Protein Alignment AGO1 and Piwil2

DIOPT Version :10

• Sequence 1: NP_725341.1
• Gene: AGO1 / 36544
• FlyBaseID: FBgn0262739
• Length: 984
• Species: Drosophila melanogaster
• Sequence 2: NP_001351250.1
• Gene: Piwil2 / 57746
• MGIID: 1930036
• Length: 1042
• Species: Mus musculus
• Alignment Length: 1054
• Identity: 252/1054 - (23%)
• Similarity: 412/1054 - (39%)
• Gaps: 241/1054 - (22%)

  Fly    11 GPAQLHPH---TLPLTFPDLQMT-----STVGIIGK--VYESQWTPSPTRPQSPSQAQT------ 59
            ||..:||.   .:|..:|.....     ...|..|:  |:......||  |..|.|..:
Mouse    81 GPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSP--PLQPVQKDSVGLVSM 143

  Fly    60 ----SFDTLTSPPAP-------------GSSVN---------PTAVTSPSAQNVAAGGATVA--- 95
                ..||...||:.             |.|.|         .:::..||.  :|||.:.:|
Mouse   144 FRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSV--LAAGDSKLAEAS 206

  Fly    96 -------------------GAAATAA-----QVASALGATTGSVTPAIATATPATQPDM------ 130
                               |.||::.     :..||.|.|...  |......||..|..
Mouse   207 VGWSRMLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARD--PPRLPQPPALSPTSLHSADP 269

  Fly   131 -PVFTCPRRP-----NLGREGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVNREIIETMV 189
             ||.|..|:.     ..|.:|.|..|..|..::......|:.|.:...|.              |
Mouse   270 PPVLTMERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPS--------------V 320

  Fly   190 HAYSKIFGVLK---------PVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQA 245
            ...|..||:||         ..||| :.||    ||:   :|:..|.|..:.|.....::||.|
Mouse   321 ECKSMRFGMLKDHQSVTGNVTAFDG-SILY----LPV---KLQQVVELKSQRKTDDAEISIKIQ- 376

  Fly   246 QVSLFNLEEALEGRTRQ-IPYDAILALDVVMRH-LPSMTYTPVGRSFFSSPEG---YYHPLGGGR 305
                  |.:.||..:.. ||:     .:||.|. :..:....|||:|:.....   ..|.|
Mouse   377 ------LTKILEPCSDLCIPF-----YNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRL---- 426

  Fly   306 EVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKE 370
            ::|.|:..|:|.:...:.|..|||.... :...|:|.|      ..|.:|.|.       .|..|
Mouse   427 QIWPGYAASIRRTDGGLFLLADVSHKVI-RNDSVLDVM------HAIYQQNKE-------HFQDE 477

  Fly   371 IKGL---KIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYP 432
            ...|   .|.||...  .|.||:.:|........||  .:.:|:  |.|..:|:...|.:.::..
Mouse   478 CSKLLVGSIVITRYN--NRTYRIDDVDWNKTPKDSF--VMSDGK--EITFLEYYSKNYGITVKED 536

  Fly   433 HLPCLQVGQEHKHTYLPLEVCN----IVAGQRCIKKLTDMQTSTMI----KATARSAPDREREIN 489
            ..|.|        .:.|.|..|    ::.|:  |..|.::...|.|    |...|:..|..::||
Mouse   537 DQPLL--------IHRPSERQNNHGMLLKGE--ILLLPELSFMTGIPEKMKKDFRAMKDLTQQIN 591

  Fly   490 -----------NLVKRADFNN--DSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQ 541
                       .|::|...|.  .:.:..:||::...:.::.||:||..::..  |.::.:|.:.
Mouse   592 LSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINL--RNTSFVTSED 654

  Fly   542 LFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRIWAIACFAPQRTVREDALRNFTQQLQKISNDAG 606
            |    |.|...:        |.....| :.:..||:  |.|:|.:  |..|.....|:||:...|
Mouse   655 L----NWVKEVT--------RDASILT-IPMHFWAL--FYPKRAM--DQARELVNMLEKIAGPIG 702

  Fly   607 MPIIGQPCFCKYATGPDQVEPMFRYLK--ITFPG-LQLVVVVLPG-KTPVYAEVKRVGDTVLGMA 667
            |. |..|.:.:..  .|::|...|.::  :...| :|:||.::.| :..:|..:|::......:.
Mouse   703 MR-ISPPAWVELK--DDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVP 764

  Fly   668 TQCVQAKNVNKTSPQTLSNLCLKI----NVKLGG-INSILVPSIRPKVFNEPVIFLGADVTHPPA 727
            :|.:..:.:.:  |..|.::..||    |.|||| :..:.:|.       :.::.:|.||.|.|:
Mouse   765 SQVINVRTIGQ--PTRLRSVAQKILLQMNCKLGGELWGVDIPL-------KQLMVIGMDVYHDPS 820

  Fly   728 GDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRIILY 792
            ...:  |:...|.|::...:::.:.|..|...|||:..|...:...|..:|: .....|.:|::|
Mouse   821 RGMR--SVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYE-VNHCLPEKIVVY 882

  Fly   793 RDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQSGKSGNIPA 857
            |||||:||...|..:|:..::: |.:....|.|.:...||||:..|.|:.|........|    .
Mouse   883 RDGVSDGQLKTVANYEIPQLQK-CFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPS----P 942

  Fly   858 GTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSV 922
            ||.||..||.....||||.:|...||...|:||..:.:..:...|.:|.||::|||.|.....::
Mouse   943 GTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTI 1007

  Fly   923 SIPAPAYYAHLVAF 936
            .:|||..|||.:||
Mouse  1008 RVPAPCKYAHKLAF 1021

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO1	NP_725341.1	ArgoN	146..275	CDD:465134	32/138 (23%)
		ArgoL1	285..335	CDD:462567	14/52 (27%)
		PAZ_argonaute_like	336..456	CDD:239212	28/126 (22%)
		Piwi_ago-like	502..942	CDD:240015	119/444 (27%)
Piwil2	NP_001351250.1	PHA03247	<73..271	CDD:223021	38/195 (19%)
		ArgoL1	408..455	CDD:462567	14/51 (27%)
		PAZ_piwi_like	456..573	CDD:239211	32/145 (22%)
		Piwi_piwi-like_Euk	581..1025	CDD:240016	126/480 (26%)