Protein Alignment AGO1 and AGO2

DIOPT Version :10

• Sequence 1: NP_725341.1
• Gene: AGO1 / 36544
• FlyBaseID: FBgn0262739
• Length: 984
• Species: Drosophila melanogaster
• Sequence 2: NP_174413.2
• Gene: AGO2 / 840016
• AraportID: AT1G31280
• Length: 1014
• Species: Arabidopsis thaliana
• Alignment Length: 1028
• Identity: 310/1028 - (30%)
• Similarity: 479/1028 - (46%)
• Gaps: 166/1028 - (16%)

  Fly    41 ESQWTPSP------------TRPQ---SPSQAQTSFDTLT-----------SPPAPGSSVNPTAV 79
            :||..|.|            .|||   .||||..|:....           .|..|..|.:|:..
plant    67 QSQRGPPPGHGGRGTTQFQQPRPQVAPQPSQAPASYAGSVGGVAGRGAWGRKPQVPSDSASPSTS 131

  Fly    80 TSPSAQNVAAGGATVAGAAATAAQVASALGATTGSVTPAIATAT-----PATQPDMPVFTCPRRP 139
            |            ||.......|:|.        ::.|::..||     |..:||.......||.
plant   132 T------------TVVSEPVRVAEVM--------NLKPSVQVATSDRKEPMKRPDRGGVVAVRRV 176

  Fly   140 NLGREGRPIVLRANHFQVTM-PRGYVHHYDINIQPDKCPRKVNR-EIIETMVHAYSKI------- 195
            |         |..||::|.. |...:.|||:.|:.:...:||:| |:  .||.  .|:
plant   177 N---------LYVNHYKVNFNPESVIRHYDVEIKGEIPTKKVSRFEL--AMVR--DKVFTDNPDE 228

  Fly   196 FGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEALEGRT 260
            |.:....:||:.|:::...||.|:.::|...|  .|.:.|.:..|||....:.|.:|:|.:.||:
plant   229 FPLAMTAYDGQKNIFSAVELPTGSYKVEYPKT--EEMRGRSYTFTIKQVNVLKLGDLKEYMTGRS 291

  Fly   261 RQIPYDAILALDVVMRHLPSMTYTPVGRSFFS--------------SPEGYYHPLGGGREVWFGF 311
            ...|.|.:..:||||:..||.....||:|||:              :.:||.|.|
plant   292 SFNPRDVLQGMDVVMKEHPSKCMITVGKSFFTRETEPDEDFRFGVIAAKGYRHTL---------- 346

  Fly   312 HQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKI 376
                :|:...:.|.:|.|..||.||..||:::....:..|:.:.|       |....:|:.|||:
plant   347 ----KPTAQGLSLCLDYSVLAFRKAMSVIEYLKLYFNWSDMRQFR-------RRDVEEELIGLKV 400

  Fly   377 EITHCGQMRRKYRVCNVTRRPAQMQSFPL--QLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQV 439
            .:.| .:.::|..:..::.:..:...|.|  |..|....:.::.:||..||...:.:..:|||.:
plant   401 TVNH-RKNKQKLTIVGLSMQNTKDIKFDLIDQEGNEPPRKTSIVEYFRIKYGRHIVHKDIPCLDL 464

  Fly   440 GQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAP-DREREINNLVK-RADFNNDSY 502
            |:..:..::|:|.|::|.||...|...|..::..:|..:...| .|:|.|:.::| |...:....
plant   465 GKNGRQNFVPMEFCDLVEGQIYPKDNLDKDSALWLKKLSLVNPQQRQRNIDKMIKARNGPSGGEI 529

  Fly   503 VQEFGLTISNSMMEVRGRVLPPP--KLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQ 565
            :..|||.:..:|..|.||||..|  ||...|||    ..::..|.||       ||  |::..|.
plant   530 IGNFGLKVDTNMTPVEGRVLKAPSLKLAERGRV----VREEPNPRQN-------NQ--WNLMKKG 581

  Fly   566 FFTGVEIRIWAIACF-APQR--TVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEP 627
            ...|..::.||:..| |.:|  .:..|.:.|...:..::......||:.:....:..:..:.:|.
plant   582 VTRGSIVKHWAVLDFTASERFNKMPNDFVDNLIDRCWRLGMQMEAPIVYKTSRMETLSNGNAIEE 646

  Fly   628 MFRYL------KITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSN 686
            :.|.:      |.......||:..:..|...|..:|.:.:|.||:.|||.......|...|..:|
plant   647 LLRSVIDEASRKHGGARPTLVLCAMSRKDDGYKTLKWIAETKLGLVTQCFLTGPATKGGDQYRAN 711

  Fly   687 LCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMD-AHPSRYA 750
            |.||:|.|:||.|..|:.:.......:.|:|:||||.||.|.|...|||.||||::: ...:|||
plant   712 LALKMNAKVGGSNVELMDTFSFFKKEDEVMFIGADVNHPAARDKMSPSIVAVVGTLNWPEANRYA 776

  Fly   751 ATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREA 815
            |.|..|.||:|.||.......||:....::| |.:|::|:::|||||:.||..||..||..::
plant   777 ARVIAQPHRKEEIQGFGDACLELVKAHVQAT-GKRPNKIVIFRDGVSDAQFDMVLNVELLDVK-- 838

  Fly   816 CIKLEPE-YRPGITFIVVQKRHHTRLFCAEKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQ 879
             :..|.. |.|.||.||.||||.||.|.|...:.|.| ||:|:||.||..:.||.|:|||||||.
plant   839 -LTFEKNGYNPKITVIVAQKRHQTRFFPATNNDGSDK-GNVPSGTVVDTKVIHPYEYDFYLCSHH 901

  Fly   880 GIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR-YHLV 943
            |..|||:|:||:.|||:..|.||::|.|.:::|.|:.|||:.||:..|.|||.:||||.| ||..
plant   902 GGIGTSKPTHYYTLWDELGFTSDQVQKLIFEMCFTFTRCTKPVSLVPPVYYADMVAFRGRMYHEA 966

  Fly   944 ---EKEHDSGEGSHQSGCS----------EDRTPGAMARAITVHADTKKVMYF 983
               ||......|:..|..|          ||:.      ...:||:.:.||:|
plant   967 SSREKNFKQPRGASTSAASLASSLSSLTIEDKA------IFKLHAELENVMFF 1013

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO1	NP_725341.1	ArgoN	146..275	CDD:465134	39/137 (28%)
		ArgoL1	285..335	CDD:462567	15/63 (24%)
		PAZ_argonaute_like	336..456	CDD:239212	27/121 (22%)
		Piwi_ago-like	502..942	CDD:240015	169/453 (37%)
AGO2	NP_174413.2	ArgoN	237..306	CDD:465134	22/70 (31%)
		ArgoL1	316..366	CDD:462567	15/63 (24%)
		PAZ	371..488	CDD:460472	28/124 (23%)
		Piwi_ago-like	528..963	CDD:240015	168/452 (37%)