Protein Alignment Shrm and shroom4

DIOPT Version :10

• Sequence 1: NP_001097307.1
• Gene: Shrm / 36592
• FlyBaseID: FBgn0085408
• Length: 1576
• Species: Drosophila melanogaster
• Sequence 2: XP_005172390.1
• Gene: shroom4 / 559035
• ZFINID: ZDB-GENE-041111-246
• Length: 1634
• Species: Danio rerio
• Alignment Length: 1821
• Identity: 353/1821 - (19%)
• Similarity: 591/1821 - (32%)
• Gaps: 598/1821 - (32%)

  Fly    10 GNGSEMG------------ESTKSLAKME----PEN----NNKISVVSVSKLLLKDSNGANSRSS 54
            |:.||:.            :.:.|:..||    |.|    ::::|.:.......|..:..:|.|:
Zfish   142 GDNSELSMQWGHISRHYSTDRSSSIGSMESLENPPNQGYYDSQLSPIDPVIFNNKRDSAYSSFSA 206

  Fly    55 NSNASFSSASVAGSVQDDLPHHNSSSSQLGQQHGSSLDQCGLTQAGLEE----YNNRSSSYYDQT 115
            :||.|..:..|.       |...:|...|.|..|||   |.....|...    :.|:........
Zfish   207 SSNTSDYTVPVK-------PEETNSMDSLLQGLGSS---CRYPDRGQHSAPTGHGNQLEEQEHSK 261

  Fly   116 AFHHQKQPSYAQSEGYHSYVSSSDSTSATPFLDKLRQESDLLSR-------------------QS 161
            ....:.:|....|    ||  |.:..:..|....:|:||...:|                   ..
Zfish   262 VLSGRSEPKVRPS----SY--SCEEENCAPPQPPMRKESFRATRGQATDKRCVSAPVGIPNVSSC 320

  Fly   162 HHWSENDLSSVCSNSVAPSPI-------------PLLARQSHSHSHSHAHSHSNSHGHSHGHAHS 213
            ....::.:..|.:..|..:.|             |.....|.|.....|..|...|...:..:.:
Zfish   321 TDEDQSQIQDVLTGRVYLNGIQDSEPERKGCSIQPYYTLNSDSGGDGKATDHEKEHLEDYSKSVA 385

  Fly   214 ASSSSSSNNNSN-------------GSATNNNN---------------NNSSESTSSTETLKWLG 250
            |:||..|..|.:             .||.|.::               :......|::.|.:|..
Zfish   386 AASSPPSQRNRDENFDTKLRPEHDLQSAMNRHSAPEKLLAAQLCMMDVSADKSDHSASPTCQWSQ 450

  Fly   251 SMSDISEASHATGYSAISESVSSSQRIVHSSRVPTPKRHHSESVLYLHNNEEQG-----DSSPTA 310
            |...:|::|..|.......|..|:...|.:|.|..| |...||:   ......|     ..||..
Zfish   451 SPLYLSDSSQNTEQGKWGTSRCSTPGSVATSEVEDP-RLEEESI---DGQNPWGHPISLSGSPIG 511

  Fly   311 SNSSQMMISEEANGEE-------SPPSVQPLRIQHRHSPS--YPPVHTSMVLHHFQQQQQQQQDY 366
            :..||   :.|.||.|       .|.  ||.:.|.|.|.|  ......:.:.:..|:::.|.|..
Zfish   512 NGFSQ---NSENNGGEVMLDDGLGPK--QPQKRQFRCSKSRRRSERFATNLRNEIQRKKAQLQKS 571

  Fly   367 QHPSRHHTNQSTLSTQSSLLELASPTE----KPRSLMGQSHSMGDLQQKNPHQNPMLGRSAGQQH 427
            ::|....|.:..:...::  |:.:|.|    ||:.|...:.:...:|...|....:...:..:::
Zfish   572 RNPCGEETVEEEVGDFNT--EVVAPPEYRQHKPQILPKPATNTVPIQISKPATQKVFDATQTEEY 634

  Fly   428 KSSISVTISSSEAVVTIAPQ-------------PPAGKP--------SKLQLSLGKSEA------ 465
            .|:..|...:|: ..|..||             .|||||        .||:|....||:
Zfish   635 SSARVVCFQTSQ-TQTQVPQHVRQVCVHVVEEVAPAGKPRRWRWTPERKLELENESSESKRNETI 698

  Fly   466 --------------LSCSTPNMGEQSPTNSIDSYRSNHRLFPVSTYTEPVHSNTSQYVQHPKPQF 516
                          .|.|:..:| :|....|..:....|.|          ..||:.:.......
Zfish   699 GQMWSGKMESTRSRTSSSSGRLG-RSDDCDILPFADRRRFF----------EETSRKLSQSVTNL 752

  Fly   517 SSGLHKSAKLPVITPAGATVQPTWHSVAERINDFERSQLGEPPKFAYLEPTKTHRLSNP----AL 577
            |:...:|.:|...........|..|.:|        :.||. .:|:|.:....:.|.|.    :.
Zfish   753 SNLTSRSQRLDKPGRMNHPSSPEPHEIA--------AHLGR-RRFSYQDVPYINSLDNGRQFISN 808

  Fly   578 KALQKNAVQSYVERQQQQQKE-----EQQLLRPHSQSYQACHVERKSLPNNLSPIMVGLPTGSNS 637
            :..|:...|..:||:|::::|     ||:.||...:..:....||::....|
Zfish   809 QQDQEKTRQRLIEREQEREREQERLKEQEKLREQEREKERLRKERENEQQRL------------- 860

  Fly   638 ASTRDCSSPTPPPPPRRSGSLLPNLLRRSSSASDYAEFRELHQAQGQVKGPSIRNISNAEKISFN 702
                                            .|:.|.:.|.|.:.:.:  |:|...::|
Zfish   861 --------------------------------KDWEERQRLLQREQEWE--SVRESVHSE----- 886

  Fly   703 DCGMPPPPPPPRGRLAVPTRRTSSATEYAPMRDKLLLQQAAALAHQQHHPQQHRHAQPPHVPPER 767
                               ..|||.|                :.|...|....:  ||
Zfish   887 -------------------HNTSSDT----------------VPHSLSHDVYRK--QP------- 907

  Fly   768 PPKHPNLRVPSPELPPPPQSELDISYTFDEPLPPPPPPEVLQPRPP--PSPNRRNCFAGASTRRT 830
                     |||::|    |.|.:|    |||.......|.:|...  |...:.|.:.|
Zfish   908 ---------PSPQIP----SSLQMS----EPLYSSTTTNVQKPCSAFHPVTTQHNRYDG------ 949

  Fly   831 TYEAPPPTAIVAAKVPPLVPKKPTSLQHKHLANGGGGSRKRPHHATPQPILENVASPVAPPPPLL 895
             |:..||                                ..|.:.||
Zfish   950 -YQVNPP--------------------------------YNPRNYTP------------------ 963

  Fly   896 PRARSTAHDNVIASNLESNQQKRSNSKASYLPRQ-SLEKLNNTDPDHGIYKLTLTSNEDLVAHTK 959
                                     .:|.|:..| ...|:|.        ..:||..:
Zfish   964 -------------------------DEAQYVREQEKTRKINR--------NFSLTERD------- 988

  Fly   960 PSYGVTGKLPNNLPDVLPLGVKLHQQPKLQPGSPNGDANVT--LRYGSNNNLTGNSPTVAPPPYY 1022
                    .|.:..|.           |...|     |||.  :|.|.|:..:|.:.
Zfish   989 --------YPRSRRDF-----------KTVDG-----ANVPGFVRTGGNSITSGRNE-------- 1021

  Fly  1023 GGGQRYSTPVLGQGYGKSSKPVTPQQY-TRSQSYDVKHTSAVTMPTMSQSHVDLKQAAHDLETTL 1086
               :...||:..:...:::..|..|.| ..|.|..|....:.|:..:.::.|           ::
Zfish  1022 ---EHLFTPLRNRAMSENNLCVETQHYQNHSSSSGVSRMRSSTLSDLDENTV-----------SV 1072

  Fly  1087 EEVLPTATPTPTPTPTPTPPRLSP-----ASSHSDCSLSTSSLECTINPIATPIPKPEAHIFRAE 1146
            .|:.....|   |.|.|.||:...     ||.||..| |||...   :|.|:| |:|:..
Zfish  1073 GEIKKKKGP---PPPRPPPPKWDQFHKRRASHHSLFS-STSHYS---SPTASP-PRPQPQ----- 1124

  Fly  1147 VISTTLNTNPLTTPPKPAMNRQESL----RENIEKITQLQSVLMSAHLCDASLLGGYTTPLIT-- 1205
                |..:.|   |....|:||.|.    |:..|          |.|.|........::|..|
Zfish  1125 ----TCTSRP---PSVSEMSRQRSYSLPPRDMSE----------SLHCCRQEYSVAPSSPAFTRR 1172

  Fly  1206 --SPTA-------------SFANEPLMTPPLPPSPPP---------PLEPEEEEEQEENDVH--- 1243
              .|.|             ....||....|:.|:..|         |..|:...|.:.:..|
Zfish  1173 AFKPVALPPREMDYNTRREVHQPEPCTRIPIHPAAAPEEPRATHLKPTFPDHGVEWDRSSPHYPT 1237

  Fly  1244 ---DKQPEIE--------------------ELQLMQ--------RSELVLMVNPKPSTTDMACQT 1277
               .:.||:.                    :||..|        :::::....|:.....:....
Zfish  1238 HGTSRTPEVPSNSFSAGPLCPESYFSMNNFQLQPQQAGFPITAHKTQIIPSQGPEDGDLPLETDI 1302

  Fly  1278 DELEDRDTDLEAAREEHQTRTTLQ--PRQRQPIELDYEQMSRELVKLL-----PPGDKIADILTP 1335
            ||:.:.:   :|.|:|...|..:|  .|....:|.|.:....|.....     |.|..:..||..
Zfish  1303 DEICENE---QAERKETVDRMEMQGFARPVMVLETDIDHTPEEAAPSAISIRGPRGSLVDSILED 1364

  Fly  1336 KICKPTSQYVSNLYNPDVPLRLA------KRDVGTSTLMRMKSITSSA---------EIRVVSVE 1385
            :......:.:..|:...|...::      ...:...||.|...:.::|         :|...:.:
Zfish  1365 EYGVSRKELLGELFPHSVNAEMSGDGWRGGYPISGGTLERPSRLNTTAPQVSRSTCYDISADNPQ 1429

  Fly  1386 L-----QLAEPSEEPTNLIKQKMDELIKHLNQKIVSLKREQQTISEECSANDRLGQDLFAKLAEK 1445
            |     :::|..||...|..:|  :|::.|.:|:..|:..|:.:.|:..||.:||.::.:.:...
Zfish  1430 LLAKLREISERKEEDEELNYKK--QLMESLRKKLTVLREAQRGLQEDIRANAQLGDEVESLVLAI 1492

  Fly  1446 VRPSEASKFRTHVDAVGNITSLLLSLSERLAQTESSL-----ETRQQERGALESKRDLLYEQMEE 1505
            .:|:|..|:|..:..:..:||||||||.||.:.||:|     ||..|||..|..|:..|..||.|
Zfish  1493 CKPNEVDKYRMFIGDLDKVTSLLLSLSGRLIRVESALDCVDPETGHQERLQLLEKKKQLLVQMGE 1557

  Fly  1506 AQRLKSDIERRGVSIAGLLAKNLSADMCADYDYFINMKAKLIADARDLAVRIKGSEEQLSSLSDA 1570
            ||.||..::||..::..:|...|:.:...||.:|:.|||.|:.:.|.|..:|:..||||..|.::
Zfish  1558 AQELKEHVDRREQAVCRVLGCCLTPEQMRDYGHFVKMKAALLVEQRQLDDKIRLGEEQLRGLRES 1622

  Fly  1571 L 1571
            |
Zfish  1623 L 1623

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Shrm	NP_001097307.1	ASD2	1318..1571	CDD:462561	79/282 (28%)
shroom4	XP_005172390.1	PDZ_shroom2_3_4-like	11..92	CDD:467232
		CCDC66	<775..>874	CDD:434558	25/152 (16%)
		ASD2	1372..1623	CDD:462561	74/252 (29%)