Protein Alignment Hsc70-5 and Hspa5

DIOPT Version :10

• Sequence 1: NP_523741.2
• Gene: Hsc70-5 / 36583
• FlyBaseID: FBgn0001220
• Length: 686
• Species: Drosophila melanogaster
• Sequence 2: NP_037215.1
• Gene: Hspa5 / 25617
• RGDID: 2843
• Length: 654
• Species: Rattus norvegicus
• Alignment Length: 644
• Identity: 318/644 - (49%)
• Similarity: 430/644 - (66%)
• Gaps: 29/644 - (4%)

  Fly    52 GAVIGIDLGTTNSCLAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTF 116
            |.|:|||||||.||:.|.:..:.::|.|.:|.|.|||:||||.:||||:|..||.|..:|..||.
  Rat    28 GTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTV 92

  Fly   117 YATKRLIGRRFDDPEVKKDITNLSYKVVKASNG---DAWVSSTDGKVYSPSQIGAFILMKMKETA 178
            :..||||||.::||.|::||..|.:|||:....   ...:.....|.::|.:|.|.:|.||||||
  Rat    93 FDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETA 157

  Fly   179 EAYLNTPVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVINEPTAAALAYGMDKTE-DKIIAV 242
            ||||...|.:|||||||||||:|||||||||.||||||:|:||||||||:|||:||.| :|.|.|
  Rat   158 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILV 222

  Fly   243 YDLGGGTFDISILEIQKGVFEVKSTNGDTLLGGEDFDNHIVNFLVAEFKKDSGIDIRKDNIAMQR 307
            :||||||||:|:|.|..|||||.:|||||.|||||||..::...:..:||.:|.|:||||.|:|:
  Rat   223 FDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQK 287

  Fly   308 LKEAAEKAKCELSSSQQTDINLPYLTMDAAGPQHMNLKLTRSKLESLVGDLIKRTIQPCQKALSD 372
            |:...||||..|||..|..|.:......    :..:..|||:|.|.|..||.:.|::|.||.|.|
  Rat   288 LRREVEKAKRALSSQHQARIEIESFFEG----EDFSETLTRAKFEELNMDLFRSTMKPVQKVLED 348

  Fly   373 AEVSKSEIGEVLLVGGMTRMPKVQSTVQELF-GRQPSRSVNPDEAVAVGAAVQGGVLAG--DVTD 434
            :::.||:|.|::||||.||:||:|..|:|.| |::|||.:|||||||.|||||.|||:|  |..|
  Rat   349 SDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGD 413

  Fly   435 VLLLDVTPLSLGIETLGGVFTRLISRNTTIPTKKSQVFSTASDGQTQVEIKVHQGEREMANDNKL 499
            ::||||.||:|||||:|||.|:||.|||.:||||||:||||||.|..|.|||::|||.:..||.|
  Rat   414 LVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHL 478

  Fly   500 LGSFTLVGIPPAPRGVPQIEVVFDIDANGIVHVSAKDKGTGKEQQIVI-QSSGGLSKDEIENMIK 563
            ||:|.|.||||||||||||||.|:||.|||:.|:|:|||||.:.:|.| .....|:.:|||.|:.
  Rat   479 LGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVN 543

  Fly   564 KAEEYATADKQKRELIEIVNQGESIVHDTETKM---EEFKSQLPAEECEKLKKEIADLRTLLANK 625
            .||::|..||:.:|.|:..|:.||..:..:.::   |:...:|..|:.|.::|.:.:....|.:.
  Rat   544 DAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESH 608

  Fly   626 ETADLEEVRKATSSLQQSSLKLFELAYKKMSAERESNAGAGSSDSSSSSDTSGEAKKEE 684
            :.||:|:.:.....|::....:....|           |:|....:...|||   :|:|
  Rat   609 QDADIEDFKAKKKELEEIVQPIISKLY-----------GSGGPPPTGEEDTS---EKDE 653

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-5	NP_523741.2	dnaK	51..666	CDD:234715	312/624 (50%)
Hspa5	NP_037215.1	Required for interaction with ELAPOR1. /evidence=ECO:0000250|UniProtKB:P11021	1..80		28/51 (55%)
		HSP70	30..635	CDD:394970	309/608 (51%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250|UniProtKB:P11021	125..280		92/154 (60%)
		Interdomain linker. /evidence=ECO:0000250|UniProtKB:G3I8R9	409..419		4/9 (44%)
		Substrate-binding (SBD). /evidence=ECO:0000250|UniProtKB:P11021	420..500		56/79 (71%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	631..654		8/37 (22%)
		Prevents secretion from ER	651..654		2/3 (67%)