DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Hsc70-5 and dnaK

DIOPT Version :9

Sequence 1:	NP_001286414.1	Gene:	Hsc70-5 / 36583	FlyBaseID:	FBgn0001220	Length:	686	Species:	Drosophila melanogaster
Sequence 2:	NP_414555.1	Gene:	dnaK / 944750	ECOCYCID:	EG10241	Length:	638	Species:	Escherichia coli

Alignment Length:	638	Identity:	386/638 - (60%)
Similarity:	490/638 - (76%)	Gaps:	11/638 - (1%)

- Green bases have known domain annotations that are detailed below.

  Fly    52 GAVIGIDLGTTNSCLAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTF 116
            |.:|||||||||||:|:|:|...:|:|||||.|||||.:|:|:|||.|||.|||||||||..||.
E. coli     2 GKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTL 66

  Fly   117 YATKRLIGRRFDDPEVKKDITNLSYKVVKASNGDAWVSSTDGKVYSPSQIGAFILMKMKETAEAY 181
            :|.||||||||.|.||::|::.:.:|::.|.|||||| ...|:..:|.||.|.:|.|||:|||.|
E. coli    67 FAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWV-EVKGQKMAPPQISAEVLKKMKKTAEDY 130

  Fly   182 LNTPVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVINEPTAAALAYGMDK-TEDKIIAVYDL 245
            |..||..||:||||||||:|||||||||:||||.|.|:||||||||||||:|| |.::.||||||
E. coli   131 LGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDL 195

  Fly   246 GGGTFDISILEIQ----KGVFEVKSTNGDTLLGGEDFDNHIVNFLVAEFKKDSGIDIRKDNIAMQ 306
            |||||||||:||.    :..|||.:|||||.|||||||:.::|:||.|||||.|||:|.|.:|||
E. coli   196 GGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQ 260

  Fly   307 RLKEAAEKAKCELSSSQQTDINLPYLTMDAAGPQHMNLKLTRSKLESLVGDLIKRTIQPCQKALS 371
            ||||||||||.||||:||||:||||:|.||.||:|||:|:||:||||||.||:.|:|:|.:.||.
E. coli   261 RLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQ 325

  Fly   372 DAEVSKSEIGEVLLVGGMTRMPKVQSTVQELFGRQPSRSVNPDEAVAVGAAVQGGVLAGDVTDVL 436
            ||.:|.|:|.:|:||||.||||.||..|.|.||::|.:.||||||||:|||||||||.|||.|||
E. coli   326 DAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVL 390

  Fly   437 LLDVTPLSLGIETLGGVFTRLISRNTTIPTKKSQVFSTASDGQTQVEIKVHQGEREMANDNKLLG 501
            |||||||||||||:|||.|.||::|||||||.|||||||.|.|:.|.|.|.||||:.|.|||.||
E. coli   391 LLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLG 455

  Fly   502 SFTLVGIPPAPRGVPQIEVVFDIDANGIVHVSAKDKGTGKEQQIVIQSSGGLSKDEIENMIKKAE 566
            .|.|.||.|||||:|||||.|||||:||:|||||||.:||||:|.|::|.||::|||:.|::.||
E. coli   456 QFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAE 520

  Fly   567 EYATADKQKRELIEIVNQGESIVHDTETKMEEFKSQLPAEECEKLKKEIADLRTLLANKETADLE 631
            ..|.||::..||::..|||:.::|.|..::||...:|||::...::..:..|.|.|..::.|.:|
E. coli   521 ANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIE 585

  Fly   632 EVRKATSSLQQSSLKLFELAYKKMSAERESNAGAGSSDSSSSSDTSGEAKKEE 684
               .....|.|.|.||.|:|.::.:  ::..|||.:|.:::..|...:|:.||
E. coli   586 ---AKMQELAQVSQKLMEIAQQQHA--QQQTAGADASANNAKDDDVVDAEFEE 633

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-5	NP_001286414.1	dnaK	51..666	CDD:234715	380/618 (61%)
		HSPA9-like_NBD	51..428	CDD:212683	252/380 (66%)
		Syntaphilin	<566..>642	CDD:291936	22/75 (29%)
dnaK	NP_414555.1	dnaK	1..638	CDD:234715	386/638 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	680	1.000	Domainoid score	I2
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	1	1.000	-	-
Inparanoid	1	1.050	719	1.000	Inparanoid score	I5
OMA	1	1.010	-	-		QHG61239
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	1	1.000	-	-		oto111694
orthoMCL	1	0.900	-	-		OOG6_100423
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1906
SwiftOrtho	0	0.000	Not matched by this tool.
	10	9.770