |||||||||||||||||||||||||||||||||||||:||:|||||.|.:|:...::  .:.:.|
Mouse  1224 QSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSL 1288

  Fly  1247 RINLNSVTLANLLEYVHVSTGLTLDPERS--YEYDMRFQFLPREVYKEDYGVRPKHIIKYMHQTF 1309
            :..|..|.|..:|:.|.:.....:..:|:  ..|::||||||...|:::..:||:.|:.:|...|
Mouse  1289 KKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRF 1353

  Fly  1310 FKQLIRAILKVSNASRTTKIVVIDDKKDADKEDDNDLDNGDEVGRSKAKANDDDSSDDN------ 1368
            ||.|:.||.|..|.:...:.|   :.:.|.::|.||.::.   |||:.:...|:..:.|
Mouse  1354 FKLLMEAIKKKKNKASAFRNV---NSRRATQKDLNDTEDS---GRSQREEERDEEEEGNIVDAEA 1412

  Fly  1369 --DDDDATGVKLKQRKTDEKDYDDPDDVEE----------------LHDANDDDDE----AEDED 1411
              .|.||:..|.|:::.:|.||:..::.||                :|..::.|:|    .|:|:
Mouse  1413 EEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEHLGLEEEE 1477

  Fly  1412 DEEKGQDGNDNDGDDKAVERLL-----SNDMVKAYTYDKENHLWCQVKLNLSVRYQKPDLTSIIR 1471
            ..:|....:......:|::|.:     |...::.|.||.|..|||||.:.|.:.....|::|::.
Mouse  1478 SSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVV 1542

  Fly  1472 ELAGKSVVHQVQHIKRAIIYKGT----DDDQLLKTDGINIGEMFQHNKILDLNRLYSNDIHAIAR 1532
            .||.|::|:..:.|.|.::.:.|    :.:.:|.|:|||:.|:|::::||||.|||||||||:|.
Mouse  1543 SLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMAN 1607

  Fly  1533 TYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGMEHSSSPLQQMSFE 1597
            |||||||.:||.||:.:||.||||.||.|||||:||||.|:|.::||:|.|::.||||||||:||
Mouse  1608 TYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFE 1672

  Fly  1598 SSLQFLKSAAGFGRADELSSPSSRLMVGLPVRNGTGAFEL 1637
            :|.||||.|...|..|||.|||:.|:||..|:.|||.|||
Mouse  1673 TSFQFLKQATMMGSHDELKSPSACLVVGKVVKGGTGLFEL 1712

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpI1	NP_523743.1	RNA_pol_rpoA1	14..1009	CDD:274106	484/1041 (46%)
		RNAP_I_RPA1_N	18..982	CDD:259844	460/1012 (45%)
		RNA_pol_Rpb1_5	936..1593	CDD:282807	302/715 (42%)
		HMG-box	1057..1119	CDD:294061	17/67 (25%)
		RNAP_I_Rpa1_C	1166..1638	CDD:132722	220/519 (42%)
Polr1a	NP_033114.3	RNA_pol_rpoA1	10..1038	CDD:274106	484/1041 (46%)
		RNAP_I_RPA1_N	16..1011	CDD:259844	460/1013 (45%)
		RNA_pol_Rpb1_5	965..1668	CDD:282807	302/715 (42%)
		Bridging helix. /evidence=ECO:0000250	968..980		9/11 (82%)
		RNAP_I_Rpa1_C	1206..1713	CDD:132722	219/513 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1372..1493		26/126 (21%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167846160
Domainoid	1	1.000	516	1.000	Domainoid score	I313
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H7033
Inparanoid	1	1.050	1288	1.000	Inparanoid score	I163
Isobase	1	0.950	-	0	Normalized mean entropy	S1549
OMA	1	1.010	-	-		QHG54249
OrthoDB	1	1.010	-	-		D57214at33208
OrthoFinder	1	1.000	-	-		FOG0005035
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101773
Panther	1	1.100	-	-	LDO	PTHR19376
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1172
SonicParanoid	1	1.000	-	-		X3561
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.750