DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsc70-5 and Hspa5

DIOPT Version :9

Sequence 1:	NP_001286414.1	Gene:	Hsc70-5 / 36583	FlyBaseID:	FBgn0001220	Length:	686	Species:	Drosophila melanogaster
Sequence 2:	NP_001156906.1	Gene:	Hspa5 / 14828	MGIID:	95835	Length:	655	Species:	Mus musculus

Alignment Length:	644	Identity:	318/644 - (49%)
Similarity:	431/644 - (66%)	Gaps:	29/644 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly    52 GAVIGIDLGTTNSCLAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTF 116
            |.|:|||||||.||:.|.:..:.::|.|.:|.|.|||:||||.:||||:|..||.|..:|..||.
Mouse    29 GTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTV 93

  Fly   117 YATKRLIGRRFDDPEVKKDITNLSYKVVKASNG---DAWVSSTDGKVYSPSQIGAFILMKMKETA 178
            :..||||||.::||.|::||..|.:|||:....   ...:.....|.::|.:|.|.:|.||||||
Mouse    94 FDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETA 158

  Fly   179 EAYLNTPVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVINEPTAAALAYGMDKTE-DKIIAV 242
            ||||...|.:|||||||||||:|||||||||.||||||:|:||||||||:|||:||.| :|.|.|
Mouse   159 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILV 223

  Fly   243 YDLGGGTFDISILEIQKGVFEVKSTNGDTLLGGEDFDNHIVNFLVAEFKKDSGIDIRKDNIAMQR 307
            :||||||||:|:|.|..|||||.:|||||.|||||||..::...:..:||.:|.|:||||.|:|:
Mouse   224 FDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQK 288

  Fly   308 LKEAAEKAKCELSSSQQTDINLPYLTMDAAGPQHMNLKLTRSKLESLVGDLIKRTIQPCQKALSD 372
            |:...||||..|||..|..|.:......    :..:..|||:|.|.|..||.:.|::|.||.|.|
Mouse   289 LRREVEKAKRALSSQHQARIEIESFFEG----EDFSETLTRAKFEELNMDLFRSTMKPVQKVLED 349

  Fly   373 AEVSKSEIGEVLLVGGMTRMPKVQSTVQELF-GRQPSRSVNPDEAVAVGAAVQGGVLAG--DVTD 434
            :::.||:|.|::||||.||:||:|..|:|.| |::|||.:|||||||.|||||.|||:|  |..|
Mouse   350 SDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGD 414

  Fly   435 VLLLDVTPLSLGIETLGGVFTRLISRNTTIPTKKSQVFSTASDGQTQVEIKVHQGEREMANDNKL 499
            ::||||.||:|||||:|||.|:||.|||.:||||||:||||||.|..|.|||::|||.:..||.|
Mouse   415 LVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHL 479

  Fly   500 LGSFTLVGIPPAPRGVPQIEVVFDIDANGIVHVSAKDKGTGKEQQIVI-QSSGGLSKDEIENMIK 563
            ||:|.|.||||||||||||||.|:||.|||:.|:|:|||||.:.:|.| .....|:.:|||.|:.
Mouse   480 LGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVN 544

  Fly   564 KAEEYATADKQKRELIEIVNQGESIVHDTETKM---EEFKSQLPAEECEKLKKEIADLRTLLANK 625
            .||::|..||:.:|.|:..|:.||..:..:.::   |:...:|.:|:.|.::|.:.:....|.:.
Mouse   545 DAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESH 609

  Fly   626 ETADLEEVRKATSSLQQSSLKLFELAYKKMSAERESNAGAGSSDSSSSSDTSGEAKKEE 684
            :.||:|:.:.....|::....:....|           |:|....:...|||   :|:|
Mouse   610 QDADIEDFKAKKKELEEIVQPIISKLY-----------GSGGPPPTGEEDTS---EKDE 654

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-5	NP_001286414.1	dnaK	51..666	CDD:234715	312/624 (50%)
		HSPA9-like_NBD	51..428	CDD:212683	204/380 (54%)
		Syntaphilin	<566..>642	CDD:291936	19/78 (24%)
Hspa5	NP_001156906.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..81		28/51 (55%)
		HSP70	31..636	CDD:365808	309/608 (51%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	126..281		92/154 (60%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	410..420		4/9 (44%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	421..501		56/79 (71%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..655		8/37 (22%)
		Prevents secretion from ER	652..655		2/3 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810