Protein Alignment Ih and hcn4

DIOPT Version :10

• Sequence 1: NP_001033948.1
• Gene: Ih / 36589
• FlyBaseID: FBgn0263397
• Length: 1327
• Species: Drosophila melanogaster
• Sequence 2: XP_002944030.2
• Gene: hcn4 / 100498501
• XenbaseID: XB-GENE-940130
• Length: 2061
• Species: Xenopus tropicalis
• Alignment Length: 1348
• Identity: 470/1348 - (34%)
• Similarity: 642/1348 - (47%)
• Gaps: 341/1348 - (25%)

  Fly    32 PDNQQQQQQRH------SHSHQHL--------VRQSLVSLSNGQPAASAAPDSVSLLRAGDDQQQ 82
            |.:...|...|      |||...:        :.||.|.:|....|......|..|.....||.
 Frog   681 PSHSPAQVPSHSPAQVPSHSPAQVSSQFSTQGLNQSPVQISRKASAQGLNQHSAPLSSLPSDQM- 744

  Fly    83 HPQQPHLQQQQQQQQHLQQQQQRHRSSSSRLSTSGISKQNSSDSRSGLRILDS------------ 135
             |..||      ...|...|...|  ..|:....|.|..:....::.:::|:.
 Frog   745 -PSWPH------ASVHGLSQPSPH--GPSQFCAQGPSPSSEGSIQAPVQLLNQPPVQGLSQCPSQ 800

  Fly   136 --SHSPVSCGTQSVSSTGGQSALYDACHEYSRSLSAAAAEGAASLLKSHYSDQQLAQTEPDPEPD 198
              ||||....:.|.|.....|......|..::..|.:.|:     :.||     .|...|.|.|.
 Frog   801 VPSHSPAQVASHSPSQVPSHSPAQVPSHSPAQVPSHSPAQ-----VPSH-----CALEGPKPSPV 855

  Fly   199 PERDRDRDRDRDRDRERERRHLTNLNLNLSSEYDYSGSDKQQLVNETYIFKCIANSPSFLRTNKI 263
            |                                   |:::.       |:..:..||:....::.
 Frog   856 P-----------------------------------GANQP-------IYPSVIKSPTQAPNHQP 878

  Fly   264 ----KEQSKKLRNLSLKTRTAKKKGQIISKSNAVSDNSLHPGDKYLNLYLVEKKHSLQPQVASTS 324
                ..||..|.::       :....:.|||:|::.:               :.::|.|   |.|
 Frog   879 NTHGNSQSPALESI-------QSPALVPSKSSALASS---------------QSNALVP---SQS 918

  Fly   325 SSINTTHPQASSAPASSSSTCTKAPQLPALSA-----VAARQQQLLLNGSLKGKGQSQSQGQSRQ 384
            |::   .|..|||.||..|......|.||||:     :...|...|:.......|.|||...:..
 Frog   919 SAL---VPSQSSALASRKSPALVPSQSPALSSNQSLVLVPSQSPALVPTQYSALGSSQSPALAPS 980

  Fly   385 TLPGHRASVRSESGSGSSHTIPATGKSPPVPHSLAAKISSSASGSKNCNLLSASSNSCHKLNAHA 449
            ..|       :.:.|.|...:|:  :||.:..|.:..::.|.|.:..|:...|.:.........:
 Frog   981 QSP-------TVACSQSPALVPS--QSPALVPSQSPALAPSQSPTVACSQSPALAPCQSPSVVPS 1036

  Fly   450 QGSGAGSGSGSGSGSGPPGHSHYAAAS---------PKSSVSSNGHLNKYCLTDLTRRKAEFNRQ 505
            |.|...|   |.|.:..|..|...|.|         |.:.:||..             .|:...|
 Frog  1037 QSSALAS---SQSPALAPSQSPALAPSQSPALVPTQPAAVISSES-------------PAQGPSQ 1085

  Fly   506 LSAPTDYTHHSSSNGSQQEGSSEANEG----------HEPVGESTI---TVASAGVSYPHPYSYP 557
              :|...:.|..:.||.|..:.|.::.          |.| .:..:   |.|:|..|.|
 Frog  1086 --SPFQGSCHVPTQGSIQSPAQEPSQSPAKVPSQCPLHVP-SQCPVRVPTQATAQDSCP------ 1141

  Fly   558 YHYAHHASSATAPANLKASLQLHSFGSHHPCPYPARPTSTSCTNSFNRRHIRRHKGKLGDRLLSG 622
                   .:|.||:...|.:..||         ||:.:|.|.....:
 Frog  1142 -------LTAQAPSQSPAQISSHS---------PAQISSQSPAQELS------------------ 1172

  Fly   623 DSEESVRCSYCSVLNVNDNDLRISFENTCTDSLVTAFDDEALLICDQGTEMVHFDDVSLYGTPKE 687
                                           ||:...         :|:|.....|
 Frog  1173 -------------------------------SLIPKL---------EGSEQTEVGD--------- 1188

  Fly   688 EPMPNIPIVSEKVSANFLKSQLQSWFQPTDNRLAMKLFGSRKALVKERIRQKTSGHWVIHPCSSF 752
                         ...|::.|..:..||..|:.::::|||.||:..|:.|.|::|.|:|||.|.|
 Frog  1189 -------------PGGFIQRQFGALLQPAVNKFSLRMFGSHKAVEIEQQRVKSAGSWIIHPYSDF 1240

  Fly   753 RFYWDLCMLLLLVANLIILPVAISFFNDDLSTRWIAFNCLSDTIFLIDIVVNFRTGIMQQDNAEQ 817
            ||||||.||||:|||||||||.|:||.|:.:..||.||.||||.||.|:|:||||||:.:||.|
 Frog  1241 RFYWDLIMLLLMVANLIILPVGITFFKDENTPPWIVFNVLSDTFFLADLVLNFRTGIVVEDNTE- 1304

  Fly   818 VILDPKLIAKHYLRTWFFLDLISSIPLDYIFLIFNQIMKLQDFSDSFQILHAGRALRILRLAKLL 882
            :||||..|...||::||.:|.:||||:|||||    |:.|:...|: ::....|||||:|..|:|
 Frog  1305 IILDPHTIKMKYLKSWFLVDFVSSIPVDYIFL----IVDLETNVDT-EVYKTARALRIVRFTKIL 1364

  Fly   883 SLVRLLRLSRLVRYVSQWEEVYILQNLQKKSADRRGRMNRKDKDGLTKSNLILKFLNMASVFMRI 947
            ||:||||||||:||:.||||::.:.                              .::||..:||
 Frog  1365 SLLRLLRLSRLIRYIHQWEEIFHMT------------------------------YDLASAVVRI 1399

  Fly   948 FNLICMMLLIGHWSGCLQFLVPMLQGFPSNSWVSINELQESYWLEQYSWALFKAMSHMLCIGYGR 1012
            ||||.||||:.||.|||||||||||.||.:.|||||.:....|.:|||.|:|||||||||||||:
 Frog  1400 FNLIGMMLLLCHWDGCLQFLVPMLQDFPEDCWVSINHMANESWGKQYSHAIFKAMSHMLCIGYGQ 1464

  Fly  1013 FPPQSLTDMWLTMLSMISGATCYALFLGHATNLIQSLDSSRRQYREKVKQVEEYMAYRKLPRDMR 1077
            ..|:.:||:||||||||.||||||:|:||||.||||||||||||:||.||||:||::.|||.|.|
 Frog  1465 QAPEGMTDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTR 1529

  Fly  1078 QRITEYFEHRYQGKFFDEELILGELSEKLREDVINYNCRSLVASVPFFANADSNFVSDVVTKLKY 1142
            |||.||:|||||||.||||.|||||||.|:|:::|:|||:|||::|.|||||.|||:.::|||::
 Frog  1530 QRIHEYYEHRYQGKMFDEENILGELSEPLKEEIVNFNCRNLVANMPLFANADPNFVTAMLTKLRF 1594

  Fly  1143 EVFQPGDIIIKEGTIGTKMYFIQEGVVDIVMANGEVATSLSDGSYFGEICLLTNARRVASVRAET 1207
            |||||||.||:|||:|.||||||.|||.| :..|...|.||||||||||||||..||.|||||:|
 Frog  1595 EVFQPGDYIIREGTVGKKMYFIQHGVVSI-LTRGSKETKLSDGSYFGEICLLTRGRRTASVRADT 1658

  Fly  1208 YCNLFSLSVDHFNCVLDQYPLMRKTMETVAAERLNKIG-KNPNIMHQKDEQLSNPESNTI----- 1266
            ||.|:|||||:||.||::||:||:..||||.:||::|| ||..::.::.|..|...:|.|
 Frog  1659 YCRLYSLSVDNFNEVLEEYPMMRRAFETVAMDRLDRIGKKNSILLRKRAENSSGSVNNEIIQQIV 1723

  Fly  1267 -----TA----VVNALAAEADDCKDDDMDLKENLLHGSESSIAEPVQT 1305
                 ||    .:..|||..|..|.   .:.|.|:|.       |:||
 Frog  1724 KHDRDTAHNIQDIQCLAATRDPNKP---FIWEPLVHA-------PLQT 1761

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ih	NP_001033948.1	Ion_trans_N	709..751	CDD:400630	16/41 (39%)
		PLN03192	741..>1214	CDD:215625	288/472 (61%)
hcn4	XP_002944030.2	PHA03307	16..>421	CDD:223039
		PHA03247	<458..961	CDD:223021	71/369 (19%)
		PHA03247	<740..1203	CDD:223021	118/682 (17%)
		Ion_trans_N	1197..1239	CDD:400630	16/41 (39%)
		PLN03192	1232..>1678	CDD:215625	296/482 (61%)
		CAP_ED	1576..1684	CDD:237999	74/108 (69%)