Protein Alignment AGO1 and piwil1

DIOPT Version :10

• Sequence 1: NP_725341.1
• Gene: AGO1 / 36544
• FlyBaseID: FBgn0262739
• Length: 984
• Species: Drosophila melanogaster
• Sequence 2: NP_899181.1
• Gene: piwil1 / 368200
• ZFINID: ZDB-GENE-030813-2
• Length: 858
• Species: Danio rerio
• Alignment Length: 955
• Identity: 227/955 - (23%)
• Similarity: 389/955 - (40%)
• Gaps: 201/955 - (21%)

  Fly    48 PTRP-QSPSQAQTSFDTLTSPPAPGSSVN-------PTAVTSPSAQNVAAGGATV-AGAAATAAQ 103
            |..| ..|..:|.:...:.|.|:.|..|.       |.|::..:...::||...| .|......:
Zfish    18 PAAPGAQPPVSQEAAKPVVSTPSEGQLVGRGRQKPAPGAMSEEAMLQISAGFQQVKIGERGGRRR 82

  Fly   104 VASALGATTGSVTPAIATATPATQPDMPVFTCPRRPNLGREGRPIVLRANHFQ-VTMPRGYVHHY 167
            .....|..|..:...:                 :....|..|..|.||||..: ::.|...::.|
Zfish    83 DFHDSGVHTRQLMEHV-----------------KESKTGVSGTAIELRANFMRLLSRPMWALYQY 130

  Fly   168 DINIQPDKCPRKVNREII----ETMVHAYSKIFGVLKPVFDGR-----NNLYTRDPL----PIGN 219
            .::.:|....|::...::    ||:..|::         |||.     |.|...:.:    ....
Zfish   131 HVDYKPPMESRRLRSALLFQHEETLGKAHT---------FDGAILFLPNKLRNAETVLCSETRNG 186

  Fly   220 ERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEALEGRTRQIPYDAILALD----VVMRHLPS 280
            |::|:.|||                               |.::|..:.:.|.    :..|.|..
Zfish   187 EKVEITVTL-------------------------------TNELPPSSPVCLQFYNILFRRILRI 220

  Fly   281 MTYTPVGRSFFSSPEGY---YHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDF 342
            :....:||.:::..:.:   .|.|    .:|.||..::...:..:||..|||.... :::.|:||
Zfish   221 LNMQQIGRHYYNPDDPFNIPQHRL----TIWPGFMTTILQYESSIMLCSDVSHKVL-RSETVLDF 280

  Fly   343 MCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQL 407
            |..:.  :...:||.|      ...|||:.||.|...:   ..:.||:.::........:|
Zfish   281 MYSLR--QQCGDQRFP------EACTKELVGLIILTKY---NNKTYRIDDIAWDHTPNNTF---- 330

  Fly   408 ENGQTVECTVAKYFLDKYRMKL-------------------RYPHLPCLQVGQEHKHTYLPLEVC 453
            :.|.| |.:...||..:|.:.:                   |.|..|.:.|.          |.|
Zfish   331 KKGDT-EISFKNYFKSQYGLDITDGNQVLLVSHVKRLGPSGRPPPGPAMLVP----------EFC 384

  Fly   454 NIVAGQRCIKKLTDMQTSTM-----IKATARSAPD-REREINNLVKRADFNND--SYVQEFGLTI 510
            .:..       |||...:..     :.:..|.:|: ||..||.|:...:.|.|  :.:..:||:.
Zfish   385 YLTG-------LTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSF 442

  Fly   511 SNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVW--DMRGKQFFTGVEIR 573
            .|.::.:.|||||..::..|||...                .:|....|  :|||....:.:.:.
Zfish   443 ENKLLSLNGRVLPSERIIQGGRAFE----------------YNPWTADWSKEMRGLPLISCMSLD 491

  Fly   574 IWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFP- 637
            .|.:  |..:|..  |..::..|.|.|:|...|:. :.:....:|   .|:.|.:.|.|:....
Zfish   492 NWLM--FYTRRNA--DVAQSLLQTLNKVSGPMGIR-MQRAVMIEY---EDRQESLLRALQQNVAR 548

  Fly   638 GLQLVVVVLP-GKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTL----SNLCLKINVKLGG 697
            ..|:|||:|| .:...|..||:.........:|||.::.::|  ||.|    :.:.|::|.|:||
Zfish   549 ETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISK--PQALMTVATKIALQMNCKMGG 611

  Fly   698 -INSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQE 761
             :.|:.:|.       ..::.:|.|..|..|..  |.||.|:|.|::...||:.:...:|...||
Zfish   612 ELWSVEIPL-------RQLMIVGIDCYHDTAAG--KRSIGAMVASLNQGMSRWFSKCVLQNRGQE 667

  Fly   762 IIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPG 826
            ||..|...::..|..:.|..... |.|||:|||||.:|....|:.:|:..|.::...:..:|.|.
Zfish   668 IIDALKGSLQGALKAYLKYNNSL-PSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPK 731

  Fly   827 ITFIVVQKRHHTRLFCAEKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYH 891
            ::.:||:||..:|.|.    ...||..|.|.||.:|..:|.|..:||::.|.....|...|:||:
Zfish   732 LSVVVVKKRISSRFFA----RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYN 792

  Fly   892 VLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAF 936
            |::|::....|.:|.|||:|||.|......|.:|||..|||.:||
Zfish   793 VVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAF 837

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO1	NP_725341.1	ArgoN	146..275	CDD:465134	25/146 (17%)
		ArgoL1	285..335	CDD:462567	12/52 (23%)
		PAZ_argonaute_like	336..456	CDD:239212	28/138 (20%)
		Piwi_ago-like	502..942	CDD:240015	130/444 (29%)
piwil1	NP_899181.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..56		9/37 (24%)
		GAGE	17..104	CDD:461753	16/102 (16%)
		PAZ	275..412	CDD:198017	32/169 (19%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000250|UniProtKB:Q9JMB7	314..316		0/1 (0%)
		Piwi_piwi-like_Euk	398..841	CDD:240016	139/480 (29%)
		MID region. /evidence=ECO:0000250|UniProtKB:Q9JMB7	476..612		37/145 (26%)