Protein Alignment AGO1 and Piwil4

DIOPT Version :10

• Sequence 1: NP_725341.1
• Gene: AGO1 / 36544
• FlyBaseID: FBgn0262739
• Length: 984
• Species: Drosophila melanogaster
• Sequence 2: NP_001423492.1
• Gene: Piwil4 / 689972
• RGDID: 1311015
• Length: 848
• Species: Rattus norvegicus
• Alignment Length: 942
• Identity: 219/942 - (23%)
• Similarity: 379/942 - (40%)
• Gaps: 208/942 - (22%)

  Fly    62 DTLTSPPAPG---SSVNPTAVTSPSAQNVAAGGA--TVAGAAATAAQVASALGATTGSVTPAIAT 121
            |.:.:..:||   :|...:.::.|....|::|..  |:........|....||..|
  Rat    27 DLMVTSVSPGDNEASSRTSVISQPHELGVSSGDGRRTLVEKRGNGRQDFVDLGVCT--------- 82

  Fly   122 ATPATQPDMPVFTCPRRPNLGREGRPIVLRANHFQVTMPRGY-VHHYDINIQPDKCPRKV----- 180
                    ....|..:....|..|.|:.|..|.|.:.:|:.: ::.|.:...||...|::
  Rat    83 --------REKLTHVKDSKTGSSGIPVQLATNLFHLDLPQDWQLYQYHVTYSPDLASRRLRTALL 139

  Fly   181 -NREIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVT-LPGE-GKDRIFRVTIK 242
             |..|:.....|           ||| .:|:.       :|:|:.:|| |..| .:....::|:.
  Rat   140 YNHSILSDKAKA-----------FDG-TSLFL-------SEKLDHKVTELTSETQRGETVKITLT 185

  Fly   243 WQAQ--------VSLFNLEEALEGRTRQIPYDAILALDVVMRHL-PSMTYTPVGRSFFSSPEGYY 298
            ..::        :..||                     |:.|.: ..::...:||:::...|...
  Rat   186 LTSELFPNSPMCIQFFN---------------------VIFRKIFKKLSMYQIGRNYYKPSEPVE 229

  Fly   299 HP---LGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKA---QPVIDFMCEVLDIRDINEQRK 357
            .|   |    .:|.||..||...:.|::.|.||:    ||.   :.|::||.::....|::
  Rat   230 IPQYKL----SLWPGFAISVSRFESKLLFNADVN----YKVLRNETVLEFMTDLCLRTDMS---- 282

  Fly   358 PLTDSQRVKFT----KEIKGLKIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVA 418
                    .||    |::.|| |.:|...  .:.||:.::........:|  |..:|.  |.|..
  Rat   283 --------CFTETCHKQLVGL-IVLTRYN--NKTYRIDDIDWSVKPTHAF--QKRDGS--ETTYV 332

  Fly   419 KYFLDKYRMKLRYPHLPCL--------QVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTS--TM 473
            .|:..:|.:.|...:.|.|        ....|.:..:|..|:|.:..       ||...||  .:
  Rat   333 DYYKQQYDITLSDLNQPVLVSLLKRRRNDNSEPQMVHLIPELCFLTG-------LTSQATSDFRL 390

  Fly   474 IKAT---------------ARSAPDREREINNLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLP 523
            :||.               ||.|.|.:|   |.|.|  |..:::...||     |.:.:.|||:|
  Rat   391 MKAVAEETRLSPMGRQQQLARLADDIQR---NQVTR--FELETWGLHFG-----SQLSLTGRVVP 445

  Fly   524 PPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVW--DMRGKQFFTGVEIRIWAIACFAPQRTV 586
            ..|:     :....|.|..|...            |  |||..:..:...:..|.|.|.:.
  Rat   446 SEKI-----LLQDHTCQPAFAAD------------WSKDMRSCKVLSSEPLNTWLIVCCSR---- 489

  Fly   587 REDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKI-TFPGLQLVVVVLP-GK 649
            .|..:.:|...|:::....|.. :|.|...|....|   ....|.::: ..|.:|||:.:|| .:
  Rat   490 AEHLIESFLSCLRRVGGSTGFS-VGHPKIIKVDENP---AAFLRAIQLHVNPDVQLVMCILPSNQ 550

  Fly   650 TPVYAEVKRVGDTVLGMATQCVQAKNVNKTSP--QTLSNLCLKINVKLGG-INSILVPSIRPKVF 711
            ...|..:||...:...:.:|||..:.:||...  ...:.:.:::..|||| :.::.:|.
  Rat   551 KNYYDSIKRYLSSDCPVPSQCVLTRTLNKQGMMLSVATKIAMQMTCKLGGELWAVEIPL------ 609

  Fly   712 NEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIM 776
             :.::.:|.|:.....  :|..::...|.|:::..:|:.:...:|:...:|...|...:...|..
  Rat   610 -KSLMVVGIDICRDAL--SKDVAVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNK 671

  Fly   777 FYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRP-GITFIVVQKRHHTRL 840
            :||..... |.|||.|||||..||...||::|:..:..:..:...:.|. .::.:||:||...||
  Rat   672 WYKHNHDL-PARIIAYRDGVGNGQLKAVLEYEVPQLLSSVTECGSDARSCRLSVVVVRKRCLLRL 735

  Fly   841 FCAEKKEQSGKS-GNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDEL 904
            |.     :||.: .|.|.||.||...|.|..:||||.|....:||..|::|:|::|||....|.:
  Rat   736 FA-----ESGHTLQNPPLGTVVDSEATRPEWYDFYLTSQTANRGTVSPTYYNVIYDDNALKPDHM 795

  Fly   905 QCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAF 936
            |.||::|||.|......:|:|||..|||.:.|
  Rat   796 QRLTFKLCHLYYNWQGVISVPAPCQYAHKLTF 827

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO1	NP_725341.1	ArgoN	146..275	CDD:465134	26/145 (18%)
		ArgoL1	285..335	CDD:462567	14/52 (27%)
		PAZ_argonaute_like	336..456	CDD:239212	27/134 (20%)
		Piwi_ago-like	502..942	CDD:240015	119/444 (27%)
Piwil4	NP_001423492.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..20
		PAZ_piwi_like	265..381	CDD:239211	27/141 (19%)
		Piwi_piwi-like_Euk	389..831	CDD:240016	130/489 (27%)